BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1565.p (452 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311018|ref|YP_003775110.1| CBS domains containing hemolysi... 831 0.0 gi|300309700|ref|YP_003773792.1| Mg2+/Co2+ transporter transmemb... 116 3e-27 gi|300310418|ref|YP_003774510.1| hemolysin protein [Herbaspirill... 96 4e-21 gi|300310054|ref|YP_003774146.1| Mg2+ and Co2+ transporter prote... 81 2e-16 >gi|300311018|ref|YP_003775110.1| CBS domains containing hemolysin protein [Herbaspirillum seropedicae SmR1] Length = 442 Score = 831 bits (2147), Expect = 0.0, Method: Compositional matrix adjust. Identities = 417/435 (95%), Positives = 425/435 (97%) Query: 4 MSIFTSLLLILLLVVVSAFLSCAEISIAASRKIRLEMMSKEGEANADRVLSLQAQPGNFF 63 MSIFTSLLLILLLVVVSAFLSCAEISIAASRKIRLEMMSKEG NADRVLSLQAQPGNFF Sbjct: 1 MSIFTSLLLILLLVVVSAFLSCAEISIAASRKIRLEMMSKEGVHNADRVLSLQAQPGNFF 60 Query: 64 TVVQIGLNAVAILGGVVGEPALTPHMREIISTFYTGPLLENASFAMSFLVVTAFFILFAD 123 TVVQIGLNAVAILGGVVGEPALTPHMREIIS+FY GP LENASFAMSFLVVTAFFILFAD Sbjct: 61 TVVQIGLNAVAILGGVVGEPALTPHMREIISSFYIGPWLENASFAMSFLVVTAFFILFAD 120 Query: 124 LMPKRLAMAAPERAAVVLVKPMLLLETLFKPLVWFFNSLSKWIFTLFGLPQVRQDVITPS 183 LMPKRLAM APERAAV LVKPMLLLETLFKPLVWFFNSLSKWIFTLFGLPQVRQ+VITP+ Sbjct: 121 LMPKRLAMVAPERAAVALVKPMLLLETLFKPLVWFFNSLSKWIFTLFGLPQVRQEVITPN 180 Query: 184 EIHAMVDAGAQAGVLLTQEHHLIENVFELESRYVPSAMTPRDSIVYFLLTDSEETIRLKI 243 EIHAMVDAGAQAGVLLTQEHHLIENVFELESRYVPSAMTPRDSIVYFLLTDSE TIR KI Sbjct: 181 EIHAMVDAGAQAGVLLTQEHHLIENVFELESRYVPSAMTPRDSIVYFLLTDSEATIRQKI 240 Query: 244 IDHPHSRFLVCDGHIDAVLGYIDSKDVLKRLLKGEQFSLADKGLVHPPLSIPDSLNLWEV 303 IDHPHSRFLVCDGHIDAVLGYIDSKDVLKRLLKGEQFSLADKGLVHPPLSIPDSLNLWEV Sbjct: 241 IDHPHSRFLVCDGHIDAVLGYIDSKDVLKRLLKGEQFSLADKGLVHPPLSIPDSLNLWEV 300 Query: 304 LERMKAMDEDLAIVVNEYALVVGIISITDVTGVLMGNLLAITEEEQQIVKRDANSWLMDG 363 LERMKAMDEDLAIVVNEYALVVGIIS TDVTGVLMGNLLAITEEEQQIV+RD NSWLMDG Sbjct: 301 LERMKAMDEDLAIVVNEYALVVGIISTTDVTGVLMGNLLAITEEEQQIVRRDENSWLMDG 360 Query: 364 LTPLNDVMSELEIEEYPNPVGYETLSGFIMYMLRKIPKKTDFVLFSDFKFEVVDIEGNRI 423 LTPLNDVMSEL+I+EYPNPVGYETLSGFIMYMLRKIPKKTDFVLF++FKFEVVDIEGNRI Sbjct: 361 LTPLNDVMSELDIDEYPNPVGYETLSGFIMYMLRKIPKKTDFVLFAEFKFEVVDIEGNRI 420 Query: 424 NQLLVTRFKQDKSEK 438 NQLLVTRFKQDKSEK Sbjct: 421 NQLLVTRFKQDKSEK 435 >gi|300309700|ref|YP_003773792.1| Mg2+/Co2+ transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 434 Score = 116 bits (291), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 104/434 (23%), Positives = 206/434 (47%), Gaps = 29/434 (6%) Query: 1 MNNMSIFTSLLLILLLVVVSAFLSCAEISIAASRKIRLEMMSKEGEANADRVLSLQAQPG 60 M + I+ +L + +L+++SAF S E ++ A+ + RL ++K+G A +L L + Sbjct: 1 METVPIWVQVLALFILILLSAFFSMTETALMAANRHRLRHLAKQGNRAAITILWLLDRTE 60 Query: 61 NFFTVVQIGLNAVAILGGVVGEPALTPHMREIISTFYTGPLLENASFAMSFLVVTAFFIL 120 ++V I A+ L AL + I+ F + A+ A V A I+ Sbjct: 61 RLLSLVLIANTAINALA-----TALVTALA--IAAFGNHQRVITAATA----CVAALLII 109 Query: 121 FADLMPKRLAMAAPERAAVV---LVKPMLLLETLFKPLVWFFNSLSKWIFTLFGL---PQ 174 FA+++PK + PER ++ ++KP++ + KPL+WF N I L + + Sbjct: 110 FAEIVPKVIGATYPERISLFTGFILKPLM---RVAKPLIWFVNLFVSAILRLLRIKTGAR 166 Query: 175 VRQDVITPSEIHAMVDAGAQAGVLLTQEHH-LIENVFELESRYVPSAMTPRDSIVYFLLT 233 + I+P E+ ++V G G + Q+H ++ N+F+LE V MTPR + L Sbjct: 167 AAEQRISPEELRSIVLEG---GSFIPQKHKSILLNLFDLEKISVEDVMTPRSQVEALNLA 223 Query: 234 DSEETIRLKIIDHPHSRFLVCDGHIDAVLGYIDSKDVLKRLLKGEQFSLAD-KGLVHPPL 292 + I ++ H++ V +G I+ ++G + + + + E+ S D + L+ P Sbjct: 224 APVDDILHQLATCYHNKLPVFEGEINHIVGILHVRKTVSLFRREEEISHDDIRELLSAPY 283 Query: 293 SIPDSLNLWEVLERMKAMDEDLAIVVNEYALVVGIISITDVTGVLMGNLLAIT--EEEQQ 350 +P +++ L+ + E L +VV+EY V G++++ D+ ++G + + Sbjct: 284 YVPVETDVFTQLQYFQENHERLGVVVDEYGEVQGLVTLEDIIEEMIGEFTTSSPGSSGEG 343 Query: 351 IVKRDANSWLMDGLTPLNDVMSELEIEEYPNPVGYETLSGFIMYMLRKIPKKTDFVLFSD 410 + L++G T L D+ L ++ + G +T++G ++ L+ IP+ + V Sbjct: 344 FGWDKDGACLLEGSTTLRDINKRLGLQLPLD--GPKTINGLLLEYLQDIPEASVAVKMDG 401 Query: 411 FKFEVVDIEGNRIN 424 E+V ++ I Sbjct: 402 CVIEIVQVQNQAIR 415 >gi|300310418|ref|YP_003774510.1| hemolysin protein [Herbaspirillum seropedicae SmR1] Length = 440 Score = 96.3 bits (238), Expect = 4e-21, Method: Compositional matrix adjust. Identities = 93/425 (21%), Positives = 185/425 (43%), Gaps = 16/425 (3%) Query: 10 LLLILLLVVVSAFLSCAEISIAASRKIRLEMMSKEGEANADRVLSL-QAQPGNFFTVVQI 68 +++ LV ++ F AE S+ R+ R+ ++K + R+L++ Q + + Q+ Sbjct: 7 IVVAFFLVALNGFFVAAEFSLVKLRQTRIRAIAKT-QGMRGRILAVVHNQLDAYLSACQL 65 Query: 69 GLNAVAILGGVVGEPALTPHMREIIS--TFYTGPLLENASFAMSFLVVTAFFILFADLMP 126 G+ ++ G +GEPA + + S L+ SF +F V++ I+ +L P Sbjct: 66 GITLASLGLGWIGEPAFARILEPLFSLAGVTNQELIHGVSFVFAFFVISFLHIVAGELAP 125 Query: 127 KRLAMAAPERAAVVLVKPMLLLETLFKPLVWFFNSLSKWIFTLFGL--PQVRQDVITPSE 184 K +A+ +PE+ + P+ PL+W N+ S W+ + GL + E Sbjct: 126 KSMAIRSPEKLGLWCAMPLYGFYWGMYPLIWVLNASSNWLLRVAGLGAGHGHDAHYSSDE 185 Query: 185 