BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1467.p (375 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310902|ref|YP_003774994.1| alkanesulfonate monooxygenase [... 729 0.0 gi|300310978|ref|YP_003775070.1| alkanesulfonate monooxygenase [... 447 e-127 gi|300310284|ref|YP_003774376.1| coenzyme F420-dependent N5,N10-... 130 1e-31 gi|300314031|ref|YP_003778123.1| coenzyme F420-dependent N5,N10-... 72 1e-13 gi|300313871|ref|YP_003777963.1| thiophene oxidase [Herbaspirill... 62 1e-10 >gi|300310902|ref|YP_003774994.1| alkanesulfonate monooxygenase [Herbaspirillum seropedicae SmR1] Length = 375 Score = 729 bits (1883), Expect = 0.0, Method: Compositional matrix adjust. Identities = 358/375 (95%), Positives = 367/375 (97%) Query: 1 MSLELFWFLPTSGDTRYLGKSDFGRAPTLDYMRQIAVTSENLGYDGLLIPTGASCLDPWI 60 MSLELFWFLPTSGDTRYLGKSDFGRAPTL+YMRQIAVTSE LGYDGLLIPTGASCLDPW+ Sbjct: 1 MSLELFWFLPTSGDTRYLGKSDFGRAPTLEYMRQIAVTSETLGYDGLLIPTGASCLDPWV 60 Query: 61 VAASLVPVTQGIKLLVALRTSLGGPGPSARQAATLDQALNGRLLLNVVPGGDATELEADG 120 VAASLVPVTQ IKLLVALRTSLGGPGPSARQAATLDQALNGRLLLNVVPGGDATELEADG Sbjct: 61 VAASLVPVTQRIKLLVALRTSLGGPGPSARQAATLDQALNGRLLLNVVPGGDATELEADG 120 Query: 121 VFLKHDERYEAADEYLTIWRKLLQGEIVNYEGKHFRIRRGQIFHPAVQQPYPPLYFGGSS 180 VFLKHDERYEAADEYLTIWRKLLQGE VNYEGKHFRIR GQ+FHPAVQQPYPPLYFGGSS Sbjct: 121 VFLKHDERYEAADEYLTIWRKLLQGETVNYEGKHFRIRNGQLFHPAVQQPYPPLYFGGSS 180 Query: 181 PAAHALAAKHVDAYLTWGEPPAAVKQKIEDVRERAAKHGRTVRFGVRLHAIVRETTAEAW 240 PAAH LAAKHVDAYLTWGEPPAAV+QKI+DVRERAA+ GRTVRFGVRLHAIVRETTAEAW Sbjct: 181 PAAHDLAAKHVDAYLTWGEPPAAVRQKIDDVRERAARQGRTVRFGVRLHAIVRETTAEAW 240 Query: 241 AAADRLISKLTDDDIARAQENYARMDSVGQQRMAALHGGRRDKLEIAPNLWAGVGLVRGG 300 AA+RLISKLTD+DIARAQ+NYARMDSVGQQRMAALHGGRRDKLEIAPNLWAGVGLVRGG Sbjct: 241 TAAERLISKLTDEDIARAQQNYARMDSVGQQRMAALHGGRRDKLEIAPNLWAGVGLVRGG 300 Query: 301 AGTSLVGDAATVAERLQEYADLGVDTFVLSGYPHLEEAIRFAELVFPLLGKNAVTLRDRA 360 AGTSLVGDA TVAERLQEYADLGVDTFVLSGYPHLEEAIRFAELVFPLLGKNAVTLRDRA Sbjct: 301 AGTSLVGDATTVAERLQEYADLGVDTFVLSGYPHLEEAIRFAELVFPLLGKNAVTLRDRA 360 Query: 361 QTGGAFDIRAAASAS 375 QTGGAFDIRAAASAS Sbjct: 361 QTGGAFDIRAAASAS 375 >gi|300310978|ref|YP_003775070.1| alkanesulfonate monooxygenase [Herbaspirillum seropedicae SmR1] Length = 388 Score = 447 bits (1151), Expect = e-127, Method: Compositional matrix adjust. Identities = 229/351 (65%), Positives = 275/351 (78%), Gaps = 4/351 (1%) Query: 3 LELFWFLPTSGDTRYLGKSDFGRAPTLDYMRQIAVTSENLGYDGLLIPTGASCLDPWIVA 62 + +FWF+PT GD+RYLG S+ RA + DYM+Q+AV ++ LGYDG+L+PTG SC D W+ A Sbjct: 1 MNVFWFIPTHGDSRYLGTSEGARAVSHDYMKQVAVAADTLGYDGVLLPTGRSCEDAWVAA 60 Query: 63 ASLVPVTQGIKLLVALRTSLGGPGPSARQAATLDQALNGRLLLNVVPGGDATELEADGVF 122 ASL+ T+ +K LVA+R L P +AR AAT D+ GRLL+NVV GGD E E+DGVF Sbjct: 61 