BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1365.p (446 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310856|ref|YP_003774948.1| major facilitator superfamily p... 824 0.0 gi|300311040|ref|YP_003775132.1| major facilitator superfamily p... 504 e-144 gi|300313188|ref|YP_003777280.1| major facilitator superfamily p... 325 3e-90 gi|300312683|ref|YP_003776775.1| major facilitator superfamily p... 241 1e-64 gi|300312017|ref|YP_003776109.1| multidrug resistance B (translo... 122 8e-29 >gi|300310856|ref|YP_003774948.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 446 Score = 824 bits (2129), Expect = 0.0, Method: Compositional matrix adjust. Identities = 413/446 (92%), Positives = 432/446 (96%) Query: 1 MDSTVISTSLPPLALDMKVDPISLKLALTSYLVSLAVFIPISGWMADRFGSLTIFRAAIC 60 MDSTVISTSLP LALDM VDPISLKLALTSYLVSLAVFIPISGWMADRFGSLTIFR+AIC Sbjct: 1 MDSTVISTSLPALALDMNVDPISLKLALTSYLVSLAVFIPISGWMADRFGSLTIFRSAIC 60 Query: 61 VFMLGSILCGMSSTLPQFVAARFLQGIGGAMMVPVGRLVILRSVEKVNLVKALSYLTIPA 120 VFMLGSILCG+SSTLPQFVAARFLQGIGGAMMVPVGRLVILRSVEKVNLVKALSYLTIPA Sbjct: 61 VFMLGSILCGVSSTLPQFVAARFLQGIGGAMMVPVGRLVILRSVEKVNLVKALSYLTIPA 120 Query: 121 LFGPVLGPPLGGFITTYFHWRWIFFINIPISVLGLYLATRFIPDLKAEQVSPLDFKGFVL 180 LFGPVLGPPLGGFITTYFHWRWIFFINIPIS+LGLYLATRFIPDLKAEQV+PLD KGF+L Sbjct: 121 LFGPVLGPPLGGFITTYFHWRWIFFINIPISILGLYLATRFIPDLKAEQVAPLDLKGFLL 180 Query: 181 SALGCSLLMMGLATAGRHLVSGDVALACVVAGTLFLAGYVWHARRATAPLIDLALLKIPT 240 SALGCSLLMMGLATAGRHLVSG+VAL CV+AG +FLA YVWHARRA+APLIDLALLKIPT Sbjct: 181 SALGCSLLMMGLATAGRHLVSGEVALTCVLAGLVFLAAYVWHARRASAPLIDLALLKIPT 240 Query: 241 LRAGVFGGSLYRVGIGAIPFLLPLMLQLGFGYTPFHSGLLTCASAAGAMFMKTIAARFLR 300 LRAGVFGGS YRVGIGAIPFLLPLMLQLGFGYTPFHSG+LTCASAAGAMFMKTIAARFLR Sbjct: 241 LRAGVFGGSFYRVGIGAIPFLLPLMLQLGFGYTPFHSGMLTCASAAGAMFMKTIAARFLR 300 Query: 301 RFGFRRVLLVNGALGVLGLCAIATFTPTTPYLLMVLILLIGGCFRSLQFTSLNAISYADV 360 R+GFRRVLLVNGALGVLGL AIATFTP TPYLLMV+ILLIGGCFRSLQFTSLNAISYADV Sbjct: 301 RYGFRRVLLVNGALGVLGLWAIATFTPQTPYLLMVVILLIGGCFRSLQFTSLNAISYADV 360 Query: 361 DNLRLSQATSMASVAQQLSIGMGVVLAGFALQMSNEIQGHSELVAQDFWPAFLVVAAVAS 420 D LRLSQATSMASVAQQLSIGMGVVLAGFALQMSNEIQGH+ LVAQDFWPAFLVV AVA+ Sbjct: 361 DKLRLSQATSMASVAQQLSIGMGVVLAGFALQMSNEIQGHATLVAQDFWPAFLVVGAVAA 420 Query: 421 LALLMLRQLPPNAGAEMAGQEENKPA 446 ALLMLRQLP +AGAEMAG+++N+PA Sbjct: 421 CALLMLRQLPHDAGAEMAGRDDNRPA 446 >gi|300311040|ref|YP_003775132.