BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1301.p (349 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313279|ref|YP_003777371.1| vanillate O-demethylase oxygena... 109 4e-25 gi|300309801|ref|YP_003773893.1| phenylpropionate dioxygenase [H... 90 3e-19 >gi|300313279|ref|YP_003777371.1| vanillate O-demethylase oxygenase subunit oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 349 Score = 109 bits (272), Expect = 4e-25, Method: Compositional matrix adjust. Identities = 58/173 (33%), Positives = 93/173 (53%), Gaps = 3/173 (1%) Query: 21 KDLWYPICPSEFVAEKPVSLRRLGKKFVFWREPSGQLHALEDHCPHRGAPLSMGIPMGDR 80 K+ WY C + + KP+ + G+K VF+R G++ A+ED CPHRGAPLS+G + Sbjct: 4 KNAWYVACTPDEIDSKPLGRQICGEKIVFYRGAGGKVAAVEDFCPHRGAPLSLGFVREGQ 63 Query: 81 IACGYHGVQVRFDGVVTSVPGSPGCKLEGAKATRRFHVTEAAGAIFLYNSLEDIDTPPPL 140 + CGYHG+++ DG S+PG ++ G R + V E G I+++ ++ P + Sbjct: 64 LVCGYHGLEMGCDGKAISMPGQ---RVRGFPCIRSYPVEERYGFIWVWPGDKEQADPAQI 120 Query: 141 RLPEELTSPEYSHFLCYTEWKGDYRYVLDNVMDPMHGTFLHKQSHSMAEGDNT 193 E +PE+++ DYR ++DN+MD H T++H S E D Sbjct: 121 HHLEWADNPEWAYGGGLYHINCDYRLMIDNLMDLTHETYVHASSIGQKEIDEA 173 >gi|300309801|ref|YP_003773893.1| phenylpropionate dioxygenase [Herbaspirillum seropedicae SmR1] Length = 343 Score = 90.1 bits (222), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 83/307 (27%), Positives = 127/307 (41%), Gaps = 34/307 (11%) Query: 20 LKDLWYPICPSEFVAEKPVSLRRLGKKFVFWREPSGQLHALEDHCPHRGAPLSMGIPMGD 79 L+ WY + P + + P LG+K V W + G AL+D C HR A LS G Sbjct: 9 LRRFWYALMPIAQLRQGPQPFTLLGEKLVVWLKEDGAPAALQDRCCHRTARLSKGFVENG 68 Query: 80 RIACGYHGVQVRFDGVVTSVPGSPGCKLEGAKATRRFHVTEAAGAIFLYNSLEDIDTPPP 139 I CGYHG +G VP + + + + E G +++ +LE+ P Sbjct: 69 NIVCGYHGWTFNGEGACVRVPQNADGSVPAGACVKSYFCKERYG--YVWVALEE-----P 121 Query: 140 LR-LPE--ELTSPEYSH-FLCYTEWKGDYRYVLDNVMDPMHGTFLHKQSHSMAEGDNTAS 195 LR +PE E +P Y F Y W+ +++N D H +++H+ + + + Sbjct: 122 LRDIPEFPEEGAPGYRRIFQFYERWETSALRMMENSFDNSHFSYVHRATFGLYDQPKPEK 181 Query: 196 FGIRETDIGFVFE-KEGQRDVNFDWTEWG---DTGLHWM----------RLAIPYPKTGG 241 + I ETD GF E + ++V G DT + M R YP++ Sbjct: 182 YEIAETDYGFEAETRVPIKNVPASHRVTGSTADTTVRHMFNRWYLPFVRRFGCTYPES-- 239 Query: 242 PGGSFVIIGSYTPIVENIAAVFHWRCRKLTGWQRDTWRFLYRNRLEARHWNVLEQDRVVL 301 G +I TPI + + W R DT L A +L +DR +L Sbjct: 240 -GRHHIIYNCATPIDDGSIMLSQWLYRN------DTEEECSEAELIAWDAPILVEDREIL 292 Query: 302 ELMEPDA 308 E +PDA Sbjct: 293 EATDPDA 299 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.138 0.448 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,035,517 Number of extensions: 44057 Number of successful extensions: 102 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 99 Number of HSP's successfully gapped: 2 Length of query: 349 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 263 Effective length of database: 1,212,660 Effective search space: 318929580 Effective search space used: 318929580 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)