BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1557.p (775 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311015|ref|YP_003775107.1| anaerobic (selenocysteine-conta... 1578 0.0 gi|300310774|ref|YP_003774866.1| anaerobic formate dehydrogenase... 718 0.0 gi|300310808|ref|YP_003774900.1| NAD-dependent formate dehydroge... 144 4e-35 gi|300312208|ref|YP_003776300.1| assimilatory nitrate reductase ... 102 1e-22 gi|300313158|ref|YP_003777250.1| anaerobic dehydrogenase family ... 62 2e-10 >gi|300311015|ref|YP_003775107.1| anaerobic (selenocysteine-containing) formate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 775 Score = 1578 bits (4087), Expect = 0.0, Method: Compositional matrix adjust. Identities = 751/775 (96%), Positives = 765/775 (98%) Query: 1 MPSKPRIEQYNQPAGGWGALKYVALNLVKEHVADGKGLRTLLSQNQPDGFDCPGCAWPDR 60 MPSKPRIEQYNQPAGGWGALKYVALNLVKEHVADG+GLRTLLSQNQPDGFDCPGCAWPDR Sbjct: 1 MPSKPRIEQYNQPAGGWGALKYVALNLVKEHVADGRGLRTLLSQNQPDGFDCPGCAWPDR 60 Query: 61 EHTSTFEFCENGVKAVAAEATKKRVTPEFFEKHTVTELMQQSDYELEEHGRLTHPMVYDA 120 EHTSTFEFCENGVKAVAAEATKKRVTPEFF +HTVTELMQQSD+ELEEHGRLTHPMVYDA Sbjct: 61 EHTSTFEFCENGVKAVAAEATKKRVTPEFFAQHTVTELMQQSDFELEEHGRLTHPMVYDA 120 Query: 121 ATDKYRKIEWSAAFELMAKHLNALPDPDMADFYVSGRASNEAAFLFQLFVRQYGTNNFPD 180 ATDKY+KIEW+ AF LMAKHLNALPDPDMADFYVSGRASNEAAFLFQLFVRQYGTNNFPD Sbjct: 121 ATDKYKKIEWADAFALMAKHLNALPDPDMADFYVSGRASNEAAFLFQLFVRQYGTNNFPD 180 Query: 181 CSNMCHEPTSVGLPGTVGIGKGTVLLEDFDHCDTLLLFGQNPATNHPRMMGELRHASKRG 240 CSNMCHEPTSVGLPGTVGIGKGTVLLEDFDHCDTLLLFGQNPATNHPRMMGELRHASKRG Sbjct: 181 CSNMCHEPTSVGLPGTVGIGKGTVLLEDFDHCDTLLLFGQNPATNHPRMMGELRHASKRG 240 Query: 241 ATIVAINPLKERGLERFADPQSKVEMLTMGSTRISSMFIHPKLGGDLALIKGVIKRTIEL 300 ATIVAINPLKERGLERFADPQSKVEMLTMGSTRISSMFIHP LGGDLALIKGVIKRTIEL Sbjct: 241 ATIVAINPLKERGLERFADPQSKVEMLTMGSTRISSMFIHPTLGGDLALIKGVIKRTIEL 300 Query: 301 DDVAQAAGTERVIDVAFIEQHTAGFEEFAAQARAESWNDIVEESGVSREDIEKLAQVYVK 360 DD A+AAG ERVIDV FIE+HTAGFEEFAAQARAESW+DIV ESGVSREDIEKLAQVYVK Sbjct: 301 DDAARAAGAERVIDVKFIEEHTAGFEEFAAQARAESWDDIVAESGVSREDIEKLAQVYVK 360 Query: 361 GKAVIATWGMGLTQHKYAVATIQLLSNMMMLRGNIGRQGAGLCPVRGHSNVQGDRTVGID 