BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1594.p (572 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311064|ref|YP_003775156.1| acetolactate synthase isozyme I... 1180 0.0 gi|300311584|ref|YP_003775676.1| pyruvate dehydrogenase [Herbasp... 164 2e-41 gi|300311743|ref|YP_003775835.1| acetolactate synthase [Herbaspi... 127 2e-30 gi|300310483|ref|YP_003774575.1| benzoylformate decarboxylase [H... 94 4e-20 >gi|300311064|ref|YP_003775156.1| acetolactate synthase isozyme III large subunit [Herbaspirillum seropedicae SmR1] Length = 572 Score = 1180 bits (3052), Expect = 0.0, Method: Compositional matrix adjust. Identities = 562/572 (98%), Positives = 570/572 (99%) Query: 1 MNAEHLSGADILVRCLAEEGVEHVFGYPGGAVLYIYDAIFKQDKFQHILVRHEQAAVHAA 60 M+AE L+GADILVRCLAEEGVEHVFGYPGGAVLYIYDAIFKQDKFQHILVRHEQAAVHAA Sbjct: 1 MSAEQLTGADILVRCLAEEGVEHVFGYPGGAVLYIYDAIFKQDKFQHILVRHEQAAVHAA 60 Query: 61 DAYSRSSQKVGVAIVTSGPGVTNAVTGLATAYMDSIPMVVISGQVPSTAIGQDAFQECDT 120 DAYSRSSQKVGVAIVTSGPGVTNAVTGLATAYMDSIPMVVISGQVPSTAIGQDAFQECDT Sbjct: 61 DAYSRSSQKVGVAIVTSGPGVTNAVTGLATAYMDSIPMVVISGQVPSTAIGQDAFQECDT 120 Query: 121 VGITRPCVKHNFLVKDVKDLAETVKKAFFIATTGRPGPVLVDIPKDITMHTCAYEYPKAV 180 VGITRPCVKHNFLVKDVKDLAETVKKAF+IATTGRPGPVLVDIPKDITMHTCAY YPKAV Sbjct: 121 VGITRPCVKHNFLVKDVKDLAETVKKAFYIATTGRPGPVLVDIPKDITMHTCAYAYPKAV 180 Query: 181 EMRSYKPVDKGHSGQIRKALQLLLTAERPMIYTGGGIILANAAPELNKLVDRLGYPCTNT 240 EMRSYKPVDKGHSGQIRKALQLLL+AERPMIYTGGGIILANAAPELNKLVDRLGYPCTNT Sbjct: 181 EMRSYKPVDKGHSGQIRKALQLLLSAERPMIYTGGGIILANAAPELNKLVDRLGYPCTNT 240 Query: 241 LMGLGAYRSSSDKFVGMPGMHGTFEANMAMQHCDVLIAIGARFDDRVIGNPKHFASHARK 300 LMGLGAYRSSSDKFVGMPGMHGT+EANMAMQHCDVLIAIGARFDDRVIGNPKHFASHARK Sbjct: 241 LMGLGAYRSSSDKFVGMPGMHGTYEANMAMQHCDVLIAIGARFDDRVIGNPKHFASHARK 300 Query: 301 IIHIDIDPSSISKRVKVDIPIVGNVKDVLQELLAQLDSAETRQNGAALDAWWKQINEWRK 360 IIHIDIDPSSISKRVKVDIPIVGNVKDVLQELL+QLD+AETRQNGAALDAWWKQINEWRK Sbjct: 301 IIHIDIDPSSISKRVKVDIPIVGNVKDVLQELLSQLDAAETRQNGAALDAWWKQINEWRK 360 Query: 361 RDCLKFATSDEFIKPQSVVQKVWEVTKGDAFITSDVGQHQMWAAQYYGFDKPRRWINSGG 420 RDCLKFATS+EFIKPQSVVQKVWEVTKGDAFITSDVGQHQMWAAQYYGFDKPRRWINSGG Sbjct: 361 RDCLKFATSEEFIKPQSVVQKVWEVTKGDAFITSDVGQHQMWAAQYYGFDKPRRWINSGG 420 Query: 421 LGTMGVGLPYAMGVQMANPDATVACITGEASIQMCIQELATCKQYHLTPKIILLNNRFLG 480 LGTMGVGLPYAMGVQMANPDATVACITGEASIQMCIQELATCKQYHLTPKIILLNNRFLG Sbjct: 421 