BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1444.p (215 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protei... 176 1e-45 gi|300310189|ref|YP_003774281.1| transmembrane protein [Herbaspi... 141 4e-35 gi|300313074|ref|YP_003777166.1| GGDEF domain-containing protein... 133 1e-32 gi|300310170|ref|YP_003774262.1| diguanylate cyclase/phosphodies... 132 3e-32 gi|300311036|ref|YP_003775128.1| signal transduction protein [He... 132 3e-32 >gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 695 Score = 176 bits (446), Expect = 1e-45, Method: Compositional matrix adjust. Identities = 97/200 (48%), Positives = 129/200 (64%), Gaps = 11/200 (5%) Query: 6 ALSCALLGAVAVIGVLGGLLRKARRRCDRLDQQLADAKSVNASLRQQALYDSLTGLPNRN 65 AL+ ALL +V L + R +L + LA A N L Q L+D LT LPNR Sbjct: 230 ALTIALLTSV--------LDARLESRTAKLARSLAAA---NEELTQMVLHDQLTRLPNRT 278 Query: 66 LLEDRISEAIARAEREQGRFALLFLDLDGFKAVNDRLGHAAGDLLLMQIGERLSESLRHA 125 LLEDR+++AI +A REQG FAL+F+DLDGFKA+ND LGH GD LL+++ +RL+ S R Sbjct: 279 LLEDRLNQAINKAAREQGHFALMFMDLDGFKAINDSLGHHVGDRLLVEVAQRLTSSSRAN 338 Query: 126 DTIARIGGDEFVVLSEIVACGDITIIRDKLTQALTQPFPIGPTSLNVSASLGHAHYPEDG 185 DT+AR+GGDEFV+L ++ D + +KL +A+ QPF + L VSAS+G A YPEDG Sbjct: 339 DTVARLGGDEFVILVDLNTPEDAMGVAEKLVEAVNQPFKVDQHELRVSASVGIAIYPEDG 398 Query: 186 LSMEALLARADQGMYHLKRT 205 + L+ AD MYH KR+ Sbjct: 399 ATRHDLVINADAAMYHTKRS 418 >gi|300310189|ref|YP_003774281.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 706 Score = 141 bits (356), Expect = 4e-35, Method: Compositional matrix adjust. Identities = 76/161 (47%), Positives = 103/161 (63%), Gaps = 1/161 (0%) Query: 46 NASLRQQALYDSLTGLPNRNLLEDRISEAIARAEREQGRFALLFLDLDGFKAVNDRLGHA 105 N L A +D LTGLPNR LL DRI A+ A R FA+LF+DLDGFKA+ND LGHA Sbjct: 255 NDKLLHFATHDVLTGLPNRALLADRIQHAVEVARRNGKTFAVLFMDLDGFKAINDSLGHA 314 Query: 106 AGDLLLMQIGERLSESLRHADTIARIGGDEFVVLSEIVACGDIT-IIRDKLTQALTQPFP 164 GD LL+ + +R+ + LR D +ARIGGDEFVV+ ++ D+ + + + AL Q F Sbjct: 315 VGDGLLVAVAQRIRQCLRGEDMVARIGGDEFVVVVGNLSSPDVVEQLSENILAALRQDFQ 374 Query: 165 IGPTSLNVSASLGHAHYPEDGLSMEALLARADQGMYHLKRT 205 I +L V++S+G A YP G S++AL+ AD MY K++ Sbjct: 375 IDDATLRVTSSIGIAVYPNSGDSVDALMKNADAAMYEAKQS 415 >gi|300313074|ref|YP_003777166.1| GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 738 Score = 133 bits (334), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 70/160 (43%), Positives = 101/160 (63%), Gaps = 4/160 (2%) Query: 49 LRQQALYDSLTGLPNRNLLEDRISEAIARAEREQGRFALLFLDLDGFKAVNDRLGHAAGD 108 +R+ AL D LT LPNR DR+ E + RA R AL F+DLDGFK +NDR GH AGD Sbjct: 565 