BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1382.p (660 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310875|ref|YP_003774967.1| acetyl-coenzyme A synthetase [H... 1336 0.0 gi|300312417|ref|YP_003776509.1| propionyl-CoA ligase [Herbaspir... 431 e-121 gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [H... 202 1e-52 gi|300310257|ref|YP_003774349.1| acetyl-coenzyme A synthetase [H... 168 1e-42 gi|300313157|ref|YP_003777249.1| long-chain-fatty-acid-CoA ligas... 131 2e-31 >gi|300310875|ref|YP_003774967.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 660 Score = 1336 bits (3458), Expect = 0.0, Method: Compositional matrix adjust. Identities = 640/660 (96%), Positives = 652/660 (98%) Query: 1 VADIETFKQENRVFAPPAELAKHAAISGMDAYHALNAEFANDYEGTWARLAKDNLKWHKP 60 +ADIETFKQENRVFAPPAEL KHAAISGMDAYHALNAEFA DYEGTWARLAKDNLKWHKP Sbjct: 1 MADIETFKQENRVFAPPAELVKHAAISGMDAYHALNAEFAKDYEGTWARLAKDNLKWHKP 60 Query: 61 FTQTLDESNAPFYKWFADGQVNVSYNCLDVNLENGNADKTAIIFESDDGQVTRVSYRELH 120 FTQTLDESNAPFYKWFADGQVNVSYNCLDVNLENGNADKTA+IFESDDG+VTRVSYRELH Sbjct: 61 FTQTLDESNAPFYKWFADGQVNVSYNCLDVNLENGNADKTAVIFESDDGKVTRVSYRELH 120 Query: 121 QKVCQFANGLKSLGIKKGDRVVIYMPMSVEGVAAMQACARIGATHSVVFGGFSAKSLHER 180 QKVCQFANGLKSLGIKKGDRVVIYMPMSVEGVAAMQACARIGATHSVVFGGFSAKSLHER Sbjct: 121 QKVCQFANGLKSLGIKKGDRVVIYMPMSVEGVAAMQACARIGATHSVVFGGFSAKSLHER 180 Query: 181 VIDAGAVAVVTADYQVRGGKQLPLKSIVDEALAMGGCDTLKQVIVYKRTGAELNWVEGRD 240 VIDAGAVAV+TADYQVRGGKQLPLKSIVDEAL MGGCDTLK V+VYKR GAE+NWVEGRD Sbjct: 181 VIDAGAVAVLTADYQVRGGKQLPLKSIVDEALDMGGCDTLKHVVVYKRAGAEINWVEGRD 240 Query: 241 LWLSDVMANQAATCEPEWVDAEHPLFILYTSGSTGKPKGVQHASAGYLLWAVLTMKWTFD 300 LWLSDV+ANQ+ TCEPEWVDAEHPLFILYTSGSTGKPKGVQHASAGYLLWAVLTMKWTFD Sbjct: 241 LWLSDVVANQSDTCEPEWVDAEHPLFILYTSGSTGKPKGVQHASAGYLLWAVLTMKWTFD 300 Query: 301 IKPDDVYWCTADIGWITGHTYIAYGPLAVGATQIVFEGVPTYPNAGRFWDMVARHKATIF 360 IKPDDVYWCTADIGWITGHTYIAYGPLAVGATQIVFEGVPTYPNAGRFWDMVARHKATIF Sbjct: 301 IKPDDVYWCTADIGWITGHTYIAYGPLAVGATQIVFEGVPTYPNAGRFWDMVARHKATIF 360 Query: 361 YTAPTAIRSLIKAADADEKVHPKQYDLSTLRILGSVGEPINPEAWMWYYKNVGRENCPIV 420 YTAPTAIRSLIKAADADEKVHPKQYDLS+LRILGSVGEPINPEAWMWYYKNVG ENCPIV Sbjct: 361 YTAPTAIRSLIKAADADEKVHPKQYDLSSLRILGSVGEPINPEAWMWYYKNVGHENCPIV 420 Query: 421 DTFWQTETGGHMISPLPGATPQVPGSCTLPLPGITAAIVDETGHDLPNGNGGILVVKRPW 480 DTFWQTETGGHMISPLPGATPQVPGSCTLPLPGITAAIVDETG+D+PNGNGGILVVKRPW Sbjct: 421 DTFWQTETGGHMISPLPGATPQVPGSCTLPLPGITAAIVDETGNDIPNGNGGILVVKRPW 