BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1363.p (513 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310854|ref|YP_003774946.1| NodT family protein [Herbaspiri... 840 0.0 gi|300311796|ref|YP_003775888.1| outer membrane channel lipoprot... 402 e-113 gi|300309656|ref|YP_003773748.1| outer membrane drug efflux prot... 325 6e-90 gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipo... 280 3e-76 gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbas... 204 1e-53 >gi|300310854|ref|YP_003774946.1| NodT family protein [Herbaspirillum seropedicae SmR1] Length = 507 Score = 840 bits (2171), Expect = 0.0, Method: Compositional matrix adjust. Identities = 463/509 (90%), Positives = 484/509 (95%), Gaps = 2/509 (0%) Query: 5 MTQPHPFKLKPLLAVLLASCALAACSVGPDYVRPQMDTGPAVFKEGQNWKPAQPRDLAAD 64 MTQ FKLKPLLA+L+AS ALAACSVGPDYVRPQMDTGPA+FKEGQNWKPAQPRDL AD Sbjct: 1 MTQQDLFKLKPLLAMLVASLALAACSVGPDYVRPQMDTGPAIFKEGQNWKPAQPRDLVAD 60 Query: 65 GKWWEMFGDPQLNALIAQVDISNQNLALAEANFRSAVALVQSSRSAYSPTLSASAGASRA 124 GKWWEMFGDPQLNAL+AQVDISNQNLALAEANFRSAVALVQSSRSAYSPTLSASAGASR Sbjct: 61 GKWWEMFGDPQLNALVAQVDISNQNLALAEANFRSAVALVQSSRSAYSPTLSASAGASRG 120 Query: 125 SSGSAGSSSGNGGSTNGASSGGVRNSYTFSLTAAWEPDLWGKVRRDVESSQTSAQASAAD 184 SSGSAG+SS N S + A S VRNSYTFSLTAAWE D+WGKVRRDVESSQ SAQASAAD Sbjct: 121 SSGSAGASSSNAASGSSAGS--VRNSYTFSLTAAWEADIWGKVRRDVESSQASAQASAAD 178 Query: 185 LAAARLSAQAQVAQDYLQLRVLDAQQQLLEDTVQAYQRSLQVTQNQYAAGMVAKSDVIQA 244 LAAARLSAQAQVAQ+YLQLRVLDAQQQLLE+TVQAY+RSLQV QNQYAAGMVAKSDVIQA Sbjct: 179 LAAARLSAQAQVAQNYLQLRVLDAQQQLLEETVQAYERSLQVAQNQYAAGMVAKSDVIQA 238 Query: 245 QTQLKSAQASALDNGVTRAQVEHAIALLVGKTPAEFSLPRRPLTAVAPPVPQGIPSMLLE 304 QTQLKSAQASALDNGVTRAQVEHAIALLVGKTPA+F+LPR PL AVAPPVPQG+PSMLLE Sbjct: 239 QTQLKSAQASALDNGVTRAQVEHAIALLVGKTPAQFALPRAPLAAVAPPVPQGVPSMLLE 298 Query: 305 RRPDVAAAERRVAAANAKIGVAKAAYFPSLTLSASGGYQSSSFSDWFTLPGRVWSLGPQL 364 RRPDVAAAERRVA+ANAKIGVAKAAYFPSLTLSASGGYQSS+F+DWFTLPGRVWSLGPQL Sbjct: 299 RRPDVAAAERRVASANAKIGVAKAAYFPSLTLSASGGYQSSTFADWFTLPGRVWSLGPQL 358 Query: 365 AQTIFDGGARAAATDQAIANYDGTVATYKQTVLTAFQDVEDNLAALRILEQEAAVQHEAL 424 AQTIFDGGARAAATDQAIA