LGTMGVGLPYAMGVQMANPDATVACITGEASIQMCIQELATCKQYHLTPKIILLNNRFLG 480 Query: 481 MVRQWQQIDYGSRYSESYMDSLPDFNKLAESYGHVGMKIEKPGDVDGALREAFAMKDRLV 540 MVRQWQQIDYGSRYSESYMDSLPDFNKLAESYGHVGMKIEKPGDVDGALREAFAMKDRLV Sbjct: 481 MVRQWQQIDYGSRYSESYMDSLPDFNKLAESYGHVGMKIEKPGDVDGALREAFAMKDRLV 540 Query: 541 FMNFITDQTENVWPMVKAGKGLTEMLLGSEDL 572 FMNFITDQTENVWPMVKAGKGLTEMLLGSEDL Sbjct: 541 FMNFITDQTENVWPMVKAGKGLTEMLLGSEDL 572 >gi|300311584|ref|YP_003775676.1| pyruvate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 564 Score = 164 bits (415), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 145/555 (26%), Positives = 237/555 (42%), Gaps = 24/555 (4%) Query: 9 ADILVRCLAEEGVEHVFGYPGGAVLYIYDAIFKQDKFQHILVRHEQAAVHAADAYSRSSQ 68 ADIL+ L E GV +FG G A+ + DAI + + + I VRHE+ A AA ++ + Sbjct: 6 ADILIDTLYEIGVRQIFGVVGDALNPLTDAIRRDKRIEWIGVRHEEGAALAAAGQAKLTG 65 Query: 69 KVGVAIVTSGPGVTNAVTGLATAYMDSIPMVVISGQVPSTAIGQDAFQECDTVGITRPCV 128 ++ V T+GPG + V GL A D P++ ISG VP+ G D QE + R Sbjct: 66 RLAVCCGTTGPGANHLVAGLYEARKDHAPVLAISGGVPAAHRGTDYLQENSPELLFRDVA 125 Query: 129 KHNFLVKDVKDLAETVKKAFFIATTGRPGPVLVDIPKDITMHTCAYEYPKAVEMRSYKPV 188 ++ ++ +A A R G ++IP D+ + P +R P+ Sbjct: 126 AYSQIITSPDQAPAVFHQAIAQAYAQR-GVAHLNIPADVIGAKTSGSVPSLHTLRERTPL 184 Query: 189 DKGHSGQIRKALQLLLTAERPMIYTGGGIILANAAPELNKLVDRLGYPCTNTLMGLGAYR 248 + I+ A L+ A I+ G G A E+ L +L P +T G Sbjct: 185 SP-PAADIQAAADLINAAGSVAIFAGNGC--RGAIDEVLALASKLQAPVMHTFRGKDLAS 241 Query: 249 SSSDKFVGMPGMHGTFEANMAMQHCDVLIAIGARFDDRVIGNPKHFASHARKIIHIDIDP 308 ++G G+ G A+ +V++ +G+ + F K+I ID Sbjct: 242 YDHPHWIGGVGLIGGAPGTDALHAAEVMLMLGSDYPY------SEFLPTKSKVIQIDERG 295 Query: 309 SSISKRVKVDIPIVGNVKDVLQELLAQLDSAETRQNGAALDAWWKQINEWRKRDCLKFAT 368 + +R V + I G+V +Q+LL ++ E + +GA L +Q N R+R K + Sbjct: 296 FVLGRRAPVTLGITGSVAPAVQQLLERV---EGKTDGAFL----QQANAHRERWNAKLDS 348 Query: 369 SDEF------IKPQSVVQKVWEVTKGDAFITSDVGQHQMWAAQYYGFDKPRRWINSGGLG 422 + E IKPQSV + + ++ DA D G +W + +R + S Sbjct: 349 AAEIKRHHKKIKPQSVARAISDLADDDAAFVVDTGVVTLWCGNWIRQRGGQRILASFNNA 408 Query: 423 TMGVGLPYAMGVQMANPDATVACITGEASIQMCIQELATCKQYHLTPKIILLNNRFLGMV 482 +G L A G+Q + + V G+ M + E T ++ L K+++ NNR G+V Sbjct: 409 AVGTSLGQANGIQALDRERQVVVAVGDGGFTMLLGEFMTSVEHRLPVKVVVFNNREWGLV 468 Query: 483 RQWQQIDYGSRYSESYMDSLPDFNKLAESYGHVGMKIEKPGDVDGALREAFAMKDRLVFM 542 + + G E PDF A + G G + P +++G L++ A V Sbjct: 469 -HLEMEEAGMPAFEGSEFPNPDFAAFARACGGQGFSVRTPEELEGGLQQLLAAPGPAVLD 527 Query: 543 NFITDQTENVWPMVK 557 FI V P +K Sbjct: 528 VFIDPDELPVMPHIK 542 >gi|300311743|ref|YP_003775835.1| acetolactate synthase [Herbaspirillum seropedicae SmR1] Length = 625 Score = 127 bits (319), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 142/578 (24%), Positives = 247/578 (42%), Gaps = 73/578 (12%) Query: 20 GVEHVFGYPG----GAVLYIYDAIFKQDKFQHILVRHEQAAVHAADAYSRSSQKVGVAIV 75 GV +FG+ G LY Y A + +EQA H+A AY+++ + + V Sbjct: 40 GVFSIFGHGNVAGMGEALYQYRATLPTYR-----AHNEQAMAHSAIAYAKAHLRQRMMAV 94 Query: 76 TS--GPGVTNAVTGLATAYMDSIPMVVISGQV-----PSTAIGQDAFQECDTVGIT---- 124 T+ GPG TN +T A A+++ +P++++ G V P + Q ++ + G++ Sbjct: 95 TTSIGPGATNLLTAAALAHVNRLPVLLLPGDVFVSRRPDPVLQQ--LEDFNDPGLSVNDA 152 Query: 125 -RPCVKHNFLVKDVKDLAETVKKAFFIAT-TGRPGPVLVDIPKDITMHTCAYEYP----- 177 RP + + + L + +A + T + GPV + +P+D+ T AY+YP Sbjct: 153 FRPLSRMFDRICYPEQLLTALPRAIQVLTDPAQCGPVTLALPQDV--QTMAYDYPLDFFS 210 Query: 178 -KAVEMRSYKPVDKGHSGQIRKALQLLLTAERPMIYTGGGIILANAAPELNKLVDRLGYP 236 + + R+ P + ++ A+ LL A +P++ GGG++ NA L + ++ G P Sbjct: 211 PRVIVPRAQAPAAQ----ELEDAVALLRQARQPLLIAGGGVLYGNACEHLRRFAEQHGVP 266 Query: 237 CTNTLMGLGAYRSSSDKFVGMPGMHGTFEANMAMQHCDVLIAIGARFDDRVIGNPKHFAS 296 T G A S +G G+ G+ AN Q DV+IA+G R D G+ FA Sbjct: 267 VAETQAGKSALPWSHALQMGAIGVTGSPAANALAQEADVVIAVGTRLQDFTTGSHTLFAQ 326 Query: 297 HARKIIHIDIDPSSISKRVKVDIPI-VGNVKDVLQELLAQLDSAETRQNGAALDAWWKQI 355 ++++++++ K + + G + L L SA A A Sbjct: 327 A--RLLNLNVNAMDAHKWRGLALQADAGLGLEALSHALEGWRSAPEWHARAHTLA----- 379 Query: 356 NEWRKRDCLKFATSD---------EFIKPQSVVQKVWEVTKGDAFI----TSDVGQHQMW 402 +WR+R +D E I ++ + V + D + T H++W Sbjct: 380 QDWRERVAQITGQTDTGGRLPYDGEVIG--AIQRSVADSPSQDIVVCAAGTLPAELHKLW 437 Query: 403 AAQYYGFDKPRRWINSGGLGTMGVGLPYAMGVQMANPDATVACITGEASIQMCIQELATC 462 A +P + G MG + +GV++A P V I G+ S M ELAT Sbjct: 438 RA-----GRPGAYHVEYGYSCMGYEVAGGLGVKLAQPQREVIVIVGDGSYLMMNSELATS 492 Query: 463 KQYHLTPKIILLNNRFLGMVRQWQQIDYGSRYSESYMDSLP--------DFNKLAESYGH 514 +++L+NR G + + QQ G+ ++ D L DF A S G Sbjct: 493 VMLGAKLIVVVLDNRGYGCINRLQQACGGAPFNNMLADCLQAGPGAPAIDFAAHARSLGA 552 Query: 515 VGMKIEKPGDVDGALREAFAMKDRLVFMNFITDQTENV 552 +G ++ +++ AL+ A A DR + TD + Sbjct: 553 LGENVKTITELEAALQRARA-ADRSYLVCIDTDASRTT 589 >gi|300310483|ref|YP_003774575.1| benzoylformate decarboxylase [Herbaspirillum seropedicae SmR1] Length = 532 Score = 93.6 bits (231), Expect = 4e-20, Method: Compositional matrix adjust. Identities = 131/531 (24%), Positives = 216/531 (40%), Gaps = 36/531 (6%) Query: 20 GVEHVFGYPGGAVLYIYDAIFKQDKFQHILVRHEQAAVHAADAYSRSSQKVGVAIVTSGP 79 G+ VF PG L ++ F +D F+++L E V AD ++++S + + S Sbjct: 23 GMTSVFANPGSTELPMFRD-FPED-FRYVLGLQEAVVVGMADGFAQASGNAALVNLHSAA 80 Query: 80 GVTNAVTGLATAYMDSIPMVVISGQVPSTAIGQDAF-QECDTVGITRPCVKHNFLVKDVK 138 GV NA+ + TA+ + P+++ +GQ + + D F + +P +K + Sbjct: 81 GVGNAMGNIFTAFRNRTPLIITAGQQARSILPFDPFLGATQATELAKPYLKWSIEPARAA 140 Query: 139 DLAETVKKAFFIATTGRPGPVLVDIPKDITMHTCAYEYPKAVEMRSYKPVDKGHSGQIRK 198 D+ + +A+ +A GPV V IP D P V R +P + Sbjct: 141 DVPLALARAYHMAMQEPRGPVFVSIPVDDWDQPAELVPPTQVTSRC-RPEPAAIARLAAA 199 Query: 199 ALQLLLTAERPMIYTGGGIILANAAPELNKLVDRL-GYPCTNTLMGLGAYRSSSDKFVG- 256 Q ++RP I G + A A L + L + ++ F G Sbjct: 200 LEQ----SQRPAIVVGAELDRAGAWDAAIALAETLRARVFVAPMSSRCSFPEDHPLFAGF 255 Query: 257 MPGMHGTFEANMAMQHCDVLIAIGA-RFDDRVIGNPKHFASHARKIIHIDIDPSSISKRV 315 +P M A+Q D+L A+GA F V G+ H A ID DP + S Sbjct: 256 LPAMREKIV--QALQGHDLLFALGAPAFTYHVEGSGPHVPPGAALFQLID-DPVTASW-T 311 Query: 316 KVDIPIVGNVKDVLQELLAQLDSAETRQNGAALDAWWKQINEWRKRDCLKFATSDEFIKP 375 IVGN++ L +LLAQ+ Q + E + +A Sbjct: 312 PTGQAIVGNIQLGLDDLLAQIRRQPPTQRAQPPARPARARVEASAPLSMAYA-------- 363 Query: 376 QSVVQKVWEVTK-GDAFITSDVGQHQMWAAQYYGFDKPRRW--INSGGLGTMGVGLPYAM 432 +Q + +V + GD + G + A + F +P + + SGGL G G+P A+ Sbjct: 364 ---LQTLADVREAGDIVVEEAPGSRPVMQA-HLPFTRPGSFYTMASGGL---GYGMPAAV 416 Query: 433 GVQMANPDATVACITGEASIQMCIQELATCKQYHLTPKIILLNNRFLGMVRQWQQIDYGS 492 GV +A P + V + G+ S Q + + Q L ++LNN ++ + + Sbjct: 417 GVALARPHSRVIGLIGDGSAMYSPQAIYSAVQLGLPITFVILNNGRYAALQDFAPVFGFG 476 Query: 493 RYSESYMDSLP--DFNKLAESYGHVGMKIEKPGDVDGALREAFAMKD-RLV 540 + LP DF LA S G G+++ + + ALREA + RLV Sbjct: 477 PEDPVHGTELPGLDFVALAHSMGCHGVRVSQAAALAPALREALCSNETRLV 527 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,815,730 Number of extensions: 76263 Number of successful extensions: 225 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 219 Number of HSP's successfully gapped: 4 Length of query: 572 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 482 Effective length of database: 1,193,720 Effective search space: 575373040 Effective search space used: 575373040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)