BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1550.p (845 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312548|ref|YP_003776640.1| filamentous hemagglutinin outer... 471 e-134 gi|300310626|ref|YP_003774718.1| filamentous hemagglutinin prote... 354 2e-98 >gi|300312548|ref|YP_003776640.1| filamentous hemagglutinin outer membrane protein [Herbaspirillum seropedicae SmR1] Length = 2685 Score = 471 bits (1213), Expect = e-134, Method: Compositional matrix adjust. Identities = 360/946 (38%), Positives = 537/946 (56%), Gaps = 127/946 (13%) Query: 18 RQLGDGFYEQQLVQRQIQQITGQRYLAGYSSNEAQYQALMNAGLQVAQAQRYTVGVALTD 77 ++LGDGFYEQ++V+ Q+ Q+TG+ +G ++++QYQ L+N + VAQ+ GVALT Sbjct: 1077 KRLGDGFYEQRMVREQMAQLTGRP--SGSFNDDSQYQKLLNNAVSVAQSWNLRPGVALTP 1134 Query: 78 AQVAALKTDIVWLVKQTVTLADGSTQEVLVPQVYV-HASNVEVTGQGTLIAGNDVAFQAA 136 QV+ L +DIVWL Q V L DG T+EVLVP++Y+ H + G L++G+++ QA Sbjct: 1135 DQVSHLTSDIVWLETQKVVLPDGRTEEVLVPKLYLAHIDKDALKNSGALVSGDNITIQA- 1193 Query: 137 QDIVNSGGTIAAR-----KGVSLAGANLQNLGGRISGADVQVAAAQDINNIG-------- 183 ++I N GG I R + V +A ++ NLGG ISG ++ ++A +++ N Sbjct: 1194 ENISNKGGFIDGRASGTGRTVLVASNDIANLGGGISGDEIVISAGRNVRNETLTTTQTYN 1253 Query: 184 --------------GTIDARNTLVATAGRDINSASTTVATANA----------------- 212 G+I A L TAG DI + TVA+ + Sbjct: 1254 NGQTSGSYTSLSNQGSIAADKNLTITAGADITNLGATVASGRSGDAATGKLSMSAGRDIN 1313 Query: 213 ---VTSGTNINQNAS------MSVSE------ANGSLTAVAGRDINLKAS----GTSAD- 252 V +G+ + N S SVS A G LT VA RD++L A+ GT+ Sbjct: 1314 LGTVKTGSTYDANFSGYFGHAQSVSSVGSTVNAGGDLTMVAQRDLSLTAAQVAIGTNGSG 1373 Query: 253 NVTLVAQRDVNLATVHETSQEKL---AWDNDNRAEVNRDNAIGSTVQGKD-ISVTAGR-- 306 N +L+A R V + + ++ + A + +RA+ GS + IS+ AG Sbjct: 1374 NGSLMAGRAVTIGAALDRAESTIGRAASRSYSRADDESVAVRGSNIDAAGGISIKAGLME 1433 Query: 307 --DINAQAVYVNAEGSLAANAGNNVNIGTDVSTANARDQHKKTDAGGVLSSRTVTTDDSS 364 D+ A + A +L A ++N+ ++ S + ++ K+ + G+LSS T T+ Sbjct: 1434 KADVKVAASSITAGKALEVAATGDINVVSEESF-QSHSEYAKSTSRGLLSSTTRTSYGKR 1492 Query: 365 SERINQGTTLSGNTVVVRAGKDINVTGSNVVATQGLGMAAGNDVNIVAA---VDSSTKNN 421 + G+TLSG+T V++AGKD+++ GSNVV++ G + A N++ + +A V+S+ +N Sbjct: 1493 AYTNAVGSTLSGDTTVLQAGKDMSIVGSNVVSSAGTSLVAANNITVASASDKVESAEGSN 1552 Query: 422 FRKETTSGVMGAGFGVTIGTREQSHDGKSQGQTASASTIGSTDGNVSIVAGNRYTQVGSD 481 + G GF TIGTRE + + TA +S +GS DGNVSI AGN YTQ GS+ Sbjct: 1553 KKSSGLLSGGGLGF--TIGTRELDNKNTTVNSTAVSSNVGSIDGNVSIKAGNAYTQSGSN 1610 Query: 482 VMAPKGDIDIAAKSVEIRAAEQASRTETEDKYKQSGLTVAVTSPVISAMQTVDQMVESSG 541 V+APKGDID+ AK + I AA + R+ + +KQSGLT+ +TSPV+SA+QT QM +++ Sbjct: 1611 VLAPKGDIDVVAKQISILAAAETERSTQDMVFKQSGLTIQITSPVLSAIQTAQQMAQAAK 1670 Query: 542 KTKNGRVKALAAATAGMSLYSAA------------------------------------- 564 T GR++ LAAA G + +A Sbjct: 1671 STSGGRMQLLAAANIGFAGKNAVDAVRTGQGFAVDGKDNPQVRTNALDENGDLKLGADKK 1730 Query: 565 