BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1294.p (274 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herb... 102 4e-23 gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein... 100 1e-22 gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 97 1e-21 gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxido... 91 1e-19 gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reduc... 86 2e-18 >gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 102 bits (253), Expect = 4e-23, Method: Compositional matrix adjust. Identities = 89/271 (32%), Positives = 131/271 (48%), Gaps = 27/271 (9%) Query: 3 MTSLQGKVAVVTGGSSGIGLASVELLLEAGAAVAFCGRDSARLKEAEAALRQRYPRASLY 62 M +LQGKVA++TG S+GIG A+ L GAA+ R L++ A + + RAS+ Sbjct: 1 MNTLQGKVALITGASAGIGRATALLFARHGAALILTARRQDALEQVAALICAQGGRASVV 60 Query: 63 SAPCDVLAQQQVQAFANAVENTLGPVSILVNNAGQ-GRVSTFADTSDDAWSEELHLKFFS 121 + A + + A A++ G + I +NNAG G A+ S W + LH S Sbjct: 61 AGDVGEAATHE-RGVAMALDE-FGGLDIAINNAGAVGACKPLAEISPQQWQDTLHANLTS 118 Query: 122 VIYPTRAFLPQLQAMSARHGDAAIVCVNSLLATQPE-PHMVATSAARAGVHNLVRSLATE 180 R +P AM AR G AIV +S + + P M A AA+AG+ LV+ +A + Sbjct: 119 AFLGARCQIP---AMLAR-GAGAIVFTSSFVGSSAGLPGMAAYGAAKAGLMGLVKGIAAD 174 Query: 181 LATGPRACVRVNGILIGLVESGQWRRRYAARDEALQDQTWAQWSAALAEKKHIQLGRLGL 240 A +R N +L G V++ + D QW+A L K R+ Sbjct: 175 YAV---QGIRANALLPGGVDT-----------DMGGDPQQKQWAAGLHAMK-----RIAQ 215 Query: 241 PREAAQAIVFLASPLSSYTTGSHIDVSGGLS 271 P E A A +FLASP++S+ TG+ + GG S Sbjct: 216 PEEIAAAALFLASPMASFVTGTALYADGGNS 246 >gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 100 bits (250), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 91/275 (33%), Positives = 138/275 (50%), Gaps = 35/275 (12%) Query: 6 LQGKVAVVTGGS--SGIGLASVELLLEAGAAVAFCGRDSARLKEAEAALRQRYPRASLYS 63 L+ KVA++TGG+ +G+G ++ L+ GA + D R + A AA R L + Sbjct: 3 LKDKVAIITGGAGQNGLGFSTARLMAAQGARIVIL--DLERAEPAGAAARLGAQHLGLVA 60 Query: 64 APCDVLAQQQVQAFANAVENTLGPVSILVNNAGQGRVSTFADTSDDAWSEELHLKFFSVI 123 DV + +A A AV G + ILVNNAG + + + + + L + + Sbjct: 61 ---DVTDKAACEAAAAAVLKQFGRIDILVNNAGVTQPAKLLEITGKDYDRILDVSLRGTL 117 Query: 124 YPTRAFLPQLQAMSARHGDAAIVCVNSLLATQPE-----PHMVATSAARAGVHNLVRSLA 178 Y ++A LP +QA + +IVC++S+ A + PH SAA+AGV L R++A Sbjct: 118 YMSQAVLPAMQAQQS----GSIVCISSVSAQRGGGILGGPHY---SAAKAGVLGLARAMA 170 Query: 179 TELATGPRACVRVNGILIGLVESGQWRRRYAARDEALQDQTWAQWSAALAEKKHIQLGRL 238 E GP+ +RVN I GL+ + + +D A+ A +A+ I LGRL Sbjct: 171 REF--GPQG-IRVNCITPGLIST-----------DINKDILSAEKKAEIAQT--IPLGRL 214 Query: 239 GLPREAAQAIVFLASPLSSYTTGSHIDVSGGLSRH 273 G P + VFLAS LS Y TG +DV+GG+ H Sbjct: 215 GGPDDVGGVCVFLASDLSQYCTGVTLDVNGGMLIH 249 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 97.1 bits (240), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 91/273 (33%), Positives = 135/273 (49%), Gaps = 28/273 (10%) Query: 1 MAMTSLQGKVAVVTGGSSGIGLASVELLLEAGAAV---AFCGRDSARLKEAEAALRQRYP 57 M+ +LQG+VA+VTG S GIG A L GA V A +A + E AAL Sbjct: 1 MSAQNLQGQVALVTGASRGIGRAIATELARQGAKVIGTATSESGAAAITEYLAALGPEAG 60 Query: 58 RASLYSAPCDVLAQQQVQAFANAVENTLGPVSILVNNAGQGRVSTFADTSDDAWSEELHL 117 R ++ +V + A V+ G +SILVNNAG + D+ W + Sbjct: 61 RGAV----LNVNDAEASVALVEQVQKDFGSLSILVNNAGITQDQLAMRMKDEEWDSVIAT 116 Query: 118 