480 Query: 481 PSMIRTIWNDPERFKKSYFPEELGGNIYLAGDGAVRNKETGYFTITGRIDDVLNVSGHRM 540 PSMIRTIWNDPERFKKSYFPEELGG IYLAGDGAVRNKETGYFTITGRIDDVLNVSGHRM Sbjct: 481 PSMIRTIWNDPERFKKSYFPEELGGKIYLAGDGAVRNKETGYFTITGRIDDVLNVSGHRM 540 Query: 541 GTMEIESALVANSIVAEAAVVGKPDETTGESICAFVVLKRPRPTGEEAKQIAKELRDWVA 600 GTMEIESALVANSIVAEAAVVGKPDETTGESICAFVVLKRPRPTG+EAKQIAKELRDWVA Sbjct: 541 GTMEIESALVANSIVAEAAVVGKPDETTGESICAFVVLKRPRPTGDEAKQIAKELRDWVA 600 Query: 601 KEIGPIAKPKEIRFGDNLPKTRSGKIMRRLLRVLAKGEAITQDVSTLENPAILDQLKEAQ 660 KEIGPIAKPKEIRFGDNLPKTRSGKIMRRLLRVLAKGEAITQDVSTLENPAILDQLKEAQ Sbjct: 601 KEIGPIAKPKEIRFGDNLPKTRSGKIMRRLLRVLAKGEAITQDVSTLENPAILDQLKEAQ 660 >gi|300312417|ref|YP_003776509.1| propionyl-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 628 Score = 431 bits (1107), Expect = e-121, Method: Compositional matrix adjust. Identities = 247/634 (38%), Positives = 372/634 (58%), Gaps = 21/634 (3%) Query: 32 YHALNAEFANDYEGTWARLAKDNLKWHKPFTQTLDESNAPFYKWFADGQVNVSYNCLDVN 91 Y + E WA+ A+ + W +PF++TLD S PF +WF DGQ N+ +N +D + Sbjct: 3 YEQFYRQSVEQPEAFWAQEAQ-RIHWQQPFSRTLDYSRPPFARWFIDGQTNLCHNAVDRH 61 Query: 92 LENGNADKTAIIFESDDGQVTRV-SYRELHQKVCQFANGLKSLGIKKGDRVVIYMPMSVE 150 L A + A+I S + RV +Y EL Q+V A +++LG+++GDRV+IYMPM E Sbjct: 62 LPE-RAQQAALIAISTETNSERVYTYAELQQEVMAMAASMQALGVQRGDRVLIYMPMIAE 120 Query: 151 GVAAMQACARIGATHSVVFGGFSAKSLHERVIDAGAVAVVTADYQVRGGKQLPLKSIVDE 210 V AM ACARIGA HSVVFGGF++ SL R+ DA +V+AD RGGK +P K ++DE Sbjct: 121 AVFAMLACARIGAVHSVVFGGFASNSLASRIDDAQPRLIVSADAGSRGGKVIPYKGLLDE 180 Query: 211 ALAMGGCDTLKQVIVYKRTGAELNWVEGRDLWLSDVMANQAATCEP-EWVDAEHPLFILY 269 A+AM + V++ R A + RD+ + + P W+++ +ILY Sbjct: 181 AIAMAQ-HRPQHVLLVDRGLAPMARTAQRDVDYAPLRTQHLGQQVPVTWLESNASSYILY 239 Query: 270 TSGSTGKPKGVQHASAGYLLWAVLTMKWTFDIKPDDVYWCTADIGWITGHTYIAYGPLAV 329 TSG+TGKPKGVQ GY + +M+ F +P + Y+CT+DIGW+ GH+YI YGPL Sbjct: 240 TSGTTGKPKGVQRDVGGYAVALASSMQHIFCGQPGETYFCTSDIGWVVGHSYIVYGPLIA 299 Query: 330 GATQIVFEGVPTYPNAGRFWDMVARHKATIFYTAPTAIRSLIKAADADEKVHPKQYDLST 389 G +++EG+P P+AG +W +V ++K T ++APTAIR L K ++YD S+ Sbjct: 300 GMATVLYEGLPIRPDAGIWWSIVEKYKVTRMFSAPTAIRVLKKQPPECM----ERYDTSS 355 Query: 390 LRILGSVGEPINPEAWMWYYKNVGRENCPIVDTFWQTETGGHMISPLPGA--TPQVPGSC 447 L+ L GEP++ W G P++D +WQTE+G +IS G P GS Sbjct: 356 LKALYLAGEPLDETTSSWI---SGALKVPVIDNYWQTESGWPIISIAKGIDDKPTRLGSP 412 Query: 448 TLPLPGITAAIVDE-TGHDLPNGNGGILVVKRPW-PSMIRTIWNDPERFKKSYFPEELGG 505 +P+PG AI+DE