BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1523.p (327 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310977|ref|YP_003775069.1| alkanesulfonates ABC transporte... 617 e-178 gi|300309984|ref|YP_003774076.1| nitrate/sulfonate/bicarbonate A... 286 2e-78 gi|300310915|ref|YP_003775007.1| alkanesulfonates ABC transporte... 282 3e-77 gi|300313870|ref|YP_003777962.1| nitrate/sulfonate/bicarbonate A... 249 2e-67 gi|300309986|ref|YP_003774078.1| nitrate/sulfonate/bicarbonate A... 139 3e-34 >gi|300310977|ref|YP_003775069.1| alkanesulfonates ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 327 Score = 617 bits (1591), Expect = e-178, Method: Compositional matrix adjust. Identities = 304/327 (92%), Positives = 318/327 (97%) Query: 1 VSANKNKSIHLPRRRSLARLSAIALGALTIGLGASPVQAQETKLLRIGYQKAANTLVLLK 60 +SANKNK++H RRR++ RLSAIALGALTI LGASPVQAQETKLLRIGYQKAANTLVLLK Sbjct: 1 MSANKNKTVHQARRRAIQRLSAIALGALTIALGASPVQAQETKLLRIGYQKAANTLVLLK 60 Query: 61 AHGALEKRLQPLGVEVKWAEFAAGPQLLEALNVGSVDFGYVGEAPPVFAQAAGADFVYTA 120 AHG LEKRLQPLGVEVKWAEFAAGPQLLEALNVGSVDFGYVGEAPPVFAQAAGADFVYTA Sbjct: 61 AHGTLEKRLQPLGVEVKWAEFAAGPQLLEALNVGSVDFGYVGEAPPVFAQAAGADFVYTA 120 Query: 121 YEIPTPEAEALLVPKNSPIKSVAELKGKKVAFNKGSDVHWLVVALLKKAGLQYSDIQPVY 180 YEIPTP+AE LLV KNSPIKSVAELKGKK+AFNKGSDVHWLVVALLKKAGLQYSDIQPVY Sbjct: 121 YEIPTPDAEGLLVQKNSPIKSVAELKGKKIAFNKGSDVHWLVVALLKKAGLQYSDIQPVY 180 Query: 181 LAPADARAAFERGAVDAWAIWDPFQAAAEKQVGARRLASGAGVVSHHQFFLSARPFAAKN 240 LAPADARAAFERGAVDAWAIWDPFQAAAEKQVGARRLA+G G V+HHQFFLSARPFAAKN Sbjct: 181 LAPADARAAFERGAVDAWAIWDPFQAAAEKQVGARRLATGVGAVNHHQFFLSARPFAAKN 240 Query: 241 PQVQKILLEEITREGEWVRTHTAEAAAQLSPIQGLDADIIETGLKRYAHIYKPVDAQVLA 300 PQV+KILLEEI+REGEWVRTHTA+AAAQL+PIQGLDADIIETGLKRYAH+YKPVDAQVLA Sbjct: 241 PQVEKILLEEISREGEWVRTHTAQAAAQLAPIQGLDADIIETGLKRYAHVYKPVDAQVLA 300 Query: 301 EQQKIADAFYELKLIPKPLKVSDAVLK 327 EQQKIADAFYELKLIPKPLKVSDAVLK Sbjct: 301 EQQKIADAFYELKLIPKPLKVSDAVLK 327 >gi|300309984|ref|YP_003774076.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 328 Score = 286 bits (731), Expect = 2e-78, Method: Compositional matrix adjust. Identities = 145/320 (45%), Positives = 208/320 (65%), Gaps = 4/320 (1%) Query: 8 SIHLPRRRSLARLSAIALGALTIGLGASPVQAQETKLLRIGYQKAANTLVLLKAHGALEK 67 S RRR L +A+ LG+ L + V AQ K +RIGYQK++ L +LKA+G LEK Sbjct: 5 STDSTRRRLLHAATALGLGS---ALAPASVFAQGNKAIRIGYQKSSTLLTILKANGTLEK 61 Query: 68 RLQPLGVEVKWAEFAAGPQLLEALNVGSVDFGY-VGEAPPVFAQAAGADFVYTAYEIPTP 126 L P G ++ W EFA+G LLEALNVG VD V + PVFAQAAGA Y A E P+P Sbjct: 62 LLAPTGAKISWHEFASGLPLLEALNVGGVDLSADVADTVPVFAQAAGAKLAYVAQESPSP 121 