BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1295.p (571 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311064|ref|YP_003775156.1| acetolactate synthase isozyme I... 122 6e-29 gi|300311584|ref|YP_003775676.1| pyruvate dehydrogenase [Herbasp... 84 4e-17 gi|300311743|ref|YP_003775835.1| acetolactate synthase [Herbaspi... 80 5e-16 >gi|300311064|ref|YP_003775156.1| acetolactate synthase isozyme III large subunit [Herbaspirillum seropedicae SmR1] Length = 572 Score = 122 bits (307), Expect = 6e-29, Method: Compositional matrix adjust. Identities = 129/543 (23%), Positives = 232/543 (42%), Gaps = 34/543 (6%) Query: 15 QVTVGCAIAAFLEQCGVKAAFGVISIHNMPILDAFGERGKIRFIPARGEAGGANMADAYA 74 Q+T + L + GV+ FG + I DA ++ K + I R E + ADAY+ Sbjct: 5 QLTGADILVRCLAEEGVEHVFGYPGGAVLYIYDAIFKQDKFQHILVRHEQAAVHAADAYS 64 Query: 75 RTTGGLGVCLTSTGTAAGNAAGAMVEALTAGTPLLHLTGQIETPYLDQSLAYIHEAPDQL 134 R++ +GV + ++G NA + A P++ ++GQ+ + + Q + D + Sbjct: 65 RSSQKVGVAIVTSGPGVTNAVTGLATAYMDSIPMVVISGQVPSTAIGQDA---FQECDTV 121 Query: 135 SMLKAISKAAFRIRSVETAISTIKLAVQTALTPPMGPVSVEIPIDI--QAAMISMPTNLQ 192 + + K F ++ V+ T+K A A T GPV V+IP DI + P ++ Sbjct: 122 GITRPCVKHNFLVKDVKDLAETVKKAFYIATTGRPGPVLVDIPKDITMHTCAYAYPKAVE 181 Query: 193 PLAIPP-SLPSARALDELAERLLKARRPLLWLGGGA--RHAGPQVQRLL-ALGFGVVTST 248 + P + + + + LL A RP+++ GGG +A P++ +L+ LG+ + Sbjct: 182 MRSYKPVDKGHSGQIRKALQLLLSAERPMIYTGGGIILANAAPELNKLVDRLGYPCTNTL 241 Query: 249 QGRGIVPEDDARSLGAFNLHKPVE-NF-YQSCDAMLVVGSRLR----GNETLKYELKLPR 302 G G + +G +H E N Q CD ++ +G+R GN K+ R Sbjct: 242 MGLGAYRSSSDKFVGMPGMHGTYEANMAMQHCDVLIAIGARFDDRVIGNP--KHFASHAR 299 Query: 303 PLYRVDADPAAEGRCYTSDYFVCGDSALVLGGLADRLAAL---QVDPAFMADLKQARESA 359 + +D DP++ + D + G+ VL L +L A Q A A KQ E Sbjct: 300 KIIHIDIDPSSISKRVKVDIPIVGNVKDVLQELLSQLDAAETRQNGAALDAWWKQINEWR 359 Query: 360 VHGLL------DGLGPYGTLVEQLQQAAGRQFNWVRDVTVSNSTWGNRYLRIFDPRAGVH 413 L + + P + + + G F + + W +Y PR ++ Sbjct: 360 KRDCLKFATSEEFIKPQSVVQKVWEVTKGDAF--ITSDVGQHQMWAAQYYGFDKPRRWIN 417 Query: 414 ALGGGIGQGLAMGIGAAIGAAVTQSGKKTFTLAGDGGFILNLGELATAVQEQADMVIVLM 473 + GG+G + +G+ A+G + + G+ + + ELAT Q I+L+ Sbjct: 418 S--GGLGT-MGVGLPYAMGVQMANPDATVACITGEASIQMCIQELATCKQYHLTPKIILL 474 Query: 474 NDQGYGVIKNIQDAQYGGR--RHYVDLHTPDYAALSAALKLRHKRVGDLSQVAAGLDEAL 531 N++ G+++ Q YG R Y+D PD+ L+ + ++ V L EA Sbjct: 475 