BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1472.p (571 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310913|ref|YP_003775005.1| chloride channel EriC protein [... 853 0.0 gi|300311435|ref|YP_003775527.1| chloride channel protein [Herba... 104 2e-23 gi|300311782|ref|YP_003775874.1| chloride channel protein [Herba... 62 2e-10 >gi|300310913|ref|YP_003775005.1| chloride channel EriC protein [Herbaspirillum seropedicae SmR1] Length = 572 Score = 853 bits (2205), Expect = 0.0, Method: Compositional matrix adjust. Identities = 485/572 (84%), Positives = 521/572 (91%), Gaps = 1/572 (0%) Query: 1 MKHKRDYTADRRLPLLCAIAVVIGVLSTGAAWLLIKLIALFTNLFYFQRLSFAASSPASH 60 MKHKRDYTADRRLP LCAIA+ IG+LSTGAAWLL+KLIALFTNLFYFQRLS AAS+PA+H Sbjct: 1 MKHKRDYTADRRLPYLCAIALPIGLLSTGAAWLLLKLIALFTNLFYFQRLSLAASTPATH 60 Query: 61 TLGWIAAVLPVAGGLIVGLMARYGSDKIRGHGIPEAIEAILFGKSRMSPKVALLKPLSSG 120 TLGWIAA+LP+AGGLIVGLMARYGSDKIRGHGIPEAIEAILFGKSRMSPKVALLKPLSSG Sbjct: 61 TLGWIAALLPIAGGLIVGLMARYGSDKIRGHGIPEAIEAILFGKSRMSPKVALLKPLSSG 120 Query: 121 IVIGSGGPFGAEGPIIMTGGSLGSLLAQWLHLTAAERKTLLVSGACAGMTAIFGTPLAAV 180 IVIGSGGPFGAEGPIIMTGGS+GSLLAQ LHLTAAERKTLLV+GACAGMTAIFGTPLAAV Sbjct: 121 IVIGSGGPFGAEGPIIMTGGSIGSLLAQLLHLTAAERKTLLVAGACAGMTAIFGTPLAAV 180 Query: 181 LLAVELLLFELRPRSLLPVALACAVAGFLRPWMIDAGVLFPLQVAPVAPLALLSCLLAGL 240 LLAVELLLFEL+PRSLLPVALACAVAGF+RPW+++AG LFPL VA P+ALLSCL+AGL Sbjct: 181 LLAVELLLFELKPRSLLPVALACAVAGFVRPWLVEAGPLFPLDVAAPTPVALLSCLVAGL 240 Query: 241 ASGVLAALLTLALYRIEDGFHRLPLHWMWWPALGGIAVGLGGLLQPRALGVGYDVIADLL 300 ASGVLAA LTLALYRIEDGFHRL LHWMWWPALGGIAVGLGGLLQPRALGVGYDVI DLL Sbjct: 241 ASGVLAAALTLALYRIEDGFHRLRLHWMWWPALGGIAVGLGGLLQPRALGVGYDVIGDLL 300 Query: 301 NQHMLVSAALVLLMVKAVIWLLALGSGTSGGVLAPLLMMGAGLGLVLSPWLPGGSPTLWP 360 +QHML+SAAL LL+VKAVIWLLALGSGTSGGVLAPLLM+GAGLGLVLSPWLPGGS LWP Sbjct: 301 HQHMLLSAALALLLVKAVIWLLALGSGTSGGVLAPLLMLGAGLGLVLSPWLPGGSDGLWP 360 Query: 361 LVCMAGVLGSVLGAPLTAIVFAFGLTHAGNALLPLIATTAVAYGVNVLWMKRSIMTEKIA 420 LVCMAGVLGSVLGAPLTAIVFAFGLTHA ALLPLIATTAVAYGVNVL MKRSIMTEKIA Sbjct: 361 LVCMAGVLGSVLGAPLTAIVFAFGLTHASQALLPLIATTAVAYGVNVLLMKRSIMTEKIA 420 Query: 421 RRGLHVHREYGVDPLERVHVDDLMS-VPQALEAALPAGQALEQCRTGAQVHRYYPVTAQG 479 RRGLHVHREYGVDPLER+HV++LM+ PQ L ALPA +AL CR VHRYYPVT Sbjct: 421 RRGLHVHREYGVDPLERMHVEELMTAAPQTLAGALPAAEALAWCRAQEHVHRYYPVTDGV 480 Query: 480 RLVGMVALRRLRQADPATDCGSLLEPDSGYLLPHLSARAAAGAMAQRAVARLPVVAGPEK 539 LVGM+ALR L AD + C +L + YLLPH+SAR+AAG MA+ VARLPVVAGPE+ Sbjct: 481 HLVGMLALRALEHADASQPCDTLAQGHEHYLLPHMSARSAAGMMARLGVARLPVVAGPER 540 Query: 540 QQLVGVLSLSDLARASATHAEEESVREKLLGN 571 ++LVGVLSLSDLARASA HAEEESVRE+LLG Sbjct: 541 RKLVGVLSLSDLARASAAHAEEESVRERLLGG 572 >gi|300311435|ref|YP_003775527.1| chloride channel protein [Herbaspirillum seropedicae SmR1] Length = 555 Score = 104 bits (259), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 144/516 (27%), Positives = 230/516 (44%), Gaps = 36/516 (6%) Query: 62 LGWIAAVL-PVAGGLIVGLMARYGSDKIRGHGIPEAIEAILFGKSRMSPKVALLKPLSSG 120 L W VL P GG++ GL + G+G + +EA+ G + + LL+ +SS Sbjct: 44 LPWYTRVLLPTCGGVVAGLFLVWAKRTPAGNG-GDYMEAVAIGDGAIPVRNTLLRSISSL 102 Query: 121 IVIGSGGPFGAEGPIIMTGGSLGSLLAQWLHLTAAERKTLLVSGACAGMTAIFGTPLAAV 180 I SGG G EGP++ SL+ ++ H ++ + L+ GA AG+T+ + P+A Sbjct: 103 