BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1375.p (1340 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310866|ref|YP_003774958.1| signal peptide protein [Herbasp... 2016 0.0 >gi|300310866|ref|YP_003774958.1| signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 1300 Score = 2016 bits (5224), Expect = 0.0, Method: Compositional matrix adjust. Identities = 1017/1292 (78%), Positives = 1166/1292 (90%), Gaps = 5/1292 (0%) Query: 50 THTESGARLLWNGAVKALGGKLSGQLLGGTVADGLRLRALHYQDDKRILDIDRIDARWHL 109 T TESGARLLWN AVK LGGKLSGQLLGGTVADGL LRA+HYQD +RILDIDR+DA W L Sbjct: 13 TQTESGARLLWNTAVKTLGGKLSGQLLGGTVADGLHLRAVHYQDGQRILDIDRLDASWRL 72 Query: 110 ALLRRSLRLDYLRVGQVQLNQQPSPSEPSTLPASLEIPLALEIGEISLERLNLTQGTSST 169 +LL+R L YLR G VQLNQQPSPSEPS+LPASL +PLALEIGEISL++ +LTQGTS+T Sbjct: 73 SLLKRRFSLAYLRAGTVQLNQQPSPSEPSSLPASLSLPLALEIGEISLDKFSLTQGTSTT 132 Query: 170 ELSRLKLHGNSDGVQHTLVLDSLTTPYGDATALLHLNGKRPFAIGGGLELTGAYQHGQLK 229 ELS+L+LHG+SDGVQHTLVLDSL TPYG ATAL+HLNGK PF IGGGLEL G YQHG+ K Sbjct: 133 ELSKLRLHGSSDGVQHTLVLDSLVTPYGQATALVHLNGKAPFDIGGGLELGGVYQHGEFK 192 Query: 230 EQYQLAAQLSGSLQELGIALTAGGDRLKGSADILATPLASVPLKRAKIDLQHINPQAFNA 289 EQYQLAAQLSGSLQELG+AL A GD+LKG ADI+ATP A+VPLKRA++DLQH+NPQAF+A Sbjct: 193 EQYQLAAQLSGSLQELGVALKASGDKLKGDADIIATPFAAVPLKRARVDLQHLNPQAFHA 252 Query: 290 AAPAADLHLRADLAPPAGLTADAPLQVSGRIDIDNAHPGRLDEQRLPLLSASADLKLDLQ 349 APAADL +RADLAP AG ADAPL+V+GRI+I+NA PG LD+Q LPLLSASADL+LDLQ Sbjct: 253 TAPAADLSVRADLAPSAGQAADAPLKVAGRIEINNARPGSLDQQLLPLLSASADLQLDLQ 312 Query: 350 QQTLSRLDIRLLRDARIEGSGQFRPGSNEPGKQEPLSGQFKLRVSDLDLSQLHRQVRPTR 409 QQ L+RL+IRL R+ RIEG+GQF+PGS +P + P++G+ LRVS+LDLSQ+H QVRPTR Sbjct: 313 QQALTRLEIRLPRNGRIEGAGQFQPGSQDPAQPTPMAGRLALRVSELDLSQIHHQVRPTR 372 Query: 410 LSGPVDLSLAPEAQQILLTLQDSALKLHVDSMIGHDRVQVRQASLTAGKSRLETTGTLQT 469 LSGPV+ +L P+A+Q+LLTLQDSAL+LH+DS + D V++R+ASLTAGKSRLE G+++T Sbjct: 373 LSGPVEATLGPDARQLLLTLQDSALRLHLDSTLRADTVELREASLTAGKSRLEAAGSMKT 432 Query: 470 TGAMDYELKGRLLDFDPSAVLQAV-APPAAPARKGRKSSAPVSKPIAARINMDFDAAGAL 528 GAMDY KGRL+DFDP+AVLQ V AP AA AR+G K+ A I ARIN+DF+AAG L Sbjct: 433 VGAMDYAFKGRLVDFDPTAVLQTVTAPTAASARRGGKTVAA----IPARINLDFNAAGLL 488 Query: 529 SPELRLKLGFAIHDSSYGELPMSGRGDLNLVAMRLLPSRAHLQIAGNTLDLDGSFGAPSD 588 SPELRLKLGF IHDSSYG+LPM+GRGD++L MRLLPS+AHLQ+AGNTLDL+GSFGA +D Sbjct: 489 SPELRLKLGFTIHDSSYGDLPMAGRGDIDLAGMRLLPSQAHLQVAGNTLDLNGSFGAAND 548 Query: 589 KLKLKIDAPQLAKLGYGLSGLLRVDGILAGTRQRPNLLASFQAEQLSFGAHKLHHLAGQS 648 KLKLKIDAPQLA+LGYGLSGLLR+DG L GTRQRP+L AS+QAEQL FG HKL HLAGQ+ Sbjct: 549 KLKLKIDAPQLARLGYGLSGLLRLDGQLGGTRQRPSLQASYQAEQLGFGPHKLQHLAGQA 608 Query: 649 ELHSNLAAGVASPDNRLALNVEGDGYSGPQAELAHLAIKLDGTYAKHALSLQAKGKLLDQ 708 E+HSNLAAGVASP+NRLAL +EGDGYSGPQAELA LA+KLDGTYAKH+LSL+A GKLL++ Sbjct: 609 EIHSNLAAGVASPENRLALTLEGDGYSGPQAELAQLAVKLDGTYAKHSLSLKANGKLLNR 668 Query: 709 