BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1344.p (588 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310835|ref|YP_003774927.1| acyl-CoA dehydrogenase [Herbasp... 1150 0.0 gi|300309596|ref|YP_003773688.1| acyl-CoA dehydrogenase [Herbasp... 471 e-134 gi|300313303|ref|YP_003777395.1| acyl-CoA dehydrogenase [Herbasp... 392 e-110 gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbasp... 128 1e-30 gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbasp... 107 3e-24 >gi|300310835|ref|YP_003774927.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 594 Score = 1150 bits (2976), Expect = 0.0, Method: Compositional matrix adjust. Identities = 562/588 (95%), Positives = 581/588 (98%) Query: 1 VKDMLFVMNELAGLAAVNALPGCEDATPETAEAILEEKAKFCSEVIAPLNHSADKEPSSW 60 VKDMLFVMNELAGLAAVNALPGCEDATPETAEAILEE AKFCSEV+APLNHSADKEPSSW Sbjct: 7 VKDMLFVMNELAGLAAVNALPGCEDATPETAEAILEENAKFCSEVVAPLNHSADKEPSSW 66 Query: 61 ADGKVTTSPGFKQAFRQFGEAGWQGVQHPVEFGGQGLPKLLATPCIEMLNSANLSFALCP 120 ADGKVTTSPGFK+AFRQFGEAGWQGVQHPVEFGGQGLPKLLATPCIEMLNSANLSFALCP Sbjct: 67 ADGKVTTSPGFKEAFRQFGEAGWQGVQHPVEFGGQGLPKLLATPCIEMLNSANLSFALCP 126 Query: 121 LLTDGTIEALMTAGSQAQKETFIANFISGKWTGTMNLTEPQAGSDLALVRTRAVPEGDGT 180 LLTDGTIEALMTAG+Q QKETFIANFISGKWTGTMNLTEPQAGSDLALVRTRAVPEGDGT Sbjct: 127 LLTDGTIEALMTAGTQQQKETFIANFISGKWTGTMNLTEPQAGSDLALVRTRAVPEGDGT 186 Query: 181 YKIFGTKIFITYGEHDMAENIVHLVLARTPNAPEGVKGISLFIVPKFLINADGSLGARND 240 YK+FGTKIFITYGEHDMAENIVHLVLARTPNAPEGVKGISLFIVPKFL+NADGSLGARND Sbjct: 187 YKLFGTKIFITYGEHDMAENIVHLVLARTPNAPEGVKGISLFIVPKFLVNADGSLGARND 246 Query: 241 VHCVSIEHKLGIKASPTAVLQFGDHGGAIGTLVGEENRGLEYMFIMMNAARFAVGIQGLS 300 VHCVSIEHKLGIKASPTAVLQFGDHGGAIGTLVGEENRGLEYMFIMMNAARFAVGIQGLS Sbjct: 247 VHCVSIEHKLGIKASPTAVLQFGDHGGAIGTLVGEENRGLEYMFIMMNAARFAVGIQGLS 306 Query: 301 VSERSYQKAVAYARDRIQSRDLAGSPGPVAIIHQPDVRRMLMSMRAQIEAARALAYVAAA 360 VSER+YQKAVAYARDRIQSRDLAGSPGPVAIIHQPDVRRMLMSMRAQIEAARAL+YVAAA Sbjct: 307 VSERAYQKAVAYARDRIQSRDLAGSPGPVAIIHQPDVRRMLMSMRAQIEAARALSYVAAA 366 Query: 361 TSDAAHFSNDEATRKSNQAFYEFLVPIVKGWITEMSVDVTSTGVQVHGGMGFIEETGAAQ 420 TSDAAHFS+DEATRKSNQAFYE+LVPIVKGWITEMS+DVTSTGVQVHGGMGFIEETGAAQ Sbjct: 367 TSDAAHFSDDEATRKSNQAFYEYLVPIVKGWITEMSIDVTSTGVQVHGGMGFIEETGAAQ 426 Query: 421 YYRDARILPIYEGTTAIQANDLVGRKTARDGGAVAKGILAQVRQTVSELQGDAQLQPIAA 480 YYRDARILPIYEGTTAIQANDLVGRKTARDGGAVAKGILAQVR+TVSELQGDAQLQPIAA Sbjct: 427 YYRDARILPIYEGTTAIQANDLVGRKTARDGGAVAKGILAQVRKTVSELQGDAQLQPIAA 486 Query: 481 RLAEAADALEQVVEYVVGNLKSDIKAVFAGSVPYLKMAGVVLGGWQMARAAAIAAAKLKA 540 RL EAADALEQVV+YVV N+KSDIKAVFAGSVPYLKMAGVVLGGWQMARAAA++AAKLKA Sbjct: 487 RLGEAADALEQVVDYVVANVKSDIKAVFAGSVPYLKMAGVVLGGWQMARAAAVSAAKLKA 546 Query: 541 GEGDDKFYAAKLATARFYADQILPQALAHRTAIVEGGQAVLALAEDQF 588 GEGD KFY AKLATARFYADQILPQALA+RTAIV+GG AV+ALAE+QF Sbjct: 547 GEGDGKFYEAKLATARFYADQILPQALAYRTAIVDGGAAVMALAEEQF 594 >gi|300309596|ref|YP_003773688.