BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1305.p (434 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310790|ref|YP_003774882.1| major facilitator superfamily p... 807 0.0 gi|300311827|ref|YP_003775919.1| sugar permease of the major fac... 684 0.0 gi|300309357|ref|YP_003773449.1| major facilitator superfamily (... 309 3e-85 gi|300312423|ref|YP_003776515.1| proline/betaine transporter pro... 303 3e-83 gi|300309575|ref|YP_003773667.1| proline/betaine transporter pro... 296 3e-81 >gi|300310790|ref|YP_003774882.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 432 Score = 807 bits (2085), Expect = 0.0, Method: Compositional matrix adjust. Identities = 413/427 (96%), Positives = 420/427 (98%), Gaps = 1/427 (0%) Query: 1 MTKTTQTAKRHSKLSTVLRVTSGNFLEMFDFFLYGFYASYISKTFFPSDNEFVSLILTFG 60 MT TT AK HSKLSTVLRVTSGNFLEMFDFFLYGFYASYISKTFFPSDNEFVSLILTFG Sbjct: 1 MTTTTTNAK-HSKLSTVLRVTSGNFLEMFDFFLYGFYASYISKTFFPSDNEFVSLILTFG 59 Query: 61 TFGAGFLMRPLGAIILGSYIDRVGRRQGLIVTLAIMALGTLLIAFVPGYATIGMAAPILV 120 TFGAGFLMRPLGAIILGSYIDRVGRRQGLIVTLAIMA+GTLLIAFVPGYATIG+AAPILV Sbjct: 60 TFGAGFLMRPLGAIILGSYIDRVGRRQGLIVTLAIMAIGTLLIAFVPGYATIGVAAPILV 119 Query: 121 LTGRLLQGFSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQAAIMASALIGYLVSQSL 180 LTGRLLQGFSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQAAIMASALIGYLVSQSL Sbjct: 120 LTGRLLQGFSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQAAIMASALIGYLVSQSL 179 Query: 181 SPLQISEWGWRIPFFIGCMIVPAIFIIRRSLQETEEFLARKRHPSFGEIMTSIGQNWKIV 240 SP+QIS+WGWRIPFFIGCMIVPAIFIIRRSLQET+EFLARKRHPSFGEIM+SI QNWKIV Sbjct: 180 SPVQISDWGWRIPFFIGCMIVPAIFIIRRSLQETDEFLARKRHPSFGEIMSSIAQNWKIV 239 Query: 241 VAGMMMVVMTTVSFYLITVYTPTFGKNVLKLSASDSLIVTFCVGLSNFCWLPIMGALSDR 300 VAGMMMVVMTTVSFYLITVYTPTFGKNVLKLSASDSLIVTFCVGLSNFCWLPIMGALSDR Sbjct: 240 VAGMMMVVMTTVSFYLITVYTPTFGKNVLKLSASDSLIVTFCVGLSNFCWLPIMGALSDR 299 Query: 301 VGRRPVLVAFTLLTLLTAYPVMSWLVSDISFKNLLITELWLSFLYASYNGAAVVALTEVM 360 VGRRPVLVAFTLLTLLTAYPVMSWLVSDISFKNLLITELWLSFLYASYNGAAVVALTEVM Sbjct: 300 VGRRPVLVAFTLLTLLTAYPVMSWLVSDISFKNLLITELWLSFLYASYNGAAVVALTEVM 359 Query: 361 PAHVRTVGFSLAYSLATAIFGGFTPLVSTWLIETSGDKAAPGYWMSFAAVCGLIATLVLY 420 PAHVRTVGFSLAYSLATAIFGGFTPLVSTWLIETSGDKAAPGYWMSFAAVCGL+ATLVLY Sbjct: 360 PAHVRTVGFSLAYSLATAIFGGFTPLVSTWLIETSGDKAAPGYWMSFAAVCGLLATLVLY 419 Query: 421 RGKGATA 427 RGK A A Sbjct: 420 RGKSAAA 426 >gi|300311827|ref|YP_003775919.1| sugar permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 