NNRFLGMVRQWQQIDYGSRYSESYMD-SLPDFNKLAESYGHVGMKIEKPGDVDGALREAF 533 Query: 532 SQQ 534 + + Sbjct: 534 AMK 536 >gi|300311584|ref|YP_003775676.1| pyruvate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 564 Score = 83.6 bits (205), Expect = 4e-17, Method: Compositional matrix adjust. Identities = 125/539 (23%), Positives = 223/539 (41%), Gaps = 28/539 (5%) Query: 17 TVGCAIAAFLEQCGVKAAFGVISIHNMPILDAFGERGKIRFIPARGEAGGANMADAYART 76 TV + L + GV+ FGV+ P+ DA +I +I R E G A A A+ Sbjct: 4 TVADILIDTLYEIGVRQIFGVVGDALNPLTDAIRRDKRIEWIGVRHEEGAALAAAGQAKL 63 Query: 77 TGGLGVCLTSTGTAAGNAAGAMVEALTAGTPLLHLTGQIETPYLDQSLAYIHEAPDQLSM 136 TG L VC +TG A + + EA P+L ++G + P + Y+ E +L + Sbjct: 64 TGRLAVCCGTTGPGANHLVAGLYEARKDHAPVLAISGGV--PAAHRGTDYLQENSPEL-L 120 Query: 137 LKAISKAAFRIRSVETAISTIKLAVQTALTPPMGPVSVEIPIDIQAAMI--SMPTNLQPL 194 + ++ + I S + A + A+ A G + IP D+ A S+P+ Sbjct: 121 FRDVAAYSQIITSPDQAPAVFHQAIAQAYA-QRGVAHLNIPADVIGAKTSGSVPSLHTLR 179 Query: 195 AIPPSLPSARALDELAERLLKARRPLLWLGGGARHAGPQVQRLLA-LGFGVVTSTQGRGI 253 P P A + A+ + A ++ G G R A +V L + L V+ + +G+ + Sbjct: 180 ERTPLSPPAADIQAAADLINAAGSVAIFAGNGCRGAIDEVLALASKLQAPVMHTFRGKDL 239 Query: 254 VPEDDARSLGAFNL--HKPVENFYQSCDAMLVVGSRLRGNETLKYELKLPRPLYRVDADP 311 D +G L P + + + ML++GS +E L + K + ++D Sbjct: 240 ASYDHPHWIGGVGLIGGAPGTDALHAAEVMLMLGSDYPYSEFLPTKSK----VIQIDERG 295 Query: 312 AAEGRCYTSDYFVCGDSALVLGGLADRLAALQVDPAFMADLKQARESAVHGLLDGLGPYG 371 GR + G A + L +R+ + D AF+ RE + LD Sbjct: 296 FVLGRRAPVTLGITGSVAPAVQQLLERVEG-KTDGAFLQQANAHRER-WNAKLDSAAEIK 353 Query: 372 TLVEQLQ-QAAGRQFN--------WVRDVTVSNSTWGNRYLRIFDPRAGVHALGGGIGQG 422 ++++ Q+ R + +V D V + W ++R R G L Sbjct: 354 RHHKKIKPQSVARAISDLADDDAAFVVDTGVV-TLWCGNWIR---QRGGQRILASFNNAA 409 Query: 423 LAMGIGAAIGAAVTQSGKKTFTLAGDGGFILNLGELATAVQEQADMVIVLMNDQGYGVIK 482 + +G A G ++ GDGGF + LGE T+V+ + + +V+ N++ +G++ Sbjct: 410 VGTSLGQANGIQALDRERQVVVAVGDGGFTMLLGEFMTSVEHRLPVKVVVFNNREWGLVH 469 Query: 483 NIQDAQYGGRRHYVDLHTPDYAALSAALKLRHKRVGDLSQVAAGLDEALSQQGPFLLEI 541 + + PD+AA + A + V ++ GL + L+ GP +L++ Sbjct: 470 LEMEEAGMPAFEGSEFPNPDFAAFARACGGQGFSVRTPEELEGGLQQLLAAPGPAVLDV 528 >gi|300311743|ref|YP_003775835.1| acetolactate synthase [Herbaspirillum seropedicae SmR1] Length = 625 Score = 79.7 bits (195), Expect = 5e-16, Method: Compositional matrix adjust. Identities = 138/553 (24%), Positives = 