BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1357.p (459 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310848|ref|YP_003774940.1| two component sensor histidine ... 830 0.0 gi|300313666|ref|YP_003777758.1| two component sensor histidine ... 241 1e-64 gi|300313829|ref|YP_003777921.1| sensor histidine kinase [Herbas... 170 3e-43 gi|300311468|ref|YP_003775560.1| oxidative stress resistance two... 129 3e-31 gi|300313679|ref|YP_003777771.1| transmembrane sensor histidine ... 108 7e-25 >gi|300310848|ref|YP_003774940.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 465 Score = 830 bits (2143), Expect = 0.0, Method: Compositional matrix adjust. Identities = 415/465 (89%), Positives = 432/465 (92%), Gaps = 6/465 (1%) Query: 1 MTRFLGSIGNRIFVLLLAGIVMAVFATNWLAGNERRNTLRELRFQHLADRVEQFVRAVDE 60 M FLGSIGNRIF LLL GIV+AVFATNWLAGNERRNTLRELRFQHLADRVEQ V+AVD Sbjct: 1 MKNFLGSIGNRIFFLLLGGIVVAVFATNWLAGNERRNTLRELRFQHLADRVEQIVQAVDN 60 Query: 61 LPLSQRSIVLQAAANFGFEARMVEDMNEAID---GPNASNTPLVEMLQARLGQDRKIAVQ 117 +P +QR IVLQAA+NFGFEARMVEDMN+AI P+ S +PL EMLQARLG+DRKIAVQ Sbjct: 61 IPPAQRPIVLQAASNFGFEARMVEDMNDAIGPDASPSPSTSPLTEMLQARLGEDRKIAVQ 120 Query: 118 RENECPPLRPDDARARHHDTCRVIYISLHDHALMRLRLHQPGEAPALRGGSGPGGP---F 174 RE+ECPPLR DDARA DTCRVIYISLHD ALMRLRLHQPGE PALR +GPGGP F Sbjct: 121 RESECPPLRTDDARAPRRDTCRVIYISLHDQALMRLRLHQPGEPPALRARAGPGGPASLF 180 Query: 175 GLQYILLFLVLIAMLAWLVARMATRPIRQLAQAASRLGSDIDHAPLAETGPTEIRQAAHA 234 GLQYILLFLVLIAMLAWLVARMATRPIRQLAQAASRLGSDIDHAPLAETGPTEIRQAAHA Sbjct: 181 GLQYILLFLVLIAMLAWLVARMATRPIRQLAQAASRLGSDIDHAPLAETGPTEIRQAAHA 240 Query: 235 FNAMQARIRRQIQHRTHMLAAITHDLQTPLTRMRLRLEKVADSQLQQKLIDDLGVMHGMV 294 FNAMQARIRRQIQHRTHMLAAITHDLQTPLTRMRLRLEKV+D LQQKLIDDLGVMHGMV Sbjct: 241 FNAMQARIRRQIQHRTHMLAAITHDLQTPLTRMRLRLEKVSDPGLQQKLIDDLGVMHGMV 300 Query: 295 REGLDLARSMDSSEKIQALDIDSLLDSVCADAADSGQDVSLQGSTRAFVMAQPGALRRCL 354 REGLDLARSMDSSEKIQALDIDSLLDSVCADAAD+GQDV+LQGSTRAFVMAQPGALRRCL Sbjct: 301 REGLDLARSMDSSEKIQALDIDSLLDSVCADAADAGQDVALQGSTRAFVMAQPGALRRCL 360 Query: 355 TNLIDNACKYGKVARVTIALEQQKIAIRIRDQGPGIPEAELEAVFEPFYRLEASRSRDTG 414 TNLIDNACKYGK ARV+IALEQQKIAIRIRDQGPGIPE+ELEAVFEPFYRLEASRSRDTG Sbjct: 361 TNLIDNACKYGKSARVSIALEQQKIAIRIRDQGPGIPESELEAVFEPFYRLEASRSRDTG 420 Query: 415 GTGLGLTIALNIAENHHGQLRLRNLSEGGLEVLLELPTMTPGKPR 459 GTGLGLTIALNIAENHHGQLRLRNL+EGGLEVLLELP MTP K R Sbjct: 421 GTGLGLTIALNIAENHHGQLRLRNLAEGGLEVLLELPPMTPAKNR 465 >gi|300313666|ref|YP_003777758.