684 bits (1766), Expect = 0.0, Method: Compositional matrix adjust. Identities = 336/426 (78%), Positives = 374/426 (87%), Gaps = 1/426 (0%) Query: 4 TTQTAKRHSKLSTVLRVTSGNFLEMFDFFLYGFYASYISKTFFPSDNEFVSLILTFGTFG 63 TT TA + SK STVLRVTSGNF+EMFDFFL+GFYA++ISK FFPSDNEF SL+LTF TFG Sbjct: 2 TTSTASQ-SKFSTVLRVTSGNFMEMFDFFLFGFYATHISKAFFPSDNEFASLMLTFMTFG 60 Query: 64 AGFLMRPLGAIILGSYIDRVGRRQGLIVTLAIMALGTLLIAFVPGYATIGMAAPILVLTG 123 AGFLMRPLGAIILG+Y+DRVGRRQGLIVTLA+MALGT+LIAFVP YATIG AP+LVL G Sbjct: 61 AGFLMRPLGAIILGAYVDRVGRRQGLIVTLALMALGTMLIAFVPSYATIGALAPLLVLIG 120 Query: 124 RLLQGFSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQAAIMASALIGYLVSQSLSPL 183 RLLQGFSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQ AI+ +A IGYL+S SL+P Sbjct: 121 RLLQGFSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQVAIIVAAAIGYLLSTSLTPA 180 Query: 184 QISEWGWRIPFFIGCMIVPAIFIIRRSLQETEEFLARKRHPSFGEIMTSIGQNWKIVVAG 243 ++S WGWRIPFFIGC+IVP +F+IRRSLQETEEF+ RK PS G+I S+ +NWK+V+AG Sbjct: 181 EVSGWGWRIPFFIGCLIVPVLFVIRRSLQETEEFMRRKHRPSTGQIFRSMIENWKLVIAG 240 Query: 244 MMMVVMTTVSFYLITVYTPTFGKNVLKLSASDSLIVTFCVGLSNFCWLPIMGALSDRVGR 303 MM+V MTTVSFYLITVYTPTFGKNVLKLS SLIVT CVGLSNF WLPIMGALSDRVGR Sbjct: 241 MMLVSMTTVSFYLITVYTPTFGKNVLKLSTEASLIVTLCVGLSNFIWLPIMGALSDRVGR 300 Query: 304 RPVLVAFTLLTLLTAYPVMSWLVSDISFKNLLITELWLSFLYASYNGAAVVALTEVMPAH 363 RP+LV FT+LT+LTAYP ++WLV D SF +LI ELWLSFLYASYNGA VVALTEVMPA Sbjct: 301 RPLLVTFTVLTILTAYPAVNWLVHDASFAKMLIVELWLSFLYASYNGAMVVALTEVMPAD 360 Query: 364 VRTVGFSLAYSLATAIFGGFTPLVSTWLIETSGDKAAPGYWMSFAAVCGLIATLVLYRGK 423 VRT GFSLAYSLATA+FGGFTP ++T LIE + DKAAPG WMS AAVCGLIATL+LYR K Sbjct: 361 VRTAGFSLAYSLATAVFGGFTPAIATGLIEYTHDKAAPGIWMSTAAVCGLIATLILYRNK 420 Query: 424 GATAAA 429 AA Sbjct: 421 AVRQAA 426 >gi|300309357|ref|YP_003773449.1| major facilitator superfamily (MFS) transporter protein [Herbaspirillum seropedicae SmR1] Length = 437 Score = 309 bits (791), Expect = 3e-85, Method: Compositional matrix adjust. Identities = 161/406 (39%), Positives = 238/406 (58%), Gaps = 2/406 (0%) Query: 12 SKLSTVLRVTSGNFLEMFDFFLYGFYASYISKTFFPSDNEFVSLILTFGTFGAGFLMRPL 71 +K V+ GN LE +DF +YGF+++ I++ FFP+DNE+ SL++ TFG GF MRP+ Sbjct: 6 NKQRQVIAAIVGNALEWYDFIVYGFFSAIIARLFFPADNEYTSLLVALATFGIGFFMRPV 65 Query: 72 GAIILGSYIDRVGRRQGLIVTLAIMALGTLLIAFVPGYATIGMAAPILVLTGRLLQGFSA 131 G ++LG Y DR GR+ + V + +M L +IAF P YATIG+AAPI+++ R+LQGF+ Sbjct: 66 GGVLLGLYADRKGRKAAMQVIIVLMTLAIAIIAFTPTYATIGIAAPIMIVVARMLQGFAT 