BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1466.p (125 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310901|ref|YP_003774993.1| glycine cleavage system protein... 236 3e-64 >gi|300310901|ref|YP_003774993.1| glycine cleavage system protein H [Herbaspirillum seropedicae SmR1] Length = 125 Score = 236 bits (603), Expect = 3e-64, Method: Compositional matrix adjust. Identities = 112/125 (89%), Positives = 120/125 (96%) Query: 1 MNIPDHLVYAQTHEWVKLEDDGTLTIGITDFGQEQLGPLVYVDMPSVGKALARGAESGIV 60 M+IPDHLVYAQTHEWVKLEDDGTLTIGITDFGQEQLGPLVYVDMP+VGK LARGAESGIV Sbjct: 1 MHIPDHLVYAQTHEWVKLEDDGTLTIGITDFGQEQLGPLVYVDMPAVGKVLARGAESGIV 60 Query: 61 ESNKTASDLHAPVDGEVIAINEVLVERPDAINEAPYEQWIFKLKPTAPFSSEGFLDAAAY 120 ESNKTASDLHAPVDGEV+A+NE+LVE PDAIN APYEQWIFKLKP APFS+EGFLDAA Y Sbjct: 61 ESNKTASDLHAPVDGEVVAVNELLVETPDAINGAPYEQWIFKLKPAAPFSAEGFLDAAGY 120 Query: 121 IKLVS 125 +KL+S Sbjct: 121 LKLIS 125 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.134 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 425,246 Number of extensions: 16816 Number of successful extensions: 30 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 30 Number of HSP's successfully gapped: 1 Length of query: 125 Length of database: 1,619,870 Length adjustment: 75 Effective length of query: 50 Effective length of database: 1,264,745 Effective search space: 63237250 Effective search space used: 63237250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 125 (52.8 bits)