BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1589.p (176 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311060|ref|YP_003775152.1| transmembrane protein [Herbaspi... 259 1e-70 >gi|300311060|ref|YP_003775152.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 168 Score = 259 bits (661), Expect = 1e-70, Method: Compositional matrix adjust. Identities = 133/169 (78%), Positives = 147/169 (86%), Gaps = 1/169 (0%) Query: 1 MSELTTPSLRRRLSSMLYESMLLFGVLFMSGWLFSTLLQQRNALYLRHALEFWLFLVLGV 60 M +LTTPSLRRRLSSMLYESMLLFGVLF+SGWLFSTLLQQR+ALYLRH LE WLFLVL + Sbjct: 1 MPDLTTPSLRRRLSSMLYESMLLFGVLFISGWLFSTLLQQRHALYLRHGLEMWLFLVLAL 60 Query: 61 YFIWFWTHGGQTLAMKTWRFKVVDRAGQPLGRGRAALRFLLAWLWILPGLALASVLRGHP 120 YFIWFWTHGGQTLAMKTWRF+VVD+ G LG RAA RFLLAWLW+LPGLALA+ + G Sbjct: 61 YFIWFWTHGGQTLAMKTWRFRVVDQHGAALGLWRAAWRFLLAWLWVLPGLALAAAVHGQQ 120 Query: 121 WLLILIPAANIVLWALTSRLDPRGQFLHDRLAGTRLVDVIQAPKASTKT 169 W L+LIPA NIVLWALT+RLD GQFLHDRLAGTRLVDV A A++K+ Sbjct: 121 WTLVLIPAGNIVLWALTARLDKDGQFLHDRLAGTRLVDV-TAMAATSKS 168 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.329 0.140 0.460 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 512,782 Number of extensions: 18046 Number of successful extensions: 86 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 86 Number of HSP's successfully gapped: 1 Length of query: 176 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 97 Effective length of database: 1,245,805 Effective search space: 120843085 Effective search space used: 120843085 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 127 (53.5 bits)