125 Query: 132 GVELGGVSVYLAEMATPGNKGFYVSWQSASQQAAIMASALIGYLVSQSLSPLQISEWGWR 191 G E + +L E A +G Y SWQ Q A+ A +G LV+++LSP + WGWR Sbjct: 126 GGEYSSSTAFLVESAPAHKRGLYGSWQLVGQCLAVFCGAGMGALVTRNLSPEALDSWGWR 185 Query: 192 IPFFIGCMIVPAIFIIRRSLQETEEFLARKRHPSFGEI--MTSIGQNWKIVVAGMMMVVM 249 +PF +G +I P IRR + ET++FL P+ I + + N + V+ M V+ Sbjct: 186 LPFVLGLLIGPVGLWIRRHMHETDDFLESANKPNAAPIKLLDVVKTNLRGVLVSMGQVIN 245 Query: 250 TTVSFYLITVYTPTFGKNVLKLSASDSLIVTFCVGLSNFCWLPIMGALSDRVGRRPVLVA 309 TV+FY++ V PTF L L + +V +P GALSDR+GRR VL+ Sbjct: 246 GTVAFYVVLVNMPTFANKQLGLPLDQAFMVQMIAVALLTAVIPFAGALSDRLGRRTVLLY 305 Query: 310 FTLLTLLTAYPVMSWLVSDISFKNLLITELWLSFLYASYNGAAVVALTEVMPAHVRTVGF 369 +L LL YP+ W+ + S LL+ ++ L + + G AL+E P VR+ G Sbjct: 306 SSLAFLLMVYPLFVWVSAAPSLPRLLVMQVLLCIVIGANFGPMPTALSEQFPTRVRSTGL 365 Query: 370 SLAYSLATAIFGGFTPLVSTWLIETSGDKAAPGYWMSFAAVCGLIA 415 ++AY+LA +FGGF P + TWL +TSG AP +++ FAA G+ A Sbjct: 366 AVAYNLAAMLFGGFAPFIVTWLTKTSGSPVAPAWYVLFAACIGVSA 411 >gi|300312423|ref|YP_003776515.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 427 Score = 303 bits (775), Expect = 3e-83, Method: Compositional matrix adjust. Identities = 167/413 (40%), Positives = 247/413 (59%), Gaps = 7/413 (1%) Query: 5 TQTAKRHSKLSTVLRVTSGNFLEMFDFFLYGFYASYISKTFFPSDNEFVSLILTFGTFGA 64 T R + +S+ + GN LE FD +YG +A I+K FFP+ ++ VSL+LTF TFG Sbjct: 13 THPQARRAIISSSI----GNALEWFDILIYGAFAVVIAKQFFPTGDDSVSLLLTFATFGV 68 Query: 65 GFLMRPLGAIILGSYIDRVGRRQGLIVTLAIMALGTLLIAFVPGYATIGMAAPILVLTGR 124 F MRPLGA++LG+Y DR GR+ L++++ +M +GT +IAF+P YA+IG+ AP + G+ Sbjct: 69 SFFMRPLGAVVLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYASIGLLAPAGIALGK 128 Query: 125 LLQGFSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQAAIMASALIGYLVSQSLSPLQ 184 ++QGFSAG E G + +L E A P +GF+ SWQ ASQ +++ +AL G +++ L+P Q Sbjct: 129 MIQGFSAGGEFGSSTAFLVEHA-PHRRGFFSSWQVASQGISLLLAALFGAVLNNMLTPEQ 187 Query: 185 ISEWGWRIPFFIGCMIVPAIFIIRRSLQETEEFLARKRHPSFGEIMTSIGQNWKIVVAGM 244 ++ WGWR+PF G +I PA IRR L E EF + T Q ++++ G Sbjct: 188 LASWGWRVPFIFGLLIAPAGIYIRRHLDEAPEFKESSEKTNAPLRDTFAHQKMRLLI-GA 246 Query: 245 MMVVMTTVSFYLITVYTPTFGKNVLKLSASDSLIVTFCVGLSNFCWLPIMGALSDRVGRR 304 V+M TVS YL ++Y PT+ L L A S GL F P++GALSD++GR Sbjct: 247 GSVIMATVSVYL-SLYIPTYAVKQLGLPAWSSFAAMSVAGLIMFIGSPLVGALSDKIGRT 305 Query: 305 PVLVAFTLLTLLTAYPVMSWLVSDISFKNLLITELWLSFLYASYNGAAVVALTEVMPAHV 364 P ++ + L ++ YP+ +L + F LL+ + + L Y A L ++ P Sbjct: 306 PFMITSSALYIVLTYPMFVFLTNSPGFLQLLLLQTVIGVLMTMYFAAMPALLADIFPVAT 365 Query: 365 RTVGFSLAYSLATAIFGGFTPLVSTWLIETSGDKAAPGYWMSFAAVCGLIATL 417 R G SLAY++A IFGGF L+ TWLI+ +GDK + Y++ F AV LIA+L Sbjct: 366 RGTGMSLAYNIAVTIFGGFAGLIITWLIDFTGDKLSVSYFVIFGAVLSLIASL 418 >gi|300309575|ref|YP_003773667.