BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1475.p (570 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310916|ref|YP_003775008.1| phospholipase C protein [Herbas... 1038 0.0 gi|300311919|ref|YP_003776011.1| non-hemolytic phospholipase C (... 82 1e-16 >gi|300310916|ref|YP_003775008.1| phospholipase C protein [Herbaspirillum seropedicae SmR1] Length = 558 Score = 1038 bits (2684), Expect = 0.0, Method: Compositional matrix adjust. Identities = 510/555 (91%), Positives = 527/555 (94%), Gaps = 3/555 (0%) Query: 19 PILPQ---NNSRRRFLGGMAALGAGSALAACATEGQGNTAGPGGNPLEAAALDRALRENV 75 PILP N + LGGMAALGAGSALA CATEGQG+ P GNP EAAALDRALRE V Sbjct: 4 PILPSLCSNPAAAASLGGMAALGAGSALAGCATEGQGSAPPPSGNPAEAAALDRALREQV 63 Query: 76 KTIVVIYAENRSFNNLFADFPGLEKPLSSLKPADYQQRDRDGSLLKELPPAWNGVLIQDQ 135 KTIVVIYAENRSFNNLFADFPGLEKPLS+LKP+DYQQRDRDGSLLK+LPPAW+GV+ Q+Q Sbjct: 64 KTIVVIYAENRSFNNLFADFPGLEKPLSALKPSDYQQRDRDGSLLKQLPPAWSGVVNQEQ 123 Query: 136 SVEGVTYAGGKQYQTNLPNAPYALKGPQGEALPLSLVTRDLWHVFYQNQMQINGGKNDGF 195 +VEGVTY GKQYQTNLPNAPYALKGPQGEALPL LVTRDLWHVFYQNQMQINGGKNDGF Sbjct: 124 TVEGVTYEAGKQYQTNLPNAPYALKGPQGEALPLGLVTRDLWHVFYQNQMQINGGKNDGF 183 Query: 196 VAWADSGGLTMGHYGDTKYNLRLWDVAREFVLCDNFFQGAFGGSFLNHQYLICATAPFYP 255 VAWADSGGLTMGHY DT+Y+LRLWDVAREFVLCDNFFQGAFGGSFLNHQYLICATAP+YP Sbjct: 184 VAWADSGGLTMGHYADTRYHLRLWDVAREFVLCDNFFQGAFGGSFLNHQYLICATAPYYP 243 Query: 256 NVMSSVSRTQVAKTRNNDPLSPELVPEEGSPASALQGPPKFGPSALTPDGYAVNTMAPPY 315 NVM+SVSRTQVAKT+NNDPLSPELVP EGSP SALQGPPKFGPSALTPDGYAVNTMAPPY Sbjct: 244 NVMNSVSRTQVAKTKNNDPLSPELVPAEGSPTSALQGPPKFGPSALTPDGYAVNTMAPPY 303 Query: 316 WPTWSRDRNNPAYAEPNQPSVLVPQTHEHIGDKLNKKGIDWAWYAGAWQATLDEFKDSKG 375 WPTWSRDRNNPAYAEPNQPSVLVPQTHEHIGDKLNKKGIDWAWYAGAWQATLDEFKDSKG Sbjct: 304 WPTWSRDRNNPAYAEPNQPSVLVPQTHEHIGDKLNKKGIDWAWYAGAWQATLDEFKDSKG 363 Query: 376 IPKIPNFQYHHQPFNYFKNLGPENPAERNKRLRDGGLGDEARTNRFLADVEAGRLPPVTF 435 IPKIPNFQYHHQPFNYFKNLGPENPAERNKRLRDGGLGDEARTNRFLADVEAGRLPPVTF Sbjct: 364 IPKIPNFQYHHQPFNYFKNLGPENPAERNKRLRDGGLGDEARTNRFLADVEAGRLPPVTF 423 Query: 436 YKPQGNLNLHAGYADIASGDRHIAHVIKSLQASPQWKNMVVVITFDENGGWWDHVAPPKG 495 YKPQGNLNLHAGYADIASGDRHIAH+IKSLQASPQWKNMVVV+TFDENGGWWDHVAPPKG Sbjct: 424 YKPQGNLNLHAGYADIASGDRHIAHIIKSLQASPQWKNMVVVVTFDENGGWWDHVAPPKG 483 Query: 496 DRWGPGTRVPALVVSPFARKGMVDHTIYDTASILRLITRTFGLETLDGLKQRDEAMIARG 555 DRWGPGTRVPALVVSPFARKG VDHT+YDTASILRLITRTF LE LDGLKQRDEAMIARG Sbjct: 484 DRWGPGTRVPALVVSPFARKGTVDHTVYDTASILRLITRTFDLEKLDGLKQRDEAMIARG 543 Query: 556 QQPMGDLTNALQFKA 570 QQPMGDLTNALQ K Sbjct: 544 QQPMGDLTNALQLKV 