BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1549.p (2324 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312548|ref|YP_003776640.1| filamentous hemagglutinin outer... 966 0.0 gi|300310626|ref|YP_003774718.1| filamentous hemagglutinin prote... 492 e-139 >gi|300312548|ref|YP_003776640.1| filamentous hemagglutinin outer membrane protein [Herbaspirillum seropedicae SmR1] Length = 2685 Score = 966 bits (2498), Expect = 0.0, Method: Compositional matrix adjust. Identities = 612/1232 (49%), Positives = 810/1232 (65%), Gaps = 101/1232 (8%) Query: 735 NFQLDVSPTSGAGPNRANSVRAVPASVSGAAGASAGAASIRIASLDLTLPNNALYRINAG 794 N + D++ T+G+G SV +V S GA+A A + + PN +G Sbjct: 993 NIRSDLTLTTGSG----QSVGSVLNDASARPGATAPDAPVPVR------PNPMFVPATSG 1042 Query: 795 PAQRYLVQTDPQFVGTRNWLSSDFMLNQLGLQSGVRQ--LGDGFYEQQLVQRQIQQITGQ 852 YL++T QF R WLSSD++L L + Q LGDGFYEQ++V+ Q+ Q+TG+ Sbjct: 1043 ---NYLLETRTQFTDRRTWLSSDYLLTALNVDPSTTQKRLGDGFYEQRMVREQMAQLTGR 1099 Query: 853 RYLAGYSSNEAQYQALMNAGLQVAQAQRYTVGVALTDAQVAALKTDIVWLVKQTVTLADG 912 +G ++++QYQ L+N + VAQ+ GVALT QV+ L +DIVWL Q V L DG Sbjct: 1100 P--SGSFNDDSQYQKLLNNAVSVAQSWNLRPGVALTPDQVSHLTSDIVWLETQKVVLPDG 1157 Query: 913 STQEVLVPQVYV-HASNVEVTGQGTLIAGNDVAFQAAQDIVNSGGTIAAR-----KGVSL 966 T+EVLVP++Y+ H + G L++G+++ QA ++I N GG I R + V + Sbjct: 1158 RTEEVLVPKLYLAHIDKDALKNSGALVSGDNITIQA-ENISNKGGFIDGRASGTGRTVLV 1216 Query: 967 AGADLQNLGGRISGADVQVAAAQDINNIG----------------------GTIDARNSL 1004 A D+ NLGG ISG ++ ++A +++ N G+I A +L Sbjct: 1217 ASNDIANLGGGISGDEIVISAGRNVRNETLTTTQTYNNGQTSGSYTSLSNQGSIAADKNL 1276 Query: 1005 VATAGRDINSASTTVATANA--------------------VTSGTNINQNAS------MS 1038 TAG DI + TVA+ + V +G+ + N S S Sbjct: 1277 TITAGADITNLGATVASGRSGDAATGKLSMSAGRDINLGTVKTGSTYDANFSGYFGHAQS 1336 Query: 1039 VSE------ANGSLTAVAGRDINLKAS----GTSAD-NITLVAQRDVNISTVHETSQEKL 1087 VS A G LT VA RD++L A+ GT+ N +L+A R V I + ++ + Sbjct: 1337 VSSVGSTVNAGGDLTMVAQRDLSLTAAQVAIGTNGSGNGSLMAGRAVTIGAALDRAESTI 1396 Query: 1088 ---AWDNDNRAEVNHDNAIGSTVQGKD-ISVTAG----RDINAQAAYVNAEGSLAATAAN 1139 A + +RA+ GS + IS+ AG D+ A+ + A +L A Sbjct: 1397 GRAASRSYSRADDESVAVRGSNIDAAGGISIKAGLMEKADVKVAASSITAGKALEVAATG 