SLDLNLAAHGQLTEKTRGHYGWAGSLDQFSNQGLPRIAMASPLALEASADGIEAGSTRIE 768 L L LAAHGQ+TEKTRG YGW GS+DQ SN G PRIAM PLAL+AS + +EAGSTRIE Sbjct: 669 PLALTLAAHGQVTEKTRGQYGWNGSIDQLSNDGFPRIAMLHPLALQASPEAVEAGSTRIE 728 Query: 769 IDRTALELKRLSYHQGRLASAGQVKALDVGRILELVQQLTGTPPPVKTDLVLDSDWDFTL 828 IDRTAL+L+ LS+ QGR+ASAG+V+ALDVGR+LEL QQL PP+KTDLVLDSDWDF+L Sbjct: 729 IDRTALDLRHLSWRQGRIASAGEVRALDVGRLLELAQQLGAAAPPMKTDLVLDSDWDFSL 788 Query: 829 AETASGYLQIARKSGDVRVTTSTGEATLGLSELQLRSQFQGEQMRVSGRVNAARVGTLEL 888 AE+A GY+QIAR+SGD+R+ TS GEATLGLSEL+LRS FQGEQMR+SGRV+A+R+GTL L Sbjct: 789 AESARGYVQIARQSGDLRLVTSNGEATLGLSELKLRSDFQGEQMRLSGRVDASRIGTLAL 848 Query: 889 DGEIGLQRVDQVLALDAASPLNLSAKLDIPQVKRMGDLIGPQVNLDGKLAANLNAAGTLE 948 G+IGLQRVDQ+L L SPL L+A+L++P++KR+GDLIGPQV+L+GKL+A+LN AGTL Sbjct: 849 GGQIGLQRVDQLLTLADGSPLALNARLELPELKRIGDLIGPQVSLNGKLSADLNVAGTLA 908 Query: 949 QPKLSGAINGDNLAVTEFDQGIQLKDGIVRIVMDSNVIDLRQIEFHGGSGTLRAGGKIQL 1008 QPKLSGA+NGD+LAVT+FDQGIQLKDGIVRIVMDSNVIDLRQIEFHGG GTLRAGGKIQL Sbjct: 909 QPKLSGAVNGDDLAVTQFDQGIQLKDGIVRIVMDSNVIDLRQIEFHGGRGTLRAGGKIQL 968 Query: 1009 GADNPDLNASLTADHLELFASPDRRLMLSGEGKISNVNEQLRVDGKFTVDRALFDLPKSS 1068 GADNPDLNA+LTA+ LELFASPDRRLMLSG+GKISNVNEQL VDGKFTVD ALFDLPKSS Sbjct: 969 GADNPDLNATLTAEQLELFASPDRRLMLSGQGKISNVNEQLHVDGKFTVDHALFDLPKSS 1028 Query: 1069 APKLGDDVVIISRSGKNRVGAAASSQQKLEAATEKPASKFAPIINLAIDLGNDFRFRGSG 1128 APKLGDDVVIISRSGKN+VG ASS+Q+LEAATEKPASKFAP IN+A+DLGNDFRFRGSG Sbjct: 1029 APKLGDDVVIISRSGKNQVGGQASSKQRLEAATEKPASKFAPFINIAVDLGNDFRFRGSG 1088 Query: 1129 ADLLLRGDMNVRSEPLSPLRATGTINVAEGSYEAFGTKLNIERGIINFTGPLSNPNLNIL 1188 ADL LRG+M VRSEPLSP+RA+GTINVAEGSYEAFGTKLNIERGIINF GP++NPN+NIL Sbjct: 1089 ADLRLRGEMAVRSEPLSPMRASGTINVAEGSYEAFGTKLNIERGIINFQGPIANPNINIL 1148 Query: 1189 AMRRNQDVDAGVSVTGNVNQPRVQLVSEPNVPDDEKLSWMMFGHGSDSSNIGQRTASSQA 1248 AMRRNQDV+AGVSVTGN NQPRVQLVSEPNVPDDEKLSWMMFGHG+D+S+IGQRTASSQA Sbjct: 1149 AMRRNQDVEAGVSVTGNANQPRVQLVSEPNVPDDEKLSWMMFGHGTDNSSIGQRTASSQA 1208 Query: 1249 LALVGNMAGKRITKDIGLDQFSIGSSDSGLTDDQVVNLGKAITQKITLGYEQSLQGAASI 1308 LALVGNM GKRI KDIGLDQFSIGSS+SGLT+DQVVNLGKAIT+KITLGYEQSLQGAAS+ Sbjct: 1209 LALVGNMGGKRIAKDIGLDQFSIGSSESGLTNDQVVNLGKAITEKITLGYEQSLQGAASV 1268 Query: 1309 AKATWQISRRWSVVARAGTVNGLNILFSQRYD 1340 AKATWQISRRWSVVARAGT+NGLNILF++RYD Sbjct: 1269 AKATWQISRRWSVVARAGTINGLNILFNRRYD 1300 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.133 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 3,864,128 Number of extensions: 165356 Number of successful extensions: 534 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 529 Number of HSP's successfully gapped: 1 Length of query: 1340 Length of database: 1,619,870 Length adjustment: 97 Effective length of query: 1243 Effective length of database: 1,160,575 Effective search space: 1442594725 Effective search space used: 1442594725 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 137 (57.4 bits)