420 GKAVIATWGMGLTQHK+AVATIQLLSNMMMLRGNIGRQGAGLCPVRGHSNVQGDRTVGID Sbjct: 361 GKAVIATWGMGLTQHKHAVATIQLLSNMMMLRGNIGRQGAGLCPVRGHSNVQGDRTVGID 420 Query: 421 EKPTPAFLDRLEQVFGFKAPRHHGNDTVGSVMAMLEGRTKVFIGLGGNFAMATPDTPRTF 480 EKPTPAFLDRLEQVFGFKAPRHHGNDTVGSVMAMLEGRTKVFIGLGGNFAMATPDTPRTF Sbjct: 421 EKPTPAFLDRLEQVFGFKAPRHHGNDTVGSVMAMLEGRTKVFIGLGGNFAMATPDTPRTF 480 Query: 481 EALRSCNLTVHITTKLNRSHLVHGKDALILPTMGRTEIDVQKSGPQGVTVEDSMSMVHVS 540 EA+RSCNLTVHITTKLNRSHLVHGKDALILPTMGRTEID+QKSGPQGVTVEDSMSMVHVS Sbjct: 481 EAMRSCNLTVHITTKLNRSHLVHGKDALILPTMGRTEIDMQKSGPQGVTVEDSMSMVHVS 540 Query: 541 YGINKPASEHLMSETAIVAHLAEATMKSRNNGPKIDWLWYAEDYSRIRDAIEKVYDAFKG 600 YGINKPASEHLMSETAIVAHLAEATMKSRNNGPKIDWLWYAEDYSRIRDAIE+VYDAFKG Sbjct: 541 YGINKPASEHLMSETAIVAHLAEATMKSRNNGPKIDWLWYAEDYSRIRDAIEQVYDAFKG 600 Query: 601 YNERIANPGGFHLGVASRDRVWKTGSGKANFIVHEIPKDTPIHRARAIHGEKLLTLMTTR 660 YNERIANPGGFHLGVASRDRVWKT SGKANFIVH+IPKDTPIHRARAIHGEKLLTLMTTR Sbjct: 601 YNERIANPGGFHLGVASRDRVWKTASGKANFIVHDIPKDTPIHRARAIHGEKLLTLMTTR 660 Query: 661 SHDQYNTTIYGMDDRYRGVFGQRRVVFIHPQDLQMLGLKDGEWVDITSVWDDGVERRAEG 720 SHDQYNTTIYGMDDRYRGVFGQRRVVFI+P DLQMLGLKDGEWVDITSVWDDGVERRAEG Sbjct: 661 SHDQYNTTIYGMDDRYRGVFGQRRVVFINPADLQMLGLKDGEWVDITSVWDDGVERRAEG 720 Query: 721 FLLVEYEIPRGCIGSYYPETNPLVPLESFADKARTPTSKSIPVLLSRSAVQRSAA 775 FLLVEYEIPRGCIGSYYPETNPLVPLESFADKARTPTSKSIPVLLSRSAVQRSAA Sbjct: 721 FLLVEYEIPRGCIGSYYPETNPLVPLESFADKARTPTSKSIPVLLSRSAVQRSAA 775 >gi|300310774|ref|YP_003774866.1| anaerobic formate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 790 Score = 718 bits (1854), Expect = 0.0, Method: Compositional matrix adjust. Identities = 361/773 (46%), Positives = 490/773 (63%), Gaps = 23/773 (2%) Query: 1 MPSKPRIEQYNQPAGGWGALKYVALNLVKEHVADGKGLRTLLSQNQPDGFDCPGCAWPDR 60 +P ++YN AGGWG++K + L +EHV G R L+ QN+P+GF C C+W Sbjct: 23 IPEDATFDEYNNAAGGWGSVKALGSILQQEHVI-ASGTRILMHQNKPEGFACVSCSWAKP 81 Query: 61 EHTSTFEFCENGVKAVAAEATKKRVTPEFFEKHTVTELMQQSDYELEEHGRLTHPMVYDA 120 FEFCENG KA A E T+KR T FF +HTVTE+ +D ELE GR+T PM YDA Sbjct: 82 ADPHLFEFCENGAKATAWEITRKRATAAFFAQHTVTEMEAWNDLELESSGRVTEPMKYDA 141 Query: 121 ATDKYRKIEWSAAFELMAKHLNALPDPDMADFYVSGRASNEAAFLFQLFVRQYGTNNFPD 