BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1280.p (259 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312114|ref|YP_003776206.1| 3-oxoacyl-[acyl-carrier-protein... 404 e-114 gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herb... 114 8e-27 gi|300313237|ref|YP_003777329.1| dehydrogenase [Herbaspirillum s... 97 2e-21 gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herb... 93 2e-20 gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxido... 90 2e-19 >gi|300312114|ref|YP_003776206.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 262 Score = 404 bits (1039), Expect = e-114, Method: Compositional matrix adjust. Identities = 213/262 (81%), Positives = 234/262 (89%), Gaps = 3/262 (1%) Query: 1 MDLGISGRRALVCASSKGLGLACAISLAREGVAVTMTARGAEVLEAAAQQVRA---ATGA 57 MDL ++G+ ALVCASSKGLG ACA SLAREGV VTM ARG E LEAAAQ++RA ATGA Sbjct: 1 MDLRLNGKTALVCASSKGLGRACAHSLAREGVHVTMLARGREALEAAAQEIRAEVAATGA 60 Query: 58 TVTTVAGDITTPEGRALALAACPAPDILVTNAGGPKPGDFREWSREDWIAAIDANMLTPI 117 +TTV+ DITTPEGRALALAACPAPDILVTNAGGPKPGDFR+WSREDWIAA+DANMLTPI Sbjct: 61 IITTVSCDITTPEGRALALAACPAPDILVTNAGGPKPGDFRDWSREDWIAAVDANMLTPI 120 Query: 118 ALIKATVDGMMQRGFGRIVNITSGAVKAPIDILGLSNGARSGLTGFVAGLARTTVAHNVT 177 LIKATVDGM+ R FGRIVNITS AV+APIDILGLSNGARSGLTGFVAGLAR TVAHNVT Sbjct: 121 ELIKATVDGMIARRFGRIVNITSSAVRAPIDILGLSNGARSGLTGFVAGLARKTVAHNVT 180 Query: 178 INNLLPGPFETERLLTTAAASARGSGKSTEQVLAARRAANPARRFGQPAEFGDACAWLCS 237 INNLLPGPFET+RL TA+ +A+ SG+S +++LA RR NPA RFG PAEFGDACA+LCS Sbjct: 181 INNLLPGPFETDRLRATASGAAQESGRSVDEILAERRKLNPAGRFGDPAEFGDACAYLCS 240 Query: 238 AQAGFVTGQNLLMDGGAYPGTF 259 AQAGF+TGQNLLMDGGAYPGTF Sbjct: 241 AQAGFITGQNLLMDGGAYPGTF 262 >gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 246 Score = 114 bits (285), Expect = 8e-27, Method: Compositional matrix adjust. Identities = 78/264 (29%), Positives = 122/264 (46%), Gaps = 29/264 (10%) Query: 5 ISGRRALVCASSKGLGLACAISLAREGVAVTM-----TARGAEVLEAAAQQVRAATGATV 59 +S R A V G+G A L ++G V + R + L +Q G + Sbjct: 1 MSKRIAYVTGGMGGIGTAICTRLCKDGYTVVAGCGPNSTRKDKWLATMREQ-----GFDI 55 Query: 60 TTVAGDITTPEGRALALAACPAP----DILVTNAGGPKPGDFREWSREDWIAAIDANMLT 115 G+++ E A A D+LV NAG + G FR+ S++DW A ID N+ + Sbjct: 56 HASEGNVSNWESTKAAFEKVRAEIGEVDVLVNNAGITRDGQFRKMSKDDWDAVIDTNLNS 115 Query: 116 PIALIKATVDGMMQRGFGRIVNITSGAVKAPIDILGLSN--GARSGLTGFVAGLARTTVA 