1456 Query: 1140 NINIGTDVSTASARDQHKKTDAGGVLSSRTVTTDDSSSERINQGTTLSGNTVVVRAGKDI 1199 +IN+ ++ S S ++ K+ + G+LSS T T+ + G+TLSG+T V++AGKD+ Sbjct: 1457 DINVVSEESFQS-HSEYAKSTSRGLLSSTTRTSYGKRAYTNAVGSTLSGDTTVLQAGKDM 1515 Query: 1200 NVIGSNVVATQGVGMAAGNDVNIVAA---VDSSTKNNFRKETTSGVMGAGFGVTIGTREQ 1256 +++GSNVV++ G + A N++ + +A V+S+ +N + G GF TIGTRE Sbjct: 1516 SIVGSNVVSSAGTSLVAANNITVASASDKVESAEGSNKKSSGLLSGGGLGF--TIGTREL 1573 Query: 1257 SHDGKSQGQTASASTIGSTDGNVSIVAGNRYTQVGSDVMAPKGDIDIAAKSVEIRAAEQA 1316 + + TA +S +GS DGNVSI AGN YTQ GS+V+APKGDID+ AK + I AA + Sbjct: 1574 DNKNTTVNSTAVSSNVGSIDGNVSIKAGNAYTQSGSNVLAPKGDIDVVAKQISILAAAET 1633 Query: 1317 SKATTEDKFKQTGLTVAVTSPVISAIQTVGQMADAASKTKDGRMQALAAANMGFAGKNAY 1376 ++T + FKQ+GLT+ +TSPV+SAIQT QMA AA T GRMQ LAAAN+GFAGKNA Sbjct: 1634 ERSTQDMVFKQSGLTIQITSPVLSAIQTAQQMAQAAKSTSGGRMQLLAAANIGFAGKNAV 1693 Query: 1377 DAVKAGQGFAVEGKDNPQVLTNARNEDGSLKTGPDGNPETRDATAADKVGGINLALSLGS 1436 DAV+ GQGFAV+GKDNPQV TNA +E+G LK G D PETRDATAADK GGINLA+S+G+ Sbjct: 1694 DAVRTGQGFAVDGKDNPQVRTNALDENGDLKLGADKKPETRDATAADKAGGINLAISVGA 1753 Query: 1437 SSSESRSQQSSNTVRGSTVSAGGSINISAQGGGTDSNIVIQGSDIKAGVNATLKAANEVR 1496 S SE+ SQ S++T RGST++AGG+I+++AQGGG DSN+++QGSDI++G N LKA NEVR Sbjct: 1754 SKSENHSQSSASTARGSTLNAGGNISLTAQGGGADSNVIVQGSDIRSGANTLLKADNEVR 1813 Query: 1497 LLAAQNTSEQHSNNQSSSGSVGVSIGTDGLMFNASASGSRGRGDGSDVTQVNTHVDAGNK 1556 LLAAQ+T+EQ S+N++ SGSVG+SIGTDG + NA SGSRGRGDGSDV QVNTHVDAGNK Sbjct: 1814 LLAAQSTTEQSSSNKAMSGSVGISIGTDGFLVNAGLSGSRGRGDGSDVAQVNTHVDAGNK 1873 Query: 1557 LTIASGTDTTLKGAVVSGKQVVMDVGTSGKGNLNIESLQDTSTYKSKQQSLGGSISVGMG 1616 LTIASGTDTT+KGAV SGKQVV+DVGTSG GNLNIESLQDTSTYKS+QQS G SIS GMG Sbjct: 1874 LTIASGTDTTIKGAVASGKQVVIDVGTSGSGNLNIESLQDTSTYKSRQQSAGVSISAGMG 1933 Query: 1617 KMSGSLSASRSTVDGNFASVNEQSGIRAGDGGFQINVKGNTDLKGGKIASTDKAAAEGKN 1676 +MSGSL+ +RS+VDGN+ASVNEQSGI+AGD GFQINV GNTDLKG KIASTDKAAA+GKN Sbjct: 1934 RMSGSLNLARSSVDGNYASVNEQSGIKAGDDGFQINVAGNTDLKGAKIASTDKAAADGKN 1993 Query: 1677 SLTTETLTQSDIQNRSDYKAESQSASIGGGFSGGKSSMNGTGIGVGSSSGSESSTTKSGI 1736 SLTTETLTQSDI+NRSD+KAESQS +G +G + G+ +G G+ SGS+SSTT+SGI Sbjct: 1994 