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 596 Score = 471 bits (1213), Expect = e-134, Method: Compositional matrix adjust. Identities = 257/573 (44%), Positives = 351/573 (61%), Gaps = 15/573 (2%) Query: 1 VKDMLFVMNELAGLAAV-NALPGCEDATPETAEAILEEKAKFCSEVIAPLNHSADKEPSS 59 ++DM FV++EL + V LP D E + +LEE KF S+V+ PLNHS D+E Sbjct: 8 LRDMQFVLHELLNVEEVFKELPPHADTNRELVDQVLEEGGKFASQVLFPLNHSGDREGCH 67 Query: 60 W--ADGKVTTSPGFKQAFRQFGEAGWQGVQHPVEFGGQGLPKLLATPCIEMLNSANLSFA 117 + VTT GFKQA++Q+ EAGW + EFGGQGLP + EMLNS+N ++ Sbjct: 68 YDRESKTVTTPKGFKQAYQQYVEAGWAALSCDPEFGGQGLPLTINNSFYEMLNSSNQAWT 127 Query: 118 LCPLLTDGTIEALMTAGSQAQKETFIANFISGKWTGTMNLTEPQAGSDLALVRTRAVPEG 177 + P L+ G E L GS+ QK+ ++ +SG+WTGTM LTE G+DL L+R++A P+ Sbjct: 128 MYPGLSHGAYECLHAHGSEEQKQLYLPKLVSGEWTGTMCLTEAHCGTDLGLLRSKAEPQA 187 Query: 178 DGTYKIFGTKIFITYGEHDMAENIVHLVLARTPNAPEGVKGISLFIVPKFLINADGSLGA 237 DG+YKI G KIFI+ GE+D++ NI+HLVLAR P+APEG KGISLF+VPKFL NADGSLGA Sbjct: 188 DGSYKITGNKIFISAGENDVSANIIHLVLARLPDAPEGSKGISLFLVPKFLPNADGSLGA 247 Query: 238 RNDVHCVSIEHKLGIKASPTAVLQFGDHGGAIGTLVGEENRGLEYMFIMMNAARFAVGIQ 297 RN + C +IE K+GI + T + GA G L+G+ ++GL+ MF+ MNAAR VG+Q Sbjct: 248 RNPIFCGAIEEKMGIHGNATCQMNL---DGATGWLIGQPHKGLQAMFVFMNAARLGVGMQ 304 Query: 298 GLSVSERSYQKAVAYARDRIQSRDLAG-----SPGPVAIIHQPDVRRMLMSMRAQIEAAR 352 GL ++E +YQ A+ YA+DR+Q R L+G P I+H PDVRRML++ +A E R Sbjct: 305 GLGLTEVAYQNALVYAKDRLQMRSLSGIKAADKPADPIIVH-PDVRRMLLTAKAFAEGGR 363 Query: 353 ALAYVAAATSDAAHFSNDEATRKSNQAFYEFLVPIVKGWITEMSVDVTSTGVQVHGGMGF 412 A A D DE RK + L P+VK ++T+ + TS +QV+GG G+ Sbjct: 364 AFTSYVALELDKELTHPDEQVRKDSADIVALLTPVVKAFLTDNAWMATSECLQVYGGHGY 423 Query: 413 IEETGAAQYYRDARILPIYEGTTAIQANDLVGRKTARDGGAVAKGILAQVRQTVSELQGD 472 I E G QY RDARI IYEGT IQ+ DL+GRK D GA K AQ++ V E D Sbjct: 424 IAEWGMEQYVRDARINMIYEGTNTIQSLDLLGRKILMDNGAKLKKFGAQIQAFVEEHGTD 483 Query: 473 AQLQPIAARLAEAADALEQVVEYVVGNLKSDIKAVFAGSVPYLKMAGVVLGGWQMARAAA 532 + LA+ D + ++ + ++ A +VPYL++ G ++ + AR A Sbjct: 484 ESMSEFITPLADIGDKVTKLTMEIGMKSFANQDEAGAAAVPYLRVVGHLVFSYLFARMAK 543 Query: 533 IAAAKLKAGEGDDKFYAAKLATARFYADQILPQ 565 IA K +G D FYAAKLATARFY ++LP+ Sbjct: 544 IALEKQDSG---DSFYAAKLATARFYFARLLPE 573 >gi|300313303|ref|YP_003777395.