BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1297.p (492 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde deh... 325 4e-90 gi|300310293|ref|YP_003774385.1| NAD-dependent aldehyde dehydrog... 325 7e-90 gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrog... 301 8e-83 gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 300 2e-82 gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde ... 296 2e-81 >gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 325 bits (834), Expect = 4e-90, Method: Compositional matrix adjust. Identities = 180/469 (38%), Positives = 267/469 (56%), Gaps = 15/469 (3%) Query: 20 SGEPYATHYPATGEVVATLHAASVADVEEAIAGADLAFRSSGWAHKKAHERAAVLYRVAQ 79 GE + P G + +S DVE A+ A A +S W ERA +L ++A Sbjct: 32 KGEYFENISPVIGRAFCEVARSSAEDVELALDAAHAAKKS--WGKTSPTERANMLLKIAD 89 Query: 80 LIRERSEALAQMQRLDNGKPISETRAL-VASAAATFQFFAAACETLEETLTPMRGDNLTM 138 + E LA + LDNGKPI ET A + A F++FAAA T E ++ P+ D Sbjct: 90 RMEANLELLATAETLDNGKPIRETMAADIPLAIDHFRYFAAAVRTQEGSICPIDNDTYAY 149 Query: 139 SLYEPMGVVAAITPWNSPIASEAQKMAPALAAGNAVVVKPAEVTPLAALELARICIEAGV 198 +EP+GVV I PWN PI K+APALAAGN VV+KPAE TP + + L + + + Sbjct: 150 HFHEPLGVVGQIIPWNFPILMAVWKLAPALAAGNCVVLKPAEQTPASIMVLIELIADL-I 208 Query: 199 PAGMISVLPGKGSVIGDAITRHPLVKRVSFTGGTTTGKHIAHIAADKMMPVSLELGGKSP 258 P G+++++ G G G + + + +++FTG TTTG+ I A+ ++PV+LELGGKSP Sbjct: 209 PPGVVNIVQGFGVEAGKPLASNKRIAKIAFTGETTTGRLIMQYASQNLIPVTLELGGKSP 268 Query: 259 TMVFEDA------DVDHAVAGVLYGIFS-SSGESCIAGSRLFVARTLYDGFMERLAAAAA 311 + F D D A+ G + +F+ + GE C SR+ V ++Y+ F+ER A Sbjct: 269 NIFFADVLDKDDDFFDKALEG--FAMFALNQGEVCTCPSRVLVQESIYERFIERALKRVA 326 Query: 312 ALRVGDPADARTQMGPLITARHRQGIERYVELGVAEGGRIRTGGVRPQ-GAQFERGYYYT 370 A++ G+P D T +G + + I Y+++G EG ++ GG R + G GYY Sbjct: 327 AIKQGNPLDKSTMIGAQASQEQLEKILSYIDIGKQEGAKVLAGGGREELGGDLASGYYVK 386 Query: 371 PTIIEGVSNQQRICQEEIFGPVLVAMPFDDEDDLIAQANDSVYALAAGIWTRDYKRAWRI 430 PT+ +G +N+ RI QEEIFGPV+ F DE++ +A AND++Y L AG+WTRD RA+R+ Sbjct: 387 PTVFQG-NNKMRIFQEEIFGPVVSVTTFKDEEEALAIANDTLYGLGAGLWTRDGTRAFRM 445 Query: 431 GRAVQAGNVWINTYKQFSISTPFGGWRDSGLGREKGRLGILQYMEQKAM 479 GR +QAG VW N Y + FGG++ SG+GRE ++ + Y + K + Sbjct: 446 GREIQAGRVWTNCYHLYPAHAAFGGYKQSGIGRENHKMMLDHYQQTKNL 494 >gi|300310293|ref|YP_003774385.