SLTTETLTQSDIKNRSDFKAESQSVGMGTNLAGSVLGLAGSALGRGNVSGSDSSTTRSGI 2053 Query: 1737 SQANIKITDDAAQQAKTGKTAEQTIASINTDVSSDRDTSGKLTKQWDAQALQADVQAQAQ 1796 SQA+IKITDDAAQQAKTGKTAEQTIASIN DVSS+RDTSGK+ K W+ Q LQADV+AQA+ Sbjct: 2054 SQADIKITDDAAQQAKTGKTAEQTIASINADVSSERDTSGKIAKTWNGQELQADVEAQAE 2113 Query: 1797 ITEMFGKNAAKEIGSYATTKVNEL-NAKIDVAQSEGEKAALI--AERDKWDEGGLYRTAL 1853 IT FG+ A+ G+YA +K EL A + AQ+ + AL+ AE D+W +GG + AL Sbjct: 2114 ITRAFGQQVAQLAGAYAESKEKELRQAASNAAQNGDQTQALLLGAEADQWKDGGENKRAL 2173 Query: 1854 HTAAGLLGGGVDG-AIGAATSSVAMPQLAKLI 1884 L+ GG+ G + G ++ + P LA I Sbjct: 2174 KAITTLVIGGLGGQSAGQLLANASAPYLADAI 2205 >gi|300310626|ref|YP_003774718.1| filamentous hemagglutinin protein [Herbaspirillum seropedicae SmR1] Length = 2658 Score = 492 bits (1267), Expect = e-139, Method: Compositional matrix adjust. Identities = 394/1014 (38%), Positives = 571/1014 (56%), Gaps = 100/1014 (9%) Query: 972 QNLGGRI-SGADVQVAAAQDINNIGGTIDARNSLVATAGRDINSASTTVATANAVTSGTN 1030 Q LG I +G D AA ++I G +ID + + AG + ++ AT + VT ++ Sbjct: 1332 QALGSTIEAGGDASFAAGRNIAVRGSSIDVKGNAALLAGGSVEVSAVKTATDSVVTGRSD 1391 Query: 1031 INQNASMSVSEANGSLTAVAGRDINLKASGTSAD-NITLVAQR------DVNISTVHETS 1083 + +E + AG+ +++ ASG + +ITL A R +V ++ + S Sbjct: 1392 RYDLNVVGHTENLTTSQIAAGKGLSIVASGQNGSGDITLAASRLSSKEGEVTLNAAGDIS 1451 Query: 1084 QEKLAWDNDNRAE--------VNHDN---AIGSTVQG-KDISVTA-GR---DINAQAAYV 1127 + +NDNR + HD+ A GS ++G K +++ A GR ++ + + Sbjct: 1452 IGAMQKNNDNRIDERGGKAWMKTHDSSSVAAGSLIEGEKGVNLIATGRSDAKVSVAGSDI 1511 Query: 1128 NAEGSLAATAANNINIGTDVSTASARDQHKKTDAGGVLSSRTVTTDDSSSERINQGTTLS 1187 + G++A A ++ + + ++S Q +++ G LS + D + + G+TLS Sbjct: 1512 YSNGTIAIQAKGDVTV-AETRSSSDEWQQAHSESRGFLSRSSSDRTDQVRQDMANGSTLS 1570 Query: 1188 GNTVVVRAGKDINVIGSNVVATQGVGMAAGNDVNIVAAVDSSTKNNFRKETTSGVMGAGF 1247 GN VV+ AG++I V GS VV+T+ + A NDV+I + + +++++++ SG+MGAGF Sbjct: 1571 GNKVVIDAGRNIAVRGSEVVSTELTSLRAANDVSITTTQNFTEQSHYKEDRKSGLMGAGF 1630 Query: 1248 GVTIGTREQSHDGKSQGQTASASTIGSTDGNVSIVAGNRYTQVGSDVMAPKGDIDIAAKS 1307 G++IG++ Q +G T +AST+GS +GNV+I+AGN + Q+GS V+AP+GDI+I AKS Sbjct: 1631 GISIGSQGQMANGNGSSVTNNASTVGSVNGNVNIIAGNAFRQIGSQVVAPQGDINILAKS 1690 