BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1473.p (334 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310915|ref|YP_003775007.1| alkanesulfonates ABC transporte... 538 e-154 gi|300310977|ref|YP_003775069.1| alkanesulfonates ABC transporte... 287 9e-79 gi|300309984|ref|YP_003774076.1| nitrate/sulfonate/bicarbonate A... 249 2e-67 gi|300313870|ref|YP_003777962.1| nitrate/sulfonate/bicarbonate A... 223 2e-59 gi|300309986|ref|YP_003774078.1| nitrate/sulfonate/bicarbonate A... 94 2e-20 >gi|300310915|ref|YP_003775007.1| alkanesulfonates ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 336 Score = 538 bits (1386), Expect = e-154, Method: Compositional matrix adjust. Identities = 277/336 (82%), Positives = 303/336 (90%), Gaps = 2/336 (0%) Query: 1 MPQQEDQRSRAIAVVEHNSKRRRLLATGLALGTAATLATLAPRPARAAS-RQLRVGYQKG 59 MP + QR++AI + +RRRL+A + LG AA P AA+ RQLRVGYQKG Sbjct: 1 MPHDQAQRNKAIQTAVRSPRRRRLIAGAVTLGAAAAAGASLPAWVHAAAPRQLRVGYQKG 60 Query: 60 ALSIIRAHGTLEQRLAPLGASVKWVEFSAGPVQLEALGAGSIDFGDVGEAPPIFAQAAGT 119 ALS+IRAHG LEQRLAPLGA+VKWVEFSAGPVQLEALGAGSIDFGDVGEAPPIFAQAAGT Sbjct: 61 ALSLIRAHGVLEQRLAPLGATVKWVEFSAGPVQLEALGAGSIDFGDVGEAPPIFAQAAGT 120 Query: 120 PLVYVGATVPRPYVEAVLVKDDS-LLKPEQLKGKRIALNKGSNVHYFLVKLLQKHGLQYG 178 PLVYVGATVPRPYVEAVLVKDDS L +P+QLKGKRIALNKGSNVHYFLVKLL+KHGL+YG Sbjct: 121 PLVYVGATVPRPYVEAVLVKDDSELQRPQQLKGKRIALNKGSNVHYFLVKLLEKHGLKYG 180 Query: 179 DVTPVWLAPADARAAFERGSVDAWAIWEPYLAAAQQTLKARILADADGVVKNRAYYFSTR 238 DVTPVWLAPADARAAFERG+VDAWAIWEPYLAAAQQ LKARILADADGVVKNRAYYFSTR Sbjct: 181 DVTPVWLAPADARAAFERGAVDAWAIWEPYLAAAQQGLKARILADADGVVKNRAYYFSTR 240 Query: 239 DYARDNADLIRIALEELAKVDEFAKTRPDDYANELAGILGLPKSVLDVVVRRYHYGTLPI 298 DYARD+ADL+RIALEEL K+D +A+ RPD+YA+ELAGILGLP+SVLDVVVRRYHYGTLPI Sbjct: 241 DYARDHADLLRIALEELGKIDAWAQARPDEYASELAGILGLPRSVLDVVVRRYHYGTLPI 300 Query: 299 TQAILDEQQQIADTFLDLKLIPRKINLRETAPPWLA 334 T+AILDEQQQIADTFLDL+LIPRKI+L E APPWLA Sbjct: 301 TRAILDEQQQIADTFLDLRLIPRKISLLEAAPPWLA 336 >gi|300310977|ref|YP_003775069.1| alkanesulfonates ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 327 Score = 287 bits (734), Expect = 9e-79, Method: Compositional matrix adjust. Identities = 154/316 (48%), Positives = 216/316 (68%), Gaps = 7/316 (2%) Query: 17 HNSKRRRLL-ATGLALGTAATLATLAPRPARA-ASRQLRVGYQKGA--LSIIRAHGTLEQ 72 H ++RR + + +ALG A T+A L P +A ++ LR+GYQK A L +++AHGTLE+ Sbjct: 10 HQARRRAIQRLSAIALG-ALTIA-LGASPVQAQETKLLRIGYQKAANTLVLLKAHGTLEK 67 Query: 73 RLAPLGASVKWVEFSAGPVQLEALGAGSIDFGDVGEAPPIFAQAAGTPLVYVGATVPRPY 132 RL PLG VKW EF+AGP LEAL GS+DFG