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 504 Score = 325 bits (832), Expect = 7e-90, Method: Compositional matrix adjust. Identities = 180/469 (38%), Positives = 266/469 (56%), Gaps = 6/469 (1%) Query: 12 IAGQWRLGSGEPYATHYPATGEVVATLHAASVADVEEAIAGADLAFRSSGWAHKKAHERA 71 I G+ G GE + PATG A+L A W R Sbjct: 27 INGELVAGQGEIIQLYNPATGR--ASLSYRDGGAAAVEAAAVAAQRAQRQWWALSHAARG 84 Query: 72 AVLYRVAQLIRERSEALAQMQRLDNGKPISETRALVASAAATFQFFAAACETLEETLTPM 131 LY V +IR +E LA+++ + +GKPI + RA + A F+++A + + P+ Sbjct: 85 RALYAVGAVIRAEAEPLARLEAISSGKPIRDCRAEMQKVAEMFEYYAGWADKFYGEVIPV 144 Query: 132 RGDNLTMSLYEPMGVVAAITPWNSPIASEAQKMAPALAAGNAVVVKPAEVTPLAALELAR 191 +L + EP G V +TPWN+P + ++ PALA GNAV++KP+E+TP ++L +AR Sbjct: 145 PSSHLNYTRREPYGTVLQMTPWNAPAFTCGWQLGPALATGNAVLLKPSELTPFSSLAIAR 204 Query: 192 ICIEAGVPAGMISVLPGKGSVIGDAITRHPLVKRVSFTGGTTTGKHIAHIAADKMMPVSL 251 + +AG+PAG+++VL G G + VK+V F G TG IA AA ++MP L Sbjct: 205 LGEQAGLPAGLVNVLAGLGQTMVPQAMATWTVKKVIFVGSPATGALIAKAAAARVMPCVL 264 Query: 252 ELGGKSPTMVFEDADVDHAVAGVLYGIFSSSGESCIAGSRLFVARTLYDGFMERLAAAAA 311 ELGGKS ++FEDAD+ A G IFS +G+SC+AGSRL V R +YD F+E +AA A Sbjct: 265 ELGGKSANIIFEDADLRLAAFGAQAAIFSGAGQSCVAGSRLLVQRKVYDRFVETVAAGAE 324 Query: 312 ALRVGDPADARTQMGPLITARHRQGIERYVELGVAEGGRIRTGGVRPQGAQFERGYYYTP 371 +R+G P D T++GP+ + + I+R V G+ G + G R E GY+ P Sbjct: 325 KIRLGAPLDDSTEVGPINNRKQYEHIQRMVARGLEAGATLAAGHTR----YGEEGYFVRP 380 Query: 372 TIIEGVSNQQRICQEEIFGPVLVAMPFDDEDDLIAQANDSVYALAAGIWTRDYKRAWRIG 431 T++ SN + + EIFGPV VA+PF+DE++ IA ANDS + LA +WTRD RA R+ Sbjct: 381 TLLAHASNAMEVARSEIFGPVAVAIPFEDEEEAIAIANDSEFGLAGAVWTRDVARAHRVA 440 Query: 432 RAVQAGNVWINTYKQFSISTPFGGWRDSGLGREKGRLGILQYMEQKAMY 480 +V AG W+N+YK ++++PFGG+ SG GR G + Y + K+++ Sbjct: 441 ASVNAGTFWVNSYKTINVASPFGGFNRSGYGRSSGMEALYDYTQTKSVW 489 >gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 301 bits (771), Expect = 8e-83, Method: Compositional matrix adjust. Identities = 174/463 (37%), Positives = 258/463 (55%), Gaps = 17/463 (3%) Query: 12 IAGQWRL-GSGEPYATHYPATGEVVATLHAASVADVEEAIAGADLAFRSSGWAHKKAHER 70 IA +W SGE P+ G+ + + D+++A+ A LA+ + W A ER Sbjct: 6 IANRWVAPTSGESIPVLDPSDGQPFDAIARGNATDIDQAVRAARLAYEGA-WGRLSAAER 64 Query: 71 AAVLYRVAQLIRERSEALAQMQRLDNGKPISETRALVASAAATFQFFAAACETLEETLTP 130 + +L ++A +++ E LAQ++ D GKP+ + RA A+ F+F+A A + L P Sbjct: 65 SRLLMKLAYKLQDHQEELAQLEGRDCGKPMKQARADAAAIVRYFEFYAGAADKLHGDTIP 124 Query: 131 MRGDNLTMSLYEPMGVVAAITPWNSPIASEAQKMAPALAAGNAVVVKPAEVTPLAALELA 190 + ++L EP GV I PWN P+ + + ALAAGN VVKPAE L+ L +A Sbjct: 125 YQAGYTVLTLREPHGVTGHIVPWNYPMQIFGRCVGGALAAGNTCVVKPAEDACLSILRVA 184 Query: 191 RICIEAGVPAGMISVLPGKGSVIGDAITRHPLVKRVSFTGGTTTGKHIAHIAADKMMPVS 250 + E G P G ++++ G G GD + +HP V +SFTG GK + AA+ PV+ Sbjct: 185 ELAAEVGFPEGALNIVTGYGHEAGDLLVKHPDVNHISFTGSPNVGKLVVRAAAEHHTPVT 244 Query: 251 LELGGKSPTMVFEDADVDHAVAGVLYGIFSSSGESCIAGSRLFVARTLYDGFMERLAAAA 310 LELGGKSP +VFEDAD++ + V+ I ++G++C AGSRL V R Y+ +ERL+ Sbjct: 245 LELGGKSPQIVFEDADMEAMLPVVVNAIVQNAGQTCSAGSRLLVQRGAYEQVLERLSGLF 304 Query: 311 AALRVGDPADARTQMGPLITARHRQGIERYVE------LGVAEGGRIRTGGVRPQGAQFE 364 + L+VG + GPLI ++ + ++ + +A G+I G P G Sbjct: 305 SQLQVGS-SQMDLDCGPLIRKTQQERVAGFLSDAQRDGIALAAQGKIAAG--VPTG---- 357 Query: 365 RGYYYTPTIIEGVSNQQRICQEEIFGPVLVAMPFDDEDDLIAQANDSVYALAAGIWTRDY 424 G+Y P ++ V + R+ QEE+FGPVL AMPF+DE IA AN + Y L A +WTRD Sbjct: 358 -GFYQAPALLRDVPPRHRLAQEEVFGPVLAAMPFEDEAHAIALANGTDYGLVASVWTRDG 416 Query: 425 KRAWRIGRAVQAGNVWINTYKQ-FSISTPFGGWRDSGLGREKG 466 R R+ RA+++G V+IN Y + PFGG + SG GREKG Sbjct: 417 GRQMRLARAIRSGQVFINNYGAGGGVELPFGGVKASGHGREKG 459 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 300 bits (768), Expect = 2e-82, Method: Compositional matrix adjust. Identities = 171/473 (36%), Positives = 268/473 (56%), Gaps = 8/473 (1%) Query: 12 IAGQW-RLGSGEPYATHYPATGEVVATLHAASVADVEEAIAGADLAFRSSGWAHKKAHER 70 + GQW GE A H PA GE++ + A+ AI A+ A+ + W K A ER Sbjct: 16 VNGQWCDADQGERLAVHNPANGELLGHVPLMGAAETRRAIEAANAAWPA--WKKKTAKER 73 Query: 71 AAVLYRVAQLIRERSEALAQMQRLDNGKPISETRALVASAAATFQFFAAACE-TLEETLT 129 +A+L R +L+ ++ LA + + GKP++E R + AA+ ++FA + T +T+ Sbjct: 74 SAILRRWYELMLANTDDLALIMTAEQGKPLAEARGEIGYAASFIEWFAEEGKRTYGDTIP 133 Query: 130 PMRGDNLTMSLYEPMGVVAAITPWNSPIASEAQKMAPALAAGNAVVVKPAEVTPLAALEL 189 N + + E +GV AAITPWN P A +K PALAAG +V+KPAE TP +AL L Sbjct: 134 SPSPSNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVLKPAEATPFSALAL 193 Query: 190 ARICIEAGVPAGMISVLPGKGSVIGDAITRHPLVKRVSFTGGTTTGKHIAHIAADKMMPV 249 A + AG+PAG+ SV+ G IG +T +P+V+++SFTG T GK + +A + + Sbjct: 194 AVLAERAGIPAGVFSVVTGTPKGIGGEMTSNPIVRKISFTGSTGVGKLLMQQSASSIKKL 253 Query: 250 SLELGGKSPTMVFEDADVDHAVAGVLYGIFSSSGESCIAGSRLFVARTLYDGFMERLAAA 309 SLELGG +P +VF+DAD+D AV G + + ++G++C+ +R++V +YD F +L A Sbjct: 254 SLELGGNAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRIYVQDGVYDAFAAKLVEA 313 Query: 310 AAALRVGDPADARTQMGPLITARHRQGIERYVELGVAEGGRIRTGGVRPQGAQFERGYYY 369 +VG + GPLI + Q +E++V VA+G R+ GG R ++ Sbjct: 314 VKKFKVGQGTEEGVTQGPLINEQAVQKVEQHVADAVAKGARVLLGGKRHALGH----SFF 369 Query: 370 TPTIIEGVSNQQRICQEEIFGPVLVAMPFDDEDDLIAQANDSVYALAAGIWTRDYKRAWR 429 PT++ V+ ++ +EE FGP+ F +++++A AND+ + LA+ ++RD R WR Sbjct: 370 EPTVLADVTPAMQVAREETFGPMAPLFRFKTDEEVLALANDTEFGLASYFYSRDIGRIWR 