558 >gi|300311919|ref|YP_003776011.1| non-hemolytic phospholipase C (phosphatidylcholine choline phospho hydrolase) signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 696 Score = 82.4 bits (202), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 120/536 (22%), Positives = 193/536 (36%), Gaps = 161/536 (30%) Query: 73 ENVKTIVVIYAENRSFNNLFADFPGLE-------KPLSSLKPADYQQRDRDGSLLKELPP 125 ++VK +V++ ENRSF++ F G+ +PL P +Q RDG Sbjct: 48 KDVKHVVILMQENRSFDHYFGCLRGVRGFADPIPRPLPDGSPVFHQ---RDG-------- 96 Query: 126 AWNGVLIQDQSVEGVTYAGGKQYQTNLPNAPYALKGPQGEALPLSLVTRDLWHVFYQNQM 185 G L+ +E T + G+ NLP H + Sbjct: 97 --QGRLVLPYRMEMATTSAGRA-GGNLP------------------------HAWKDQHA 129 Query: 186 QINGGKNDGFVAWADSGGLTMGHYGDTKYNLRL-WDVAREFVLCDNFFQGAFGGSFLNHQ 244 N G D ++ A +TM ++ T+ +L + +A F LCD + G + N Sbjct: 130 AWNRGDYDKWIE-AKKDTVTMAYF--TREDLPFHYALADAFTLCDAYHCSVLGSTNPNRF 186 Query: 245 YLICATAPFYPNVMSSVSRTQVAKTRNNDPLS--PELVPEEGSPASALQGPPKFGPSALT 302 +L+ + R P++ P G P G FG Sbjct: 187 HLMSGGIDL--------------QGRAGGPMTFQPRTGSCSGVPGERRSGADSFG----- 227 Query: 303 PDGYAVNTMAPPYWPTWSRDRNNPAYAEPNQPSVLVPQTHEHIGDKLNKKGIDWAWYAGA 362 W T+ ++L + G+ W Y Sbjct: 228 -------------WTTYP--------------------------ERLEQHGVSWKVY--- 245 Query: 363 WQATLDEFKDSKGIPKIPNFQYHHQPFNYFKNLGPENPAERNKRLRDGGLGDEARTNRFL 422 Q T + KD P +F F ++N P++P + K L D AR Sbjct: 246 -QGTEELHKDHGDYPS--DFNVLQYFFAQYQNAAPDSPLWQ-KACSKFTLADLAR----- 296 Query: 423 ADVEAGRLPPVTFYKPQGNLNLHAGYADIASGDRHIAHVIKSLQASPQ-WKNMVVVITFD 481 DV +G+LP V++ P L+ G +I+ V+ L A+P W + V+++ +D Sbjct: 297 -DVNSGKLPQVSWLMPP-KLSCEHPDRTPGYGAHYISQVLDILTANPALWSSTVLLVNYD 354 Query: 482 ENGGWWDHVAPP---------------------KGDRWGP--------GTRVPALVVSPF 512 EN G++DHV PP KGD P G RVP +V+SP+ Sbjct: 355 ENDGFFDHVVPPTPPLHAGQGLSTVETSAEFHRKGDYVNPADDLPAGLGARVPMMVISPW 414 Query: 513 ARKGMVDHTIYDTASILRLITRTFGLETLDGLKQRDEAMIARGQQPMGDLTNALQF 568 ++ G V ++D S++R + + F + + R GDLT+A F Sbjct: 415 SKGGYVCSEVFDHTSVIRFLEQVFDVHEPNISPWRRAV--------CGDLTSAFDF 462 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.135 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,973,332 Number of extensions: 88223 Number of successful extensions: 212 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 213 Number of HSP's successfully gapped: 2 Length of query: 570 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 480 Effective length of database: 1,193,720 Effective search space: 572985600 Effective search space used: 572985600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)