BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1568.p (460 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311040|ref|YP_003775132.1| major facilitator superfamily p... 835 0.0 gi|300310856|ref|YP_003774948.1| major facilitator superfamily p... 497 e-142 gi|300313188|ref|YP_003777280.1| major facilitator superfamily p... 311 9e-86 gi|300312683|ref|YP_003776775.1| major facilitator superfamily p... 231 8e-62 gi|300312566|ref|YP_003776658.1| multidrug resistance protein pr... 114 2e-26 >gi|300311040|ref|YP_003775132.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 441 Score = 835 bits (2158), Expect = 0.0, Method: Compositional matrix adjust. Identities = 422/441 (95%), Positives = 431/441 (97%) Query: 20 MDATVLATSLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMADKLGARKVFSAAMI 79 MDATVLATSLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMADKLGARKVFSAAMI Sbjct: 1 MDATVLATSLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMADKLGARKVFSAAMI 60 Query: 80 VFMTGSILCAAAGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDKSELVKAVSYLTLPA 139 VFMTGSILCA +GSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDKSELVKAVSYLTLPA Sbjct: 61 VFMTGSILCAVSGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDKSELVKAVSYLTLPA 120 Query: 140 LLGPVVGPLLGGFITTYYHWRLVFLLNIPFCFLGLYLAHRFIENFKEDAPAPLDVKGFVL 199 LLGPVVGPLLGGFITTY+HWR VFL+NIPFCFLGLYLA RFI+NFKED PAPLDVKGFVL Sbjct: 121 LLGPVVGPLLGGFITTYFHWRWVFLINIPFCFLGLYLARRFIQNFKEDNPAPLDVKGFVL 180 Query: 200 TSVGASMIMLGLSLFGSPLVDGATPVAMCSIGAIALVMYYRHSTQIPYPLLDFRLLRIPT 259 TSVGA+MIMLGLSLFGS LVDGATPVAMCSIGAIALVMYYRHSTQI +PLLDFRLLRIPT Sbjct: 181 TSVGAAMIMLGLSLFGSHLVDGATPVAMCSIGAIALVMYYRHSTQIEFPLLDFRLLRIPT 240 Query: 260 LHASVVGGSLFRIGLGAVPFLLPLALQEGLGMTAFQAGSITCASAFGSLFIRLLASRVLR 319 LHASVVGGSLFRIGLGAVPFLLPLALQEGLGMTAFQAGSITCASAFGSLFIRLLA RVLR Sbjct: 241 LHASVVGGSLFRIGLGAVPFLLPLALQEGLGMTAFQAGSITCASAFGSLFIRLLAGRVLR 300 Query: 320 RYGFRTVLMVNAVFAGIALAAYGIFSLHTPYLLIFAIVLMGGVFPALQFTCLNSIAYADI 379 RYGFRTVLMVNAVFAGIALAAYGIFS+HTPYLLI +IVLMGGVFPALQFTCLNSIAYADI Sbjct: 301 RYGFRTVLMVNAVFAGIALAAYGIFSIHTPYLLIVSIVLMGGVFPALQFTCLNSIAYADI 360 Query: 380 DNRDAGRVTSLASVIQQLSLGLGVTVAGIVLQLSNHLQGHTDIVEADFWPAFLVIGLFSV 439 D+RDAGRVTSLASVIQQLSLGLGVTVAGIVLQLSNHLQGH IVEADFWPAFLVIGLFSV Sbjct: 361 DSRDAGRVTSLASVIQQLSLGLGVTVAGIVLQLSNHLQGHATIVEADFWPAFLVIGLFSV 420 Query: 440 ASVPISGKLPHNAGLELSRGK 460 ASVPISGKLPHNAGLELSRGK Sbjct: 421 ASVPISGKLPHNAGLELSRGK 441 >gi|300310856|ref|YP_003774948.