180 A+D+Y + WS AF+ +A L A+ DP FY SGRAS E ++L+QL R YGTNN PD Sbjct: 142 ASDRYLPVSWSEAFDDIAAQLRAM-DPKKVVFYASGRASLETSYLYQLMARMYGTNNLPD 200 Query: 181 CSNMCHEPTSVGLPGTVGIGKGTVLLEDFDHCDTLLLFGQNPATNHPRMMGELRHASKRG 240 SNMCHE TSV LP T+G+ GTV L+DF D +L FG N TN PRM+ L KRG Sbjct: 201 SSNMCHESTSVALPQTIGVPVGTVTLDDFGQTDCILFFGHNTGTNAPRMLHPLEEVRKRG 260 Query: 241 ATIVAINPLKERGLERFADPQSKVEMLTMGSTRISSMFIHPKLGGDLALIKGVIKRTIEL 300 ++ NPL+ERGLERF +PQS EM+T T IS+ ++ K+GGD A G+ KR +E Sbjct: 261 VPVITFNPLRERGLERFVNPQSPKEMVTPAHTDISTQYLQIKIGGDSAAAIGMAKRILER 320 Query: 301 DDVAQAAGTERVIDVAFIEQHTAGFEEFAAQARAESWNDIVEESGVSREDIEKLAQVYVK 360 DD A+ G R++D+AFIE+HT+GFE+FA RA W+++ +GVSR ++E A Y Sbjct: 321 DDKARQEGLPRLLDLAFIEEHTSGFEQFADAVRAAPWDELERHAGVSRAELELAADTYAG 380 Query: 361 GKAVIATWGMGLTQHKYAVATIQLLSNMMMLRGNIGRQGAGLCPVRGHSNVQGDRTVGID 420 + +GMG+TQH+ AV TI +L+N++++RGNIG+ GAG+CP+RGHSNVQG RTVGI Sbjct: 381 AGRAMLMYGMGVTQHREAVRTIHMLTNLLLMRGNIGKPGAGICPIRGHSNVQGQRTVGIT 440 Query: 421 EKPTPAFLDRLEQVFGFKAPRHHGNDTVGSVMAMLEGRTKVFIGLGGNFAMATPDTPRTF 480 EKP D+L ++ F+ P G +TV + + +G F LGGNF A PD Sbjct: 441 EKPELVPNDKLRALYHFEPPMEKGLNTVEACEKIRDGELSAFFMLGGNFVRAIPDHGVME 500 Query: 481 EALRSCNLTVHITTKLNRSHLVHGKDALILPTMGRTEIDVQKSGPQGVTVEDSMSMVHVS 540 A R LTV + T NRS ++HG+ + +LP +GR EID Q+ G Q V+VEDS +H S Sbjct: 501 AAWRKLPLTVQVVTHFNRSCVIHGQTSYVLPCLGRIEIDRQRGGEQAVSVEDSTGCMHGS 560 Query: 541 YGINKPASEHLMSETAIVAHLAEATMKSRNNGPKIDWLWYAEDYSRIRDAIEKVY-DAFK 599 G +PAS HL+SE AIVA LA+ + P++DW + DY+RIRDAIE Y D F+ Sbjct: 561 RGRAEPASPHLLSEPAIVAELAKRLLPF---NPQVDWDGWVGDYARIRDAIEATYPDIFR 617 Query: 600 GYNERIANPGGFHLGVASRDRVWKTGSGKANFIV-------HEIPKDTPIHRARAIHGEK 652 +N R+ NPGGF +A+R+R WKT +G+ANF+ ++P D P + Sbjct: 618 DFNARMWNPGGFARPLAARERQWKTPNGRANFMTPDGFDPDPDMPGDAP----------E 667 Query: 653 LLTLMTTRSHDQYNTTIYGMDDRYRGVFGQRRVVFIHPQDLQMLGLKDGEWVDITSVWDD 712 +L LMTTR Q+NTT+Y +DDR+RGV+G RRV+ ++ +DLQ GL G++V +V D Sbjct: 668 VLRLMTTRGDSQFNTTVYSLDDRFRGVWGTRRVLLMNLRDLQQHGLAAGDFVCAETVSAD 727 Query: 713 GVERRAEGFLLVEYEIPRGCIGSYYPETNPLVPLESFADKARTPTSKSIPVLL 765 GVERR + ++IP GC+ Y+PE N L+PL A ++ P +KS+P+ L Sbjct: 728 