IHAMVDAGAQA---GVLLTQEHHLIENVFELESRYVPSAMTPRDSIVYFLLTDSEETIRL 241 + ++ AG ++ G E +++ V M P IV S E Sbjct: 186 LKLILRAGNKSGKNGKFTRDEWNVLTQSLNFAELDVADIMRPASEIVALGDDKSLEENLD 245 Query: 242 KIIDHPHSRFLVCDGHIDAVLGYIDSKDVLKRLLKGEQFSLAD-KGLVHPPLSIPDSLNL 300 I + +SR+ D VLG + KDV L + + ++A+ K + P I +L Sbjct: 246 IIYRNRYSRYPYYDAERQQVLGLVHLKDVF--LAQQDGRAIANLKDYLRPVQYISPALPA 303 Query: 301 WEVLERMKAMDEDLAIVVNEYALVVGIISITDVTGVLMGNLL-AITEEEQQIVKRDANSW 359 ++L R + A++ + G I++ ++ +L+G + + ++D + Sbjct: 304 LDLLRRFRTGSPHFAVIGKKGQPPAGFITLDNMLSLLVGEIRDEFRHNTGEWTRQDDGTL 363 Query: 360 LMDGLTPLNDVMSELEIE-EYPNPVGYETLSGFIMYMLRKIPKKTDFVLFSDFKFEVVDI 418 L G P+ + + L I+ EY + + +++ G +M L +PK+ + F F + + Sbjct: 364 LGKGSLPIVTLENMLGIDIEYDDSI--DSVGGLVMEKLGDLPKEGQKIDFPAFDVVIKRM 421 Query: 419 EGNRI 423 G +I Sbjct: 422 SGPKI 426 >gi|300310054|ref|YP_003774146.1| Mg2+ and Co2+ transporter protein [Herbaspirillum seropedicae SmR1] Length = 292 Score = 80.9 bits (198), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 67/241 (27%), Positives = 116/241 (48%), Gaps = 26/241 (10%) Query: 205 LIENVFELESRYVPSAMTPRDSIVYFLLTDSEETIRLKIIDHPHSRFLVCDGHIDAVLGY 264 +IE VF++ V M PR + ++ ET +++ HSRF DG D V+G Sbjct: 56 MIEGVFQVSDLAVRDIMVPRSQMDTIDISKPIETWMPEVLATSHSRFPAVDGERDKVVGI 115 Query: 265 IDSKDVLKRLLKGEQFSLADKGLVHPPLSIPDSLNLWEVLERMKAMDEDLAIVVNEYALV 324 + +KD+L R E F + D ++ P + IP+S L +L +A +A+VV+EY V Sbjct: 116 LLAKDLL-RYYAEESFDVRD--MLRPAIFIPESKRLNVLLRDFRANRNHMAVVVDEYGGV 172 Query: 325 VGIISITDVTGVLMGNL---LAITEEEQQIVK-RD---ANSWLMDGLTPLNDVMSELEIE 377 G+I+I DV ++G++ E E ++ +D W + LT EIE Sbjct: 173 AGLITIEDVLEQIVGDIEDEYDFDEAEDNVLALKDGPHGARWRVKALT---------EIE 223 Query: 378 EYPNPVGY-------ETLSGFIMYMLRKIPKKTDFVLFSDFKFEVVDIEGNRINQLLVTR 430 ++ VG +T+ G + + ++P+K D FEV+ + +++ LLV++ Sbjct: 224 QFNEEVGSRFSDDDADTIGGLVANHVGRVPRKGDEFDIDGLHFEVLRADARQVHVLLVSQ 283 Query: 431 F 431 Sbjct: 284 L 284 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.137 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,224,463 Number of extensions: 50607 Number of successful extensions: 136 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 129 Number of HSP's successfully gapped: 4 Length of query: 452 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 364 Effective length of database: 1,203,190 Effective search space: 437961160 Effective search space used: 437961160 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)