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 446 Score = 497 bits (1279), Expect = e-142, Method: Compositional matrix adjust. Identities = 244/438 (55%), Positives = 326/438 (74%) Query: 20 MDATVLATSLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMADKLGARKVFSAAMI 79 MD+TV++TSLPALA D++ DPI+LKLA+T+Y+ +L VFIPISGWMAD+ G+ +F +A+ Sbjct: 1 MDSTVISTSLPALALDMNVDPISLKLALTSYLVSLAVFIPISGWMADRFGSLTIFRSAIC 60 Query: 80 VFMTGSILCAAAGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDKSELVKAVSYLTLPA 139 VFM GSILC + +L FVAARFLQGIGGAMMVPVGR++I RSV+K LVKA+SYLT+PA Sbjct: 61 VFMLGSILCGVSSTLPQFVAARFLQGIGGAMMVPVGRLVILRSVEKVNLVKALSYLTIPA 120 Query: 140 LLGPVVGPLLGGFITTYYHWRLVFLLNIPFCFLGLYLAHRFIENFKEDAPAPLDVKGFVL 199 L GPV+GP LGGFITTY+HWR +F +NIP LGLYLA RFI + K + APLD+KGF+L Sbjct: 121 LFGPVLGPPLGGFITTYFHWRWIFFINIPISILGLYLATRFIPDLKAEQVAPLDLKGFLL 180 Query: 200 TSVGASMIMLGLSLFGSPLVDGATPVAMCSIGAIALVMYYRHSTQIPYPLLDFRLLRIPT 259 +++G S++M+GL+ G LV G + G + L Y H+ + PL+D LL+IPT Sbjct: 181 SALGCSLLMMGLATAGRHLVSGEVALTCVLAGLVFLAAYVWHARRASAPLIDLALLKIPT 240 Query: 260 LHASVVGGSLFRIGLGAVPFLLPLALQEGLGMTAFQAGSITCASAFGSLFIRLLASRVLR 319 L A V GGS +R+G+GA+PFLLPL LQ G G T F +G +TCASA G++F++ +A+R LR Sbjct: 241 LRAGVFGGSFYRVGIGAIPFLLPLMLQLGFGYTPFHSGMLTCASAAGAMFMKTIAARFLR 300 Query: 320 RYGFRTVLMVNAVFAGIALAAYGIFSLHTPYLLIFAIVLMGGVFPALQFTCLNSIAYADI 379 RYGFR VL+VN + L A F+ TPYLL+ I+L+GG F +LQFT LN+I+YAD+ Sbjct: 301 RYGFRRVLLVNGALGVLGLWAIATFTPQTPYLLMVVILLIGGCFRSLQFTSLNAISYADV 360 Query: 380 DNRDAGRVTSLASVIQQLSLGLGVTVAGIVLQLSNHLQGHTDIVEADFWPAFLVIGLFSV 439 D + TS+ASV QQLS+G+GV +AG LQ+SN +QGH +V DFWPAFLV+G + Sbjct: 361 DKLRLSQATSMASVAQQLSIGMGVVLAGFALQMSNEIQGHATLVAQDFWPAFLVVGAVAA 420 Query: 440 ASVPISGKLPHNAGLELS 457 ++ + +LPH+AG E++ Sbjct: 421 CALLMLRQLPHDAGAEMA 438 >gi|300313188|ref|YP_003777280.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 311 bits (796), Expect = 9e-86, Method: Compositional matrix adjust. Identities = 179/460 (38%), Positives = 275/460 (59%), Gaps = 6/460 (1%) Query: 6 LIPLIVGCALFMENMDATVLATSLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMA 65 ++P IV FME +D T++AT+LP +A+ P L L MTAY+ L VFIPISGW+A Sbjct: 31 MLPYIVAATFFMEYLDTTIIATALPQMAQSFGVGPNELSLGMTAYMLTLAVFIPISGWVA 90 Query: 66 DKLGARKVFSAAMIVFMTGSILCAAAGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDK 125 D+ G+R VF A+ +F