BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1299.p (386 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310665|ref|YP_003774757.1| monooxygenase FAD-binding prote... 283 1e-77 gi|300310340|ref|YP_003774432.1| FAD-dependent 2-polyprenyl-6-me... 114 1e-26 gi|300312379|ref|YP_003776471.1| FAD-dependent monooxygenase [He... 79 5e-16 gi|300311950|ref|YP_003776042.1| 2-polyprenyl-6-methoxyphenol hy... 69 5e-13 >gi|300310665|ref|YP_003774757.1| monooxygenase FAD-binding protein [Herbaspirillum seropedicae SmR1] Length = 384 Score = 283 bits (725), Expect = 1e-77, Method: Compositional matrix adjust. Identities = 142/352 (40%), Positives = 205/352 (58%), Gaps = 3/352 (0%) Query: 25 GHEVVVYEQSRQFLRVGADINLTPNAVRALDGLGIGEAVRRTAARPTHRISRTWNTGEET 84 G V VYEQ+ F R+GA I++ PN ++ L +GI + + R +RP SR W TG+ Sbjct: 27 GFNVRVYEQAPGFSRLGAGIHVGPNVMKVLRRIGIEDEMNRQGSRPDFWYSRHWQTGDVL 86 Query: 85 SRLAMGDTAEQKYGAPQLTIHRADLLAALAEVFPLEQVRFAKRAEQISEQGDGLHLHFAD 144 +++ +GD A + YGA LT+HR D L + P E++ F K ++ ++GD + L FAD Sbjct: 87 AQIPLGDYAVKHYGASYLTVHRGDFHELLIKALPSERLSFGKCLTKVEDRGDVVILSFAD 146 Query: 145 GSRERVDVLIGGDGIHSAVRTAMFGAERPRFTGVVAFRAVVPAAKV-ASLPNLQAFTKWW 203 G+ E D++IG DG++S +R + G E P++ G +A RAV P ++ A + A KWW Sbjct: 147 GTTEEADIVIGADGVNSRIREELLGVEPPKYAGYLAHRAVFPTPELKAGMLPFDACVKWW 206 Query: 204 GPNPQSQIVTFPLNRGQDIFIFATTAQESWHLESWTTPGNVEELRESYAGYHPDATALLD 263 + + +V F ++ +++ E W L P + EE+RE++ G+HP AL+D Sbjct: 207 SDD-RHMMVYFVTSKANELYYVTGVPVEKWDLNDRWLPSSKEEMRETFHGWHPTVQALVD 265 Query: 264 ACDEVLKTALYERDPLPAWARGNMALLGDACHPMMPFMAQGAGMAIEDAVVLARCLARAT 323 A EV K +L ERDPLP W+RG + LLGDACHPM P MAQGA MAIED +LARC + Sbjct: 266 ATVEVTKWSLLERDPLPLWSRGRLVLLGDACHPMKPHMAQGAAMAIEDGAMLARCF-KEV 324 Query: 324 ALEQVAACLHTYEQLRMERASTIQIGSRGNNWLRAGGNADWVYGYDAWSTPL 375 + A YE R RAS +Q S N WLR + W +GYD ++ PL Sbjct: 325 GVSNYADAFALYEANRAARASKVQRISHDNTWLRTNEDPSWCFGYDVFTEPL 376 >gi|300310340|ref|YP_003774432.1| FAD-dependent 2-polyprenyl-6-methoxyphenol hydroxylase [Herbaspirillum seropedicae SmR1] Length = 376 Score = 114 bits (285), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 104/373 (27%), Positives = 173/373 (46%), Gaps = 33/373 (8%) Query: 4 KIAILGAGIGGLTAAIALQRAGHEVVVYEQSRQFLRVGADINLTPNAVRALDGLGIGEAV 