BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1542.p (456 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314020|ref|YP_003778112.1| major facilitator superfamily p... 357 1e-99 gi|300309872|ref|YP_003773964.1| major facilitator superfamily p... 249 3e-67 gi|300310323|ref|YP_003774415.1| methylenomycin A resistance pro... 125 5e-30 gi|300312966|ref|YP_003777058.1| major facilitator superfamily p... 113 3e-26 gi|300311185|ref|YP_003775277.1| major facilitator superfamily p... 112 5e-26 >gi|300314020|ref|YP_003778112.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 475 Score = 357 bits (915), Expect = 1e-99, Method: Compositional matrix adjust. Identities = 210/429 (48%), Positives = 285/429 (66%), Gaps = 5/429 (1%) Query: 4 SAPSLSLHTNHDGLPPRQRLLAIATVVLGLSIAVLDGSIANLALPLMAADLKASDADAIW 63 +APS + +GLPP QR AI + + + +A LD +IAN ALP MA DL+ + A ++W Sbjct: 11 AAPSPHIVIAGEGLPPEQRGRAILALGIAVGLATLDTAIANTALPAMALDLQTTPAASVW 70 Query: 64 VINSYQIAMAVCLLPLASLGEVIGYRRVYLGGVFLFTLASLACAASGDMLQLSLARVLQG 123 ++ +YQ+AM V LLPLA+LGE+IGYRR+Y+ G+ LFT ASL CA + + L +AR LQG Sbjct: 71 IVTAYQLAMMVALLPLAALGEIIGYRRIYIWGLVLFTAASLLCAVAWSLPTLVMARFLQG 130 Query: 124 LGAAGILSVNTALIRFIYPSRLLGRAIGYNALVAGTGNALGPTVAGLILHLATWHWLFLI 183 LG AGI+SVNTALIRFIYP+R LGR +G N L+ +GP+VA IL +A W WLF + Sbjct: 131 LGGAGIMSVNTALIRFIYPTRSLGRGVGLNTLIVAIAFTVGPSVASGILAIAPWPWLFAV 190 Query: 184 NIPLGLAALAVGHRALPTNPLSARPFDRQSALLCMCFIGLLLYTIDAVGHLQGAWVVGGC 243 N+PLG+ A+ + +LP L+ FD +SALL GLL+ I H A VV G Sbjct: 191 NVPLGVLAVVLAVPSLPHTSLARHAFDLRSALLNAAAFGLLILAIGEGAHQAQASVV-GL 249 Query: 244 TVLTAI--SLVLLLRLQSSSAPMLPLDLLRIPLFALSIGTSFCSFAAQMAVFVMLPFMLQ 301 + AI L LL R S APMLP+DL R P+F+LS T+ CSFAAQ FV LPF Sbjct: 250 ELAAAILCGLFLLKRELSHPAPMLPVDLFRHPMFSLSAVTAVCSFAAQGLAFVSLPFFFH 309 Query: 302 NQQGMSVARAGLLLTAWPIAVALTGVMAGKLSDRIAAGLLGGIGLAVMCLGLLSLLLVQP 361 + G S GLL+T WP+AVA+ +AG++SDR G+LGG+GLA++C GLLS+ + P Sbjct: 310 HDLGRSQVEIGLLMTPWPVAVAIMAPIAGRMSDRYPVGMLGGVGLALLCAGLLSMTFM-P 368 Query: 362 GTPDGVMVA-MFALCGVGFGLFQTPNNRTMISAAPRERTGGASGMLGTARLTGQTVGASV 420 +P ++ LCG+GFG FQ+PN + ++++AP R+GGASG++ TARL GQ+VGA++ Sbjct: 369 ASPSVWEISWRMLLCGMGFGFFQSPNLKALMTSAPPHRSGGASGIVATARLLGQSVGAAL 428 Query: 421 VAMLLALSN 429 VA+ LS Sbjct: 429 VALCFNLSQ 437 >gi|300309872|ref|YP_003773964.