ASLIDATRRLKFLVAVRPGLMAPTLAARMAATFDRLSGGRLLINVVTGGDPVEQESDGVF 120 Query: 123 LKHDERYEAADEYLTIWRKLL----QGEIVNYEGKHFRIRRGQIFHPAVQQPYPPLYFGG 178 H ERYE +DE+L IWR +L GE +++GKH ++ + +P VQQPYPPLYFGG Sbjct: 121 GSHAERYEISDEFLKIWRDVLTKTRSGEPTHFQGKHLSVKGAKALYPPVQQPYPPLYFGG 180 Query: 179 SSPAAHALAAKHVDAYLTWGEPPAAVKQKIEDVRERAAKHGRTVRFGVRLHAIVRETTAE 238 SS A LAA+ VD YLTWGEPPAAV +KI VRERAAK GRT+RFG+RLH IVRET Sbjct: 181 SSDEAIDLAAEQVDVYLTWGEPPAAVAEKIARVRERAAKAGRTLRFGIRLHVIVRETNEA 240 Query: 239 AWAAADRLISKLTDDDIARAQENYARMDSVGQQRMAALHGGRRDKLEIAPNLWAGVGLVR 298 AW AAD LIS+L D+ IA+AQ N+ARMDS GQ+RMAALHGGRRDKLE++PNLWAGVGLVR Sbjct: 241 AWRAADELISRLDDETIAKAQANFARMDSEGQRRMAALHGGRRDKLEVSPNLWAGVGLVR 300 Query: 299 GGAGTSLVGDAATVAERLQEYADLGVDTFVLSGYPHLEEAIRFAELVFPLL 349 GGAGT+LVGD TVAERLQEYA LG+DTF+LSGYPHLEE+ RFAELVFPLL Sbjct: 301 GGAGTALVGDGPTVAERLQEYAALGIDTFILSGYPHLEESYRFAELVFPLL 351 >gi|300310284|ref|YP_003774376.1| coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase [Herbaspirillum seropedicae SmR1] Length = 385 Score = 130 bits (328), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 102/331 (30%), Positives = 167/331 (50%), Gaps = 20/331 (6%) Query: 28 TLDYMRQIAVTSENLGYDGLLIPT------GASCLDPWI-VAASLVPVTQGIKLLVALRT 80 +L+Y +++AVT+E G++ L GA + + +L+ T +K+L A+ Sbjct: 42 SLEYNQRLAVTAEKAGFEYALSQIRFTAGYGAEYQHESVSFSQALLHATTKLKVLAAILP 101 Query: 81 SLGGPGPSARQAATLDQALNGRLLLNVVPGGDATELEADGV-FLKHDERYEAADEYLTIW 139 P A+Q AT+D NGR+ +NVV G E +A G +L+HDERY ++E++ Sbjct: 102 GPWHPAVVAKQIATIDHISNGRIAINVVSGWFKGEFQAIGEPWLEHDERYRRSNEFIRAL 161 Query: 140 RKLLQGEIVNYEGKHFRIRRGQIFHPAVQQPYPPLYFGGSSPAAHALAAKHVDAYLTWGE 199 + + + ++G +R + VQ+P+P ++ GGSS AA AA D Y T G Sbjct: 162 KGIWTQDDFTFKGDFYRFNNYTLSPKPVQKPHPEIFQGGSSRAARDNAASVSDWYFTNGN 221 Query: 200 PPAAVKQKIEDVRERAAKHGRTVRFGVRLHAIVRETTAEAWAAADRLISKLTDDDIARAQ 259 +K +I+D++ +AA++G VR GV I R+T EA A +I D A + Sbjct: 222 TLEGLKAQIDDIQAKAAQNGHKVRIGVNAFVIARDTEEEAQAVLQDII------DHAHVE 275 Query: 260 ENYARMDSVGQQRMAALHGGRRDKLEIAPNLWAGVGLVRGGAGTSLVGDAATVAERLQEY 319 +A D+V Q A+ G + A + + + G T+L+G +AER+ Sbjct: 276 AVHAFGDAVKQAGKASPEG----EGNWAKSTFEDLVQYNDGFKTNLIGTPQQIAERIVAL 331 Query: 320 ADLGVDTFVLSGYPH-LEEAIRFAELVFPLL 349 +GVD VL+G+ H +EE F + V PL+ Sbjct: 332 KAIGVD-LVLTGFLHFIEEVEYFGQKVLPLV 361 >gi|300314031|ref|YP_003778123.1| coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase [Herbaspirillum seropedicae SmR1] Length = 459 Score = 71.6 bits (174), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 81/347 (23%), Positives = 144/347 (41%), Gaps = 46/347 (13%) Query: 57 DPWIVAASLVPVTQGIKLLVALRTSLGGPGPSARQAATLDQALNGRLLLNVVPGGDATEL 116 DP ++ ++ VT+ + V + P AR+ +TLD GR+ N+V G + Sbjct: 85 DPLMLVPAMAAVTEHLGFGVTANLTYEAPFLFARRMSTLDHLSRGRVGWNIVTGYLDSAA 144 Query: 117 