YDGTVATYKQTVLTAFQ+VEDNLAALRILEQEAAVQ EAL Sbjct: 359 AQTIFDGGARAAATDQAIATYDGTVATYKQTVLTAFQEVEDNLAALRILEQEAAVQGEAL 418 Query: 425 LAARQAVTLVTNQYKAGTVSYVNVITAQATALSAELTSLNILNRRMAASVLLAKALGGGW 484 AARQAVTLV NQYKAGTVSYVNVITAQATALSAEL+SLNILNRRMAASVLLAKALGGGW Sbjct: 419 QAARQAVTLVNNQYKAGTVSYVNVITAQATALSAELSSLNILNRRMAASVLLAKALGGGW 478 Query: 485 DEHSLDDLGPLEQQTADKAKALKENAALR 513 DE SLDDLG L+QQ+A+KAKALKENAALR Sbjct: 479 DERSLDDLGSLQQQSAEKAKALKENAALR 507 >gi|300311796|ref|YP_003775888.1| outer membrane channel lipoprotein [Herbaspirillum seropedicae SmR1] Length = 510 Score = 402 bits (1033), Expect = e-113, Method: Compositional matrix adjust. Identities = 250/494 (50%), Positives = 327/494 (66%), Gaps = 29/494 (5%) Query: 6 TQPHPFKLKPLLAVLLASCALAACSVGPDYVRPQMDTGPAVFKEGQNWKPAQPRDLAADG 65 TQ PF+ + L+ + L C+VGPDY RP +D P +KE WK A PR + Sbjct: 24 TQYLPFRATKGMTALVLAALLGGCAVGPDYARPALDL-PQAYKETGPWKLAAPRQIDDHQ 82 Query: 66 KWWEMFGDPQLNALIAQVDISNQNLALAEANFRSAVALVQSSRSAYSPTLSASAGASRAS 125 WWE +GDP LNAL+ Q + +NQ + A A +R A A + +R++ PT+ A AGASRA Sbjct: 83 PWWEAYGDPVLNALMDQANAANQTIQQAAAQYRQAQATAEVARASLWPTIGAQAGASRAQ 142 Query: 126 SGSAGSSSGNGGSTNGASSGGVRNSYTFSLTAAWEPDLWGKVRRDVESSQTSAQASAADL 185 + +TNG G NSY+ L A+WE DLWG++RR E+ + +AQASAA L Sbjct: 143 T-----------NTNGVQRLG--NSYSVGLNASWEADLWGRIRRGAEAGEANAQASAASL 189 Query: 186 AAARLSAQAQVAQDYLQLRVLDAQQQLLEDTVQAYQRSLQVTQNQYAAGMVAKSDVIQAQ 245 AAARLS QA +AQDYLQLRV D Q+ L TV AY RSLQ++ +QY AG +SDV QA+ Sbjct: 190 AAARLSIQATLAQDYLQLRVTDLQKDLYRRTVAAYTRSLQLSTHQYEAGTALRSDVAQAE 249 Query: 246 TQLKSAQASALDNGVTRAQVEHAIALLVGKTPAEFSLPRRP---------------LTAV 290 TQL++AQA +D TR Q+EHAIA+L+GK PA+FSLP P L Sbjct: 250 TQLRAAQAQLIDLDDTRNQLEHAIAMLIGKAPAQFSLPALPPAAASDSAIDANAARLQGS 309 Query: 291 APPVPQGIPSMLLERRPDVAAAERRVAAANAKIGVAKAAYFPSLTLSASGGYQSSSFSDW 350 P +P G+PS LLERRPD+A AER AAANA IGVA+AAYFP+LTLSASGGY S +F++ Sbjct: 310 LPQIPVGLPSELLERRPDIANAERLAAAANANIGVARAAYFPTLTLSASGGYNSLAFANL 369 Query: 351 FTLPGRVWSLGPQLAQTIFDGGARAAATDQAIANYDGTVATYKQTVLTAFQDVEDNLAAL 410 F P RVWSLG LA+++FDGGAR+A D A+A YD VA YKQTVL Q+VED L+ L Sbjct: 370 FNTPSRVWSLGSALAESLFDGGARSARNDAAVAAYDAAVAQYKQTVLGGLQEVEDKLSTL 