GVERRVPHLRVEPFDIPVGCVMGYFPELNRLIPLFHHARGSKVPAAKSVPIRL 780 >gi|300310808|ref|YP_003774900.1| NAD-dependent formate dehydrogenase subunit alpha [Herbaspirillum seropedicae SmR1] Length = 957 Score = 144 bits (362), Expect = 4e-35, Method: Compositional matrix adjust. Identities = 163/649 (25%), Positives = 249/649 (38%), Gaps = 119/649 (18%) Query: 111 RLTHPMVYDAATDKYRKIEWSAAFELMA---KHLNALPDPDMADFYVSGRASNEAAFLFQ 167 R+ +PM+ + D +R++ W A A + + A D S R +NE +L Q Sbjct: 298 RILNPMIRKSINDPWREVSWEEALSYAASEFRRIQAKHGKDSIGGITSSRCTNEETYLVQ 357 Query: 168 LFVRQ-YGTNNFPDCSNMCHEPTSVGLPGTVGIGKGTVLLEDFDHCDTLLLFGQNPATNH 226 VR +G NN C+ +CH PT GL T+G GT E D +L+ G NPA H Sbjct: 358 KLVRAAFGNNNVDTCARVCHSPTGYGLKQTLGESAGTQTFESVMKSDVILIMGANPAAGH 417 Query: 227 PRMMGELRHASKRGATIVAINPLKERGLERFADPQSKVEMLTMGSTRISSMFIHPKLGGD 286 P +++ ++GA ++ I+P R + M P + D Sbjct: 418 PVFASQIKRRVRQGAKLIVIDP------------------------RTTEMVKSPHIQAD 453 Query: 287 LALIKGVIKRTIELDDVAQAAGTERVIDVAFIE-----QHTAGFEEFAAQARAESWNDIV 341 L T + +A +E + D A+I+ Q ++EF + S + Sbjct: 454 YHLKLRPGTNTAIITALAHVILSEGLQDEAYIQERCDLQSYQDWKEFVLR-EDNSPEAMA 512 Query: 342 EESGVSREDIEKLAQVYVKGKAVIATWGMGLTQHKYAVATIQLLSNMMMLRGNIGRQGAG 401 +GV E I A++Y G +G+G+T+H T+ ++N+ M GN+GR+G G Sbjct: 513 AITGVPAEIIRGAARLYATGGNAAIYYGLGVTEHAQGSTTVMGIANLAMCTGNVGREGVG 572 Query: 402 LCPVRGHSNVQGDRTVGIDEKPTPAF--------LDRLEQVFGFKAPRHHGNDTVGSVMA 453 + P+RG +NVQG +G P + ++ EQ +G G A Sbjct: 573 VNPLRGQNNVQGSCDMGSFPHELPGYRHISDSTVRNQFEQAWGVTLNPEPGLRIPNMFDA 632 Query: 454 MLEGRTKVFIGLGGNFAMATPDTPRTFEALRSCNLTVHITTKLNRS-HLVHGKDALILPT 512 L+G K G + + P+T AL++ V LN + H + LP Sbjct: 633 ALDGSFKGLYCEGEDIVQSDPNTQHVAAALQAMECIVVQDLFLNETAKYAH----VFLPG 688 Query: 513 MGRTEIDVQKSGPQGVTVEDSMSMVHVSYGINKPASEHLMSETAIVAHLAEATMKSRNNG 572 E D G + V + A + S+ + LAEA Sbjct: 689 SSFLEKD-------GTFTNAERRISRVRKVMPPKAGK---SDWEVTVALAEA-------- 730 Query: 573 PKIDWLWYAEDY---SRIRDAIEKVYDAFKGYNERIANPGGFHLGVASRDRVWKTGSGKA 629 L Y Y S I D I + +F G S D++ K GS Sbjct: 731 -----LGYPMPYNHPSEIMDEIAALTPSFHG---------------VSYDKLEKLGS--I 768 Query: 630 NFIVHE-IPKDTPI-HRARAIHG-------------EKL-----LTLMTTRSHDQYNTTI 669 + +E