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 591 Score = 392 bits (1006), Expect = e-110, Method: Compositional matrix adjust. Identities = 231/559 (41%), Positives = 307/559 (54%), Gaps = 16/559 (2%) Query: 1 VKDMLFVMNE-LAGLAAVNALPGCEDATPETAEAILEEKAKFCSEVIAPLNHSADKEPSS 59 ++D FV++E L + ALP + + +LEE +F ++V+APLN D+E Sbjct: 9 LRDFAFVLDEVLQAPQVLRALPAHAEIDGDLMMQVLEEAGRFAADVVAPLNGIGDREGCR 68 Query: 60 WADGKVTTSPGFKQAFRQFGEAGWQGVQHPVEFGGQGLPKLLATPCIEMLNSANLSFALC 119 + +G VTT PGF++A+ QF GW + E GGQGLP +L EML++AN + + Sbjct: 69 FKNGTVTTPPGFERAYAQFCAGGWPALACAPEHGGQGLPHVLDCALYEMLSAANHGWTMY 128 Query: 120 PLLTDGTIEALMTAGSQAQKETFIANFISGKWTGTMNLTEPQAGSDLALVRTRAVPEGDG 179 P L G L GS A + ++ SG+W TM LTE QAGSDL +RTRAVP+ DG Sbjct: 129 PGLLHGAYACLAAHGSAALQARYLDKIASGEWLATMCLTEAQAGSDLGQLRTRAVPQDDG 188 Query: 180 TYKIFGTKIFITYGEHDMAENIVHLVLARTPNAPEGVKGISLFIVPKFLINADGSLGARN 239 +Y++ G KIFI+ GEHD+ NIVHLVLAR P+AP G KG+SLF+ P+ L + G+RN Sbjct: 189 SYRLSGDKIFISGGEHDLTPNIVHLVLARLPDAPAGSKGLSLFLAPRVLDD-----GSRN 243 Query: 240 DVHCVSIEHKLGIKASPTAVLQFGDHGGAIGTLVGEENRGLEYMFIMMNAARFAVGIQGL 299 VHC IEHK+GI S T L F A LVGE RGL MF+MMNAAR VG QGL Sbjct: 244 GVHCTGIEHKMGIHGSATCSLHFEQ---AQAWLVGEAGRGLAAMFVMMNAARLHVGAQGL 300 Query: 300 SVSERSYQKAVAYARDRIQSRDLAGSPGPVAIIHQPDVRRMLMSMRAQIEAARALAYVAA 359 ++E +YQ+A AYAR+R QSR GS G I+H P V+++LM R +IE AR L A Sbjct: 301 GLAEAAYQRASAYARERRQSR-APGSSGNDLIVHHPAVQKLLMEQRCRIEGARLLTCWAG 359 Query: 360 ATSDAAHFSNDEATRKSNQAFYEFLVPIVKGWITEMSVDVTSTGVQVHGGMGFIEETGAA 419 D A + A R + EF+ P++K ++T+ S +QV GG G++ ETG Sbjct: 360 LLLDLAEHDPEAALRSRHHERLEFITPVIKAFLTDQGFQCASRALQVFGGHGYVVETGIE 419 Query: 420 QYYRDARILPIYEGTTAIQANDLVGRKTARDGGAVAKGILAQVRQTVSELQGDAQLQPIA 479 Q+ RDARI IYEGT IQA D + RK D G L VR ++E G L A Sbjct: 420 QFMRDARITMIYEGTNEIQAIDFLMRKVLGDEGRRLDDFLGLVRADIAEASG---LGEQA 476 Query: 480 ARLAEAADALEQVVEYVVGNLKSDIKAVFAGSVPYLKMAGVVLGGWQMARAAAIAAAKLK 539 L EA D L + + + L++ G V GW RA A L Sbjct: 477 RLLGEAVDKLRIFASRIAAAAAQAPALPYYVADEMLRLCGHVALGWMWLRAGRRA---LS 533 Query: 540 AGEGDDKFYAAKLATARFY 558 A D +Y K A ++ Sbjct: 534 AMAQDPLWYGGKRDAACYH 552 >gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 128 bits (322), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 112/382 (29%), Positives = 173/382 (45%), Gaps = 45/382 (11%) Query: 73 QAFRQFGEAGWQGVQHPVEFGGQGLPKLLATPCIEML----NSANLSFALCPLLTDGTIE 128 +A ++ E G G+ P ++GG G+ + +E + + + ++ L G E Sbjct: 38 EALKELAELGAMGMCVPEQWGGAGMDYMSLVLALEEIAAGDGATSTIVSVQNSLACGITE 97 Query: 129 ALMTAGSQAQKETFIANFISGKWTGTMNLTEPQAGSDLALVRTRAVPEGDGTYKIFGTKI 188 GS QKE ++ G+ G LTEP GSD A +RTRA +GD + + GTK Sbjct: 98 KY---GSPTQKEQWLKPLARGEMLGCFCLTEPHTGSDAAAIRTRAERDGD-DFILNGTKQ 153 Query: 189 FITYGEHDMAENIVHLVLARTPNAPEGVKGISLFIVPKFLINADGSLGARNDVHCVSIEH 248 FIT G+H V +V A T + G KGIS F+VP + G + R+ E Sbjct: 154 