BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1445.p (176 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313043|ref|YP_003777135.1| two component response regulato... 120 5e-29 >gi|300313043|ref|YP_003777135.1| two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 236 Score = 120 bits (302), Expect = 5e-29, Method: Compositional matrix adjust. Identities = 63/162 (38%), Positives = 90/162 (55%) Query: 2 VADLRQKFPMLGIVVIGENISEATQEKTLHDGADYFLHQPVREGLLLATIRSFLRRLHIL 61 +A LRQ FP +GIVV+ + + + DGADY+L +PV+ + AT+ + RR+ + Sbjct: 63 LASLRQHFPSMGIVVLTAAQRMEERIRCMSDGADYYLARPVKLEEVSATLTALARRMKTV 122 Query: 62 QTPAPGNTEAWTLYRRNGTLVSPHNERLALSDKECAVLTTLFLSPHFPVSAEHLMHALNI 121 + +E W RR+G L S L +DKE L L SP++PVS L L Sbjct: 123 PHQSSPQSEKWAFNRRSGRLSSNGVTALQFTDKESTALAALLQSPNYPVSHGQLFDLLRS 182 Query: 122 TAEIVDPHRIDTIIYRIRKKLAQLAHTRFEIRNIYGRGFICV 163 E D HRID +IYR+R+KL L + +IRNIYG GF+ + Sbjct: 183 GGEEYDAHRIDALIYRVRQKLRSLEESPMQIRNIYGEGFLML 224 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.140 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 559,758 Number of extensions: 21739 Number of successful extensions: 104 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 106 Number of HSP's successfully gapped: 2 Length of query: 176 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 97 Effective length of database: 1,245,805 Effective search space: 120843085 Effective search space used: 120843085 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 127 (53.5 bits)