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 493 Score = 249 bits (637), Expect = 3e-67, Method: Compositional matrix adjust. Identities = 162/433 (37%), Positives = 237/433 (54%), Gaps = 11/433 (2%) Query: 8 LSLHTNHDGLPPRQRLLAIATVVLGLSIAVLDGSIANLALPLMAADLKASDADAIWVINS 67 S H N +GLP +QR A+ V+ ++AV DG+I N+ALP ++ L+ S A +IWV N+ Sbjct: 13 FSDHPNDEGLPGKQRRRAMLAVMTATTMAVFDGTIINVALPQISQALQTSAAVSIWVSNA 72 Query: 68 YQIAMAVCLLPLASLGEVIGYRRVYLGGVFLFTLASLACAASGDMLQLSLARVLQGLGAA 127 Y +A A+ L A+L IG+R + G+ +FTLASL CA SG + L L R+LQGLG A Sbjct: 73 YLLATAMTLAAFAALSSRIGFRAQFSAGLAVFTLASLGCALSGSVTLLILMRILQGLGGA 132 Query: 128 GILSVNTALIRFIYPSRLLGRAIGYNALVAGTGNALGPTVAGLILHLATWHWLFLINIPL 187 +LS+ A+ R I+P+RLLGR +G NA++ A+GP + G +L + W W+F IN+PL Sbjct: 133 AMLSIAPAIHRTIFPNRLLGRILGLNAVLVAASTAVGPVLGGTLLAMLGWEWIFAINVPL 192 Query: 188 GLAALAVGHRALPTNPLSAR-PFDRQSALLCMCFIGLLLYTIDAVGHL----QGA----W 238 G+ A+ + A+P A+ FD AL +G L+ D QG+ Sbjct: 193 GVLAVWLAWNAVPDTRKPAQAAFDLAGALWSALAMGALIMAADTCARFAEPGQGSGAAFT 252 Query: 239 VVGGCTVLTAISLVLLLRLQSSSAPMLPLDLLRIPLFALSIGTSFCSFAAQMAVFVMLPF 298 L + + ++ P+LPLD+ P F+L+ TS SF Q FV LPF Sbjct: 253 AAAYAVTALLAGLAFVRGQRRAADPLLPLDIFANPRFSLAALTSLASFVGQGIAFVALPF 312 Query: 299 MLQNQQGMSVARAGLLLTAWPIAVALTGVMAGKLSDRIAAGLLGGIGLAVMCLGLLSLLL 358 + QN G + + L T WP+ + L AG+L+DR + L + L LL Sbjct: 313 LFQNAYGYTAFESAALFTPWPLGIVLVAPHAGRLADRHSP-ALLSSLGLALFALGLVLLA 371 Query: 359 VQPGTPDGVMVAMFAL-CGVGFGLFQTPNNRTMISAAPRERTGGASGMLGTARLTGQTVG 417 + P + AL CG+GFG FQ+PNNR M+ RER+G ASG+L R GQ +G Sbjct: 372 LLPDQAQPWDIGWRALVCGMGFGFFQSPNNREMLGNVSRERSGNASGVLAIMRTFGQCLG 431 Query: 418 ASVVAMLLALSNA 430 A+++ ++LA+ A Sbjct: 432 AALLGVILAVRTA 444 >gi|300310323|ref|YP_003774415.1| methylenomycin A resistance protein [Herbaspirillum seropedicae SmR1] Length = 478 Score = 125 bits (315), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 99/326 (30%), Positives = 163/326 (50%), Gaps = 15/326 (4%) Query: 17 LPPRQRLLAIATVVLGLSIAVLDGSIANLALPLMAADLKASDADAIWVINSYQIAMAVCL 76 L PR +L + + +G +A+LD ++ N+ L +A L A + +W+++ Y + A L Sbjct: 23 LTPRAGMLPLVALAIGFVMAMLDVTVVNVGLSNIAGSLDAPLSTLVWIVDGYTLTFAAML 82 Query: 77 LPLASLGEVIGYRRVYLGGVFLFTLASLACAASGDMLQLSLARVLQGLGAAGILSVNTAL 136 L +L + G RR+YL G+FLF LASL C A+ + L +AR+LQGLGAA + + +L Sbjct: 