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 441 Score = 504 bits (1298), Expect = e-144, Method: Compositional matrix adjust. Identities = 249/438 (56%), Positives = 328/438 (74%) Query: 1 MDSTVISTSLPPLALDMKVDPISLKLALTSYLVSLAVFIPISGWMADRFGSLTIFRAAIC 60 MD+TV++TSLP LA D+ DPI+LKLA+T+Y+ +L VFIPISGWMAD+ G+ +F AA+ Sbjct: 1 MDATVLATSLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMADKLGARKVFSAAMI 60 Query: 61 VFMLGSILCGMSSTLPQFVAARFLQGIGGAMMVPVGRLVILRSVEKVNLVKALSYLTIPA 120 VFM GSILC +S +L FVAARFLQGIGGAMMVPVGR++I RSV+K LVKA+SYLT+PA Sbjct: 61 VFMTGSILCAVSGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDKSELVKAVSYLTLPA 120 Query: 121 LFGPVLGPPLGGFITTYFHWRWIFFINIPISVLGLYLATRFIPDLKAEQVSPLDFKGFVL 180 L GPV+GP LGGFITTYFHWRW+F INIP LGLYLA RFI + K + +PLD KGFVL Sbjct: 121 LLGPVVGPLLGGFITTYFHWRWVFLINIPFCFLGLYLARRFIQNFKEDNPAPLDVKGFVL 180 Query: 181 SALGCSLLMMGLATAGRHLVSGDVALACVVAGTLFLAGYVWHARRATAPLIDLALLKIPT 240 +++G +++M+GL+ G HLV G +A G + L Y H+ + PL+D LL+IPT Sbjct: 181 TSVGAAMIMLGLSLFGSHLVDGATPVAMCSIGAIALVMYYRHSTQIEFPLLDFRLLRIPT 240 Query: 241 LRAGVFGGSLYRVGIGAIPFLLPLMLQLGFGYTPFHSGLLTCASAAGAMFMKTIAARFLR 300 L A V GGSL+R+G+GA+PFLLPL LQ G G T F +G +TCASA G++F++ +A R LR Sbjct: 241 LHASVVGGSLFRIGLGAVPFLLPLALQEGLGMTAFQAGSITCASAFGSLFIRLLAGRVLR 300 Query: 301 RFGFRRVLLVNGALGVLGLCAIATFTPTTPYLLMVLILLIGGCFRSLQFTSLNAISYADV 360 R+GFR VL+VN + L A F+ TPYLL+V I+L+GG F +LQFT LN+I+YAD+ Sbjct: 301 RYGFRTVLMVNAVFAGIALAAYGIFSIHTPYLLIVSIVLMGGVFPALQFTCLNSIAYADI 360 Query: 361 DNLRLSQATSMASVAQQLSIGMGVVLAGFALQMSNEIQGHSELVAQDFWPAFLVVAAVAS 420 D+ + TS+ASV QQLS+G+GV +AG LQ+SN +QGH+ +V DFWPAFLV+ + Sbjct: 361 DSRDAGRVTSLASVIQQLSLGLGVTVAGIVLQLSNHLQGHATIVEADFWPAFLVIGLFSV 420 Query: 421 LALLMLRQLPPNAGAEMA 438 ++ + +LP NAG E++ Sbjct: 421 ASVPISGKLPHNAGLELS 438 >gi|300313188|ref|YP_003777280.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 325 bits (834), Expect = 3e-90, Method: Compositional matrix adjust. Identities = 186/451 (41%), Positives = 272/451 (60%), Gaps = 6/451 (1%) Query: 1 MDSTVISTSLPPLALDMKVDPISLKLALTSYLVSLAVFIPISGWMADRFGSLTIFRAAIC 60 +D+T+I+T+LP +A V P L L +T+Y+++LAVFIPISGW+ADRFGS T+F AI Sbjct: 45 LDTTIIATALPQMAQSFGVGPNELSLGMTAYMLTLAVFIPISGWVADRFGSRTVFGMAIA 104 Query: 61 VFMLGSILCGMSSTLPQFVAARFLQGIGGAMMVPVGRLVILRSVEKVNLVKALSYLTIPA 120 +F L SILCG S + +F AAR LQG+GGAMMVPVGR++++RS K L+KA++ +T PA Sbjct: 105 