KFFSVIYPTRAFLPQLQAMSARHGDAAIVCVNSLLATQPEPHMVATSAARAGVHNLVRSL 177 +V +RA L + M AR G I+ + S++ P + +AA+AGV + R+L Sbjct: 117 NLTAVGRLSRAVLRGM--MKARTGR--IINITSVVGEAGNPGQMNYAAAKAGVAGMSRAL 172 Query: 178 ATELATGPRACVRVNGILIGLVESGQWRRRYAARDEALQDQTWAQWSAALAEKKHIQLGR 237 A E+ G R + VN + G +++ R AL +Q Q +A L + I LGR Sbjct: 173 AREI--GSR-NITVNCVAPGFIDTDMTR--------ALNEQ---QSAAIL---QQIPLGR 215 Query: 238 LGLPREAAQAIVFLASPLSSYTTGSHIDVSGGL 270 LG E A A+ FLASP ++Y TG+ ++V+GG+ Sbjct: 216 LGAAEEIAYAVAFLASPQAAYITGTTLNVNGGM 248 >gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 264 Score = 90.9 bits (224), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 73/272 (26%), Positives = 125/272 (45%), Gaps = 31/272 (11%) Query: 5 SLQGKVAVVTGGSSGIGLASVELLLEAGAAVAFCGRDSARLKEAEAALRQRYPRASLYSA 64 SL+GK+A++TG S GIGLA + +AGAA+ GRD+ +L A LR +++ + Sbjct: 16 SLEGKLALITGSSGGIGLALARGMAQAGAAIVLNGRDAGKLAAVAAQLRAE--GHTIHES 73 Query: 65 PCDVLAQQQVQAFANAVENTLGPVSILVNNAGQGRVSTFADTSDDAWSEELHLKFFSVIY 124 D + ++V+A +E+ +GP+ ILVNNAG R + + + W + + SV + Sbjct: 74 SFDATSSEEVKAAVQKIESEIGPIGILVNNAGIQRRNPLEEFKESDWHDLISTNLNSVFF 133 Query: 125 PTRAFLPQLQAMSARH----GDAAIVCVNSLLATQPEPHMVATSAARAGVHNLVRSLATE 180 +A ARH G I+ + S+ + P + +A + V + +A + Sbjct: 134 AAQAV--------ARHMIPRGAGKIINICSVQSELGRPGIAPYTATKGAVKMFTKGMAID 185 Query: 181 LATGPRACVRVNGILIGLVESGQWRRRYAARDEAL-QDQTWAQWSAALAEKKHIQLGRLG 239 + ++VNG+ G ++ + AL D + W + R G Sbjct: 186 WG---KYGIQVNGLGPGYFKT--------EMNMALVNDAKFTDWLVGRTPSR-----RWG 229 Query: 240 LPREAAQAIVFLASPLSSYTTGSHIDVSGGLS 271 + A VFLAS S + G + V GG++ Sbjct: 230 DVEDLVGAAVFLASDASRFVNGHILYVDGGVT 261 >gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 261 Score = 86.3 bits (212), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 78/268 (29%), Positives = 117/268 (43%), Gaps = 24/268 (8%) Query: 3 MTSLQGKVAVVTGGSSGIGLASVELLLEAGAAVAFCGRDSARLKEAEAALRQRYPRASLY 62 + +GKV +VTG +SGIG A+ + GA V RD+A L + +L P Sbjct: 9 LQRFEGKVVIVTGAASGIGEATARRFSDEGARVLLADRDAAALGKVFDSL----PPERTA 64 Query: 63 SAPCDVLAQQQVQAFANAVENTLGPVSILVNNAGQGRVSTFADTSDDAWSEELHLKFFSV 122 + DV +QV+ + G + +LV++AG + S + W V Sbjct: 65 ARETDVSHHEQVRQLVDFAIERFGQLDVLVSDAGVFAEGNVTEVSPEDWHRVQATNVNGV 124 Query: 123 IYPTRAFLPQLQAMSARHGDAAIVCVNSLLATQPEPHMVATSAARAGVHNLVRSLATELA 182 Y R LP L+ IV V S+ + ++ A +A++ V NL R++A + Sbjct: 125 FYGAREALPHLEKTR-----GCIVNVASVSGLAADWNLSAYNASKGAVCNLTRAMALDFG 179 Query: 183 TGPRACVRVNGILIGLVESGQWRRRYAARDEALQDQTWAQWSAALAEKKHIQLGRLGLPR 242 R VR+N + L + A D L D+ AE+ I LGR P Sbjct: 180 ---RKGVRINAVCPSLTHTAMTAD--MADDPPLLDK--------FAER--IALGRGADPL 224 Query: 243 EAAQAIVFLASPLSSYTTGSHIDVSGGL 270 E A I FLASP +S+ G ++ V GGL Sbjct: 225 EIAAVITFLASPDASFVNGVNLPVDGGL 252 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.129 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 776,941 Number of extensions: 28428 Number of successful extensions: 231 Number of sequences better than 1.0e-08: 38 Number of HSP's gapped: 194 Number of HSP's successfully gapped: 38 Length of query: 274 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 190 Effective length of database: 1,222,130 Effective search space: 232204700 Effective search space used: 232204700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)