EADGVF--LKHDERYEAADEYLTIWRKLLQG----------------------EIVNYEG 152 A G+ L+HD+RY+ ADE++ + KL +G + + G Sbjct: 145 RAMGLHEQLEHDQRYDRADEFMEVVYKLWEGSWEDGAVLRDRARRVYADPAKVHKIAHHG 204 Query: 153 KHFRIRRGQIFHPAVQQPYPPLYFGGSSPAAHALAAKHVDAYLTWGEPPAAVKQKIEDVR 212 ++ + + P+ Q+ P LY GSS A A+H + + A ++ ++ +R Sbjct: 205 DYYHVEGIHLSEPSRQRT-PVLYQAGSSGRGQAFGARHAECVFISSQSKDATRKLVQSIR 263 Query: 213 ERAAKHGR---TVRFGVRLHAIVRETTAEAWAAADRLISKLTDDDIARAQ---------E 260 + A + GR ++ + L + T +A R ++ + A Sbjct: 264 DGAVREGRDPDAIKIFMGLTVVTGRTEQQAQERY-REYARYASPEAGLAHYSAGTGIDFS 322 Query: 261 NYARMDSVGQQRMAALHGGRRDKLEIAPNL-WAGVGLVR----GGAGTSLVGDAATVAER 315 Y + + + A+ K+ P L W L++ GG S+VG VA+ Sbjct: 323 QYELDEPIRPAKTNAIES--LTKVVTQPELNWTKRKLLQQLELGGRYASIVGAPDRVADE 380 Query: 316 LQEY-ADLGVDTFVLSGYPHLEEAIRFAELVFPLLGKNAVTLRDRAQ 361 L+ + AD G+D + L+ E F ELV P+L + R+ AQ Sbjct: 381 LESWVADTGIDGYNLTRTVTPESFEDFVELVIPVLQERGSYKREYAQ 427 >gi|300313871|ref|YP_003777963.1| thiophene oxidase [Herbaspirillum seropedicae SmR1] Length = 433 Score = 61.6 bits (148), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 96/354 (27%), Positives = 143/354 (40%), Gaps = 55/354 (15%) Query: 53 ASCLDPWIVAASLVPVTQGIKLLVALRTSLGGPGPSARQAATLDQALNGRLLLNVVPGGD 112 A+ LDP + +L T I L T+ P AR+ ATLD GR NVV Sbjct: 77 ATRLDPLPILGALAAATSHIGLGATRSTTYSQPYSLAREFATLDHLSGGRAAWNVVTS-- 134 Query: 113 ATELEADGVFLK----HDERYEAADEYLTIWRKL----------LQGE---------IVN 149 + EAD LK HD RY+ ADE+L + KL LQ + ++ Sbjct: 135 VNQGEADNFGLKETLPHDVRYDRADEFLEVTHKLWHSWQPDALRLQQDRYADADKVAAID 194 Query: 150 YEGKHFRIRRGQIFHPAVQQPYPPLYFGGSSPAAHALAAKHVDAYLTWGEPPAAVKQKIE 209 ++G HF + RG + PA Q +P + GSS AA+ + ++ Sbjct: 195 HQGAHFSV-RGPLNIPASPQGHPVIIQAGSSDRGQDFAARWAEVVFNIQPDLPRLQAFYR 253 Query: 210 DVRERAAKHGRTVRFGVRLHAIV----------RETTAEAWAAADRLISKLTDDDIARAQ 259 D++ RA + GR + L A++ RE +A A AD L+ T Sbjct: 254 DLKNRAQQFGRRPQDIRILSAVMPFVGQSRQHARELQEQANALADPLVGLSTLSSHMNVD 313 Query: 260 ENYARMDS-VGQQRMAALHG----------GRRDKLEIAPNLWAGVGLVRGGAGTSLVGD 308 + +D+ +G +++ + G R+ L A L+A +G + G Sbjct: 314 FSGFALDAPLGDVQVSGIQGLFKLLRAISAERQLTLADAGRLYA-----QGVLTPQIAGT 368 Query: 309 AATVAERLQE-YADLGVDTFVLSGYPHLEEAI-RFAELVFPLLGKNAVTLRDRA 360 AA VA+ LQ G D FV+S HL F + V P L + +D A Sbjct: 369 AADVADWLQSIIVANGADGFVISP-SHLPYGFDDFVDQVIPELQRRGAFRQDYA 421 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,275,578 Number of extensions: 56848 Number of successful extensions: 184 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 180 Number of HSP's successfully gapped: 5 Length of query: 375 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 288 Effective length of database: 1,207,925 Effective search space: 347882400 Effective search space used: 347882400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)