BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1443.p (544 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310882|ref|YP_003774974.1| 2-polyprenyl-6-methoxyphenol hy... 943 0.0 gi|300309878|ref|YP_003773970.1| 2-polyprenyl-6-methoxyphenol hy... 594 e-171 gi|300313232|ref|YP_003777324.1| 3-(3-hydroxyphenyl) propionate ... 86 7e-18 >gi|300310882|ref|YP_003774974.1| 2-polyprenyl-6-methoxyphenol hydroxylase [Herbaspirillum seropedicae SmR1] Length = 547 Score = 943 bits (2437), Expect = 0.0, Method: Compositional matrix adjust. Identities = 448/541 (82%), Positives = 484/541 (89%) Query: 1 MQSTYTYPRFAYRQSDEQRSGQPQRHPVVIIGAGPIGLTAALECAARGLPVVVLDDNDTV 60 M STYTYPRFAYRQSDEQR +P RHPVV+IGAGPIGLTAALECAARGLPVVVLDDNDTV Sbjct: 1 MHSTYTYPRFAYRQSDEQREARPSRHPVVVIGAGPIGLTAALECAARGLPVVVLDDNDTV 60 Query: 61 SIGSRAVCYAKRPLEIWDRLGVAARMVEQGVSWQLGKVFFEDRQVYQFDLLPESAHKMPA 120 SIGSRAVCYAKRPLEIWDRLGVA RMVE+GVSW+LGKVFFE+R+VY+FDLLPE AHKMPA Sbjct: 61 SIGSRAVCYAKRPLEIWDRLGVAPRMVEKGVSWKLGKVFFEEREVYRFDLLPEHAHKMPA 120 Query: 121 MINLQQYYLEQYMVDACHAHPLIDLRWKHQLLNLKQDDEQVTLTVETPDGVFHMQAQWII 180 MINLQQYYLEQY+V+AC A PLIDLRWKH++++L QDD TL VETPDG+F M+A W+I Sbjct: 121 MINLQQYYLEQYLVEACQAQPLIDLRWKHKMISLHQDDTHATLDVETPDGIFRMEADWVI 180 Query: 181 ACDGANSDTRKMVGAEFTGQFFQDRFLIADVIMKADFPTERWFWFDPPFHRGQSVLLHKQ 240 ACDGANSDTRKMVGAEF+GQFFQDRFLIADVIMKADFP ERWFWFDPPFHRGQSVLLHKQ Sbjct: 181 ACDGANSDTRKMVGAEFSGQFFQDRFLIADVIMKADFPAERWFWFDPPFHRGQSVLLHKQ 240 Query: 241 CDNVWRIDFQLGWDADPEQEKKPENVIPRIQAMLGKDVEFELEWVSVYQFACRRIDNMRY 300 CDNVWRIDFQLGW+ADPE+EKKPE VIPRIQAMLGK+VEFELEWVSVYQFACRRID MRY Sbjct: 241 CDNVWRIDFQLGWEADPEEEKKPEKVIPRIQAMLGKEVEFELEWVSVYQFACRRIDKMRY 300 Query: 301 QRVLFAGDAAHQVSPFGARGANTGVQDIDNLVWKLKLVIDGLAPVSFIDSYHEERAFAAD 360 QRVLFAGDAAHQVSPFGARGANTGVQDIDNLVWKLKLVIDG AP SFIDSYHEERA+AAD Sbjct: 301 QRVLFAGDAAHQVSPFGARGANTGVQDIDNLVWKLKLVIDGKAPPSFIDSYHEERAWAAD 360 Query: 361 DNLRQSTRSTDFITPKSRASKVMRDAVLTLAETEPFARTLVNSGRLSTPTPYVDSSLNTP 420 DNLRQSTRSTDFITPKSRASK +RDAVL LAETE FARTLVNSGRLSTPT YV S LNT Sbjct: 361 DNLRQSTRSTDFITPKSRASKALRDAVLGLAETEEFARTLVNSGRLSTPTSYVHSPLNTA 420 Query: 421 DSALFGGRMAPGTPCADAPILRDGRPSWLLEQFGESFTVLVFGAVEVPASIEAAGLTVKV 480 D+ALFGGRMAPGT CADAP+LR+G+P