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 479 Score = 241 bits (614), Expect = 1e-64, Method: Compositional matrix adjust. Identities = 156/471 (33%), Positives = 254/471 (53%), Gaps = 35/471 (7%) Query: 7 SIGNRIFVLLLAGIVMAVFATNWLAGNERRNTLRELRFQHLADRVEQFVRAVDELPLSQR 66 ++ R+ ++L+AG++ A T + + R T E+ + A + V +D+ P +QR Sbjct: 17 TLSGRLALILVAGMLAAQMLTGTIWYDMRYGTAMEMPTRLAAAHLADAVLILDDAPATQR 76 Query: 67 SIVLQAAANFGFEARM---VEDMNEAIDGPNASNTPLV---------------------- 101 + + F + V+ + A+ G +++P Sbjct: 77 QALAHSLTRAQFVLSLPDVVDRADLAVPGLAPASSPTTHADQGVVDLFGTVLQQHAGPRP 136 Query: 102 -EMLQARLGQDRKIAVQRENECPPLRPDDARARHHDTCRVIYISLHDHALMRLRLHQPGE 160 +MLQ LG D + A + LR + R + ++L D + ++ + + G+ Sbjct: 137 WKMLQ--LGLDDQHANAESSWWTLLRDEFPHGRFR-----VLVTLKDGSWLQADIEE-GQ 188 Query: 161 APALRGGSGPGGPFGLQYILLFLVLIAMLAWLVARMATRPIRQLAQAASRLGSDIDHAPL 220 A + ++ L + + ++AW+ R+A +P++ +A+AA +LG +I APL Sbjct: 189 AGMSTAPHSAAFDYFMRIYALRIFAVVLIAWMAVRLAMKPLQVMAEAAEKLGGNIRSAPL 248 Query: 221 AETGPTEIRQAAHAFNAMQARIRRQIQHRTHMLAAITHDLQTPLTRMRLRLEKVADSQLQ 280 A GP E+ AA AFNAMQ RI I RT LAA++HDL++P+TRM+LR E + D + + Sbjct: 249 ATDGPLEVSTAAQAFNAMQRRIIDNIAERTRFLAAVSHDLRSPITRMKLRTEMLPDERGK 308 Query: 281 QKLIDDLGVMHGMVREGLDLARSMDSSEKIQALDIDSLLDSVCADAADSGQDVSLQGSTR 340 +K DL M ++ L++ + +D E+ Q +D+DSLL+S+ D A+ G +V+L G Sbjct: 309 EKFRKDLDEMDALISATLNVVQDIDVQERPQLIDLDSLLESLRTDQAEVGGEVTLSGHA- 367 Query: 341 AFVMAQPGALRRCLTNLIDNACKYGKVARVTIALEQQKIAIRIRDQGPGIPEAELEAVFE 400 A + P +LRRCL NL++NA +YG+ ARV + + ++ IR+ D GPGIPEA LE V E Sbjct: 368 APLQGYPRSLRRCLQNLVENAVRYGQRARVQLQDDADQVCIRVDDDGPGIPEALLERVQE 427 Query: 401 PFYRLEASRSRDTGGTGLGLTIALNIAENHHGQLRLRNLSEGGLEVLLELP 451 PFYRLE SR+ TGG GLGL+IA + + H G ++L N GGL V + +P Sbjct: 428 PFYRLERSRNAGTGGFGLGLSIAATVVQAHRGVMKLVNRPGGGLRVEILIP 478 >gi|300313829|ref|YP_003777921.1| sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 478 Score = 170 bits (430), Expect = 3e-43, Method: Compositional matrix adjust. Identities = 100/263 (38%), Positives = 150/263 (57%), Gaps = 5/263 (1%) Query: 193 VARMATRPIRQLAQAASRLGSDIDHAPLAETGPTEIRQAAHAFNAMQARIRRQIQHRTHM 252 V R TRP+ LA AA G L ETG E + A AF+ M+ RI+R ++ R + Sbjct: 215 VVRWTTRPLAALADAAEAFGKGETVPSLPETGSREYVKTARAFSGMRERIKRYLEDRERL 274 Query: 253 LAAITHDLQTPLTRMRLRLEKVADSQLQQKLIDDLGVMHGMVREGLDLARSMDSSEKIQA 312 +I+HDL+TP+TR++LR E + D ++ DDL + MV+ L + D E Sbjct: 275 FVSISHDLRTPITRLKLRTELMDDDAMRTDFHDDLDELDMMVKGALQSVKDSDIHENRTE 334 Query: 313 LDIDSLLDSVCADAADSGQDVSLQGSTRAFVMAQPGALRRCLTNLIDNACKYGKVARVTI 372 + +D+L+ + DA +G +++ S V+A+P AL+R + NL DNA YG+ ++I Sbjct: 335 VRLDALVLRMIRDARLAGHEIAFTPSG-INVIAKPLALKRAIGNLFDNALYYGQKVEISI 393 Query: 373 ----ALEQQKIAIRIRDQGPGIPEAELEAVFEPFYRLEASRSRDTGGTGLGLTIALNIAE 428 E + I IRD GPG+PE + +F+P+ RLE R++++GG GLGL I+ NI + Sbjct: 394 RPSPGAEGDLVEIEIRDHGPGVPEDAMGTLFQPYVRLEHGRAQNSGGMGLGLGISRNIVQ 453 Query: 429 NHHGQLRLRNLSEGGLEVLLELP 451 H G+L LRN EGGL + LP Sbjct: 454 AHGGELLLRNHPEGGLMATIILP 476 >gi|300311468|ref|YP_003775560.1| oxidative stress resistance two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 455 Score = 129 bits (325), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 