P+ TPI H I G EK+ L L T R QYN Sbjct: 769 QWPCNESAPEGTPIMHVGSFIRGKGKFINTQYFATDEKVTQRYPLILTTGRILSQYNVGA 828 Query: 670 YGMDDRYRGVFGQRRVVFIHPQDLQMLGLKDGEWVDITSVWDDGVERRA 718 + R+ IHP D + G+++G+WV GVE RA Sbjct: 829 QTRRTENSQWHSEDRLE-IHPHDAEDRGIREGDWV--------GVESRA 868 >gi|300312208|ref|YP_003776300.1| assimilatory nitrate reductase large subunit [Herbaspirillum seropedicae SmR1] Length = 922 Score = 102 bits (254), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 146/662 (22%), Positives = 239/662 (36%), Gaps = 107/662 (16%) Query: 110 GRLTHPMVYDAATDKYRKIEWSAAFELMAKHLNAL---PDPDMADFYVSGRASNEAAFLF 166 GRL HP + DA + +++ W A + +A +A+ PD YVSG+ E ++ Sbjct: 68 GRLLHPQLRDA-QGQLQQLAWDQALDHVASKFSAIIAEHGPDAVALYVSGQLLTEDYYIA 126 Query: 167 QLFVRQY-GTNNFPDCSNMCHEPTSVGLPGTVGIGKGTVLLEDFDHCDTLLLFGQNPATN 225 ++ Y G+ N S +C G G V ED + D ++L G N A Sbjct: 127 NKLMKGYIGSANIDTNSRLCMSSAVAGHKRAFGEDLVPVCYEDLELADLVVLVGSNAAWC 186 Query: 226 HPRMMGEL-RHASKRGAT-IVAINPLKERGLERFADPQSKVEMLTMGSTRISSMFIHPKL 283 HP + + R R A +V I+P + E ++ + + K Sbjct: 187 HPILFQRIARLKETRPALRLVVIDPRRTATCE------------------LADLHLPIKP 228 Query: 284 GGDLALIKGVIKRTIELDDVAQAAGTERVIDVAFIEQHTAGFEEFAAQARAESWN--DIV 341 G D+ L G++ + D AFIE HT GF+E A A A+ + + Sbjct: 229 GTDVWLFNGLMSYLAH----------RGLRDNAFIEAHTKGFDEALAVADADCADPATVA 278 Query: 342 EESGVSREDIEKLAQVYVKGKAVIATWGMGLTQHKYAVATIQLLSNMMMLRGNIGRQGAG 401 + +D+ + + + V+ + MG+ Q + + N + G IG+ G G Sbjct: 279 RLCKIDLKDLLAFYEAFANTEKVVTGFSMGVNQSSAGTDKVNSIINCHLATGRIGKPGMG 338 Query: 402 LCPVRGHSNVQGDRTVGIDEKPTPAFLD-------RLEQVFGFKAPR---HHGNDTVGSV 451 + G N G R VG A +D ++ Q F +++PR G V Sbjct: 339 PFSITGQPNAMGGREVGGLANMLAAHMDLDQPLHRQIVQDF-WQSPRIADKPGLKAVDMF 397 Query: 452 MAMLEGRTKVFIGLGGNFAMATPDTPRTFEALRSCNLTVH--ITTKLNRSHLVHGKDALI 509 A+ EGR K + N ++ PD AL+ C L V + T+ + + L H ++ Sbjct: 398 RAIEEGRIKAVWIMATNPMVSLPDADLVQRALQKCELVVSSDVITQTDTNALAH----VL 453 Query: 510 LPTMGRTEIDVQKSGPQGVTVEDSMSMVHVSYGINKPASEHLMSETAIVAHLAE------ 563 LP + E D G + ++ P + I+ +A Sbjct: 454 LPALAWGEKD-------GTVTNSERRISRQRAFLSAPGEAR--GDWRIICDVARRMGFDG 504 Query: 564 -------------ATMKSRNNGPKIDWLWYAEDYSRIRDAI---------EKVYDAFKGY 