SILC + ++ F AAR LQG+GGAMMVPVGR+I+ RS K Sbjct: 91 DRFGSRTVFGMAIAIFTLASILCGFSQNVWEFTAARVLQGLGGAMMVPVGRMIVVRSTGK 150 Query: 126 SELVKAVSYLTLPALLGPVVGPLLGGFITTYYHWRLVFLLNIPFCFLGLYLAHRFIENFK 185 L+KA++ +T PA++ PVVGP +GGFITTY W +FL+N+PF + N Sbjct: 151 DRLMKAIATITWPAIVAPVVGPSIGGFITTYASWHWIFLMNVPFGLAAFTAVMMVVRNEH 210 Query: 186 EDAPAPLDVKGFVLTSVGASMIMLGLSLFGSPLVDGATPVAMCSIGAIALVMYYRHSTQI 245 + PLD+ GF+L+ + ++ G + + + ++G + +RH +I Sbjct: 211 GVSKRPLDLVGFLLSGAALTSLLYGTEIASHMESNIKVALGFVALGVVLGYGAWRHMQRI 270 Query: 246 PYPLLDFRLLRIPTLHASVVGGSLFRIGLGAVPFLLPLALQEGLGMTAFQAGSITCASAF 305 +PLLDF LRIPT +V+ GSL RIG+ AVP+L+PL Q G G+TAFQ+G + A+A Sbjct: 271 EHPLLDFSNLRIPTYSVTVLTGSLTRIGVDAVPYLMPLMFQIGFGLTAFQSGLLLLATAL 330 Query: 306 GSLFIRLLASRVLRRYGFRTVLMVNAVFAGIALAAYGIFSLHTPYLLIFAIVLMGGVFPA 365 G+L ++ +R+L+R+GFR+V + NA AG + A G+ + TP +I ++ G+ + Sbjct: 331 GNLGMKAFTTRILQRFGFRSVAVNNAAIAGGLIIACGLLTPATPLPVILGVIFCYGLSRS 390 Query: 366 LQFTCLNSIAYADIDNRDAGRVTSLASVIQQLSLGLGVTVAGIVLQLSNHLQGH------ 419 +QF+ L ++AYADI + ++L SV QQ+++G+G+ + L+++ L+G Sbjct: 391 MQFSTLATLAYADISDARKSSASTLWSVAQQMTIGMGIAFGALALRIAAALRGEAIDASG 450 Query: 420 TDIVEADFWPAFLVIGLFSVASVPISGKLPHNAGLELSRG 459 DF AF + G+ ++ SV L +AG + G Sbjct: 451 AHFTLGDFQWAFAMAGVVTLISVIGYRGLARDAGSNIGGG 490 >gi|300312683|ref|YP_003776775.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 231 bits (589), Expect = 8e-62, Method: Compositional matrix adjust. Identities = 145/441 (32%), Positives = 241/441 (54%), Gaps = 7/441 (1%) Query: 10 IVGCALFMENMDATVLATSLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMADKLG 69 IV FM+ +D T++ T+LPA+AR L + + + + AY + P SGW+AD+ G Sbjct: 57 IVASGFFMQTLDTTIVNTALPAMARSLGEPVLNMHPVVVAYTLTMASLTPASGWLADRFG 116 Query: 70 ARKVFSAAMIVFMTGSILCAAAGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDKSELV 129 R+++ AA+++F GS+LCA A SL +AAR +QG+GG+M++PVGR+ + R+V ++ + Sbjct: 117 TRRMYFAAILLFSLGSLLCAMASSLPMLLAARVVQGLGGSMLLPVGRLAVLRAVPGAQYI 176 Query: 130 KAVSYLTLPALLGPVVGPLLGGFITTYYHWRLVFLLNIPFCFLGLYLAHRFIENFKEDAP 189 A++ +++ LGPVVGP+LGG++ W +FL+N+P LG+ F+ + Sbjct: 177 AALASISIAGQLGPVVGPVLGGWLVESLSWHWIFLINLPVGVLGMGAVLYFLPDDSLAGT 236 Query: 190 APLDVKGFVLTSVGASMIMLGLSLFGSPLVDGAT--PVAMCSIGAIALVMYYRHSTQIPY 247 D+ G VL S A M+ L+L P T +A+ + A+ ++ Y+RH++ P Sbjct: 237 PRFDLLGCVLLS--ACMVAFSLAL-DVPFDSHRTGWSIALFVVAALTVLGYWRHASVHPR 293 Query: 248 PLLDFRLLRIPTLHASVVGGSLFRIGLGAVPFLLPLALQEGLGMTAFQAGSITCASAFGS 307 PL