Query: 127 EAEALLVPKNSPIKSVAELKGKKVAFNKGSDVHWLVVALLKKAGLQYSDIQPVYLAPADA 186 A+A++V +S ++ ++LKGKK+A K + VH+L++A L+KAGL++SD+ YL+PAD Sbjct: 122 AAQAIVVRADSVLRKASDLKGKKIAVTKAAGVHYLLIATLEKAGLKFSDVDAAYLSPADG 181 Query: 187 RAAFERGAVDAWAIWDPFQAAAEKQVGARRLASGAGVVSHHQFFLSARPFAAKNPQVQKI 246 RAAFERG+VDAW WDPF A ++Q R L+ G GV + +++L++ FA P+V ++ Sbjct: 182 RAAFERGSVDAWVTWDPFLAGVQRQSQVRILSDGRGVADYQRYYLASASFAQARPEVLRV 241 Query: 247 LLEEITREGEWVRTHTAEAAAQLSPIQGLDADIIETGLKRYAHIYKPVDAQVLAEQQKIA 306 + +E+ + G WV+ H EAAA L+P+ GLDAD IE R ++ + V + L EQQ+IA Sbjct: 242 VFDELQKTGHWVKQHPKEAAAFLAPLWGLDADTIELANSRRSYEVRAVRPEALGEQQRIA 301 Query: 307 DAFYELKLIPKPLKVSDAVL 326 D F+ L+P+ + +D + Sbjct: 302 DTFFAAGLLPRKINTNDVAI 321 >gi|300310915|ref|YP_003775007.1| alkanesulfonates ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 336 Score = 282 bits (721), Expect = 3e-77, Method: Compositional matrix adjust. Identities = 153/327 (46%), Positives = 213/327 (65%), Gaps = 7/327 (2%) Query: 3 ANKNKSIHL----PRRRSLARLSAIALGALTIGLG-ASPVQAQETKLLRIGYQKAANTLV 57 A +NK+I PRRR L + A G + V A + LR+GYQK A L Sbjct: 6 AQRNKAIQTAVRSPRRRRLIAGAVTLGAAAAAGASLPAWVHAAAPRQLRVGYQKGA--LS 63 Query: 58 LLKAHGALEKRLQPLGVEVKWAEFAAGPQLLEALNVGSVDFGYVGEAPPVFAQAAGADFV 117 L++AHG LE+RL PLG VKW EF+AGP LEAL GS+DFG VGEAPP+FAQAAG V Sbjct: 64 LIRAHGVLEQRLAPLGATVKWVEFSAGPVQLEALGAGSIDFGDVGEAPPIFAQAAGTPLV 123 Query: 118 YTAYEIPTPEAEALLVPKNSPIKSVAELKGKKVAFNKGSDVHWLVVALLKKAGLQYSDIQ 177 Y +P P EA+LV +S ++ +LKGK++A NKGS+VH+ +V LL+K GL+Y D+ Sbjct: 124 YVGATVPRPYVEAVLVKDDSELQRPQQLKGKRIALNKGSNVHYFLVKLLEKHGLKYGDVT 183 Query: 178 PVYLAPADARAAFERGAVDAWAIWDPFQAAAEKQVGARRLASGAGVVSHHQFFLSARPFA 237 PV+LAPADARAAFERGAVDAWAIW+P+ AAA++ + AR LA GVV + ++ S R +A Sbjct: 184 PVWLAPADARAAFERGAVDAWAIWEPYLAAAQQGLKARILADADGVVKNRAYYFSTRDYA 243 Query: 238 AKNPQVQKILLEEITREGEWVRTHTAEAAAQLSPIQGLDADIIETGLKRYAHIYKPVDAQ 297 + + +I LEE+ + W + E A++L+ I GL +++ ++RY + P+ Sbjct: 244 RDHADLLRIALEELGKIDAWAQARPDEYASELAGILGLPRSVLDVVVRRYHYGTLPITRA 303 Query: 298 VLAEQQKIADAFYELKLIPKPLKVSDA 324 +L EQQ+IAD F +L+LIP+ + + +A Sbjct: 304 ILDEQQQIADTFLDLRLIPRKISLLEA 330 >gi|300313870|ref|YP_003777962.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 323 Score = 249 bits (637), Expect = 2e-67, Method: Compositional matrix adjust. Identities = 138/318 (43%), Positives = 193/318 (60%), Gaps = 6/318 (1%) Query: 10 HLPRRRSLARLSAIALGALTIGLGASP-VQAQETKLLRIGYQKAANTLVLLKAHGALEKR 68 H PR + L +A GAL G +P V+A K+LRIGYQK NTL +LK G LE+ Sbjct: 4 HQPRHKRRLLLQGLAAGALASG---TPLVRAAADKVLRIGYQKF-NTLNILKGSGKLEQA 59 Query: 69 