Query: 1308 VEIRAAEQASKATTEDKFKQTGLTVAVTSPVISAIQTVGQMADAASKTKDGRMQALAAAN 1367 V+I AA AS T E+KFKQ+GLT+A+ SPVIS +QT Q+ A KT D RMQALAAA Sbjct: 1691 VDIGAAYDASVYTQENKFKQSGLTLAINSPVISVLQTGQQLIQAGQKTPDKRMQALAAAT 1750 Query: 1368 MGFAGKNAYDAVKAGQGFAVEGKDNPQVLTNARNEDGSLKTGPDGNPETRDATAADKVGG 1427 + KNAYDA G A+ D P G Sbjct: 1751 L---AKNAYDA-----GAAL--IDKPT-----------------------------DTSG 1771 Query: 1428 INLALSLGSSSSESRSQQSSNTVRGSTVSAGGSINISAQGGGTDSNIVIQGSDIKAGVNA 1487 + + LSLGSS S ++ Q+S+ GSTV+AGG++NI A GG S+I + GS I AG + Sbjct: 1772 VKITLSLGSSQSSNKVTQASSQAVGSTVAAGGAVNIVAS-GGPRSDINVVGSAISAGGDV 1830 Query: 1488 TLKAANEVRLLAAQNTSEQHSNNQSSSGSVGVSIGT----DGLMFNASASGSRGRGDGSD 1543 +L A +E+ LLAAQ+ QH NN SS S+G+ +G A+ +RG DG D Sbjct: 1831 SLVADHEINLLAAQSAYSQHGNNNSSGASIGIGFAMGGSQNGFTLELGANRARGNLDGDD 1890 Query: 1544 VTQVNTHVDAGNKLTIASGTDTTLKGAVVSGKQVVMDVGTSGKGNLNIESLQDTSTYKSK 1603 + + + AGN+ ++ SG DT ++G+ VS K V +G G+LNIESLQD STY SK Sbjct: 1891 LVNKASTISAGNQASLISGGDTNVRGSTVSAKTVAAAIG----GDLNIESLQDKSTYGSK 1946 Query: 1604 QQSLGGSISVGMGKMS-----GSLSASRSTVDGNFASVNEQSGIRAGDGGFQINVKGNTD 1658 Q S G +S+ + S S S S G++ SV Q+GI+AGDGGF++ V GNTD Sbjct: 1947 QSSAGVGLSLCIPPFCYGASSVSGSLSGSKAKGDYQSVVTQAGIKAGDGGFRLVVAGNTD 2006 Query: 1659 LKGGKIASTDKAAAEGKNSLTTETLTQSDIQNRSDYKAESQSASIGGGFS---------- 1708 LKG I+ST KA +GKN L T TLT SDI N Y+A S S G S Sbjct: 2007 LKGAVISSTAKAIEDGKNFLQTGTLTYSDISNHDAYEASGYSVS--GSMSSQLGDQSKAK 2064 Query: 1709 --GGKSSMN-----GTGIGVGSSSGSESSTTKSGISQANIKITDDAAQQAKTGKTAEQTI 1761 K++ N G G + GS+SS T+SGIS + ITD+ Q+A TG++AEQ + Sbjct: 2065 TEADKAAANSRTGPGGSPGYSDTGGSQSSVTRSGISGGTVIITDEQGQKAATGQSAEQIL 2124 Query: 1762 ASINTDVSSDRDTSGKLTKQWDAQALQADVQAQAQITEMFGKNAAKEIGSYATTKVNELN 1821 AS+N DV S +D+SG L+K WD +AL+ V+A+AQI + FG+ AK IG YA TK +L Sbjct: 2125 ASLNRDVDSSKDSSGALSKAWDGEALRQQVEAEAQIRQAFGQTVAKSIGDYADTKERDLK 2184 Query: 1822 AKIDVAQSEG---EKAALIAERDKWDEGGLYRTALHTAAGLLGGGVDGAIGAATSSVAMP 1878 A+ +G + A L AE +W E G R A+H AAG L GG+ GA AA S MP Sbjct: 2185 NAAKQAEQDGDAEQAAKLRAEAARWGEDGTARAAVHAAAGALSGGLAGAASAAAVSKLMP 2244 Query: 1879 QLAKLIDNTELPLPVRQVLAQAAAAALGGIAGGTSGLAAGIHVEANNRQLHETE 1932 