429 Query: 430 IGRAVQAGNVWINTYKQFSISTPFGGWRDSGLGREKGRLGILQYMEQKAMYWG 482 + +++G V INT + PFGG + SGLGRE GI Y+ K + G Sbjct: 430 VAEGLESGMVGINTGLISNEVAPFGGVKQSGLGREGSHYGIDDYLVVKYLCMG 482 >gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 486 Score = 296 bits (759), Expect = 2e-81, Method: Compositional matrix adjust. Identities = 191/470 (40%), Positives = 260/470 (55%), Gaps = 10/470 (2%) Query: 12 IAGQWRLGSG-EPYATHYPATGEVVATLHAASVADVEEAIAGADLAFRSSGWAHKKAHER 70 I G+W+ G E A PAT E V + + ADV++A+ A A WA ER Sbjct: 20 IDGKWQPPQGSERAAVVAPATEERVCEIALGNAADVDQAVQAARRAI--PAWAATSPLER 77 Query: 71 AAVLYRVAQLIRERSEALAQMQRLDNGKPISETRALVASAAATFQFFAAACETLEETLTP 130 AA+L RV L+ ERSE AQ L+ G PIS R AA + A + L + Sbjct: 78 AALLGRVHALLLERSELFAQALTLEMGAPISYARTAHVPLAA--EHLRVARDNLADYPF- 134 Query: 131 MRGDNLTMSLYEPMGVVAAITPWNSPIASEAQKMAPALAAGNAVVVKPAEVTPLAALELA 190 +R T + EP+GV A ITPWN PI K+ PALAAG VV+KP+E++PL+AL A Sbjct: 135 IRPRGTTAIVREPIGVCALITPWNWPIYQITAKVGPALAAGCTVVLKPSELSPLSALLFA 194 Query: 191 RICIEAGVPAGMISVLPGKGSVIGDAITRHPLVKRVSFTGGTTTGKHIAHIAADKMMPVS 250 I EAGVPAG+ +++ G G+ +G A++ HP V +S TG T G +A AA + V+ Sbjct: 195 EIVHEAGVPAGVFNLVNGSGAEVGAALSAHPQVDMISITGSTRAGVLVAQAAAVTVKRVA 254 Query: 251 LELGGKSPTMVFEDADVDHAVAGVLYGIFSSSGESCIAGSRLFVARTLYDGFMERLAAAA 310 ELGGKSP +V DAD++ A+ + + G+SC A +RL V R E A Sbjct: 255 QELGGKSPNLVLPDADLERAIPPGVAAALRNMGQSCSAPTRLIVPRAALARVHELALATL 314 Query: 311 AALRVGDPADARTQMGPLITARHRQGIERYVELGVAEGGRIRTGGV-RPQGAQFERGYYY 369 A ++VGDP DA T GPL +++ +E G+ +G R+ GG RP G +GYY Sbjct: 315 AQMKVGDPNDAATTHGPLANRAQFLRVQQMIEAGLQDGARLLAGGPGRPDG--LAQGYYA 372 Query: 370 TPTIIEGVSNQQRICQEEIFGPVLVAMPFDDEDDLIAQANDSVYALAAGIWTRDYKRAWR 429 PTI V I QEEIFGPVL +P+D ++ IA AND+VY L A + RD + Sbjct: 373 RPTIFSEVHTDMVIAQEEIFGPVLAILPYDTVEEAIAIANDTVYGLGAHVQGRDKDQVRA 432 Query: 430 IGRAVQAGNVWINTYKQFSISTPFGGWRDSGLGREKGRLGILQYMEQKAM 479 + +Q+G V +N Y + PFGG++ SG GRE G G+ +YME KA+ Sbjct: 433 VAARIQSGQVHLN-YPAWDPQAPFGGYKQSGNGREYGVEGMEEYMEVKAI 481 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,641,788 Number of extensions: 73572 Number of successful extensions: 327 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 261 Number of HSP's successfully gapped: 20 Length of query: 492 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 403 Effective length of database: 1,198,455 Effective search space: 482977365 Effective search space used: 482977365 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)