BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1525.p (281 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310979|ref|YP_003775071.1| alkanesulfonates ABC transporte... 436 e-123 gi|300309985|ref|YP_003774077.1| nitrate/sulfonate/bicarbonate A... 190 9e-50 gi|300309716|ref|YP_003773808.1| nitrate/sulfonate/bicarbonate A... 159 4e-40 gi|300311644|ref|YP_003775736.1| taurine ABC transporter permeas... 142 4e-35 gi|300310817|ref|YP_003774909.1| nitrate/sulfonate/bicarbonate A... 115 5e-27 >gi|300310979|ref|YP_003775071.1| alkanesulfonates ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 279 Score = 436 bits (1120), Expect = e-123, Method: Compositional matrix adjust. Identities = 253/281 (90%), Positives = 261/281 (92%), Gaps = 2/281 (0%) Query: 1 MTSASDTVLPGAATAAQPSAQPKAAAKKSRPQRDAWIGWILPLLLLVWWEIAAQAGWLSS 60 MTSAS+ V G Q A+PKAA K +P AWIGW+LPLLLLVWWE+AAQAGWLSS Sbjct: 1 MTSASNPV-AGRVAGPQEQAKPKAARKSFQPSH-AWIGWLLPLLLLVWWEVAAQAGWLSS 58 Query: 61 RILPEPFAVLKAAWQLSASGELWHHLAVSSGRAAAGFAVGAGLGLILGLLSGSLRWAELL 120 RILPEP AVLKAAW+LSASGELW HLAVSSGRAA+GFAVGAGLGLILGLLSGSLR AELL Sbjct: 59 RILPEPLAVLKAAWELSASGELWRHLAVSSGRAASGFAVGAGLGLILGLLSGSLRSAELL 118 Query: 121 LDTTLQMVRNIPALALIPLVILWFGIDETAKLFLVSVGVFFPVYLNTFHGIRSVDQGLIE 180 LDTTLQMVRNIPALALIPLVILWFGIDETAKLFLVSVGVFFPVYLNTFHGIRSVDQGLIE Sbjct: 119 LDTTLQMVRNIPALALIPLVILWFGIDETAKLFLVSVGVFFPVYLNTFHGIRSVDQGLIE 178 Query: 181 MAKSYGLSGWPLYRDVILPGALPSILVGLRFALGLVWVLLIVAETISAQAGIGYMTMNAR 240 MAKSYGLSGWPLYRDVILPGALP+ILVGLRFALGLVWVLLIVAETISAQAGIGYMTMNAR Sbjct: 179 MAKSYGLSGWPLYRDVILPGALPAILVGLRFALGLVWVLLIVAETISAQAGIGYMTMNAR 238 Query: 241 EFLQTDVVLVGILLYALLGKLADTAARALERFWLRWHPGYQ 281 EFLQTDVVLVGILLYALLGKLADTAAR LERFWLRWHPGYQ Sbjct: 239 EFLQTDVVLVGILLYALLGKLADTAARWLERFWLRWHPGYQ 279 >gi|300309985|ref|YP_003774077.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 278 Score = 190 bits (483), Expect = 9e-50, Method: Compositional matrix adjust. Identities = 113/248 (45%), Positives = 156/248 (62%), Gaps = 2/248 (0%) Query: 30 RPQRDAWIGWILPLLLLVWWEIAAQAGWLSSRILPEPFAVLKAAWQLSASGELWHHLAVS 89 RP R + GWI+PL L+ E A+ + +R+LP P + W L+ GEL H+A S Sbjct: 22 RPGR--FTGWIVPLAFLLLVEAGARLELIPARLLPPPSELAHTLWSLAQRGELLPHIAAS 79 Query: 90 SGRAAAGFAVGAGLGLILGLLSGSLRWAELLLDTTLQMVRNIPALALIPLVILWFGIDET 149 R AAGF +G+ L + LGLL G R AE LL+ + Q +R IP+LA IPL++LW GIDET Sbjct: 80 LARVAAGFVIGSALAVALGLLVGISRRAEALLEPSFQALRAIPSLAWIPLLLLWLGIDET 139 Query: 150 AKLFLVSVGVFFPVYLNTFHGIRSVDQGLIEMAKSYGLSGWPLYRDVILPGALPSILVGL 209 K+ L+++G FFPVYLN GIR +D LIE+ + GL+ L ++LP +LP + GL Sbjct: 140 PKVVLIAIGAFFPVYLNLVAGIRDIDHKLIEVGTTSGLNTQALIGHILLPASLPYLFAGL 199 Query: 210 RFALGLVWVLLIVAETISAQAGIGYMTMNAREFLQTDVVLVGILLYALLGKLADTAARAL 269 R L L W+ L+ AE I+A G+GY+ + RE + D+VL IL+ ALLGKL+D RA+ Sbjct: 200 RGGLSLAWMFLVAAELIAATKGVGYLLSDGRESSRPDLVLAAILILALLGKLSDGLLRAV 259 Query: 270 ERFWLRWH 277 E+ L W Sbjct: 260 EQKSLGWR 267 >gi|300309716|ref|YP_003773808.