BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1340.p (314 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310831|ref|YP_003774923.1| 2-ketogluconate kinase [Herbasp... 582 e-167 gi|300311076|ref|YP_003775168.1| sugar kinase,ribokinase family ... 102 3e-23 gi|300311742|ref|YP_003775834.1| transferase kinase [Herbaspiril... 95 9e-21 gi|300310353|ref|YP_003774445.1| ribokinase [Herbaspirillum sero... 65 6e-12 >gi|300310831|ref|YP_003774923.1| 2-ketogluconate kinase [Herbaspirillum seropedicae SmR1] Length = 313 Score = 582 bits (1500), Expect = e-167, Method: Compositional matrix adjust. Identities = 289/313 (92%), Positives = 301/313 (96%) Query: 1 MTMQLDVVTWGEALALLVADEVGPFEEVEKYTRRLAGAETNVAIGLARLGLKVGWASRVG 60 MTMQLDVVTWGEALALLVADEVGPFEEVEKYTRRLAGAETNVAIGLARLGLKVGWASRVG Sbjct: 1 MTMQLDVVTWGEALALLVADEVGPFEEVEKYTRRLAGAETNVAIGLARLGLKVGWASRVG 60 Query: 61 NDAFGRFIRKRVAQEGVDVSRVLTDMEFRTAIQLKAKAVDGADPAIEYYRKGSAASHLSL 120 NDAFGRFIR+RVAQEGV+VSRV+TDMEFRTAIQLKAKAV GADPAIEYYRKGSAASHLS+ Sbjct: 61 NDAFGRFIRQRVAQEGVEVSRVITDMEFRTAIQLKAKAVGGADPAIEYYRKGSAASHLSV 120 Query: 121 ADFDADYFGAARHLHATGIAPALSPSTMAFAHHAMEVMRAAGKTISFDPNLRPMLWPSKE 180 DFDA YFGAARHLHATGIAPALS +TMAFAH AM+ MR GKTISFDPNLRPMLWPS+E Sbjct: 121 DDFDAGYFGAARHLHATGIAPALSATTMAFAHQAMDFMRGQGKTISFDPNLRPMLWPSQE 180 Query: 181 VMAQQLNALAFKADWVLPGLSEGKILTGYEDPREIAGFYLERDVKMVVIKLGAEGAYWRT 240 VMAQQLNALAFKADWVLPGLSEGKILTG++D REIAGFYLER VK+VVIKLGAEGAYWR Sbjct: 181 VMAQQLNALAFKADWVLPGLSEGKILTGHDDAREIAGFYLERGVKLVVIKLGAEGAYWRN 240 Query: 241 GEGEGRVAGVPVKEVVDTVGAGDGFAVGVISGMLEGLPVAQAVMRGNRIGAFAIQVVGDM 300 G+GEGRVAGVPVKEVVDTVGAGDGFAVGVISGMLEGLPV QAVMRGNRIGAFAIQVVGDM Sbjct: 241 GQGEGRVAGVPVKEVVDTVGAGDGFAVGVISGMLEGLPVPQAVMRGNRIGAFAIQVVGDM 300 Query: 301 EGLPTRAELDAAS 313 EGLPTRAEL+AAS Sbjct: 301 EGLPTRAELEAAS 313 >gi|300311076|ref|YP_003775168.1| sugar kinase,ribokinase family protein [Herbaspirillum seropedicae SmR1] Length = 308 Score = 102 bits (255), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 100/309 (32%), Positives = 136/309 (44%), Gaps = 8/309 (2%) Query: 3 MQLDVVTWGEALALLVADEVGPFEEVEKYTRRLAGAETNVAIGLARLGLKVGWASRVGND 62 M ++ +GEA+ P + ++ Y G +N AI AR G + S VG+D Sbjct: 1 MSKTILAYGEAMVEFNQRIDAPRQFLQGY----GGDTSNFAIAAARQGAASAYLSAVGDD 56 Query: 63 AFGRFIRKRVAQEGVDVSRVLTDMEFRTAIQLKAKAVDGADPAIEYYRKGSAASHLSLAD 122 FG+ + +E VDV V T I D A Y R GSAAS Sbjct: 57 HFGQDLLALWQREEVDVRHVAIAPGGSTGIYFVTH--DAAGHHFHYRRAGSAASAYQAKA 114 Query: 123 FDADYFGAARHLHATGIAPALSPSTMAFAHHAMEVMRAAGKTISFDPNLRPMLWPSKEVM 182 + AA LH +GI+ A+S S AM RA+G T S D NLR LWP + Sbjct: 115 LPLEAIAAAGALHLSGISLAISESACEAGLAAMAHARASGVTTSLDTNLRLRLWPLERAR 174 Query: 183 AQQLNALAFKADWVLPGLSEGKILTGYEDPREIAGFYLERDVKMVVIKLGAEGAYWRTGE 242 A+ A D LP + +LTG ED I L VK+V KLG EG Y T + Sbjct: 175 ARMEEAFRL-CDICLPSWEDVSLLTGLEDRDAIVDRLLSYGVKLVAFKLGKEGCYVATSD 233 Query: 243 GEGRVAGVPVKEVVDTVGAGDGFAVGVISGMLEGLPVAQAVMRGNRIGAFAIQVVGDMEG 302 VA PV + +D GAGD F ++ ++ G A N A + G +E Sbjct: 234 QRRMVAPYPV-DSLDATGAGDCFGGAFMAELMAGRDAFAAARYANVAAALSTTGYGAVEP 292 Query: 