VGEAPP+FAQAAG VY +P P Sbjct: 68 RLQPLGVEVKWAEFAAGPQLLEALNVGSVDFGYVGEAPPVFAQAAGADFVYTAYEIPTPD 127 Query: 133 VEAVLVKDDSLLKP-EQLKGKRIALNKGSNVHYFLVKLLQKHGLQYGDVTPVWLAPADAR 191 E +LV+ +S +K +LKGK+IA NKGS+VH+ +V LL+K GLQY D+ PV+LAPADAR Sbjct: 128 AEGLLVQKNSPIKSVAELKGKKIAFNKGSDVHWLVVALLKKAGLQYSDIQPVYLAPADAR 187 Query: 192 AAFERGSVDAWAIWEPYLAAAQQTLKARILADADGVVKNRAYYFSTRDYARDNADLIRIA 251 AAFERG+VDAWAIW+P+ AAA++ + AR LA G V + ++ S R +A N + +I Sbjct: 188 AAFERGAVDAWAIWDPFQAAAEKQVGARRLATGVGAVNHHQFFLSARPFAAKNPQVEKIL 247 Query: 252 LEELAKVDEFAKTRPDDYANELAGILGLPKSVLDVVVRRYHYGTLPITQAILDEQQQIAD 311 LEE+++ E+ +T A +LA I GL +++ ++RY + P+ +L EQQ+IAD Sbjct: 248 LEEISREGEWVRTHTAQAAAQLAPIQGLDADIIETGLKRYAHVYKPVDAQVLAEQQKIAD 307 Query: 312 TFLDLKLIPRKINLRE 327 F +LKLIP+ + + + Sbjct: 308 AFYELKLIPKPLKVSD 323 >gi|300309984|ref|YP_003774076.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 328 Score = 249 bits (637), Expect = 2e-67, Method: Compositional matrix adjust. Identities = 139/319 (43%), Positives = 196/319 (61%), Gaps = 12/319 (3%) Query: 18 NSKRRRLL--ATGLALGTAATLATLAPRPARA-ASRQLRVGYQKGA--LSIIRAHGTLEQ 72 +S RRRLL AT L LG+A LAP A ++ +R+GYQK + L+I++A+GTLE+ Sbjct: 7 DSTRRRLLHAATALGLGSA-----LAPASVFAQGNKAIRIGYQKSSTLLTILKANGTLEK 61 Query: 73 RLAPLGASVKWVEFSAGPVQLEALGAGSIDFG-DVGEAPPIFAQAAGTPLVYVGATVPRP 131 LAP GA + W EF++G LEAL G +D DV + P+FAQAAG L YV P P Sbjct: 62 LLAPTGAKISWHEFASGLPLLEALNVGGVDLSADVADTVPVFAQAAGAKLAYVAQESPSP 121 Query: 132 YVEAVLVKDDSLL-KPEQLKGKRIALNKGSNVHYFLVKLLQKHGLQYGDVTPVWLAPADA 190 +A++V+ DS+L K LKGK+IA+ K + VHY L+ L+K GL++ DV +L+PAD Sbjct: 122 AAQAIVVRADSVLRKASDLKGKKIAVTKAAGVHYLLIATLEKAGLKFSDVDAAYLSPADG 181 Query: 191 RAAFERGSVDAWAIWEPYLAAAQQTLKARILADADGVVKNRAYYFSTRDYARDNADLIRI 250 RAAFERGSVDAW W+P+LA Q+ + RIL+D GV + YY ++ +A+ +++R+ Sbjct: 182 RAAFERGSVDAWVTWDPFLAGVQRQSQVRILSDGRGVADYQRYYLASASFAQARPEVLRV 241 Query: 251 ALEELAKVDEFAKTRPDDYANELAGILGLPKSVLDVVVRRYHYGTLPITQAILDEQQQIA 310 +EL K + K P + A LA + GL +++ R Y + L EQQ+IA Sbjct: 242 VFDELQKTGHWVKQHPKEAAAFLAPLWGLDADTIELANSRRSYEVRAVRPEALGEQQRIA 301 Query: 311 DTFLDLKLIPRKINLRETA 329 DTF L+PRKIN + A Sbjct: 302 DTFFAAGLLPRKINTNDVA 320 >gi|300313870|ref|YP_003777962.