429 Query: 411 RILEQEAAVQHEALLAARQAVTLVTNQYKAGTVSYVNVITAQATALSAELTSLNILNRRM 470 R+L+QE+ VQ +A+ +A+ A L QY+AGTV+Y++V+T QA +L+ + ++ +L R++ Sbjct: 430 RVLDQESTVQAQAVQSAQLAERLAMRQYEAGTVTYLSVVTTQAASLTNQRNAVTLLGRQL 489 Query: 471 AASVLLAKALGGGW 484 ASV L KA GGGW Sbjct: 490 VASVALIKATGGGW 503 >gi|300309656|ref|YP_003773748.1| outer membrane drug efflux protein [Herbaspirillum seropedicae SmR1] Length = 504 Score = 325 bits (833), Expect = 6e-90, Method: Compositional matrix adjust. Identities = 207/472 (43%), Positives = 282/472 (59%), Gaps = 25/472 (5%) Query: 29 CSVGPDYVRPQMDTGPAVFKE----------GQNWKPAQPRDLAADGKWWEMFGDPQLNA 78 CSV P Y RP +DT PA FKE G WK AQP + A G+WW++FGD +LNA Sbjct: 31 CSVAPTYERPSVDT-PAAFKEAAPATQTGENGSQWKTAQPAEDIARGEWWKIFGDEKLNA 89 Query: 79 LIAQVDISNQNLALAEANFRSAVALVQSSRSAYSPTLSASAGASR--ASSGSAGSSSGNG 136 L +NQ+L A A A AL + +R+ Y P + A GA+R S S G + Sbjct: 90 LEDTAQQANQDLKAAAARLGQARALTRDARAGYFPQVDAGVGATRQRPSPASQGLPADAD 149 Query: 137 GSTNGASSGGVRNSYTFSLTAAWEPDLWGKVRRDVESSQTSAQASAADLAAARLSAQAQV 196 N Y + ++E DL+G+V V+++ AQ S A + L+ QA V Sbjct: 150 TRPN--------TLYRAQVNVSYEVDLFGRVASTVDAATADAQRSEALFHSVLLALQADV 201 Query: 197 AQDYLQLRVLDAQQQLLEDTVQAYQRSLQVTQNQYAAGMVAKSDVIQAQTQLKSAQASAL 256 AQ Y +R LDA L TV Q SL++ Q ++ G +++ DV +A+ +L SAQ+ AL Sbjct: 202 AQQYFLVRELDAAAALYRGTVDLRQESLRLVQRRFDEGDISEVDVARARAELASAQSEAL 261 Query: 257 DNGVTRAQVEHAIALLVGKTPAEFSLPRRPLTAVAPPVPQGIPSMLLERRPDVAAAERRV 316 RA EHA+A+L+G+TPAEFSLP +PLT +A VP G+PS LLERRPD+AAAER + Sbjct: 262 GIARQRATAEHALAILLGRTPAEFSLPPQPLTRLAVTVPPGLPSALLERRPDIAAAERAM 321 Query: 317 AAANAKIGVAKAAYFPSLTLSASGGYQSSSFSDWFTLPGRVWSLGP----QLAQTIFDGG 372 AAANA++GVAK+A+FPSL L+ GY+S+ D F R + LGP L+ +FDGG Sbjct: 322 AAANARVGVAKSAFFPSLNLTGGLGYESAQLGDLFNWSSRTFLLGPLVGTALSMPLFDGG 381 Query: 373 ARAAATDQAIANYDGTVATYKQTVLTAFQDVEDNLAALRILEQEAAVQHEALLAARQAVT 432 R A D+A A+Y+ VA+Y+ TVL AF++VEDNLA LRIL+Q+ Q EA+ A+ +A Sbjct: 382 RRQAGVDRARASYEEQVASYRGTVLNAFREVEDNLANLRILDQQNRAQDEAVAASARAAK 441 Query: 433 LVTNQYKAGTVSYVNVITAQATALSAELTSLNILNRRMAASVLLAKALGGGW 484 L QY+ G VSY +VI A T L + S+ + R ++V L +ALGGGW Sbjct: 442 