IFTLASILCGFSQNVWEFTAARVLQGLGGAMMVPVGRMIVVRSTGKDRLMKAIATITWPA 164 Query: 121 LFGPVLGPPLGGFITTYFHWRWIFFINIPISVLGLYLATRFIPDLKAEQVSPLDFKGFVL 180 + PV+GP +GGFITTY W WIF +N+P + + + PLD GF+L Sbjct: 165 IVAPVVGPSIGGFITTYASWHWIFLMNVPFGLAAFTAVMMVVRNEHGVSKRPLDLVGFLL 224 Query: 181 SALGCSLLMMGLATAGRHLVSGDVALACVVAGTLFLAGYVWHARRATAPLIDLALLKIPT 240 S + L+ G A + VAL V G + G H +R PL+D + L+IPT Sbjct: 225 SGAALTSLLYGTEIASHMESNIKVALGFVALGVVLGYGAWRHMQRIEHPLLDFSNLRIPT 284 Query: 241 LRAGVFGGSLYRVGIGAIPFLLPLMLQLGFGYTPFHSGLLTCASAAGAMFMKTIAARFLR 300 V GSL R+G+ A+P+L+PLM Q+GFG T F SGLL A+A G + MK R L+ Sbjct: 285 YSVTVLTGSLTRIGVDAVPYLMPLMFQIGFGLTAFQSGLLLLATALGNLGMKAFTTRILQ 344 Query: 301 RFGFRRVLLVNGALGVLGLCAIATFTPTTPYLLMVLILLIGGCFRSLQFTSLNAISYADV 360 RFGFR V + N A+ + A TP TP +++ ++ G RS+QF++L ++YAD+ Sbjct: 345 RFGFRSVAVNNAAIAGGLIIACGLLTPATPLPVILGVIFCYGLSRSMQFSTLATLAYADI 404 Query: 361 DNLRLSQATSMASVAQQLSIGMGVVLAGFALQMSNEIQGH------SELVAQDFWPAFLV 414 + R S A+++ SVAQQ++IGMG+ AL+++ ++G + DF AF + Sbjct: 405 SDARKSSASTLWSVAQQMTIGMGIAFGALALRIAAALRGEAIDASGAHFTLGDFQWAFAM 464 Query: 415 VAAVASLALLMLRQLPPNAGAEMAGQEENKP 445 V ++++ R L +AG+ + G P Sbjct: 465 AGVVTLISVIGYRGLARDAGSNIGGGMRAAP 495 >gi|300312683|ref|YP_003776775.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 241 bits (614), Expect = 1e-64, Method: Compositional matrix adjust. Identities = 150/435 (34%), Positives = 237/435 (54%), Gaps = 9/435 (2%) Query: 1 MDSTVISTSLPPLALDMKVDPISLKLALTSYLVSLAVFIPISGWMADRFGSLTIFRAAIC 60 +D+T+++T+LP +A + +++ + +Y +++A P SGW+ADRFG+ ++ AAI Sbjct: 67 LDTTIVNTALPAMARSLGEPVLNMHPVVVAYTLTMASLTPASGWLADRFGTRRMYFAAIL 126 Query: 61 VFMLGSILCGMSSTLPQFVAARFLQGIGGAMMVPVGRLVILRSVEKVNLVKALSYLTIPA 120 +F LGS+LC M+S+LP +AAR +QG+GG+M++PVGRL +LR+V + AL+ ++I Sbjct: 127 LFSLGSLLCAMASSLPMLLAARVVQGLGGSMLLPVGRLAVLRAVPGAQYIAALASISIAG 186 Query: 121 LFGPVLGPPLGGFITTYFHWRWIFFINIPISVLGLYLATRFIPDLKAEQVSPLDFKGFVL 180 GPV+GP LGG++ W WIF IN+P+ VLG+ F+PD D G VL Sbjct: 187 QLGPVVGPVLGGWLVESLSWHWIFLINLPVGVLGMGAVLYFLPDDSLAGTPRFDLLGCVL 246 Query: 181 SALGCSLLMMGLATA---GRHLVSGDVALACVVAGTLFLAGYVWHARRATAPLIDLALLK 237 L ++ LA H +AL V A T+ GY HA PL L L Sbjct: 247 --LSACMVAFSLALDVPFDSHRTGWSIALFVVAALTVL--GYWRHASVHPRPLFRLGLFG 302 Query: 238 IPTLRAGVFGGSLYRVGIGAIPFLLPLMLQLGFGYTPFHSGLLTCASAAGAMFMKTIAAR 297 P G+ G + R+G A+PFLLPL+LQ+ GY+P H+G++ +A K A Sbjct: 303 EPNFSIGLAGNLVCRIGSSAVPFLLPLLLQVRLGYSPLHAGMVLLPAAIAGTIAKPWIAP 