233/553 (42%), Gaps = 56/553 (10%) Query: 36 GVISIHNMPILDAFGE-----RGKIRFIPARGEAGGANMADAYART--TGGLGVCLTSTG 88 GV SI + GE R + A E A+ A AYA+ + TS G Sbjct: 40 GVFSIFGHGNVAGMGEALYQYRATLPTYRAHNEQAMAHSAIAYAKAHLRQRMMAVTTSIG 99 Query: 89 TAAGNAAGAMVEALTAGTPLLHLTGQI-----ETPYLDQSLAYIHEAPDQLSMLKAISKA 143 A N A A P+L L G + P L Q + + +S+ Sbjct: 100 PGATNLLTAAALAHVNRLPVLLLPGDVFVSRRPDPVLQQLEDFNDPGLSVNDAFRPLSRM 159 Query: 144 AFRIRSVETAISTIKLAVQTALTPPM-GPVSVEIPIDIQAAMISMPTN-LQPLAIPP--S 199 RI E ++ + A+Q P GPV++ +P D+Q P + P I P Sbjct: 160 FDRICYPEQLLTALPRAIQVLTDPAQCGPVTLALPQDVQTMAYDYPLDFFSPRVIVPRAQ 219 Query: 200 LPSARALDELAERLLKARRPLLWLGGGARHAGP-QVQRLLALGFGV-VTSTQ-GRGIVPE 256 P+A+ L++ L +AR+PLL GGG + + R A GV V TQ G+ +P Sbjct: 220 APAAQELEDAVALLRQARQPLLIAGGGVLYGNACEHLRRFAEQHGVPVAETQAGKSALPW 279 Query: 257 DDARSLGAFNLH-KPVEN-FYQSCDAMLVVGSRLR----GNETLKYELKLPRPLYRVDAD 310 A +GA + P N Q D ++ VG+RL+ G+ TL + +L V+A Sbjct: 280 SHALQMGAIGVTGSPAANALAQEADVVIAVGTRLQDFTTGSHTLFAQARLLN--LNVNAM 337 Query: 311 PAAEGRCYTSDYFVCGDSALVLGGLADRLAALQVDPAFMA-------DLKQARESAVHGL 363 A + R + D+ L L L+ L + P + A D ++ R + + G Sbjct: 338 DAHKWRGLA----LQADAGLGLEALSHALEGWRSAPEWHARAHTLAQDWRE-RVAQITGQ 392 Query: 364 LD--GLGPY-GTLVEQLQQAAGRQFNWVRDVTVSNS-TWGNRYLRIFDP-RAGVHALGGG 418 D G PY G ++ +Q++ + +D+ V + T +++ R G + + G Sbjct: 393 TDTGGRLPYDGEVIGAIQRSVADSPS--QDIVVCAAGTLPAELHKLWRAGRPGAYHVEYG 450 Query: 419 IGQGLAMGIGAAIGAAVTQSGKKTFTLAGDGGFILNLGELATAVQEQADMVIVLMNDQGY 478 + + +G + Q ++ + GDG +++ ELAT+V A +++V+++++GY Sbjct: 451 YS-CMGYEVAGGLGVKLAQPQREVIVIVGDGSYLMMNSELATSVMLGAKLIVVVLDNRGY 509 Query: 479 GVIKNIQDAQYGG--RRHYVDLHTP-------DYAALSAALKLRHKRVGDLSQVAAGLDE 529 G I +Q A G D D+AA + +L + V ++++ A L Sbjct: 510 GCINRLQQACGGAPFNNMLADCLQAGPGAPAIDFAAHARSLGALGENVKTITELEAALQR 569 Query: 530 ALSQQGPFLLEID 542 A + +L+ ID Sbjct: 570 ARAADRSYLVCID 582 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,664,829 Number of extensions: 66557 Number of successful extensions: 232 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 229 Number of HSP's successfully gapped: 3 Length of query: 571 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 481 Effective length of database: 1,193,720 Effective search space: 574179320 Effective search space used: 574179320 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)