BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1477.p (174 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310920|ref|YP_003775012.1| integral membrane protein [Herb... 287 4e-79 >gi|300310920|ref|YP_003775012.1| integral membrane protein [Herbaspirillum seropedicae SmR1] Length = 178 Score = 287 bits (734), Expect = 4e-79, Method: Compositional matrix adjust. Identities = 159/178 (89%), Positives = 164/178 (92%), Gaps = 4/178 (2%) Query: 1 MLRSESLFGKRETNSTNSPFSSAGSSVS--SSTGGNVTSVNKAAPYPSSVS--HAAPAAT 56 MLRSESLFGKRET STNSPFSSA S+ S + TGGNVTSVNK A YPSSVS A AA Sbjct: 1 MLRSESLFGKRETTSTNSPFSSASSNGSTSTPTGGNVTSVNKPAQYPSSVSTPAPAQAAA 60 Query: 57 EESGSKLTVGPNIKVKGEIEDCDTLVVEGKVQATINCRVVQIAERGAFKGSAEIDLAEIR 116 EESGSKLTVGPNIKVKGEIEDCDTLVVEGKVQATINCRVVQIAERGAFKGSAEIDLAEIR Sbjct: 61 EESGSKLTVGPNIKVKGEIEDCDTLVVEGKVQATINCRVVQIAERGAFKGSAEIDLAEIR 120 Query: 117 GEFDGDLTVRQKLVIYSSGKVTGKIRYGKLVIEEGGQLAGDLQVGGGHEQKDLLQKTA 174 GEFDG+LTVRQKLVIY+SGKVTGKIRYGKLVIEEGGQLAGDLQVGGGHEQK+LLQKTA Sbjct: 121 GEFDGELTVRQKLVIYASGKVTGKIRYGKLVIEEGGQLAGDLQVGGGHEQKELLQKTA 178 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.308 0.128 0.345 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 447,835 Number of extensions: 16518 Number of successful extensions: 55 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 53 Number of HSP's successfully gapped: 1 Length of query: 174 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 95 Effective length of database: 1,245,805 Effective search space: 118351475 Effective search space used: 118351475 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.6 bits) S2: 127 (53.5 bits)