TG G++ ++ P P ++T++ D ERF +Y+ L Sbjct: 413 GVPMPGYRLAILDEATGEPCGPDQKGVVAIEGPLPPGCMQTVYGDDERFVNTYW-RSLPR 471 Query: 506 NIYLAGDGAVRNKETGYFTITGRIDDVLNVSGHRMGTMEIESALVANSIVAEAAVVGKPD 565 +Y D +R+K+ GY+ I GR DDV+NV+GHR+GT EIE ++ ++ V+E AVVG D Sbjct: 472 EVYSTFDWGIRDKD-GYYFILGRTDDVINVAGHRLGTREIEESISSHPNVSEVAVVGVED 530 Query: 566 ETTGESICAFVVLKRPR--PTGEEAKQIAKELRDWVAKEIGPIAKPKEIRFGDNLPKTRS 623 + G+ AF + K+ T E+ K + E+ V K+IG + +P + F LPKTRS Sbjct: 531 KLKGQVAFAFAIPKQAYTIATPEQRKALEAEIMAVVDKQIGAVGRPARVFFVSGLPKTRS 590 Query: 624 GKIMRRLLRVLAKGEAITQDVSTLENPAILDQLK 657 GK++RR ++ + +G D+ T+E+PA L+Q++ Sbjct: 591 GKLLRRAIQSICEGRD-PGDLPTIEDPAALEQVR 623 >gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 549 Score = 202 bits (513), Expect = 1e-52, Method: Compositional matrix adjust. Identities = 174/563 (30%), Positives = 262/563 (46%), Gaps = 44/563 (7%) Query: 73 YKWFADGQVNVSYNCLDVNLENGNADKTAIIFESDDGQVTRVSYRELHQKVCQFANGLKS 132 Y+W N++ D +G+ +TAII E DG VT SY +L +FAN L++ Sbjct: 24 YEWQIPTHFNIAQAACD-RWADGSG-RTAIICEDADGSVTSYSYDQLKILSDRFANALRA 81 Query: 133 LGIKKGDRVVIYMPMSVEGVAAMQACARIGATHSVVFGGFSAKSLHERVIDAGAVAVVTA 192 G+ +GDR+ IY+ +E V A ++GA +F F ++ R+ ++GAVA+VT Sbjct: 82 AGVGRGDRIGIYLSQRIETVITHLAAYKLGAITVPLFYLFGPDAIAYRLDNSGAVALVTD 141 Query: 193 DYQVRGGKQLPLKSIVDEALAMGGCDTLKQVIVYKRTGAELNWVEGRDLWLSDVMANQAA 252 S +++A G L+ +V+ ++L + D W D + A Sbjct: 142 ------------ASGMEKATLAGELPALR--LVFCVESSDLVLPQTTDFW--DRLHAAPA 185 Query: 253 TCEPEWVDAEHPLFILYTSGSTGKPKGVQHASAGYLLWAVLTMKWTFDIKPD--DVYWCT 310 +P A+ P I+YTSG+TGK KG HA LL + ++ + D P D +W Sbjct: 186 ELDPVLTLADDPAMIIYTSGTTGKAKGALHAHR-VLLGHLPGVEVSHDSFPQEGDRFWTP 244 Query: 311 ADIGWITGHTYIAYGPLAVGATQIVFEGVPTYPNAGRFWDMVARHKATIFYTAPTAIRSL 370 AD WI G + L G +V + + +A + ++ARH+ + PTA++ L Sbjct: 245 ADWAWIGGLLDVLLPSLYHGVA-VVARRLEKF-DAAEVFGLLARHQIRNVFFPPTALKML 302 Query: 371 IKAADADEKVHPKQYDLSTLRILGSVGEPINPEAWMWYYKNVGRENCPI-VDTFWQTETG 429 AA Q D S LR + S GE + + W GRE + ++ F+ Sbjct: 303 RGAATVR-----AQADFS-LRSVASGGETLGDDLIAW-----GREALGVTINEFYGQTEC 351 Query: 430 GHMISPLPGATPQVPGSCTLPLPGITAAIVDETGHDLPNGNGGILVVKRPWPSMIRTIWN 489 ++S P V GS +PG IVDE G LP+G G + ++ P P M W Sbjct: 352 NLVVSSSSHCYPSVSGSMGRAVPGHVVQIVDEQGQVLPHGTVGNIAIRAPDPVMFLRYWR 411 Query: 490 DPERFKKSYFPEELGGNIYLAGDGAVRNKETGYFTITGRIDDVLNVSGHRMGTMEIESAL 549 + E + E+ G+ L GD + E GY GR DDV+ +G+R+G IE L Sbjct: 412 NEEATR-----EKFAGDFLLTGDLGSMD-EQGYIRYLGRNDDVITSAGYRIGPAAIEECL 465 Query: 550 VANSIVAEAAVVGKPDETTGESICAFVVLKRPRPTGEEAKQIAKELRDWVAKEIGPIAKP 609 + + V AAVVG D E + AFVVLK E K EL+ V ++ P Sbjct: 466 MRHPAVRIAAVVGVKDALRTEVVKAFVVLKDGVTPDEALK---AELQQHVRAQLAAHEYP 522 Query: 610 KEIRFGDNLPKTRSGKIMRRLLR 632 + I F LP T +GKIMR+ L+ Sbjct: 523 RLISFVAALPTTATGKIMRKTLK 545 >gi|300310257|ref|YP_003774349.