303 LPTRAELDA 311 +P RA+++A Sbjct: 293 IPRRAQVEA 301 >gi|300311742|ref|YP_003775834.1| transferase kinase [Herbaspirillum seropedicae SmR1] Length = 657 Score = 94.7 bits (234), Expect = 9e-21, Method: Compositional matrix adjust. Identities = 88/326 (26%), Positives = 137/326 (42%), Gaps = 22/326 (6%) Query: 4 QLDVVTWGEALALLVADEVG-PFEEVEKYTRRLAGAETNVAIGLARLGLKVGWASRVGND 62 +LD++ G L A +VG P +V + + L G+ N+A G ARLGLK +R+G++ Sbjct: 20 RLDLICIGRLAVDLYAQQVGAPLSDVSSFAKYLGGSSANIAFGCARLGLKSAMLARIGDE 79 Query: 63 AFGRFIRKRVAQEGVDVSRVLTDMEFRTAIQLKAKAVDGADPAIEYYRKGSAASHLSLAD 122 A G+F++ +A EG DVS V D + TA+ + + P I +YR A L+ D Sbjct: 80 ANGQFLKAELAAEGCDVSHVTVDPQRLTALVMLGLKDKDSFPLI-FYRDNCADMALAPED 138 Query: 123 FDADYFGAARHLHATGIAPALSPSTMAFAHHAMEVMRAAGKTISFDPNLRPMLW------ 176 D + +++ L TG + +P + A++ R D + RP+LW Sbjct: 139 IDEAFIASSKALLITGTHFS-TPQVHRSSSLALQYARRHNVRTVLDIDYRPVLWGLTSKG 197 Query: 177 ------PSKEVMAQQLNALAFKADWVLPGLSEGKILTGYEDPREIAGFYLERDVKMVVIK 230 + + + Q L + + D V+ E I G D +V+K Sbjct: 198 DGETRFIASDGVTQHLQGILPQFDLVVGTEEEFMIAGGSTDIMAALRAVRALCAATLVVK 257 Query: 231 LGAEGAYWRTGEGEGRV------AGVPVKEVVDTVGAGDGFAVGVISGMLEGLPVAQAVM 284 G G G R+ GV V EV++ +GAGD F G + G L Sbjct: 258 RGPLGCVVLHGAIPERLDDGFHYRGVQV-EVLNVLGAGDAFISGFLKGWLNEEDDEHCCR 316 Query: 285 RGNRIGAFAIQVVGDMEGLPTRAELD 310 N GA + +P ELD Sbjct: 317 YANACGALVVSRHACAPAMPGPIELD 342 >gi|300310353|ref|YP_003774445.1| ribokinase [Herbaspirillum seropedicae SmR1] Length = 319 Score = 65.5 bits (158), Expect = 6e-12, Method: Compositional matrix adjust. Identities = 82/300 (27%), Positives = 114/300 (38%), Gaps = 68/300 (22%) Query: 37 GAETNVAIGLARLGLKVGWASRVGNDAFGRFIRKRVAQEGVDVSRVLTDMEFRTAIQLKA 96 G +N A+ AR+G V + S +G DAFG F + R I Sbjct: 45 GKGSNQAVAAARVGAPVRFISAIGKDAFGDFA---------------LSLWRRETIAPHV 89 Query: 97 KAVDGADP--AIEYYRKGS----------AASHLSLADFDADYFGAARHLHATGIAPAL- 143 + +DGA A Y S AAS LS D + R A A Sbjct: 90 RVIDGAPTGAAFIYVNDASGDNAIIVVPGAASQLSEQDVE-------RERQAIEAAKVFV 142 Query: 144 ----SPSTMAFAHHAMEVMRAAGKTISFDPNLRPMLWPSKEVMAQQLNALAFKA------ 193 P A A +++ RAAG T F+P ALAF A Sbjct: 143 TQLEQPPEAALA--GLQIARAAGTTTVFNPA----------------PALAFPAQVYGLC 184 Query: 194 DWVLPGLSEGKILTGYE-----DPREIAGFYLERDVKMVVIKLGAEGAYWRTGEGEGRVA 248 D++ P E +LTG E D R A LER V +I LGA+G+ + + Sbjct: 185 DFITPNEHEAALLTGIEIHHIDDARRAADVLLERGVGCALITLGAQGSLLHSRSQSLHLP 244 Query: 249 GVPVKEVVDTVGAGDGFAVGVISGMLEGLPVAQAVMRGNRIGAFAIQVVGDMEGLPTRAE 308 + VV+T GAGD F +G+ EG+ +A + A ++ G +P+R E Sbjct: 245 ALCAGTVVETAGAGDAFNGAFAAGLAEGMSAEEAARLATAVAAISVTRPGTAPSMPSRQE 304 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,060,454 Number of extensions: 48664 Number of successful extensions: 170 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 165 Number of HSP's successfully gapped: 6 Length of query: 314 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 229 Effective length of database: 1,217,395 Effective search space: 278783455 Effective search space used: 278783455 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)