GEMQKAGSAEGGSNIGISATIGSSQNGSRSEQNTVTQRGSTVASGGNMSIRATGDGANSN 624 E + A +A+ I ++ ++G+S++ + S+ + T RGST+ +GGN+S+ A G GA+SN Sbjct: 1731 PETRDATAADKAGGINLAISVGASKSENHSQSSASTARGSTLNAGGNISLTAQGGGADSN 1790 Query: 625 ITIAGSDINAKGNLALKADNDINLIAAQNSDEQHSTRSSSSWGVGITAQIGSQSKLGITA 684 + + GSDI + N LKADN++ L+AAQ++ EQ S+ + S VGI+ IG+ L + A Sbjct: 1791 VIVQGSDIRSGANTLLKADNEVRLLAAQSTTEQSSSNKAMSGSVGIS--IGTDGFL-VNA 1847 Query: 685 NAAGSRGKSDGKDVSNVMTQVRSGGQIKIDSGRDTNLIGATVSGEQVQANVGR----DLN 740 +GSRG+ DG DV+ V T V +G ++ I SG DT + GA SG+QV +VG +LN Sbjct: 1848 GLSGSRGRGDGSDVAQVNTHVDAGNKLTIASGTDTTIKGAVASGKQVVIDVGTSGSGNLN 1907 Query: 741 IASVQDTSTFKSRDQSIGGSATIGYGS-SASISASRSRVDADYASVGQQAGIMAGNGGFQ 799 I S+QDTST+KSR QS G S + G G S S++ +RS VD +YASV +Q+GI AG+ GFQ Sbjct: 1908 IESLQDTSTYKSRQQSAGVSISAGMGRMSGSLNLARSSVDGNYASVNEQSGIKAGDDGFQ 1967 Query: 800 INVKGNTDLKGAQIASTDQAVEQGRNSLTTGTLTSSDIQNRSQYSA 845 INV GNTDLKGA+IASTD+A G+NSLTT TLT SDI+NRS + A Sbjct: 1968 INVAGNTDLKGAKIASTDKAAADGKNSLTTETLTQSDIKNRSDFKA 2013 >gi|300310626|ref|YP_003774718.1| filamentous hemagglutinin protein [Herbaspirillum seropedicae SmR1] Length = 2658 Score = 354 bits (909), Expect = 2e-98, Method: Compositional matrix adjust. Identities = 274/720 (38%), Positives = 419/720 (58%), Gaps = 41/720 (5%) Query: 160 QNLGGRI-SGADVQVAAAQDINNIGGTIDARNTLVATAGRDINSASTTVATANAVTSGTN 218 Q LG I +G D AA ++I G +ID + AG + ++ AT + VT ++ Sbjct: 1332 QALGSTIEAGGDASFAAGRNIAVRGSSIDVKGNAALLAGGSVEVSAVKTATDSVVTGRSD 1391 Query: 219 INQNASMSVSEANGSLTAVAGRDINLKASGTSAD-NVTLVAQR------DVNLATVHETS 271 + +E + AG+ +++ ASG + ++TL A R +V L + S Sbjct: 1392 RYDLNVVGHTENLTTSQIAAGKGLSIVASGQNGSGDITLAASRLSSKEGEVTLNAAGDIS 1451 Query: 272 QEKLAWDNDNRAE--------VNRDN---AIGSTVQG-KDISVTA-GRD-----INAQAV 313 + +NDNR + D+ A GS ++G K +++ A GR + + Sbjct: 1452 IGAMQKNNDNRIDERGGKAWMKTHDSSSVAAGSLIEGEKGVNLIATGRSDAKVSVAGSDI 1511 Query: 314 YVNAEGSLAANAGNNVNIGTDVSTANARDQHKKTDAGGVLSSRTVTTDDSSSERINQGTT 373 Y N G++A A +V + S+++ Q +++ G LS + D + + G+T Sbjct: 1512 YSN--GTIAIQAKGDVTVAETRSSSDEW-QQAHSESRGFLSRSSSDRTDQVRQDMANGST 1568 Query: 374 LSGNTVVVRAGKDINVTGSNVVATQGLGMAAGNDVNIVAAVDSSTKNNFRKETTSGVMGA 433 LSGN VV+ AG++I V GS VV+T+ + A NDV+I + + +++++++ SG+MGA Sbjct: 1569 LSGNKVVIDAGRNIAVRGSEVVSTELTSLRAANDVSITTTQNFTEQSHYKEDRKSGLMGA 1628 Query: 434 GFGVTIGTREQSHDGKSQGQTASASTIGSTDGNVSIVAGNRYTQVGSDVMAPKGDIDIAA 493 GFG++IG++ Q +G T +AST+GS +GNV+I+AGN + Q+GS V+AP+GDI+I A Sbjct: 1629 GFGISIGSQGQMANGNGSSVTNNASTVGSVNGNVNIIAGNAFRQIGSQVVAPQGDINILA 1688 Query: 494 KSVEIRAAEQASRTETEDKYKQSGLTVAVTSPVISAMQTVDQMVESSGKTKNGRVKALAA 553 KSV+I AA AS E+K+KQSGLT+A+ SPVIS +QT Q++++ KT + R++ALAA Sbjct: 1689 KSVDIGAAYDASVYTQENKFKQSGLTLAINSPVISVLQTGQQLIQAGQKTPDKRMQALAA 1748 Query: 554 ATAGMSLYSA-AGEMQKAGSAEGGSNIGISATIGSSQNGSRSEQNTVTQRGSTVASGGNM 612 AT + Y A A + K G + I+ ++GSSQ+ ++ Q + GSTVA+GG + Sbjct: 1749 ATLAKNAYDAGAALIDKPTDTSG---VKITLSLGSSQSSNKVTQASSQAVGSTVAAGGAV 1805 Query: 613 SIRATGDGANSNITIAGSDINAKGNLALKADNDINLIAAQNSDEQHSTRSSSSWGVGI-T 671 +I A+G G S+I + GS I+A G+++L AD++INL+AAQ++ QH +SS +GI Sbjct: 1806 NIVASG-GPRSDINVVGSAISAGGDVSLVADHEINLLAAQSAYSQHGNNNSSGASIGIGF 1864 Query: 672 AQIGSQSKLGITANAAGSRGKSDGKDVSNVMTQVRSGGQIKIDSGRDTNLIGATVSGEQV 731 A GSQ+ + A +RG DG D+ N + + +G Q + SG DTN+ G+TVS + V Sbjct: 1865 AMGGSQNGFTLELGANRARGNLDGDDLVNKASTISAGNQASLISGGDTNVRGSTVSAKTV 1924 Query: 732 QANVGRDLNIASVQDTSTFKSRDQSIGGSATIGYGSSA------SISASRSRVDADYASV 785 A +G DLNI S+QD ST+ S+ S G ++ S S S S+ DY SV Sbjct: 1925 AAAIGGDLNIESLQDKSTYGSKQSSAGVGLSLCIPPFCYGASSVSGSLSGSKAKGDYQSV 1984 Query: 786 GQQAGIMAGNGGFQINVKGNTDLKGAQIASTDQAVEQGRNSLTTGTLTSSDIQNRSQYSA 845 QAGI AG+GGF++ V GNTDLKGA I+ST +A+E G+N L TGTLT SDI N Y A Sbjct: 1985 VTQAGIKAGDGGFRLVVAGNTDLKGAVISSTAKAIEDGKNFLQTGTLTYSDISNHDAYEA 2044 Score = 158 bits (399), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 114/342 (33%), Positives = 175/342 (51%), Gaps = 54/342 (15%) Query: 5 LGQLNQPNAAIGVRQLGDGFYEQQLVQRQIQQITGQRYLAGYSSNEAQYQALMNAGLQVA 64 LG+L + ++ ++LGDGFYEQ+L+ QI Q+TG+++L GY++ EAQY+ALM++G+ A Sbjct: 1073 LGRLTM-DPSMTQKRLGDGFYEQKLINDQIAQLTGKKFLDGYTNAEAQYKALMDSGVASA 1131 Query: 65 QAQRYTVGVALTDAQVAALKTDIVWLVKQTVTLADGSTQEVLVPQVYV-HASNVEVTGQG 123 + + T G+AL+ Q+AAL +DIVW+V VTLADGS Q LVP VY+ H + + G Sbjct: 1132 ASFKLTPGIALSADQMAALTSDIVWMVDTEVTLADGSRQHALVPTVYLAHGGSATLQASG 1191 Query: 124 TLIAGNDVAFQAAQDIVNSGGTIAARKGVSLAGANLQNLGGRISGADVQVAAAQDINNIG 183 LI G DV A D VN+ G + A +++A ++ N GG + G V V A + + NIG Sbjct: 1192 ALIGGRDVTL--AADTVNNSGIVRAEDKLAVAARDINNTGGELRGDKVDVLATETL-NIG 1248 Query: 184 GTIDARNTLVATAGRDINSASTTVATANAVTSGTNINQNASMSVSEANGSLTAVAGRDIN 243 T N + S++ G ++A DIN Sbjct: 1249 ------------------------------TDFQNYGRQGSVT-----GRQVSLAANDIN 1273 Query: 244 LKASGTSADN-VTLVAQRDVNLATVHETSQEKLAWDNDNRAEVNR------------DNA 290 + + AD+ + L AQ ++ +A KL W E N A Sbjct: 1274 IIGAKIKADDTLKLAAQNNLTIAAATAQHDVKLTWSGAGEREENHGMMRNGGVTAQLQQA 1333 Query: 291 IGSTVQ-GKDISVTAGRDINAQAVYVNAEGSLAANAGNNVNI 331 +GST++ G D S AGR+I + ++ +G+ A AG +V + Sbjct: 1334 LGSTIEAGGDASFAAGRNIAVRGSSIDVKGNAALLAGGSVEV 1375 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.306 0.122 0.319 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,241,970 Number of extensions: 91389 Number of successful extensions: 477 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 394 Number of HSP's successfully gapped: 6 Length of query: 845 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 752 Effective length of database: 1,179,515 Effective search space: 886995280 Effective search space used: 886995280 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.6 bits) S2: 135 (56.6 bits)