63 +I I+G G G++AAI L+R G +V + E Q+ GA I+L P +RAL LG+ EA Sbjct: 7 RILIIGGGFSGMSAAIDLRRRGAQVDLVELDAQWRNYGAGISLGPATLRALKQLGVLEAF 66 Query: 64 RRTAARPTHRISRTWNTGEETSRLAMGDTAEQKYGAPQLTIHRADLLAALAEVFPL---- 119 R A + G + + L + +P + A L LA + Sbjct: 67 LREGA-AGDGVRLCLPHGPQVAELPT-----PRLASPDVPGGGAILRPVLARILADATRA 120 Query: 120 --EQVRFAKRAEQISEQGDGLHLHFADGSRERVDVLIGGDGIHSAVRTAMFG-AERPRFT 176 VR + E G + + F DG R D++IG DG++S +R +F A +PR++ Sbjct: 121 AGADVRLGCTFSAVREVGHKVEVDFTDGQTRRYDLVIGADGLYSKLRMHLFPHAPKPRYS 180 Query: 177 GVVAFRAVVPAAKVASLPNLQAFTKWWGPNPQSQIVTFPLNRGQDIFIFATTAQE-SWHL 235 G +RAV+P + + T W GP + + P+++ ++++F T + + H+ Sbjct: 181 GQAVWRAVLPRPQ-----EIVTCTMWMGPRIKPGV--NPVSK-DEMYLFVTEPRPVNEHV 232 Query: 236 ESWTTPGNVEELRESYAGYHPDATALLDACDEVLKTALYERD----PLPAWARGNMALLG 291 + T ++ L E ++ P + + D+ + + P P W +G + L+G Sbjct: 233 DPTTFVSHLRGLLEEFSA--PVLKTIREQLDDNARIIFRPLEGLLLPRP-WYQGRVVLIG 289 Query: 292 DACHPMMPFMAQGAGMAIEDAVVLARCLARATALEQVAACLHTYEQLRMERAS-TIQIGS 350 DA H P +A GA + IEDA+VLA L R + Q L +E+ R ER ++ + Sbjct: 290 DAVHATTPHLASGACIGIEDALVLADELERHGTVPQ---ALAAFEERRWERCRMVVENSA 346 Query: 351 RGNNWLRAGGNAD 363 R GG+ D Sbjct: 347 RLGEIEVEGGDKD 359 >gi|300312379|ref|YP_003776471.1| FAD-dependent monooxygenase [Herbaspirillum seropedicae SmR1] Length = 417 Score = 79.3 bits (194), Expect = 5e-16, Method: Compositional matrix adjust. Identities = 102/363 (28%), Positives = 145/363 (39%), Gaps = 37/363 (10%) Query: 3 FKIAILGAGIGGLTAAIALQRAGHEVVVYEQSRQFLRVGADINLTPNAVRALDGLGIGEA 62 +AI GAG GL A+ L+RAGH V + E+ Q VG+ + L P + L LG+ Sbjct: 4 LHVAIAGAGPAGLAMALYLKRAGHRVSLLERFLQAAPVGSGLILQPTGLTVLQDLGL--- 60 Query: 63 VRRTAARPTHRISRTWN----TGEETSRLAMGDTAEQKYGAPQLTIHRADLLAALAEVFP 118 R AA RI R + +G+ + A +YG + +HRA L L E Sbjct: 61 FSRIAAL-GQRIDRLYGADALSGKTVLDVRYDAIAGGRYG---IAVHRAALFGVLLEAAQ 116 Query: 119 LE--QVRFAKRAEQISEQGDGLHLHFADGSR-ERVDVLIGGDGIHSAVRTAMFGAERPRF 175 E +V + + G L ADG R D++I G S +R + PR Sbjct: 117 QEGIEVETGQDIGNLEHTGAQACLTAADGRRIGGFDLVIDASGARSPLRRHALHSCEPRP 176 Query: 176 TGVVAFRAVVPAAKVASLPNLQAFTKWWGPNPQSQIVTFPLNRGQDIFIFATTAQESWHL 235 AF + A S + ++ + I P+ R T E Sbjct: 177 LPYGAFWVSLRAQPGLSFGHNSLVQRYR--RASTMIGVLPIGR-------PTPQDEPMVA 227 Query: 236 