WLLE G F VL FGA A IEAAG++ +V Sbjct: 421 DAALFGGRMAPGTVCADAPVLREGQPGWLLESLGAGFVVLFFGASAPQAVIEAAGISARV 480 Query: 481 KRIGASGSGAELVDVQGLAAQRYGARDGCAYLIRPDQHVAARWLHFDAAAIEQAIARCVG 540 +GA GSGAEL+D +GLAA RY A +GC YL+RPDQHVAARW F + I A+ARCVG Sbjct: 481 VTVGAPGSGAELIDREGLAALRYAASEGCVYLVRPDQHVAARWNRFHSDDIAAALARCVG 540 Query: 541 H 541 Sbjct: 541 Q 541 >gi|300309878|ref|YP_003773970.1| 2-polyprenyl-6-methoxyphenol hydroxylase [Herbaspirillum seropedicae SmR1] Length = 589 Score = 594 bits (1531), Expect = e-171, Method: Compositional matrix adjust. Identities = 297/547 (54%), Positives = 378/547 (69%), Gaps = 28/547 (5%) Query: 5 YTYPRFAYRQSDEQRS-GQPQRHPVVIIGAGPIGLTAALECAARGLPVVVLDDNDTVSIG 63 Y RFA+ EQ+S G HPVVIIGAGP+GL A++ A +G+ VV++DD+DT+S G Sbjct: 9 YQQQRFAWTPCAEQQSPGHAPVHPVVIIGAGPVGLACAIDLARQGIAVVLVDDDDTLSTG 68 Query: 64 SRAVCYAKRPLEIWDRLGVAARMVEQGVSWQLGKVFFEDRQVYQFDLLPESAHKMPAMIN 123 SRA+C+AKR L+I+DR+G RMV++GV W GKVF D+ VY+FDLLPES H PA IN Sbjct: 69 SRAICFAKRTLDIFDRIGCGQRMVDKGVGWNRGKVFLRDQAVYEFDLLPESGHARPAFIN 128 Query: 124 LQQYYLEQYMVDACHAHPLIDLRWKHQLLNLKQDDEQVTLTVETPDGVFHMQAQWIIACD 183 LQQYY+E Y+++ P I++RWK+++ + ++ LTV+TP G + ++A++++A D Sbjct: 129 LQQYYVEGYLLEQAQQWPHIEIRWKNKVSAIAHHEDHEALTVDTPQGSYTLKARYVVAAD 188 Query: 184 GANSDTRKMVGAEFTGQFFQDRFLIADVIMKADFPTERWFWFDPPFHRGQSVLLHKQCDN 243 G+ S RKM+G E G F+DRFLIADV M A FP ERWFWFDPPFH QSVLLH+Q D+ Sbjct: 189 GSRSPLRKMMGLESQGHTFRDRFLIADVKMDAPFPAERWFWFDPPFHPQQSVLLHRQPDD 248 Query: 244 VWRIDFQLGWDADPEQEKKPENVIPRIQAMLGKDVEFELEWVSVYQFACRRIDNMRYQRV 303 VWRIDFQLGWDADP EK PE VIPR++A+LG++V F LEWVSVY F+C R++ R+ RV Sbjct: 249 VWRIDFQLGWDADPVLEKTPERVIPRVRALLGEEVSFSLEWVSVYTFSCVRMERFRHGRV 308 Query: 304 LFAGDAAHQVSPFGARGANTGVQDIDNLVWKLKLVIDGLAPVSFIDSYHEERAFAADDNL 363 LF GDAAH VSPFGARGAN+G+QD +NL WKL V+ G AP S +DSY ER +AAD+NL Sbjct: 309 LFVGDAAHCVSPFGARGANSGIQDAENLAWKLAHVLQGKAPDSLLDSYASEREYAADENL 368 Query: 364 RQSTRSTDFITPKSRASKVMRDAVLTLAETEPFARTLVNSGRLSTPTPYVDSSLNTPDSA 423 STRSTDFITPKS S+ RDAVL LA FAR LVNSGRLS P S LNTPD A Sbjct: 369 LNSTRSTDFITPKSAVSRAFRDAVLGLAREHAFARRLVNSGRLSLPATLDQSPLNTPDQA 428 Query: 424 ------LFGGRMAPGTPCADAPILRDGRPSWLLEQFGESFTVLVFGA-VEVPAS------ 470 F G M PG PC DAP+L+ G+P+WLL Q G+ F +++F A ++PA+ Sbjct: 429 AAPEAVAFTGPMRPGAPCCDAPLLQAGQPAWLLPQLGDRFVLMIFAAPAQLPAATRQALA 488 Query: 471 ------------IEAAGLTVKVKRI--GASGSGAELVDVQGLAAQRYGARDGCAYLIRPD 516 I A T + +R+ +G ++ D+ LAAQRY A G YLIRPD Sbjct: 489 ALAQPDALRLLFISAQAATQEDERVLQTLAGQAIQVQDIDTLAAQRYDAHPGSCYLIRPD 548 Query: 517 QHVAARW 523 QHV+ARW Sbjct: 549 QHVSARW 555 >gi|300313232|ref|YP_003777324.1| 3-(3-hydroxyphenyl) propionate hydroxylase [Herbaspirillum seropedicae SmR1] Length = 544 Score = 85.9 bits (211), Expect = 7e-18, Method: Compositional matrix adjust. Identities = 90/344 (26%), Positives = 150/344 (43%), Gaps = 34/344 (9%) Query: 28 VVIIGAGPIGLTAALECAARGLPVVVLDDNDTVSIGSRAVCYAKRPLEIWDRLGVAARMV 87 V+I+GAGP+G+T G+ ++++ N V RAV L + G+A +V Sbjct: 19 VLIVGAGPVGVTLGNLLGTYGISTLLIERNLDVLDYPRAVGIDDEALRTFQSAGMADAIV 78 Query: 88 EQGVSWQLGKVFFEDRQVYQFDLLPESAHKMPAMINL-QQYYLEQYMVDACHAHPLIDLR 146 + +++ DR+ + ++ P + NL Q E + + LR Sbjct: 79 RDVIQNVPMRMYTADRRCFA-EIQPTTREFGWYRRNLFSQPLGEATLRLGLRRFEHVSLR 137 Query: 147 WKHQLLNLKQDDEQVTLTVETPDGV-FHMQAQWIIACDGANSDTR-KMVGAEFTGQFFQD 204 +LL+L QD+ V ++ G ++AQ+++ CDG S R K++ + G+ Sbjct: 138 SGVELLDLVQDEAGVQASIIDEHGAQATLRAQYVVGCDGGRSTVREKILKLPYEGKTHPA 197 Query: 205 RFLIADVIMKADFPTERWFWFDPPFHRGQSVLLHKQCD------------NVWRIDFQLG 252 ++ V+++ D D P+ LH C+ V R +F L Sbjct: 198 KW----VVIECDGDP-----LDAPY-----TALH--CEPQRPYVCLALPYGVRRWEFMLF 241 Query: 253 WDADPEQEKKPENVIPRIQAMLGKDVE-FELEWVSVYQFACRRIDNMRYQRVLFAGDAAH 311 D EQ PE V ++ + +DV+ + VY R ++ R+ AGDAAH Sbjct: 242 PGEDGEQMLAPEKVRELLREHV-RDVDALNIIRARVYTHNSRVAGSLVCGRICLAGDAAH 300 Query: 312 QVSPFGARGANTGVQDIDNLVWKLKLVIDGLAPVSFIDSYHEER 355 P+ +G N G++D NL WKL V+ G + SYHEER Sbjct: 301 ITPPWIGQGLNAGLRDAFNLAWKLAWVLQGRMKPELLASYHEER 344 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,870,762 Number of extensions: 83145 Number of successful extensions: 271 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 269 Number of HSP's successfully gapped: 3 Length of query: 544 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 454 Effective length of database: 1,193,720 Effective search space: 541948880 Effective search space used: 541948880 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)