LSHTQYREGAVSYFSVIDADRTVLQQQRASVTLDGERARSTVNLIRALGGGW 493 >gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 486 Score = 280 bits (715), Expect = 3e-76, Method: Compositional matrix adjust. Identities = 183/461 (39%), Positives = 270/461 (58%), Gaps = 7/461 (1%) Query: 29 CSVGPDYVRPQMDTGPAVFKEGQNWKPAQPRDLAADGKWWEMFGDPQLNALIAQVDISNQ 88 C+ GPDY RPQ+D PA + W+ QP D G WWE FGD QLNAL Q NQ Sbjct: 19 CASGPDYTRPQVDM-PAAWTSEAPWRSMQPADATPRGPWWERFGDAQLNALQQQALAGNQ 77 Query: 89 NLALAEANFRSAVALVQSSRSAYSPTLSASAGASRASSGSAGSSSGNGGSTNGASSGGVR 148 LA+A A A A + + +A +P ++ +A A+R S SA N S N ++ V+ Sbjct: 78 TLAIATARLAQARAQLNVTGAAQTPQVNLNARAAR-SRISANRPLTNYNSPNFST---VQ 133 Query: 149 NSYTFSLTAAWEPDLWGKVRRDVESSQTSAQASAADLAAARLSAQAQVAQDYLQLRVLDA 208 N + + ++E D +G+V+R VE++ +A+ SAADL RL A++A +Y LR LD Sbjct: 134 NDFALGASVSYEVDFFGRVQRSVEAAGAAAEQSAADLENTRLLLTAELATNYFNLRELDV 193 Query: 209 QQQLLEDTVQAYQRSLQVTQNQYAAGMVAKSDVIQAQTQLKSAQASALDNGVTRAQVEHA 268 + ++ + +++L++ ++ G + D+ Q Q L S G RAQ EHA Sbjct: 194 ELDVVRRAIALQRQALELATARHDLGATSGLDMAQQQALLDSTLTQIDLLGRQRAQYEHA 253 Query: 269 IALLVGKTPAEFSLPRRPLTAVAPP-VPQGIPSMLLERRPDVAAAERRVAAANAKIGVAK 327 IA L G FS+ LT + PP +P G+PS +LERRPD+AAAER +AAANA+IGVA Sbjct: 254 IATLTGTPAPAFSITPS-LTPITPPAIPVGVPSDVLERRPDIAAAERAMAAANAQIGVAS 312 Query: 328 AAYFPSLTLSASGGYQSSSFSDWFTLPGRVWSLGPQLAQTIFDGGARAAATDQAIANYDG 387 AAY+PS L S G S +++ F P +WSLG +Q++FDGG A D + A Y+ Sbjct: 313 AAYYPSFMLQPSYGVDSRNWATLFNAPSLLWSLGVSASQSLFDGGRLRAGVDFSQAGYEA 372 Query: 388 TVATYKQTVLTAFQDVEDNLAALRILEQEAAVQHEALLAARQAVTLVTNQYKAGTVSYVN 447 TVA+Y++TVLTA Q+VED + L LE+ A A+ +AR+ + + +++Y+ G Y++ Sbjct: 373 TVASYRRTVLTAMQEVEDGITGLAALERAYAQSQLAIASARRVLEIASSRYEGGATPYLD 432 Query: 448 VITAQATALSAELTSLNILNRRMAASVLLAKALGGGWDEHS 488 VITAQ + L++E + ++ +R+ SV L KALGG W + Sbjct: 433 VITAQQSLLNSERQAAQLMGQRLLVSVFLIKALGGDWQSRT 473 >gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 483 Score = 204 bits (519), Expect = 1e-53, Method: Compositional matrix adjust. Identities = 159/489 (32%), Positives = 251/489 (51%), Gaps = 19/489 (3%) Query: 5 MTQPHPFKLKPLLAVLLASCALAACSVGPDYVRPQMDTGPAVFKEGQNWKPAQPRDLAAD 64 ++ P +L + A