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 556 Score = 168 bits (425), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 150/555 (27%), Positives = 242/555 (43%), Gaps = 49/555 (8%) Query: 81 VNVSYNCLDVNLENGNADKTAIIFESDDGQVTRVSYRELHQKVCQFANGLKSLGIKKGDR 140 +N C D +++ D A+ F S G+ + S+ ++ + AN L+ GI+ G Sbjct: 37 INACVECCDRHVKG---DNIALEFVSLHGEHQQYSFAQVRSMAARVANLLRQQGIQPGQI 93 Query: 141 VVIYMPMSVEGVAAMQACARIGATHSVVFGGFSAKSLHERVIDAGAVAVVTADYQVRGGK 200 V +P + E +A + R GA + +F F K++ R+ +GA +VT Sbjct: 94 VAGMLPRTPELLATVLGTLRAGAVYQPLFTAFGPKAIEHRLALSGASLIVT--------- 144 Query: 201 QLPLKSIVDEALAMGGCDTLKQVIVYKRTGAELNWVEGRDLWLSDVMANQAATCEPEWVD 260 + + +DE + C QV + + L D+ + Q+ P Sbjct: 145 NVANRDKLDE---IANC---PQVCTVRESNDPL---RAGDIDFRAAVDAQSDDFAPVLRS 195 Query: 261 AEHPLFILYTSGSTGKPKGVQHASAGYLLWAVLTMKWTFDIKPDDVYWCTADIGWITGHT 320 + ++ TSG+TG KGV + ++V M+ + P D +W AD GW G Sbjct: 196 GADLMLMMSTSGTTGAAKGVPVPLSALQAFSVY-MREAVGLLPQDKFWNMADPGWAYGLY 254 Query: 321 YIAYGPLAVGATQIVFEGVPTYPNAGRFWDMVARHKATIFYTAPTAIRSLIKAADADEKV 380 Y GPLA+G E T ++ + R T APTA R ++ A + Sbjct: 255 YAIIGPLAIGHGITFNEAAFTVSGT---YETIRRLGITSLAGAPTAYRMMMAAGEEAAAS 311 Query: 381 HPKQYDLSTLRILGSVGEPINPEAWMWYYKNVGRENCPIVDTFWQTETGGHMISPLPGAT 440 + LR + S GEP+N E W+ + + PI D + QTE G + + Sbjct: 312 VKGR-----LRAVSSAGEPLNAEVVRWFASAL---DVPIHDHYGQTELGMVVNNHHALRH 363 Query: 441 PQVPGSCTLPLPGITAAIVDETGHDLPNGNGGILVVKRPWPSMIRTIWNDPERFKKSYFP 500 VPGS +PG ++DE +L G L + I P + Y+ Sbjct: 364 EVVPGSAGFAMPGYRVVVLDENHQELGANQPGELAID---------IARSPLYWFSGYWK 414 Query: 501 EE---LGGNIYLAGDGAVRNKETGYFTITGRIDDVLNVSGHRMGTMEIESALVANSIVAE 557 ++ + Y GD V + G + GR DDV+ +G+R+G ++ESAL+ + VA+ Sbjct: 415 QDTPAIAHGYYSTGDN-VELEPNGSISFIGRSDDVITSAGYRIGPFDVESALLEHPAVAD 473 Query: 558 AAVVGKPDETTGESICAFVVLKRPRPTGEEAKQIAKELRDWVAKEIGPIAKPKEIRFGDN 617 AV+G PD E + AFVVL + + + ++L V + + A P+ I F D Sbjct: 474 VAVIGLPDPERTEIVKAFVVLSD---QFKASDALREQLAQHVKRRLSAHAYPRAIEFLDA 530 Query: 618 LPKTRSGKIMRRLLR 632 LPKT SGK+ R +LR Sbjct: 531 LPKTPSGKLQRFVLR 545 >gi|300313157|ref|YP_003777249.1| long-chain-fatty-acid-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 