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 323 Score = 223 bits (568), Expect = 2e-59, Method: Compositional matrix adjust. Identities = 125/314 (39%), Positives = 183/314 (58%), Gaps = 6/314 (1%) Query: 21 RRRLLATGLALGTAATLATLAPRPARAASRQLRVGYQK-GALSIIRAHGTLEQRLAPLGA 79 +RRLL GLA G LA+ P AA + LR+GYQK L+I++ G LEQ L P G Sbjct: 9 KRRLLLQGLAAGA---LASGTPLVRAAADKVLRIGYQKFNTLNILKGSGKLEQALQPQGW 65 Query: 80 SVKWVEFSAGPVQLEALGAGSIDFGDVGEAPPIFAQAAGTPLVYVGATVPRPYVEAVLVK 139 SVKW EF+AGP EAL A +ID G + P +FA A+G VY+GA P P + V Sbjct: 66 SVKWFEFAAGPQLTEALNANAIDLGHAADTPSVFANASGVNAVYLGAEQPYPKGIGIFVA 125 Query: 140 DDSLLKP-EQLKGKRIALNKGSNVHYFLVKLLQKHGLQYGDVTPVWLA-PADARAAFERG 197 +S ++ LKGK++A +G NV + L+K L++ GL Y D+ PV++ AD RAAF+ G Sbjct: 126 QESPIRSVADLKGKKVATGRGWNVQFLLLKALEEVGLSYRDIEPVYVTNAADVRAAFQSG 185 Query: 198 SVDAWAIWEPYLAAAQQTLKARILADADGVVKNRAYYFSTRDYARDNADLIRIALEELAK 257 +VDA +W+P+LA + + RIL D G+ NR ++ + + + D++R EL K Sbjct: 186 NVDAATLWDPFLAGQELATRPRILRDGTGLSNNRTFHLAVPSFVDRHKDIVRTVFAELKK 245 Query: 258 VDEFAKTRPDDYANELAGILGLPKSVLDVVVRRYHYGTLPITQAILDEQQQIADTFLDLK 317 +++ ++ P + A LA LG+ VL + R +Y T+ + I+ EQQQIAD FL L Sbjct: 246 TNQWTQSHPQETAELLAPQLGVDPKVLRLATERRNYTTVAVDAGIVAEQQQIADAFLKLG 305 Query: 318 LIPRKINLRETAPP 331 LI + +++ P Sbjct: 306 LIKNPVRVQDKVYP 319 >gi|300309986|ref|YP_003774078.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 328 Score = 93.6 bits (231), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 81/272 (29%), Positives = 133/272 (48%), Gaps = 15/272 (5%) Query: 63 IIRAHGTLEQRLAPLGASVKWVEFSAGPVQLEALGAGSIDFGDVGEAPPIFAQAAGTPL- 121 ++R G LE+ L G VKWV LE L + S+DFG + A+A G P+ Sbjct: 48 VLRKFGWLEEDLKAQGTEVKWVLSQGSNRALEYLNSDSVDFGSTAGLAALLARANGNPVK 107 Query: 122 -VYVGATVPRPYVEAVLVKDDSLLKP-EQLKGKRIALNKGSNVHYFLVKLLQKHGLQYGD 179 VY+ + RP A+ V +S +K +LKGK+IA KG++ + FL++ L ++GL+ D Sbjct: 108 AVYIYS---RPEWTALAVGKESPVKSINELKGKKIAATKGTDPYLFLLRALHENGLKKSD 164 Query: 180 VTPVWLAPADARAAFERGSVDAWAIWEPYLAAAQQTLKARILADADGVVKNRAYYFSTRD 239 V V L AD RAA E+G V AWA +P+LAA++ +R++ V N + + D Sbjct: 165 VEIVHLQHADGRAALEQGRVAAWAGLDPHLAASELEAGSRLI--YRNVNFNTYGFLNVSD 222 Query: 240 -YARDNADLIRIALEELAKVDEFAKTRPDDYANELAGILGLPKSVLDVVVRRYHYGTLPI 298 +A + D ++ + + + PD+ L+ L V + ++R + I Sbjct: 223 SFAARHPDQVKRVIAAYERARSWIIAHPDETVALLSEESKLSAPVARLQLKRNDFSQPQI 282 Query: 299 TQAILDEQQQIADTFLDLKLI------PRKIN 324 + ++ + A L+ L+ PR IN Sbjct: 283 GREHIEALRAAAPILLEEDLVKSGTDLPRTIN 314 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,004,924 Number of extensions: 42260 Number of successful extensions: 174 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 165 Number of HSP's successfully gapped: 7 Length of query: 334 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 248 Effective length of database: 1,212,660 Effective search space: 300739680 Effective search space used: 300739680 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)