L+ + L CS+ P Y RPQ + + Q +L Sbjct: 4 LSMPRSPRLLHMCAALVMAGVLGGCSLAPTYERPQAPVESSYPADAAGRAELQAVNLG-- 61 Query: 65 GKWWEMFGDPQLNALIAQVDISNQNLALAEANFRSAVALVQSSRSAYSPTLSASAGASRA 124 W + F D +L LIA +N++L A N A A Q +R+ P L+ S A+R Sbjct: 62 --WRQFFPDQRLQQLIAAALENNRDLRTAVLNIEEARAQYQIARADLLPNLNLSGNAAR- 118 Query: 125 SSGSAGSSSGNGGSTNGASSGGVRNSYTFSLTA-AWEPDLWGKVRRDVESSQTSAQASAA 183 + +A S S G T + NSY L A+E D +G+VR +++ S ++ Sbjct: 119 -TRTAASQSLTGQPT-------ISNSYQVGLAVPAYELDFFGRVRNLKDAALASYLSTEE 170 Query: 184 DLAAARLSAQAQVAQDYLQLRVLDAQQQLLEDTVQAYQRSLQVTQNQYAAGMVAKSDVIQ 243 +A++S AQVAQ YL R Q +L ++T+++ Q +L + Q + G D Sbjct: 171 AQKSAQISLVAQVAQAYLAERSYAEQLKLADETLKSRQSNLDLAQKRLDVGASTALDFRL 230 Query: 244 AQTQLKSAQASALDNGVTRAQVEHAIALLVGKTPAEFSLPRRPLT--AVAPPVPQGIPSM 301 QT ++SA+ + RAQ E+A+ LLVGK E P + L+ + +P G+PS Sbjct: 231 TQTLVESARVAKTQLERQRAQAENALTLLVGKKIGELP-PAQLLSDEKIVTDIPAGLPSD 289 Query: 302 LLERRPDVAAAERRVAAANAKIGVAKAAYFPSLTLSASGGYQSSSFSDWFTLPGRVWSLG 361 LL RPD+ +AE+ + AANA IG A+AA+FP ++L+ + G SS+ F WS G Sbjct: 290 LLTNRPDIRSAEQSLLAANANIGAARAAFFPRISLTGNFGSASSTLGGLFESGSGAWSFG 349 Query: 362 PQLAQTIFDGGARAAATDQAIANYDGTVATYKQTVLTAFQDVEDNLAALRILEQEAAVQH 421 P L+ IFD G +A D A + V TY++ + TAF++V D L A L+++ Q Sbjct: 350 PSLSLPIFDFGRNSANLDLAEVRKNKAVVTYEKAIQTAFREVADALVARGSLDEQVKAQ- 408 Query: 422 EALLAARQAVTLVTN-QYKAGTVSYVNVITAQATALSAELTSLNILNRRMAASVLLAKAL 480 EA LAA+Q +T+ ++K G S ++ + AQ SA+ + R+ ++ L KAL Sbjct: 409 EAFLAAQQERLKLTDLRFKNGIASSLDQLDAQRDLFSAQQALIQARQLRLNNAIDLYKAL 468 Query: 481 GGGWDEHSL 489 GGG +E+++ Sbjct: 469 GGGLNENTV 477 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.313 0.125 0.348 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,175,409 Number of extensions: 39010 Number of successful extensions: 239 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 182 Number of HSP's successfully gapped: 17 Length of query: 513 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 424 Effective length of database: 1,198,455 Effective search space: 508144920 Effective search space used: 508144920 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 133 (55.8 bits)