L P + G + RIG AVPFLLPL LQ LG + AG + +A Sbjct: 294 PLFRLGLFGEPNFSIGLAGNLVCRIGSSAVPFLLPLLLQVRLGYSPLHAGMVLLPAAIAG 353 Query: 308 LFIRLLASRVLRRYGFRTVLMVNAVFAGIALAAYGIFSLHTPYLLIFAIVLMGGVFPALQ 367 + + +++R+G+ L+VN G A+ ++ + + P + + G ++Q Sbjct: 354 TIAKPWIAPLVKRFGYDRFLLVNTAIVGAAIMSFALITPDWPLTAAIVQLAIFGASNSMQ 413 Query: 368 FTCLNSIAYADIDNRDAGRVTSLASVIQQLSLGLGVTVAGIVLQLSNHLQGHTDIVEADF 427 F +NS+ +D RDAG SL S++Q L++GLGVT+ G ++ L G A F Sbjct: 414 FAAMNSVTLKGLDKRDAGSGNSLFSMVQMLAIGLGVTIGGTMVNLFKSEVGTA--TNASF 471 Query: 428 WPAFLVIGLFSVASVPISGKL 448 F ++G ++AS + K+ Sbjct: 472 QITFAIVGAITLASAWVFRKM 492 >gi|300312566|ref|YP_003776658.1| multidrug resistance protein protein [Herbaspirillum seropedicae SmR1] Length = 525 Score = 114 bits (284), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 81/295 (27%), Positives = 144/295 (48%), Gaps = 4/295 (1%) Query: 9 LIVGCALFMENMDATVLATSLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMADKL 68 +++ A F+ +D T+ S+ +A L +T+Y A + +P++GW+A++ Sbjct: 36 ILLAAANFIAVLDTTIANVSVSTIAGALGASTSQGTYVITSYAVAEAITVPLTGWLANRF 95 Query: 69 GARKVFSAAMIVFMTGSILCAAAGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDKSEL 128 G +VF +M++F S LC A SL +A R LQG+ G ++P+ + ++ R K + Sbjct: 96 GTVRVFILSMVMFGLFSALCGMANSLGMLIAFRVLQGLAGGPLMPLSQTLLLRIFPKEKA 155 Query: 129 VKAVSYLTLPALLGPVVGPLLGGFITTYYHWRLVFLLNIPFCFLGLYLAHRFIENFKED- 187 AV + L+ P+ GP+LGG + Y W +F +N+P + Y+ R ++ ++ Sbjct: 156 PAAVGLWAMTTLVAPIAGPILGGVLCDQYSWPYIFWINVPVALICGYIGWRMLKRYETKM 215 Query: 188 APAPLDVKGFVLTSVGASMIMLGLSLFGSPLVDGATPVAMCSIGAIA-LVMYYRHSTQIP 246 + P+D G VL V + + L L +T + + ++ A+ + + Sbjct: 216 SKLPIDRVGLVLMVVWVAALQLMLDEGKEKDWFASTEIVLLAVTAVVFFIAFLIWELTER 275 Query: 247 YPLLDFRLLRIPTLHASVVGGSL-FRIGLGAVPFLLPLALQEGLGMTAFQAGSIT 300 P++D R+ R ASV L F G+V L PL LQ +G TA +G I+ Sbjct: 276 NPIVDLRVFRHRGFTASVTTICLAFGAFFGSV-VLTPLWLQGYMGYTATWSGCIS 329 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.329 0.143 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,392,859 Number of extensions: 59631 Number of successful extensions: 369 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 354 Number of HSP's successfully gapped: 17 Length of query: 460 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 372 Effective length of database: 1,203,190 Effective search space: 447586680 Effective search space used: 447586680 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)