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 273 Score = 159 bits (401), Expect = 4e-40, Method: Compositional matrix adjust. Identities = 104/263 (39%), Positives = 153/263 (58%), Gaps = 21/263 (7%) Query: 38 GWILPLLLLVWWEIAAQAGWLSSRILPEPFAVLKA-------------------AWQLSA 78 G ++P++++ WE A++AGW++ +ILP P+AVL+ AW S Sbjct: 12 GLLVPIIVIALWEGASRAGWINPQILPSPWAVLRKWVEYATPLKPYDPEAGSWLAWAFS- 70 Query: 79 SGELWHHLAVSSGRAAAGFAVGAGLGLILGLLSGSLRWAELLLDTTLQMVRNIPALALIP 138 GEL S R AGF +GAGL L LGLL GS + L++ T+Q++R IP +A IP Sbjct: 71 -GELVMDAIGSLYRVVAGFLIGAGLALPLGLLMGSSQRIYGLMNLTVQVIRPIPPIAYIP 129 Query: 139 LVILWFGIDETAKLFLVSVGVFFPVYLNTFHGIRSVDQGLIEMAKSYGLSGWPLYRDVIL 198 L ILWFG+ LFL+S+G FFPV +NT G+R VD + A++ G S L+ V+L Sbjct: 130 LAILWFGLGNPPALFLISLGAFFPVLMNTIAGVRQVDSIYLRAARNLGASQSTLFLRVML 189 Query: 199 PGALPSILVGLRFALGLVWVLLIVAETISAQAGIGYMTMNAREFLQTDVVLVGILLYALL 258 P A+P IL G+R +G ++++IVAE I+ G+G+ M ARE+ +D ++ G++ LL Sbjct: 190 PAAVPYILSGVRIGIGTAFIVVIVAEMIAVSNGLGFRIMEAREYFWSDKIIAGMITIGLL 249 Query: 259 GKLADTAARALERFWLRWHPGYQ 281 G D L + LRWH G + Sbjct: 250 GLAIDLGMSRLNNYMLRWHRGLE 272 >gi|300311644|ref|YP_003775736.1| taurine ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 293 Score = 142 bits (357), Expect = 4e-35, Method: Compositional matrix adjust. Identities = 96/239 (40%), Positives = 134/239 (56%), Gaps = 5/239 (2%) Query: 43 LLLLVWWEIAAQAGWLSSRILPEPFAVLKAAWQL----SASGELWHHLAVSSGRAAAGFA 98 ++L +WW IA Q GW+ LP P VLK A + A+ L H+ S R Sbjct: 53 VILALWWLIA-QTGWVPHLFLPTPAEVLKVAQSIWEDGYANATLAEHVGASLLRILTAGV 111 Query: 99 VGAGLGLILGLLSGSLRWAELLLDTTLQMVRNIPALALIPLVILWFGIDETAKLFLVSVG 158 + LG+ +GLL G RW + +LDT ++ +P LA +PL+I+W GI E +K+ L+++ Sbjct: 112 IAVLLGIPVGLLMGLNRWVKGVLDTPIEFYWPLPPLAYLPLMIIWLGIGEASKITLLTLA 171 Query: 159 VFFPVYLNTFHGIRSVDQGLIEMAKSYGLSGWPLYRDVILPGALPSILVGLRFALGLVWV 218 +F PV L+ G+RS+ Q + A S G + W L +V+LP ALP IL G+R ALG+ W Sbjct: 172 MFAPVVLSAQAGVRSLPQERVNAALSLGATRWQLLTEVVLPSALPEILTGIRIALGVGWS 231 Query: 219 LLIVAETISAQAGIGYMTMNAREFLQTDVVLVGILLYALLGKLADTAARALERFWLRWH 277 L+ AE I++ GIGYM M+A FL TD V VGI L A R LER + W Sbjct: 232 TLVAAELIASNRGIGYMIMSASHFLATDAVFVGIALIATCAFAFSYGMRLLERALVPWK 290 >gi|300310817|ref|YP_003774909.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 256 Score = 115 bits (287), Expect = 5e-27, Method: Compositional matrix adjust. Identities = 79/251 (31%), Positives = 124/251 (49%), Gaps = 2/251 (0%) Query: 21 QPKAAAKKSRPQRDAWIGWILPLLLLVWWEIAAQAGWLSSRILPEPFAVLKAAWQLSASG 80 P ++ + R I I + LV W++ + S LP AA +L + G Sbjct: 1 MPSNLGRRLKAARPFLISVIAIVGGLVLWQLVSLT--TSPLFLPSVSMTWAAALELISDG 58 Query: 81 ELWHHLAVSSGRAAAGFAVGAGLGLILGLLSGSLRWAELLLDTTLQMVRNIPALALIPLV 140 L + SSGR AG+ G +G+ LG+ G LLD ++ R IP +A + L Sbjct: 59 TLQQSVLASSGRILAGWGAGVVIGVPLGIFMGRFETVRQLLDPYIEFFRFIPPIAFVTLA 118 Query: 141 ILWFGIDETAKLFLVSVGVFFPVYLNTFHGIRSVDQGLIEMAKSYGLSGWPLYRDVILPG 200 ++W G E +K+ L+ F V LNT G+++V+ + A S G W + VILP Sbjct: 119 VIWLGPGELSKIALIFYTTVFIVTLNTIAGVQAVNPLRLRAAASLGAGQWKILSTVILPS 178 Query: 201 ALPSILVGLRFALGLVWVLLIVAETISAQAGIGYMTMNAREFLQTDVVLVGILLYALLGK 260 +P ++ G R A+G ++ ++ AE +SA+ G+G + AR F +T+ V VGI+ +LG Sbjct: 179 TVPFMVTGARIAMGNSFLTVVSAEIVSAREGLGALIWTARNFSRTEWVFVGIIALGILGY 238 Query: 261 LADTAARALER 271 L D R R Sbjct: 239 LFDRVLRVATR 249 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.139 0.439 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 745,280 Number of extensions: 27694 Number of successful extensions: 216 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 208 Number of HSP's successfully gapped: 17 Length of query: 281 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 197 Effective length of database: 1,222,130 Effective search space: 240759610 Effective search space used: 240759610 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 130 (54.7 bits)