LA+ I LP + V+A AAA+ LG I GG++G A + V+ NNRQLH++E Sbjct: 2245 SLAEQIKKMGLPSELEGVVAAAAASGLGAIIGGSAGAATAVTVDLNNRQLHQSE 2298 Score = 213 bits (543), Expect = 1e-55, Method: Compositional matrix adjust. Identities = 168/526 (31%), Positives = 260/526 (49%), Gaps = 78/526 (14%) Query: 643 VVTKSDPGMITSGGNMNLAVGALNNYASQVIAGGTLAGDSVNGTA-----INNTGPLGMQ 697 + SD + + G + + AL N AS +IAGG L + NG+A +NN G + Sbjct: 903 ITAMSDAAKLNASGRITINTAALTNDASDIIAGGALTINGANGSAASGGVVNNIG-YSIN 961 Query: 698 RVVSTGSAVFTYIKSHRFSADDRRYDGVPYQSQDI--VTNFQ-LDVSPTSGAGPNRANSV 754 R + G+AV Y DR Y + ++ VT Q +D + S N+A S+ Sbjct: 962 RT-AIGTAVDHY---------DRHVGHHTYTTLNLTEVTALQTIDANIQS----NQAVSI 1007 Query: 755 RAVPASVSGAAGASAGAASIRIASLDLTLPNNALYRINAGPAQRYLVQTDPQFVGTRNWL 814 A + + A+ + + ++TLP L+ + P QRYLV+TD +F + +L Sbjct: 1008 SAQNLNNTTVGAATVPTRAGGVGHANITLPAGGLFTVRPEPGQRYLVETDRRFTDYKTFL 1067 Query: 815 SSDFMLNQLGLQSGVRQ--LGDGFYEQQLVQRQIQQITGQRYLAGYSSNEAQYQALMNAG 872 SD+ML +L + + Q LGDGFYEQ+L+ QI Q+TG+++L GY++ EAQY+ALM++G Sbjct: 1068 GSDYMLGRLTMDPSMTQKRLGDGFYEQKLINDQIAQLTGKKFLDGYTNAEAQYKALMDSG 1127 Query: 873 LQVAQAQRYTVGVALTDAQVAALKTDIVWLVKQTVTLADGSTQEVLVPQVYV-HASNVEV 931 + A + + T G+AL+ Q+AAL +DIVW+V VTLADGS Q LVP VY+ H + + Sbjct: 1128 VASAASFKLTPGIALSADQMAALTSDIVWMVDTEVTLADGSRQHALVPTVYLAHGGSATL 1187 Query: 932 TGQGTLIAGNDVAFQAAQDIVNSGGTIAARKGVSLAGADLQNLGGRISGADVQVAAAQDI 991 G LI G DV A D VN+ G + A +++A D+ N GG + G V V A + + Sbjct: 1188 QASGALIGGRDVTL--AADTVNNSGIVRAEDKLAVAARDINNTGGELRGDKVDVLATETL 1245 Query: 992 NNIGGTIDARNSLVATAGRDINSASTTVATANAVTSGTNINQNASMSVSEANGSLTAVAG 1051 NIG T N + S++ G ++A Sbjct: 1246 -NIG------------------------------TDFQNYGRQGSVT-----GRQVSLAA 1269 Query: 1052 RDINLKASGTSADN-ITLVAQRDVNISTVHETSQEKLAWDNDNRAEVNH----------- 1099 DIN+ + AD+ + L AQ ++ I+ KL W E NH Sbjct: 1270 NDINIIGAKIKADDTLKLAAQNNLTIAAATAQHDVKLTWSGAGEREENHGMMRNGGVTAQ 1329 Query: 1100 -DNAIGSTVQ-GKDISVTAGRDINAQAAYVNAEGSLAATAANNINI 1143 A+GST++ G D S AGR+I + + ++ +G+ A A ++ + Sbjct: 1330 LQQALGSTIEAGGDASFAAGRNIAVRGSSIDVKGNAALLAGGSVEV 1375 Score = 75.1 bits (183), Expect = 6e-14, Method: Compositional matrix adjust. Identities = 132/433 (30%), Positives = 195/433 (45%), Gaps = 77/433 (17%) Query: 34 IQAQGTVDIAAATLVNAGLVSSNSDINLNTATLDNRAGSIGATGKLTVQGNSVTNSGGQL 93 + AQG +DI AA L + G +++ D +AG++ G LTV T SG L Sbjct: 344 LSAQGNLDITAAQLRSTGTLAAGVD----------QAGNVAQAGNLTV-----TASGDVL 388 Query: 94 QA---KQGVDIQAGSGLVDNSGGLLRSDGTVNVQAGSVRND---------NTQGQDQGVE 141 + G D+Q G +D L S + + G+ +D T D + Sbjct: 389 ATGVNRAGADLQLVGGKID----LSHSSSSAGRKLGAQASDALTLGGATVETLAGDLELG 444 Query: 142 GATVNV--------------AATDISNRQGAV-RGDAITLTASGRIDNGNGLISATN--S 184 GAT+ + A +SNR G + A+ +TA+ +DN G+ISA + Sbjct: 445 GATITLDGAKLTSSADARINAVAGLSNRGGEITSAGAMNVTAA-SVDNSAGMISAATGGA 503 Query: 185 AVLTDANPSTRTL-AIDNSNGTIIAGRQTTLLAYSFTGSGRVLSQKDLRMDLVASILHTG 243 VL S R+ +I N G I AG L A S G G V++ D + L + G Sbjct: 504 GVL-----SLRSAGSISNGQGRIAAGAALNLAAASIQGQGSVIANGDATLSLQSLNNEAG 558 Query: 244 Q-IGATAELDLRTAGTFTNAGSVGAGGTLTLTAATIDNQASG--SLVGNTLRLKATDVHT 300 I + +L + T+G N G++ A G L LTA+ IDN A G S TL+ D++ Sbjct: 559 SLIQSNRDLRITTSGATGNQGALKAIGALNLTASQIDNAAGGDISAAATTLQALNGDIN- 617 Query: 301 FINRGLIDGVNTIIESSTVNNLGTGRIYGDNIAIGADVLNNQAETVDGVTSAPVIA-ARN 359 N G I G S+ +NN G+ + G ++ I AD LNN + V A R+ Sbjct: 618 --NAGKIGGNTIATTSNALNNQGS--MIGGDVTINADRLNNSGAAAAIAATNSVQAFVRS 673 Query: 360 RLDIGAGVINNSEHGLIYSVGDMVIGGALDANKKVVGSARE----VNNSSATINADGNLS 415 L N ++ LIYS+G +VI A DA + G + NSSATI A G+L+ Sbjct: 674 AL-------TNQDNALIYSLGKLVI--AADATQSAAGDYLHRTGTLTNSSATIEAGGDLA 724 Query: 416 IAAGSINNNNAHL 428 ++A +I N + L Sbjct: 725 VSADTIVNKRSTL 737 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.310 0.126 0.347 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 6,264,756 Number of extensions: 254902 Number of successful extensions: 949 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 850 Number of HSP's successfully gapped: 8 Length of query: 2324 Length of database: 1,619,870 Length adjustment: 101 Effective length of query: 2223 Effective length of database: 1,141,635 Effective search space: 2537854605 Effective search space used: 2537854605 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.7 bits) S2: 139 (58.2 bits)