MRELALTDVLTSLPNRRAFSDRLPEGMMRARRAHQLLALFFMDLDGFKGINDRYGHEAGD 624 Query: 109 LLLMQIGERLSESLRHADTIARIGGDEFVVLSE-IVACGDITIIRDKLTQALTQPFPIGP 167 LL++ RL S+R DT+AR+ GDEFVV+ E + + D ++ +K+ A+ +PF + Sbjct: 625 ELLVEFARRLQASVRDVDTVARLAGDEFVVILENLQSQQDAIMVAEKILHAIREPFELTH 684 Query: 168 TSLNVSASLGHAHYPED---GLSMEALLARADQGMYHLKR 204 +++++S+S+G A Y D + E LL+ AD+ MY KR Sbjct: 685 STVHISSSIGVAVYQPDLDGEIGPEELLSMADKAMYAAKR 724 >gi|300310170|ref|YP_003774262.1| diguanylate cyclase/phosphodiesterase A protein [Herbaspirillum seropedicae SmR1] Length = 1002 Score = 132 bits (331), Expect = 3e-32, Method: Compositional matrix adjust. Identities = 73/157 (46%), Positives = 95/157 (60%) Query: 47 ASLRQQALYDSLTGLPNRNLLEDRISEAIARAEREQGRFALLFLDLDGFKAVNDRLGHAA 106 A + Q A YDSLTGLPNR+LL + +AIA AER A++F+DLD FK VND LGH A Sbjct: 552 ARIHQLAFYDSLTGLPNRSLLLVQAGQAIAAAERNCSTVAVMFIDLDRFKQVNDSLGHQA 611 Query: 107 GDLLLMQIGERLSESLRHADTIARIGGDEFVVLSEIVACGDITIIRDKLTQALTQPFPIG 166 GD LL + RL R +D + R+ GDEFVV+ +T + ++ + L P I Sbjct: 612 GDELLRTVATRLQSQARRSDIVGRLSGDEFVVVLTQYDTQHLTEVVKRIQERLAMPCHIN 671 Query: 167 PTSLNVSASLGHAHYPEDGLSMEALLARADQGMYHLK 203 T LN SAS+G A +P++G +E LL RAD MY K Sbjct: 672 GTPLNPSASIGVAIFPDNGNDIETLLHRADIAMYQAK 708 >gi|300311036|ref|YP_003775128.1| signal transduction protein [Herbaspirillum seropedicae SmR1] Length = 750 Score = 132 bits (331), Expect = 3e-32, Method: Compositional matrix adjust. Identities = 72/162 (44%), Positives = 99/162 (61%), Gaps = 1/162 (0%) Query: 43 KSVNASLRQQALYDSLTGLPNRNLLEDRISEAIARAEREQGRFALLFLDLDGFKAVNDRL 102 K V LR QA YD L+GLPNR LL D + +A A++ A++F+DLD FK VND L Sbjct: 303 KEVEFKLRHQANYDPLSGLPNRRLLGDMLQREVASAKKHAYPLAVVFIDLDRFKDVNDSL 362 Query: 103 GHAAGDLLLMQIGERLSESLRHADTIARIGGDEFV-VLSEIVACGDITIIRDKLTQALTQ 161 GH+ GD LL+ +R+ S+R D +AR+ GDEFV VL + A +T I D + AL + Sbjct: 363 GHSVGDQLLVVAAQRVRASVREHDLVARLAGDEFVAVLPRLEASAALTRIGDDIVAALAE 422 Query: 162 PFPIGPTSLNVSASLGHAHYPEDGLSMEALLARADQGMYHLK 203 PF +G + +SAS+G A YP D ++ L+ ADQ M+ K Sbjct: 423 PFQLGEHRVRISASVGIAIYPHDADTVTTLIGCADQAMFAAK 464 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 554,988 Number of extensions: 21175 Number of successful extensions: 162 Number of sequences better than 1.0e-08: 39 Number of HSP's gapped: 126 Number of HSP's successfully gapped: 39 Length of query: 215 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 134 Effective length of database: 1,236,335 Effective search space: 165668890 Effective search space used: 165668890 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 128 (53.9 bits)