362 Query: 298 FLRRFGFRRVLLVNGALGVLGLCAIATFTPTTPYLLMVLILLIGGCFRSLQFTSLNAISY 357 ++RFG+ R LLVN A+ + + A TP P ++ L I G S+QF ++N+++ Sbjct: 363 LVKRFGYDRFLLVNTAIVGAAIMSFALITPDWPLTAAIVQLAIFGASNSMQFAAMNSVTL 422 Query: 358 ADVDNLRLSQATSMASVAQQLSIGMGVVLAGFALQMSNEIQGHSELVAQDFWPAFLVVAA 417 +D S+ S+ Q L+IG+GV + G + + G + F F +V A Sbjct: 423 KGLDKRDAGSGNSLFSMVQMLAIGLGVTIGGTMVNLFKSEVGTAT--NASFQITFAIVGA 480 Query: 418 VASLALLMLRQLPPN 432 + + + R++ P Sbjct: 481 ITLASAWVFRKMDPR 495 >gi|300312017|ref|YP_003776109.1| multidrug resistance B (translocase) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 524 Score = 122 bits (305), Expect = 8e-29, Method: Compositional matrix adjust. Identities = 95/315 (30%), Positives = 156/315 (49%), Gaps = 14/315 (4%) Query: 1 MDSTVISTSLPPLALDMKVDPISLKLALTSYLVSLAVFIPISGWMADRFGSLTIFRAAIC 60 +DS++ + ++P ++ ++ V +TS+ + A+ IP++GW+ R G++ +F ++ Sbjct: 38 LDSSIANVAIPTISGNLGVSVDEGTWVITSFAAANAISIPLTGWLTQRLGAVRLFVTSVL 97 Query: 61 VFMLGSILCGMSSTLPQFVAARFLQGIGGAMMVPVGRLVILRSVEKVNLVKALSYLTIPA 120 +F+L S LCG++ TLP +AAR LQG+ MVP+ + ++L + K AL+ + A Sbjct: 98 LFVLASWLCGLAPTLPILLAARVLQGLVAGPMVPLSQSLLLGAYPKSKSSFALALWGMTA 157 Query: 121 LFGPVLGPPLGGFITTYFHWRWIFFINIPISVL--GLYLA---TRFIPDLKAEQVSPLDF 175 + PV GP LGG+IT + W WIF+INIP+ ++ G+ LA +R P K P+D Sbjct: 158 VVAPVAGPALGGWITDSYTWPWIFYINIPVGLIAAGVTLAIYRSRETPTHKL----PIDK 213 Query: 176 KGFVL--SALGCSLLMMGLATAGRHLVSGDVALACVVAGTLFLAGYVWHARRATAPLIDL 233 G L + + +M+ S + ++A F VW P++DL Sbjct: 214 IGLALLVTWVAAFQIMLDKGKDLDWFASPTIVTLGIIALIAFAYFVVWELNN-DHPVVDL 272 Query: 234 ALLKIPTLRAGVFGGSL-YRVGIGAIPFLLPLMLQLGFGYTPFHSGLLTCASAAGAMFMK 292 L G S+ Y V G + +LP LQ GY SGL T A+ + Sbjct: 273 RLFGRRNFAGGTIAISIGYGVFFGNL-VILPQWLQQYLGYRSIDSGLSTAPIGIFAVILM 331 Query: 293 TIAARFLRRFGFRRV 307 + RFL R+V Sbjct: 332 PLIGRFLPLVDARKV 346 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.329 0.142 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,157,146 Number of extensions: 43517 Number of successful extensions: 291 Number of sequences better than 1.0e-08: 16 Number of HSP's gapped: 278 Number of HSP's successfully gapped: 16 Length of query: 446 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 358 Effective length of database: 1,203,190 Effective search space: 430742020 Effective search space used: 430742020 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)