BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1554.p (545 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311012|ref|YP_003775104.1| methyl-accepting chemotaxis tra... 823 0.0 gi|300310789|ref|YP_003774881.1| methyl-accepting chemotaxis tra... 311 8e-86 gi|300310620|ref|YP_003774712.1| methyl-accepting chemotaxis tra... 308 1e-84 gi|300312783|ref|YP_003776875.1| methyl-accepting chemotaxis tra... 305 8e-84 gi|300313891|ref|YP_003777983.1| methyl-accepting chemotaxis I p... 299 4e-82 >gi|300311012|ref|YP_003775104.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 550 Score = 823 bits (2127), Expect = 0.0, Method: Compositional matrix adjust. Identities = 443/526 (84%), Positives = 476/526 (90%), Gaps = 1/526 (0%) Query: 1 MSFLQNLRVSARLALLSALFLVAMLVMVSWSIFSLHSISSQERQMYQERIAPMQKLNTAA 60 MSFLQ LRVSARLALLSALFL AML MVSW+I SL SIS+QERQMY +RI PMQKLNTAA Sbjct: 1 MSFLQKLRVSARLALLSALFLAAMLAMVSWNIVSLRSISAQERQMYHDRIEPMQKLNTAA 60 Query: 61 LAMAIHFRRGYSYIYPNANDPKSRVGTRALNQKAEAQIQDAFDYLQGAEKSPQLEPLLAA 120 LAMAIHFRRGYSYIYPNA DPKSR+GTRALNQKAEAQIQ+AFDYLQGAEK+ QL PL++A Sbjct: 61 LAMAIHFRRGYSYIYPNAGDPKSRIGTRALNQKAEAQIQEAFDYLQGAEKTAQLAPLVSA 120 Query: 121 LNQAWPAYRESFQRLMDKADQDDVPGALAELQKTTDPAHVKLRDVFLQIVKQYDQAVQDH 180 LNQAWPAY+ESFQRLMDKADQDD GALAELQKTTDPAHVKLRDVFLQ+VK YDQ+VQDH Sbjct: 121 LNQAWPAYKESFQRLMDKADQDDQAGALAELQKTTDPAHVKLRDVFLQVVKLYDQSVQDH 180 Query: 181 ISRSAESVDRTAWYLVALLAAMVVVGVAFSVVIQRSITRQLGGEPLYAVAIAQAIADGDL 240 ++ SAE VD TAW LVALLA V +GVAFSVVIQRSITRQLGGEP YAV IAQAIADGDL Sbjct: 181 MNVSAEGVDSTAWRLVALLALTVALGVAFSVVIQRSITRQLGGEPDYAVDIAQAIADGDL 240 Query: 241 ASRVKLRQGDQGSVLTAMESMRLRLSHLVQEVYKSCHAIDTGASEIADGNLNLSRRTEEQ 300 A++V+LR GDQGSVL AME+MR RL+HLVQEVYKSCHAIDTGASEIADGNLNLS+RTEEQ Sbjct: 241 ATQVRLRAGDQGSVLAAMETMRARLAHLVQEVYKSCHAIDTGASEIADGNLNLSQRTEEQ 300 Query: 301 ASNLEQTAASMEQLTGSVKQNAQTVGEAVKRAGDASQEASQGGDAVAGVVDLMGDIASSS 360 ASNLE+TAASME+LT +VK NAQTV EAVK AGDASQ A GG AV+GVVDLMGDIASSS Sbjct: 301 ASNLEETAASMEELTSTVKHNAQTVAEAVKLAGDASQAAGHGGQAVSGVVDLMGDIASSS 360 Query: 361 ARIKEIIAVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRTLAHRSAQAAREIK 420 ARIKEII VID IAFQTNILALNAAVEAARAGEQGRGFAVVASEVRTLAHRSAQAAREIK Sbjct: 361 ARIKEIIVVIDAIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRTLAHRSAQAAREIK 420 Query: 421 TLIEDSVDKVDSGYAKVQEASQAISGVVDRVRQVATLVTEIGVSTQEQTQGIAQVGTAVE 480 TLIEDSV KVD+GY+KVQEA+ AI VV +VR V+ LV EIGVSTQEQTQGI+QVGTAV+ Sbjct: 421 TLIEDSVSKVDAGYSKVQEAADAIGEVVTQVRHVSGLVNEIGVSTQEQTQGISQVGTAVQ 480 Query: 481 QLDNSTQQNAALVEQSAAAAESLSAQASKLLELMQVFKLPVGAHEQ 526 QLD+ TQQNAALVEQSAAAAESLSAQASKLL+LM FKL G EQ Sbjct: 481 QLDDVTQQNAALVEQSAAAAESLSAQASKLLDLMGAFKLA-GREEQ 525 >gi|300310789|ref|YP_003774881.1| methyl-accepting chemotaxis transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 542 Score = 311 bits (797), Expect = 8e-86, Method: Compositional matrix adjust. Identities = 190/422 (45%), Positives = 274/422 (64%), Gaps = 24/422 (5%) Query: 102 FDYLQGAEKSPQLEPLLAALNQAWPAYRESFQRLMDKADQDDVPGALAELQKTTDPAHVK 161 FD + A+ + EPL+ NQA + A QD ++ L + P K Sbjct: 116 FDLI--AQNAADTEPLM---NQA-----------VTLAQQDKSAELISLLTQKIRPVQAK 159 Query: 162 LRDVFLQIVKQYDQAVQDHISRSAESVDR----TAWYLVALLAAMVVVGVAFSVVIQRSI 217 D L + +++ + + S +A++ R T W LVA V++ VA + +I RS+ Sbjct: 160 WLD-NLDELGRFEDELNNQASAAADTTYRSILTTTWTLVA---GSVLLAVAAAFLIVRSV 215 Query: 218 TRQLGGEPLYAVAIAQAIADGDLASRVKLRQGDQGSVLTAMESMRLRLSHLVQEVYKSCH 277 QLGGEP A +A IA G+LA + L +GDQ S++ ++E+MR +L+ +V + S Sbjct: 216 LHQLGGEPAQAQELAAQIAQGNLAVSIPLAKGDQHSLMASLENMRAQLTRIVDGIKHSAE 275 Query: 278 AIDTGASEIADGNLNLSRRTEEQASNLEQTAASMEQLTGSVKQNAQTVGEAVKRAGDASQ 337 +I T AS+IA GNL+LSRRTEEQA++LE+TA+SME++T +V+QN Q G+ + A AS Sbjct: 276 SISTAASQIAVGNLDLSRRTEEQAASLEETASSMEEITSTVEQNTQNAGQGHQLARSASL 335 Query: 338 EASQGGDAVAGVVDLMGDIASSSARIKEIIAVIDGIAFQTNILALNAAVEAARAGEQGRG 397 A +GG+ V VV M DI+ S+ +I EIIA ++GIAFQTNILALNAAVEAARAGEQGRG Sbjct: 336 HAQKGGEVVERVVGTMKDISQSAYKITEIIATVEGIAFQTNILALNAAVEAARAGEQGRG 395 Query: 398 FAVVASEVRTLAHRSAQAAREIKTLIEDSVDKVDSGYAKVQEASQAISGVVDRVRQVATL 457 FAVVA+EVR+LA RSA +A+EI+ LIE SV V++G V EA + VV ++QV L Sbjct: 396 FAVVATEVRSLAQRSASSAKEIRGLIESSVQHVEAGQKLVDEAGLTMDEVVRSIQQVNDL 455 Query: 458 VTEIGVSTQEQTQGIAQVGTAVEQLDNSTQQNAALVEQSAAAAESLSAQASKLLELMQVF 517 ++EI ++ EQ+ GI+Q+ A+ Q+D TQQNAALVE+SA+AA+S++ QA+ L + + VF Sbjct: 456 MSEITAASNEQSSGISQINIAISQMDEVTQQNAALVEESASAAQSMAQQANSLRDAVAVF 515 Query: 518 KL 519 ++ Sbjct: 516 RI 517 >gi|300310620|ref|YP_003774712.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 573 Score = 308 bits (788), Expect = 1e-84, Method: Compositional matrix adjust. Identities = 208/531 (39%), Positives = 316/531 (59%), Gaps = 23/531 (4%) Query: 1 MSFLQNLRVSARLALLSALFLVAML--VMVSWSIFSLHSISSQERQMYQERIAPMQKLNT 58 M++ NL+VS +L + +VA++ + ++ I I+ +MY+ ++ ++ Sbjct: 1 MNWFTNLKVSHKL--IGGFMIVALIGATIGTFGIIKAGQINDLATEMYELQVVGLRHSAQ 58 Query: 59 AALAMAIHFRRGYSYIYPNANDPKSRVGTRALNQKAEAQIQDAFDYLQGAEK---SPQLE 115 A L ++ R + I A P R + LN + ++ + L AE+ +P+ + Sbjct: 59 ATLDLSSSNRAIRTAIL--AESPAEREAS--LNDLTK-RLDNTTLALNEAEQRFVTPEGK 113 Query: 116 PLLAALNQAWPAYRESFQRLMDKADQD---DVPGALAELQKTTDPAHVKLRDVFLQIVKQ 172 L+A +A+ Y+ + + ++ ++ D + + L + P + L+++ Sbjct: 114 ALVAKAREAFTKYKNAVMQTAEELRKEPLADARQSTSRLFSSVRPLANEAEANMLKLM-- 171 Query: 173 YDQAVQDHISRSAESVDRTAWYLVALLAAM----VVVGVAFSVVIQRSITRQLGGEPLYA 228 + +D+ R + D ++ LL + V+ G+A + I R++TRQLGGEP Sbjct: 172 --EVKKDNADRLNDETDLIYAHIRTLLLVLTFGGVLFGIALGLFISRNLTRQLGGEPRDV 229 Query: 229 VAIAQAIADGDLASRVKLRQGDQGSVLTAMESMRLRLSHLVQEVYKSCHAIDTGASEIAD 288 A A AIA GDL++ + + QGSV++AM M+ L +VQ V S +I TG+ +IA Sbjct: 230 AAAADAIASGDLSTHIDISHAAQGSVVSAMHMMQGSLRKVVQTVRTSSESIATGSRQIAT 289 Query: 289 GNLNLSRRTEEQASNLEQTAASMEQLTGSVKQNAQTVGEAVKRAGDASQEASQGGDAVAG 348 GNL+LS RTE+QAS LE+TA+SME+LT +VK NA A + A AS A +GG+ VA Sbjct: 290 GNLDLSARTEQQASALEETASSMEELTSTVKHNASNAQNAKEMAATASTVAQEGGEVVAQ 349 Query: 349 VVDLMGDIASSSARIKEIIAVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRTL 408 VVD M I SS ++ +II+VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVA+EVR L Sbjct: 350 VVDTMSAINESSRKMADIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVATEVRNL 409 Query: 409 AHRSAQAAREIKTLIEDSVDKVDSGYAKVQEASQAISGVVDRVRQVATLVTEIGVSTQEQ 468 A RSA AA+EIK LIEDSVDKV G +A +SG+V+ +V +++ EI ++ EQ Sbjct: 410 AQRSAAAAKEIKALIEDSVDKVTVGARLADQAGTTMSGIVENAGKVTSIIGEISAASTEQ 469 Query: 469 TQGIAQVGTAVEQLDNSTQQNAALVEQSAAAAESLSAQASKLLELMQVFKL 519 T GI Q+ A+ Q+D+ TQQNA+LVE++AAA+ +L QA L +++ +F L Sbjct: 470 TAGIEQINQAIMQMDDVTQQNASLVEEAAAASRALQDQADALAQIVSIFNL 520 >gi|300312783|ref|YP_003776875.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 542 Score = 305 bits (780), Expect = 8e-84, Method: Compositional matrix adjust. Identities = 175/337 (51%), Positives = 241/337 (71%), Gaps = 3/337 (0%) Query: 205 VGVAFSVVIQRSITRQLGGEPLYAVAIAQAIADGDLASRVKLRQGDQGSVLTAMESMRLR 264 + + +V++ R + +QLGGEP YA IAQ IADGDL + V+ + GD S+L A+++MR Sbjct: 198 ISILIAVLLTRGLLKQLGGEPTYAAQIAQQIADGDLTANVQTKTGDNSSLLFAIKNMRDS 257 Query: 265 LSHLVQEVYKSCHAIDTGASEIADGNLNLSRRTEEQASNLEQTAASMEQLTGSVKQNAQT 324 L +V +V + I+ +SEIA GNL+LS RTE+QAS+LE+TA++ME+L +VKQNA Sbjct: 258 LVSIVAQVRQGTFTIEQASSEIASGNLDLSSRTEQQASSLEETASAMEELMTTVKQNADN 317 Query: 325 VGEAVKRAGDASQEASQGGDAVAGVVDLMGDIASSSARIKEIIAVIDGIAFQTNILALNA 384 +A + A AS+ ASQGG+ VA VV M I +SS +I +II+VIDGIAFQTNILALNA Sbjct: 318 AKQASQLAVSASEVASQGGEVVAQVVTTMDGINASSRKIVDIISVIDGIAFQTNILALNA 377 Query: 385 AVEAARAGEQGRGFAVVASEVRTLAHRSAQAAREIKTLIEDSVDKVDSGYAKVQEASQAI 444 AVEAARAGEQGRGFAVVASEVR+LA RSA AA+EI LI DSV +++ G + V++A Q + Sbjct: 378 AVEAARAGEQGRGFAVVASEVRSLAQRSATAAKEITELINDSVSQIERGSSLVEQAGQTM 437 Query: 445 SGVVDRVRQVATLVTEIGVSTQEQTQGIAQVGTAVEQLDNSTQQNAALVEQSAAAAESLS 504 VV+ V++V +V EI +++QEQ+ GI QV TA+ +D +TQQN+ALVE++AAAA S+ Sbjct: 438 ENVVNSVKRVTDVVAEISMASQEQSHGITQVNTAITHMDEATQQNSALVEEAAAAANSMK 497 Query: 505 AQASKLLELMQVFKLPVGAHEQVNGAGPAIAAKPSAA 541 QA L L+ F+L +Q +G + A+ P+ A Sbjct: 498 EQAGALSGLVATFRLDA---QQARQSGYSQASAPAHA 531 >gi|300313891|ref|YP_003777983.1| methyl-accepting chemotaxis I protein [Herbaspirillum seropedicae SmR1] Length = 544 Score = 299 bits (766), Expect = 4e-82, Method: Compositional matrix adjust. Identities = 221/548 (40%), Positives = 323/548 (58%), Gaps = 38/548 (6%) Query: 1 MSFLQNLRVSARLALLSALFLVAMLVMVSWSIFSLHSISSQERQMYQE-----RIAPMQK 55 M+ L L++ +L L + +V ML+++++++ +++IS+ + YQE ++ P+ Sbjct: 1 MNQLSKLKIGTQLRLGFGIVIVLMLILITFALMRMNAISAAVQ--YQETVTDTKLEPLYV 58 Query: 56 LNTAALAMAIHFRRGYSYIYPNANDPKSRVGTRALNQKAEAQIQDAFDYLQGAEKSPQLE 115 A + R Y + +D + AL + +A DA QLE Sbjct: 59 AREALDQTGLAARNAYIF-----HDQAEALKELALLDQQKALYLDALR---------QLE 104 Query: 116 PLLAALNQ------AWPAYRESFQRLMDKADQDDVPGALAELQKTTDPAH---VKLRDVF 166 P+LA Q A E +R + + L K P V+ D Sbjct: 105 PVLAGQAQFEKAKAGLLAMAEELKRPRKYRESGQMEAYGEFLVKECSPLRRQIVQDIDAL 164 Query: 167 LQIVKQYDQAVQDHISRSAESVDRTAWYLVALLAAMVV-VGVAFSVVIQRSITRQLGGEP 225 L++V++ +A D A+++ ++ L+ AA+++ V +A + +I++S+ QLGGEP Sbjct: 165 LKVVQRETKAAGDR----ADALFASSLRLIMACAAIILGVSIATAWLIRKSLLGQLGGEP 220 Query: 226 LYAVAIAQAIADGDLASRVKLRQGDQGSVLTAMESMRLRLSHLVQEVYKSCHAIDTGASE 285 YAV I IA G+L++ V GD+ S+L + MR +L+ LVQ+V HAI +SE Sbjct: 221 RYAVEITGRIAHGELSAPVDTAPGDRSSLLYEIRVMRDKLASLVQQVRVGTHAIADASSE 280 Query: 286 IADGNLNLSRRTEEQASNLEQTAASMEQLTGSVKQNAQTVGEAVKRAGDASQEASQGGDA 345 IA GN NLS RTE+QA LE+T A+M++LT +V++NA +A AG AS ASQ G Sbjct: 281 IAVGNQNLSERTEQQADTLEKTVAAMDELTQAVRRNADNAQQAKHLAGSASDVASQAGTV 340 Query: 346 VAGVVDLMGDIASSSARIKEIIAVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEV 405 V VVD MG I +SS +I +II VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVA+EV Sbjct: 341 VQQVVDTMGQIDASSRKIVDIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAAEV 400 Query: 406 RTLAHRSAQAAREIKTLIEDSVDKVDSGYAKVQEASQAISGVVDRVRQVATLVTEIGVST 465 R+LA RSA AAREIK+LI+ SV V G V++A + VV+ V++V+ +V EI ++ Sbjct: 401 RSLAQRSASAAREIKSLIDSSVSSVTDGSRLVEQAGATMQDVVESVQRVSAVVVEISAAS 460 Query: 466 QEQTQGIAQVGTAVEQLDNSTQQNAALVEQSAAAAESLSAQASKLLELMQVFKLPVGAHE 525 Q Q+ GI +V TA+ +LD +TQQNAALVE++AAA++S+S QA L +L+ FKL + Sbjct: 461 QAQSDGILEVNTAIARLDENTQQNAALVEEAAAASQSMSQQADHLNQLVGAFKL---SPA 517 Query: 526 QVNGAGPA 533 Q+ G PA Sbjct: 518 QMEGQHPA 525 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.126 0.333 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,133,089 Number of extensions: 38680 Number of successful extensions: 550 Number of sequences better than 1.0e-08: 70 Number of HSP's gapped: 274 Number of HSP's successfully gapped: 71 Length of query: 545 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 455 Effective length of database: 1,193,720 Effective search space: 543142600 Effective search space used: 543142600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)