97/289 (33%), Positives = 148/289 (51%), Gaps = 15/289 (5%) Query: 174 FGLQYILLFLVLIAMLAWLVARMATRPIRQLAQAASRLGSDIDHAPLAETGPTEIRQAAH 233 G I LFL LI A ++ + +P+ +L AA + PL E+GPTEI +A Sbjct: 163 LGWGSIALFLSLIG--AVFISTLINQPLARLTAAARAIAKGRQPEPLPESGPTEIEEANR 220 Query: 234 AFNAMQARIRRQIQHRTHMLAAITHDLQTPLTRMRLRLEKVADS-QLQQKLIDDLGVMHG 292 +FN M A + R R +LA I+HDL+TPL RM+L +E S + + + DL M Sbjct: 221 SFNQMVADLNRIESDRALILAGISHDLRTPLARMQLEVEMANLSDEARDGMHSDLAQMDS 280 Query: 293 MVREGLDLARSMDSSEKIQALDIDSLLDSVCADAAD-SGQDVSLQGSTRAFVMAQPGALR 351 ++ + LD A+ D+S ++ +D+ +L V +A+ S V Q + V L Sbjct: 281 IIGQFLDYAKPFDTS-SLETIDLADVLQDVAGNASRLSDIRVHTQITPDTQVAGNGTELA 339 Query: 352 RCLTNLIDNACKYGKVARVTIA-------LEQQKIAIRIRDQGPGIPEAELEAVFEPFYR 404 R +NL++NA +YGK A +E ++ + + D GPG+P E E + PF R Sbjct: 340 RVFSNLVENARRYGKTEGTDCAEIDIRSRIEGHQVVVEVADHGPGVPVNECERLLRPFTR 399 Query: 405 LEASRSRDTGGTGLGLTIALNIAENHHGQLRLRNLSE--GGLEVLLELP 451 L+ +R + G+GLGL I I H+G+L L+ E GL V + LP Sbjct: 400 LDTARGQ-ANGSGLGLAIVNRIVLKHNGKLLLKGHEETRSGLLVQITLP 447 >gi|300313679|ref|YP_003777771.1| transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 444 Score = 108 bits (271), Expect = 7e-25, Method: Compositional matrix adjust. Identities = 96/283 (33%), Positives = 139/283 (49%), Gaps = 29/283 (10%) Query: 186 IAMLAWLVARMATRPIRQLA-QAASRLGSDIDHAPLAETG-PTEIRQAAHAFNAMQARIR 243 I +L+W+ P+R+ A + A+R + + PLA+ G P EI+ HA N + AR++ Sbjct: 153 IGVLSWVAVSRGLAPVRKAAAEVAARDMNSLQ--PLADVGMPQEIQPLTHALNDLLARLK 210 Query: 244 RQIQHRTHMLAAITHDLQTPLTRMRLRL---EKVADSQLQQKLIDDLG-----VMHGMVR 295 + + +A H+L+TPLT +RL+ E+ D + ++ DL H +V Sbjct: 211 HATETQRAFVADAAHELRTPLTALRLQTQLAERAVDEEERRAAFADLKNGLERTTH-LVH 269 Query: 296 EGLDLARSMDSSEKIQALDIDSLLDSVCAD----AADSGQDVSL--QGST-RAF-VMAQP 347 + L LAR + +D+ +LL SV D A G D+ L QG R V Sbjct: 270 QLLTLARQEPDAMSFTQVDLRALLGSVVGDLSSVAEHGGIDLGLSDQGDDGRPLRVTGNA 329 Query: 348 GALRRCLTNLIDNACKY---GKVARVTIALEQQ-KIAIRIRDQGPGIPEAELEAVFEPFY 403 ALR NL+DNA +Y G V V + L + ++D GPGI E+EL VF+ FY Sbjct: 330 DALRILFNNLVDNALRYTPAGGVVDVGVDLSTAGYCTVVVQDSGPGIAESELPRVFDRFY 389 Query: 404 RL----EASRSRDTGGTGLGLTIALNIAENHHGQLRLRNLSEG 442 R E S SR G+GLGL I IA+ H + LRN G Sbjct: 390 RAQHSQEGSGSRAAFGSGLGLAIVRQIADLHRVPVELRNTGHG 432 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.137 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,270,323 Number of extensions: 48574 Number of successful extensions: 308 Number of sequences better than 1.0e-08: 36 Number of HSP's gapped: 274 Number of HSP's successfully gapped: 36 Length of query: 459 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 371 Effective length of database: 1,203,190 Effective search space: 446383490 Effective search space used: 446383490 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 132 (55.5 bits)