FITSGKHAG----VAIVFAVTDRSA-GKKGISCFLVP---CDTPGFVVGRS-------EE 198 Query: 249 KLGIKASPTAVLQFGDHGGAIGTLVGEENRGLEYMFIMMNAARFAVGIQGLSVSERSYQK 308 K+G AS T + + L+G+E G + A R + Q + ++ +++ Sbjct: 199 KMGQHASDTVQIMLDNCRVPATALLGKEGEGYRIALSNLEAGRIGIAAQSVGMARAAFEA 258 Query: 309 AVAYARDRIQSRDLAGSPGPVAIIHQPDVRRMLMSMRAQIEAARALAYVAAATSDAAHFS 368 AV+YAR R+ G P II V L M ++AAR + + AA D Sbjct: 259 AVSYAR----QRESFGVP----IIEHQAVNFRLADMNTLLDAARLMVWRAAQLKDQGRPC 310 Query: 369 NDEATRKSNQAFYEFLVPIVKGWITEMSVDVTSTGVQVHGGMGFIEETGAAQYYRDARIL 428 EA+ + K + +E + + S +Q+HGG+G+ + + YRD RI Sbjct: 311 LKEAS-------------MAKMFASEAAEKIASDAIQIHGGVGYTSDFPVERIYRDVRIC 357 Query: 429 PIYEGTTAIQANDLVGRKTARD 450 IYEG IQ ++GR A D Sbjct: 358 QIYEGANDIQ-RLVIGRAIASD 378 >gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 107 bits (267), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 107/361 (29%), Positives = 166/361 (45%), Gaps = 41/361 (11%) Query: 79 GEAGWQGVQHPVEFGGQGLPKLLATPCIEMLNSANLSFALCPLLTDGTIEALMTAGSQAQ 138 GE G+ G+ P GG GL +L AT E L A S A + + + + G + Sbjct: 46 GELGFCGLYTPEADGGLGLSRLDATVVFEELARACTSTAAYLTIHNMVSWMIASWGQPSL 105 Query: 139 KETFIANFISGKWTGTMNLTEPQAGSDLALVRTRAVPEGDGTYKIFGTKIFIT-YGEHDM 197 K + +G+ G+ LTEP +GSD A ++T A E DG Y + G+K FI+ G D Sbjct: 106 KAQWCEQLAAGRKIGSYCLTEPGSGSDAASLKTTARLE-DGHYVLNGSKAFISGAGSTD- 163 Query: 198 AENIVHLVLARTPNAPEGVKGISLFIVPKFLINADG-SLGARNDVHCVSIEHKLGIKASP 256 V +V+ART EG +G+S +VP NA G S G + E K+G + P Sbjct: 164 ----VLVVMART--GGEGARGVSAIVVPG---NAPGVSYGKK--------ESKMGWNSQP 206 Query: 257 TAVLQFGDHGGAIGTLVGEENRGLEYMFIMMNAARFAVGIQGLSVSERSYQKAVAYARDR 316 T + F + L+G+E +G + ++ R + + ++ + A AY ++R Sbjct: 207 TRTISFDNVKVPADHLLGQEGQGFIFAMKGLDGGRINIATCSVGAAQAALDAAHAYMKER 266 Query: 317 IQSRDLAGSPGPVAIIHQPDVRRMLMSMRAQIEAARALAYVAAATSDAAHFSNDEATRKS 376 Q G P I ++ L M+ ++ AAR + +AA DA + EAT Sbjct: 267 KQF----GRP----IADFQALQFKLADMQTELVAARQMVRLAATKLDAG---SPEATT-- 313 Query: 377 NQAFYEFLVPIVKGWITEMSVDVTSTGVQVHGGMGFIEETGAAQYYRDARILPIYEGTTA 436 + K T++ V + +Q+HGG G+I E +Y+RD R+ I EGT Sbjct: 314 -------YCAMAKRLATDLGFRVCNEALQLHGGYGYIREYPLERYFRDVRVHQILEGTNE 366 Query: 437 I 437 I Sbjct: 367 I 367 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,786,829 Number of extensions: 74210 Number of successful extensions: 288 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 268 Number of HSP's successfully gapped: 9 Length of query: 588 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 498 Effective length of database: 1,193,720 Effective search space: 594472560 Effective search space used: 594472560 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)