83 LVGGALADRFGARRLYLTGLFLFVLASLLCGAAPNGPFLIVARLLQGLGAAFFMPSSLSL 142 Query: 137 IRFIYP-SRLLGRAIGYNALVAGTGNALGPTVAGLILHLATWHWLFLINIPLGLAALAVG 195 + +Y R+ R +G + G A+GP V G+++H W +FLIN+P+GL L + Sbjct: 143 LTHVYEDDRVRARMLGVWSATVGLAAAVGPLVGGVLIHWLGWRSVFLINVPVGLVGLVMA 202 Query: 196 HRALPTNPLSARPFDRQSALLCMCFIGLLLYTIDAVGHLQG---AW----VVGGCTVLTA 248 + +P AR + S L + + L + + +QG W ++ G V + Sbjct: 203 RKLIPQVGGHARALNLSSHLAGVVMLAGLSFVL-----IQGPVYGWTSPRILAGLAVALS 257 Query: 249 ISLVLLLRLQSSSAPMLPLDLLRIPLFALSIGTSF-CSFAAQMAVFVMLPFMLQNQQGMS 307 L+ AP+LP L P FA + G F +F +F + F LQ +G Sbjct: 258 AGAALIRHELRGIAPLLPKALFATPQFAAANGIGFLINFGVYGNLFYLALF-LQQGRGAD 316 Query: 308 VARAGLLLTAWPIAVALTGVMAGKLS 333 + GL L + +M+G++S Sbjct: 317 ALQTGLQLVPMMAVIFFGNLMSGRMS 342 >gi|300312966|ref|YP_003777058.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 544 Score = 113 bits (283), Expect = 3e-26, Method: Compositional matrix adjust. Identities = 124/400 (31%), Positives = 190/400 (47%), Gaps = 16/400 (4%) Query: 28 TVVLGLSIAVLDGSIANLALPLMAADLKASDADAIWVINSYQIAMAVCLLPLASLGEVIG 87 T G ++ VLD ++ + LP +A DL AS + WV++SY + A LLP + + G Sbjct: 40 TGSFGCAMTVLDTNVVGIILPTVARDLGASFSQIEWVVSSYVLCFAALLLPAGATADRYG 99 Query: 88 YRRVYLGGVFLFTLASLACAASGDMLQLSLARVLQGLGAAGILSVNTALI-RFIYPSRLL 146 RRV LGG+ LF L SLACA + L AR LQG+GAA +L+ A+I + Sbjct: 100 RRRVLLGGIALFALTSLACALAPSAQALYGARALQGVGAAFLLAPALAVIGHAFHDEDQR 159 Query: 147 GRAIGYNALVAGTGNALGPTVAGLILHLATWHWLFLINIP--LGLAALAVGHRALPTNPL 204 RA + G L P + G++ L W W F +N+P L LA H NPL Sbjct: 160 ERAWALWGGIMGLTMVLAPLIGGVVNTLLGWRWAFAVNLPACLLLAVAVWRHVPESRNPL 219 Query: 205 SARPFDRQSALLCMCFIGLLLYTIDAVGHLQGAWVVGGCTV----LTAISLVLLLRLQSS 260 RP D L + LL + + + + W C + ++ + + + Sbjct: 220 P-RPLDPLGILSSSSAVFLLTWAL--ITGPEHGWTSVACVARGLGGALLLVLFIAWQRRA 276 Query: 261 SAPMLPLDLLRIPLFALSIGTSFCSFAAQMAVFVMLPFMLQNQQGMSVARAGLLLTAWPI 320 + PML L L R F ++ F A+ + +LP LQN +G+S + GL + + + Sbjct: 277 AHPMLELSLFRHAGFVAAVAAMFAYAASAQVMASLLPLFLQNARGLSAWQTGLAMLPFAL 336 Query: 321 AVALTGVMAGKLSDRIAAGLLGGIGLAVMCLGLLSLLLVQ---PGTPDGVMVAMFALCGV 377 A+ L + +L+ +++ + +GL V LG +LL++Q G + M A+ G Sbjct: 337 AMLLLPQVGSRLARWLSSPQMLALGLGVTALG--NLLMIQAALSGAAAWTALGM-AVLGS 393 Query: 378 GFGLFQTPNNRTMISAAPRERTGGASGMLGTARLTGQTVG 417 G GL + ++ PRER G ASG+ TAR TG G Sbjct: 394 GGGLLNGQTQKAIMGNVPRERAGMASGISTTARFTGVLAG 433 >gi|300311185|ref|YP_003775277.