ASIASGGSIGREGPMVQLSALCASLVGKFSHFPSSRLRLLVACGAAAGITSAYNAPIAGA 162 Query: 181 LLAVELLLFELRPRSLLPVALACAVAGFLRPWMIDAGVLFPLQVAP-VAPLALLSCLLAG 239 E++L L S PV +A VA + + + P + ++ L+ G Sbjct: 163 FFITEIVLGSLVMESFGPVVVASVVANITMRELPGYRAAYEMPYFPEIGGWEVMLFLVLG 222 Query: 240 LASGVLAALLTLALYRIEDGFHRLPLHWMWWPALGGIAVGLGGLLQPRALGVGYDVIADL 299 + +GVLA L + GF +L L +GG+ VGL + P G GY ++ L Sbjct: 223 VLAGVLAPQFLRVLELGKHGFGKLRLPLPVRLGVGGLLVGLISVQVPEVWGNGYSLVNSL 282 Query: 300 LNQHMLVSAALVLLMVKAVIWLLALGSGTSGGVLAPLLMMGAGLGLVL----SPWLPG-- 353 L+ + A L++L+VK + + +GSG GG+ P L +GA +G + LP Sbjct: 283 LHTSWVWQAVLMVLVVKVLATSITVGSGAVGGIFTPTLFVGAAVGYLFGDAAQALLPVHI 342 Query: 354 GSPTLWPLVCMAGVLGSVLGAPLTAIVFAFGLTHAGNALLPLIATTAVAYGVNVLWMKRS 413 P + +V M L + APL AI+ F +T + +LPL+ + VAY V RS Sbjct: 343 SQPFAYAMVGMGAFLAAASYAPLMAILMIFEMTLSYQVVLPLMLSCVVAYVV-----ARS 397 Query: 414 IMTEKIARRGLHVHREYGVDPLERV-----HVDDLMSVPQALEAALPAGQALEQCRT--- 465 + + L HR D ER+ H+ +L+ + E L +LE+ Sbjct: 398 VDGRSMYEITLKRHR----DNEERLRLRGTHMSELI---RPAETVLHEEASLEEISALFL 450 Query: 466 GAQVHRYYPVTAQGRLVGMVALRRLRQ--ADPATDCG----SLLEPDSGY-LLPHLSARA 518 V Y V + R +G+VAL+ + D G L P + + P +S Sbjct: 451 KYPVKYVYIVDGRQRYLGVVALQDITSWLLDKKEAAGRVARDFLRPHFLHEVTPDMSLGE 510 Query: 519 AAGAMAQRAVARLPVVAGPEKQQLVGVLSLSDLARA 554 A RLPV+A + QL+GV+ + L A Sbjct: 511 ALQLFLDHQGERLPVIASADDPQLLGVVFKTSLLDA 546 >gi|300311782|ref|YP_003775874.1| chloride channel protein [Herbaspirillum seropedicae SmR1] Length = 442 Score = 61.6 bits (148), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 91/326 (27%), Positives = 146/326 (44%), Gaps = 34/326 (10%) Query: 34 LIKLIALFTNLFYFQRLSFAASSPASHTLGWIAAVLPVAGGLIVGLMARYGSDKIRGHG- 92 LI +A + F+ L +A H WI +LP+ GG VG + + + Sbjct: 25 LIAALAGSASAFFLFALDWATHWRERHA--WIIWLLPL-GGFAVGWLYLHTGKPVEAGNN 81 Query: 93 --IPEAIEAILFGKSRMSPKVALLKPLSSGIVIGSGGPFGAEGPIIMTGGSLGSLLAQWL 150 I E + RM+P V LL ++S + G G EG + GG+L L L Sbjct: 82 LLIDEIHDPRKVIPLRMAPLV-LLGTVASHLF---GASVGREGTAVQMGGALADQLTHLL 137 Query: 151 HLTAAERKTLLVSGACAGMTAIFGTPLAAVLLAVELL-LFELRPRSLLPVALACAVAGFL 209 L +R+ LL++G AG +++FGTP+A + +E+L + +R ++ P +A A + Sbjct: 138 RLRPEDRRILLMAGISAGFSSVFGTPMAGAIFGLEVLAIGRMRYEAIFPCFVAAIAADQV 197 Query: 210 R-PWMIDAGVLFPLQVAPVAPLALLSCLLA-------GLASGVLA-ALLTLALYRIEDGF 260 W + + + + AP+AL S GLA + A + TL+ + ++ Sbjct: 198 GLAWGVHH-THYAMNAS--APMALWSVAAVIAAGVVFGLAGMIFANSTHTLSAF-MKRHV 253 Query: 261 HRLPLHWMWWPALGGIAVGLG--GLLQPRALGVGYDVIADLLNQHMLVSAALVLLMVKAV 318 PL P +GG V L + R +G+G VI + Q + A + K V Sbjct: 254 SYAPLR----PFIGGFIVALAVWAVGTHRYIGLGIPVIVEAFQQPL----APWDFLGKMV 305 Query: 319 IWLLALGSGTSGGVLAPLLMMGAGLG 344 + +LGSG GG + PL +GA LG Sbjct: 306 FTVTSLGSGFKGGEVTPLFYIGATLG 331 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.139 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,638,827 Number of extensions: 66371 Number of successful extensions: 419 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 408 Number of HSP's successfully gapped: 4 Length of query: 571 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 481 Effective length of database: 1,193,720 Effective search space: 574179320 Effective search space used: 574179320 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 133 (55.8 bits)