LQPLGVEVKWAEFAAGPQLLEALNVGSVDFGYVGEAPPVFAQAAGADFVYTAYEIPTPEA 128 LQP G VKW EFAAGPQL EALN ++D G+ + P VFA A+G + VY E P P+ Sbjct: 60 LQPQGWSVKWFEFAAGPQLTEALNANAIDLGHAADTPSVFANASGVNAVYLGAEQPYPKG 119 Query: 129 EALLVPKNSPIKSVAELKGKKVAFNKGSDVHWLVVALLKKAGLQYSDIQPVYLA-PADAR 187 + V + SPI+SVA+LKGKKVA +G +V +L++ L++ GL Y DI+PVY+ AD R Sbjct: 120 IGIFVAQESPIRSVADLKGKKVATGRGWNVQFLLLKALEEVGLSYRDIEPVYVTNAADVR 179 Query: 188 AAFERGAVDAWAIWDPFQAAAEKQVGARRLASGAGVVSHHQFFLSARPFAAKNPQVQKIL 247 AAF+ G VDA +WDPF A E R L G G+ ++ F L+ F ++ + + + Sbjct: 180 AAFQSGNVDAATLWDPFLAGQELATRPRILRDGTGLSNNRTFHLAVPSFVDRHKDIVRTV 239 Query: 248 LEEITREGEWVRTHTAEAAAQLSPIQGLDADIIETGLKRYAHIYKPVDAQVLAEQQKIAD 307 E+ + +W ++H E A L+P G+D ++ +R + VDA ++AEQQ+IAD Sbjct: 240 FAELKKTNQWTQSHPQETAELLAPQLGVDPKVLRLATERRNYTTVAVDAGIVAEQQQIAD 299 Query: 308 AFYELKLIPKPLKVSDAV 325 AF +L LI P++V D V Sbjct: 300 AFLKLGLIKNPVRVQDKV 317 >gi|300309986|ref|YP_003774078.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 328 Score = 139 bits (350), Expect = 3e-34, Method: Compositional matrix adjust. Identities = 102/276 (36%), Positives = 143/276 (51%), Gaps = 7/276 (2%) Query: 14 RRSLARLSAIALGALTIGLGASPV---QAQETKLLRIGYQKAANTLVLLKAHGALEKRLQ 70 RR RL+ ALG LG P+ AQ K++R+ Y + ++L+ G LE+ L+ Sbjct: 3 RRDFLRLAPAALG--VTALGGIPLLSSAAQPPKVIRLDYAYYSPPSLVLRKFGWLEEDLK 60 Query: 71 PLGVEVKWAEFAAGPQLLEALNVGSVDFGYVGEAPPVFAQAAGADFVYTAYEIPTPEAEA 130 G EVKW + LE LN SVDFG + A+A G + V Y PE A Sbjct: 61 AQGTEVKWVLSQGSNRALEYLNSDSVDFGSTAGLAALLARANG-NPVKAVYIYSRPEWTA 119 Query: 131 LLVPKNSPIKSVAELKGKKVAFNKGSDVHWLVVALLKKAGLQYSDIQPVYLAPADARAAF 190 L V K SP+KS+ ELKGKK+A KG+D + ++ L + GL+ SD++ V+L AD RAA Sbjct: 120 LAVGKESPVKSINELKGKKIAATKGTDPYLFLLRALHENGLKKSDVEIVHLQHADGRAAL 179 Query: 191 ERGAVDAWAIWDPFQAAAEKQVGARRLASGAGVVSHHQFFLSARPFAAKNPQVQKILLEE 250 E+G V AWA DP AA+E + G+R + + + F + FAA++P K ++ Sbjct: 180 EQGRVAAWAGLDPHLAASELEAGSRLIYRNVN-FNTYGFLNVSDSFAARHPDQVKRVIAA 238 Query: 251 ITREGEWVRTHTAEAAAQLSPIQGLDADIIETGLKR 286 R W+ H E A LS L A + LKR Sbjct: 239 YERARSWIIAHPDETVALLSEESKLSAPVARLQLKR 274 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.133 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 974,215 Number of extensions: 40870 Number of successful extensions: 178 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 170 Number of HSP's successfully gapped: 8 Length of query: 327 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 242 Effective length of database: 1,217,395 Effective search space: 294609590 Effective search space used: 294609590 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)