ESWTT-PGNVEELRES-----YAGYH---PDATALLDACD--EVLKTALYERDPLPAWAR 284 W+T P VE L+++ G H P+ L D E + A Y LP Sbjct: 228 FFWSTRPDRVESLKQAGLSAWKDGVHALWPECAPYLAQIDGFEQMTLARYGHHTLPLPYG 287 Query: 285 GNMALLGDACHPMMPFMAQGAGMAIEDAVVLARCLARATALEQVAACLHTYEQLRMERAS 344 +A++GDA H P + QGA MA+ DA L+ L ++ LEQ L Y R Sbjct: 288 ERLAIIGDAAHSTSPQLGQGANMALLDARALSHALQQSETLEQ---ALPRYAASRRRHVR 344 Query: 345 TIQ 347 T Q Sbjct: 345 TFQ 347 >gi|300311950|ref|YP_003776042.1| 2-polyprenyl-6-methoxyphenol hydroxylase [Herbaspirillum seropedicae SmR1] Length = 407 Score = 69.3 bits (168), Expect = 5e-13, Method: Compositional matrix adjust. Identities = 86/318 (27%), Positives = 133/318 (41%), Gaps = 34/318 (10%) Query: 21 LQRAGHEVVVYEQSRQFLRVGADINLT-PN--AVRALDGLG-IGEAVRRTAARPTHRISR 76 +QRAG V+V EQ+ + G + L+ P VR +D + +GE + +R R Sbjct: 29 MQRAGWRVLVVEQAEELRDGGHMMGLSGPGLATVRRMDLVARLGERAYPDMGQHIYRDRR 88 Query: 77 TWNTGEETSRLAMGDTAEQKYGAPQLTIHRADLLAALAEVFPLEQV--RFAKRAEQISEQ 134 G E R+ + E G LT+ R L+ L P + RF ++ Q Sbjct: 89 ----GREILRI---NYREALAGIEWLTLRRGVLVQELRAALPTRDIAWRFGVTVQEALNQ 141 Query: 135 GDGLHLHFADGSRERVDVLIGGDGIHSAVRTAMFGAERPRFTGVVAFRAVVPAAKVASLP 194 + + +DGS D+LI DG+HS++R F A+ + +R + L Sbjct: 142 ASSVRVSLSDGSCHEADLLIAADGVHSSLRRRHFAAD-ANCLHPLGYRYASYEVQDHLLL 200 Query: 195 NLQAFTKWWGPNPQSQIVTFPLNRGQDIFI-------FATTAQESWHLESWTTPGNVEEL 247 Q F + P Q++ R + + F AQ L + T Sbjct: 201 G-QDFLSYAEPGIQTEYYGLCDGRLAALHVWRSREAGFVPPAQRHSLLRTCTA------- 252 Query: 248 RESYAGYHPDATALLDACDEVL-KTALYERDPLPAWARGNMALLGDACHPMMPFMAQGAG 306 S+ G A+ + + V+ AL E LP+W +G + LLGDA H + QGAG Sbjct: 253 -RSFTGVRATLEAVENPQEIVIDDLALVE---LPSWRQGRILLLGDAAHSLSLISGQGAG 308 Query: 307 MAIEDAVVLARCLARATA 324 +A+ A +LA L RA A Sbjct: 309 VALASASILAEELERAAA 326 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.133 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,261,275 Number of extensions: 54168 Number of successful extensions: 193 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 186 Number of HSP's successfully gapped: 4 Length of query: 386 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 299 Effective length of database: 1,207,925 Effective search space: 361169575 Effective search space used: 361169575 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)