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 296 bits (757), Expect = 3e-81, Method: Compositional matrix adjust. Identities = 165/417 (39%), Positives = 240/417 (57%), Gaps = 7/417 (1%) Query: 1 MTKTTQTAKRHSKLSTVLRVTSGNFLEMFDFFLYGFYASYISKTFFPSDNEFVSLILTFG 60 + + +R + ++TV+ GN LE FDF +Y F+A I+K FFP+ N+ SL+L Sbjct: 11 IVSVSNQQRRRAIVATVI----GNGLEWFDFTVYSFFAVIIAKLFFPTGNDLTSLLLAVA 66 Query: 61 TFGAGFLMRPLGAIILGSYIDRVGRRQGLIVTLAIMALGTLLIAFVPGYATIGMAAPILV 120 TFG GF MRP+G I+LG Y DRVGR+ L VT+ +MA+GT +I P Y +IG+ AP+++ Sbjct: 67 TFGVGFFMRPVGGILLGIYADRVGRKAALSVTILLMAVGTTMIGLAPTYESIGLFAPLII 126 Query: 121 LTGRLLQGFSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQAAIMASALIGYLVSQSL 180 + RLLQGFSAG E+G + +L E A + +Y SW AS A++ A +G V+ SL Sbjct: 127 VVARLLQGFSAGGEMGSATAFLTEYAPERERAYYSSWIQASIGVAVLLGAAVGTFVTASL 186 Query: 181 SPLQISEWGWRIPFFIGCMIVPAIFIIRRSLQETEEFLARKRHPSFGEIMTSIGQNW-KI 239 ++ WGWR+PF +G +I P + IR L+ET FL K + + +N+ + Sbjct: 187 DAAALASWGWRLPFLLGIIIGPVGYYIRHHLEETPTFLEEKDKTD--SPLKEVIRNFPRE 244 Query: 240 VVAGMMMVVMTTVSFYLITVYTPTFGKNVLKLSASDSLIVTFCVGLSNFCWLPIMGALSD 299 A MV++ TV Y++ Y PT+ VLKL + I GL PI+G L+D Sbjct: 245 TSASFSMVILWTVCTYVLLFYMPTYSVKVLKLPQTTGFIAGMVGGLCIMVLAPIVGRLAD 304 Query: 300 RVGRRPVLVAFTLLTLLTAYPVMSWLVSDISFKNLLITELWLSFLYASYNGAAVVALTEV 359 R+GRRP+L L L+ AYP+ +++ S S LLI +L L A+Y G + A +E+ Sbjct: 305 RIGRRPLLSGSAALILMLAYPMFAYINSAPSLSTLLIFQLVFGTLIAAYTGPILAAFSEL 364 Query: 360 MPAHVRTVGFSLAYSLATAIFGGFTPLVSTWLIETSGDKAAPGYWMSFAAVCGLIAT 416 PA V + G S+AY+LA IFGGF TWLI +G AP +++ AA + T Sbjct: 365 FPAKVLSTGLSVAYNLAVTIFGGFASFFITWLIAATGSTMAPAFYVMIAAAISFVGT 421 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.138 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,269,199 Number of extensions: 50841 Number of successful extensions: 326 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 297 Number of HSP's successfully gapped: 14 Length of query: 434 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 346 Effective length of database: 1,203,190 Effective search space: 416303740 Effective search space used: 416303740 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)