601 A + + NG D SR+R YDA Sbjct: 505 FDFAGPHEIFDEHARLSAHRNGEA--------DASRVRRVFNLAGLTGMDRAAYDALAPI 556 Query: 602 N---ERIANPGGFHLGVASRDRVWKTGSGKANFIVHEIPKDTPIHRARAIHGEKLLTLMT 658 +R+++ D + G+A F+ P A A + L L T Sbjct: 557 QWPVQRLSDGSVSGTARVLEDHRFSHADGRARFV-----PTAPRSAAHAACEDYPLILNT 611 Query: 659 TRSHDQYNTTIYGMDDRYRGVFGQRRVVFIHPQDLQMLGLKDGEWVDITSVWDDGVERRA 718 R D ++T + IHPQD + G+++G I+S W V R Sbjct: 612 GRVRDHWHTMTRTGRSATLASHIPESFIDIHPQDALLYGVREGGLARISSRWGAMVARVQ 671 Query: 719 EG 720 G Sbjct: 672 HG 673 >gi|300313158|ref|YP_003777250.1| anaerobic dehydrogenase family protein [Herbaspirillum seropedicae SmR1] Length = 701 Score = 61.6 bits (148), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 66/268 (24%), Positives = 108/268 (40%), Gaps = 43/268 (16%) Query: 111 RLTHPM--VYDAATDKYRKIEWSAAFELMAKHLN--ALPDPD--MADFYVSGRASNEAAF 164 RL HPM V K+ +I W A +A+ L AL DP + Y + Sbjct: 61 RLLHPMRRVGKKGEGKFERISWDEAIATIAQRLGQIALRDPQAILPYSYAGTMGQVQGES 120 Query: 165 LFQLFVRQYGTNNFPDCSNMCHEPTSVGLPGTVGIGKGTVLLEDFDHCDTLLLFGQNPAT 224 + F + G + +C G T+G G L E DH +L++G NP Sbjct: 121 MAGRFFHRLGASQLE--RTICASAGGAGYRYTIGARVGADL-EQVDHAKLILIWGGNPIA 177 Query: 225 NHPRMMGELRHASKRGATIVAINPLKERGLERFADPQSKVEMLTMGSTRISSMFIHPKLG 284 ++ ++ A + GA ++AI+P + E+ + +L G Sbjct: 178 SNLHFWTRVQTAKRNGARLIAIDPYRSLTAEKC---HQHIALLP---------------G 219 Query: 285 GDLALIKGVIKRTIELDDVAQAAGTERVIDVAFIEQHTAGFEEFAAQARAESWND--IVE 342 D AL G++ I +E ++D +I +HT GFE+ + R W E Sbjct: 220 TDAALALGMMHVLI----------SEDLLDHDYIARHTLGFEQL--RERVAEWTPARTAE 267 Query: 343 ESGVSREDIEKLAQVYVKGKAVIATWGM 370 G+S E++ LA+ Y G+ +A G+ Sbjct: 268 VCGISAEEVLNLAREY--GRVALAGEGV 293 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,530,504 Number of extensions: 111036 Number of successful extensions: 254 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 244 Number of HSP's successfully gapped: 5 Length of query: 775 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 682 Effective length of database: 1,179,515 Effective search space: 804429230 Effective search space used: 804429230 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)