BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1288.p (331 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309986|ref|YP_003774078.1| nitrate/sulfonate/bicarbonate A... 59 5e-10 >gi|300309986|ref|YP_003774078.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 328 Score = 58.9 bits (141), Expect = 5e-10, Method: Compositional matrix adjust. Identities = 71/280 (25%), Positives = 128/280 (45%), Gaps = 24/280 (8%) Query: 47 FAPWVLAQHKGYYADENLK-----VSFIVAKGGVDVAKQIGAGNAVIGGAIGDTPIVVRA 101 ++P L K + +E+LK V +++++G + + + + G G ++ RA Sbjct: 42 YSPPSLVLRKFGWLEEDLKAQGTEVKWVLSQGSNRALEYLNSDSVDFGSTAGLAALLARA 101 Query: 102 NGIPVKAIAVLGAGSVTMVAANPADGIKSLKDLKGKTVTVMSYTDTTYYAFLGSLKTAGL 161 NG PVKA+ + T +A +KS+ +LKGK + TD Y L +L GL Sbjct: 102 NGNPVKAVYIYSRPEWTALAVGKESPVKSINELKGKKIAATKGTD-PYLFLLRALHENGL 160 Query: 162 SRTDLQI----QAAGPAGVWQLYTSGKSQAFAGV-PDWVVNASDAGAKLELLDNGGFKSM 216 ++D++I A G A + Q G+ A+AG+ P + +AG++L + N F + Sbjct: 161 KKSDVEIVHLQHADGRAALEQ----GRVAAWAGLDPHLAASELEAGSRL-IYRNVNFNTY 215 Query: 217 AQAILASDDTIKKNPELLKRLVRATLRGMQDIMKDPKAAADDYVAALPSYKGKEASIQAT 276 + SD ++P+ +KR++ A R I+ P D AL S + K ++ A Sbjct: 216 G-FLNVSDSFAARHPDQVKRVIAAYERARSWIIAHP-----DETVALLSEESKLSAPVAR 269 Query: 277 FEMYNKYVYANQKTLGQIDAARMEAVQKFYVSEGIVAKAT 316 ++ K +Q +G+ + A + E +V T Sbjct: 270 LQL--KRNDFSQPQIGREHIEALRAAAPILLEEDLVKSGT 307 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.131 0.369 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 808,513 Number of extensions: 30012 Number of successful extensions: 152 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 160 Number of HSP's successfully gapped: 4 Length of query: 331 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 245 Effective length of database: 1,212,660 Effective search space: 297101700 Effective search space used: 297101700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)