566 Score = 131 bits (330), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 148/537 (27%), Positives = 230/537 (42%), Gaps = 49/537 (9%) Query: 114 VSYRELHQKVCQFANGLKSLGIKKGDRVVIYMPMSVEGVAAMQACARIGATHSVVFGGFS 173 +SYREL L+ LG++KG RV I MP ++ A+ A R G T V ++ Sbjct: 49 LSYRELDSLSRALGGWLQGLGLQKGARVAIMMPNVLQYPIAIAAILRAGYTVVNVNPLYT 108 Query: 174 AKSLHERVIDAGAVAV-VTADYQVRGGKQLP---LKSIVDEALA--MGGCD-TLKQVIVY 226 A+ L ++ D+GA A+ V ++ K + +K IV ++ +G TL ++V Sbjct: 109 ARELEHQLKDSGAEAIFVLENFACTLQKVVAHTNIKHIVVASMGELLGALKGTLVNLVVR 168 Query: 227 KRTGAELNWVEGRDLWLSDVMAN-QAATCEPEWVDAEHPLFILYTSGSTGKPKGVQHASA 285 W + S ++ + T +P + + F+ YT G+TG KG Sbjct: 169 HVKKLVPAWSLPNAITFSQALSQARGKTLQPVTLTHDDIAFLQYTGGTTGVAKGAVLTHR 228 Query: 286 GYLLWAVLTMKWTFDI----KPDD--VYWCTADIGWITGHTYIAYGPLAVGATQIVFEGV 339 + + +W KP D V+ C + I T GA ++ + Sbjct: 229 NLVANVLQAEEWLTPALKAGKPIDQLVFVCALPLYHIFALTVCNMLGTREGALNLL---I 285 Query: 340 PTYPNAGRFWDMVARHKATIFYTAPTAIRSLIKAADADEKVHPKQYDLSTLRILGSVGEP 399 P + F +A++K F T +L+ AD + D S R G Sbjct: 286 PNPRDIPGFVKELAKYKVNTFPAVNTLYNALLNNADF------ARLDFSGYRCCVGGGMA 339 Query: 400 INPEAWMWYYKNVGRENCPIVDTFWQTETGGHMISPLPGATP----QVPGSCTLPLPGIT 455 + + K G CPI++ + +ET SP+ A P + G+ LPLP Sbjct: 340 VQKSVADKWKKLTG---CPIIEGYGLSET-----SPIASANPCTIDEYTGTIGLPLPSTE 391 Query: 456 AAIVDETGHDLPNGNGGILVVKRPWPSMIRTIWNDPERFKKSYFPEELGGNIYLAGDGAV 515 AI+D+ G +P G G + ++ P ++ W P+ K P+ + GD V Sbjct: 392 FAILDDDGQPVPLGQPGEIAIR--GPQVMPGYWQRPDETAKVMTPD----GFFKTGDIGV 445 Query: 516 RNKETGYFTITGRIDDVLNVSGHRMGTMEIESALVANSIVAEAAVVGKPDETTGESICAF 575 + E GY I R D++ VSG + E+E + + V E A VG PDE TGE++ F Sbjct: 446 MD-ERGYTKIVDRKKDMILVSGFNVYPNEVEGVVAQHPGVLECACVGVPDEHTGEAVKLF 504 Query: 576 VVLKRPRPTGEEAKQIAKELRDWVAKEIGPIAKPKEIRFGDNLPKTRSGKIMRRLLR 632 VV K + A +L D+ ++ KPK I F D LPKT GKI+RR LR Sbjct: 505 VVRK-------DKTLSAAQLMDYCKEQFTGYKKPKYIEFRDELPKTNVGKILRRELR 554 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,265,593 Number of extensions: 102868 Number of successful extensions: 309 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 282 Number of HSP's successfully gapped: 11 Length of query: 660 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 569 Effective length of database: 1,188,985 Effective search space: 676532465 Effective search space used: 676532465 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)