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 532 Score = 112 bits (281), Expect = 5e-26, Method: Compositional matrix adjust. Identities = 120/433 (27%), Positives = 198/433 (45%), Gaps = 24/433 (5%) Query: 9 SLHTNHDGLPPRQRLLAIATVVLGLSIAVLDGSIANLALPLMAADLKASDADAIWVINSY 68 S H++ LP R+ LLA + + + LD +I ALP + A+LK + A WV +Y Sbjct: 7 SAHSSGQVLPFRESLLATLGICFVIMLVALDQTIVGTALPTIVAELKGFELYA-WVATAY 65 Query: 69 QIAMAVCLLPLASLGEVIGYRRVYLGGVFLFTLASLACAASGDMLQLSLARVLQGLGAAG 128 + + + LG+ G + + + +F AS C + ML L LAR LQG+G Sbjct: 66 LLTSVITVPIFGRLGDYFGRKPFVIASIIVFVGASALCGMANSMLFLVLARGLQGIGGGM 125 Query: 129 ILSVNTALIRFIYPS-RLLGRAIGYNALVAGTGNALGPTVAGLILHLATWHWLFLINIPL 187 ++ A + ++P R+ R ++ G A+GP++ G + W W+F N+P+ Sbjct: 126 LVGTCFASVADLFPDPRVRLRWQIILSVSFGIATAVGPSLGGFLTQGLGWRWVFYCNLPI 185 Query: 188 GLAALAVGHRALPT----NPLSARPFDRQSALLCMCFIGLLLYTIDAVGHLQGAWVVGGC 243 G+ +L R +P D ALL +G L ++ L V G Sbjct: 186 GVISLFFVWRFVPHIRHMQHAGKMRLDWPGALLISLSLGSLQLLVEL---LPKRGVTSGM 242 Query: 244 TVLTAISLVLLLRL----QSSSAPMLPLDLLR----IPLFALSIGTSFCSFAAQMAVFVM 295 VL A+S+ L Q ++ P+LP ++LR LF LS+ F F+ M Sbjct: 243 LVLLAVSVAAFYALWKWEQRAAQPILPFEMLRDRALASLFLLSVLAGFAMFSMLMYA--- 299 Query: 296 LPFMLQNQQGMSVARAGLLLTAWPIAVALTGVMAGKLSDRIA-AGLLGGIGLAVMCLGLL 354 P + Q G+S AGLL+T + + ++ G++ RI L+ +G A++ L Sbjct: 300 -PLLFQGGFGLSPREAGLLITPLVACITVASIVNGRIITRIPNPNLMMNLGFALIAASCL 358 Query: 355 SLLLVQPGTPDGVMVAMFALCGVGFGLFQTPNNRTMI-SAAPRERTGGASGMLGTARLTG 413 ++L T G ++A+ G G GL PN AA RE G A+ +L + R+ G Sbjct: 359 GIVLCDRVTGKGFLLAVMLAGGFGLGLV-LPNLTVFAQQAAAREHLGIATALLQSLRMIG 417 Query: 414 QTVGASVVAMLLA 426 +G ++ L++ Sbjct: 418 GMLGTAITGTLVS 430 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.139 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,292,690 Number of extensions: 52685 Number of successful extensions: 413 Number of sequences better than 1.0e-08: 15 Number of HSP's gapped: 397 Number of HSP's successfully gapped: 15 Length of query: 456 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 368 Effective length of database: 1,203,190 Effective search space: 442773920 Effective search space used: 442773920 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)