173 + K ++GM +RGFGRI+NI+S V G +N A++G+ GF LA+ Sbjct: 116 LFNVTKQVIEGMCERGFGRIINISS--VNGQKGQFGQTNYSTAKAGIHGFTMALAQEVAT 173 Query: 174 HNVTINNLLPGPFETERLLTTAAASARGSGKSTEQVLAARRAANPARRFGQPAEFGDACA 233 VT+N + PG T+ + +VL + P +R G+P E A Sbjct: 174 KGVTVNTVSPGYIGTDMVRAIRP-----------EVLEKIVSGIPVKRLGEPEEIASIVA 222 Query: 234 WLCSAQAGFVTGQNLLMDGGAYPG 257 W+ S++ G+ TG + ++GG Y G Sbjct: 223 WIASSEGGYATGSDFSLNGGLYMG 246 >gi|300313237|ref|YP_003777329.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 264 Score = 96.7 bits (239), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 83/266 (31%), Positives = 122/266 (45%), Gaps = 10/266 (3%) Query: 1 MDLGISGRRALVCASSKGLGLACAISLAREGVAVTMTARGAEVLEAAAQQVRAATGATVT 60 M+L + G+ A+V S G+G A A +LAREGV V + AR LE A + +TG V Sbjct: 1 MELKLQGKAAVVTGGSLGIGRAVAEALAREGVRVAIVARSKGKLEEVASALSQSTGTEVI 60 Query: 61 TVAGDITTPEGRALALAACPAP----DILVTNA---GGPKPGDFREWSREDWIAAIDANM 113 VA D++ E A+A DILV A GG + + S E + I+ + Sbjct: 61 AVAADVSNTEQVEAAVAQAAQHFGRIDILVNGAAHPGGLVRSEIQHASPEGLLEDINIKV 120 Query: 114 LTPIALIKATVDGMMQRGFGRIVNITSGAVKAPIDILGLSNGARSGLTGFVAGLARTTVA 173 + + KA M G+GRI+NI + + G+ N A LT L+ Sbjct: 121 VGYMRFAKAVAPHMRAGGYGRIINIGGLTGRGSKQLSGMRNVAICHLT---KTLSDQLGP 177 Query: 174 HNVTINNLLPGPFETERLLTTAAASARGSGKSTEQVLAARRAANPARRFGQPAEFGDACA 233 +T+N + PG ET + A+ G + EQV A P RR Q E D Sbjct: 178 DGITVNVIHPGVVETPHIHELYEKEAKLQGLTPEQVEQNYAKATPIRRVLQVEEIADVVL 237 Query: 234 WLCSAQAGFVTGQNLLMDGGAYPGTF 259 +L S +A +TG+++ +DGG G + Sbjct: 238 FLASPRAAAITGESIAVDGGITRGIY 263 >gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 93.2 bits (230), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 84/258 (32%), Positives = 118/258 (45%), Gaps = 26/258 (10%) Query: 5 ISGRRALVCASSKGLGLACAISLAREGVAVTMTARGAEVLEAAAQQVRAATGATVTTVAG 64 + G+ AL+ +S G+G A A+ AR G A+ +TAR + LE A + A G + VAG Sbjct: 4 LQGKVALITGASAGIGRATALLFARHGAALILTARRQDALEQVAALI-CAQGGRASVVAG 62 Query: 65 DI----TTPEGRALALAACPAPDILVTNAGGP---KPGDFREWSREDWIAAIDANMLTPI 117 D+ T G A+AL DI + NAG KP E S + W + AN+ + Sbjct: 63 DVGEAATHERGVAMALDEFGGLDIAINNAGAVGACKP--LAEISPQQWQDTLHANLTSAF 120 Query: 118 ALIKATVDGMMQRGFGRIVNITSGAVKAPIDILGLS--NGARSGLTGFVAGLARTTVAHN 175 + + M+ RG G IV TS V + + G++ A++GL G V G+A Sbjct: 121 LGARCQIPAMLARGAGAIV-FTSSFVGSSAGLPGMAAYGAAKAGLMGLVKGIAADYAVQG 179 Query: 176 VTINNLLPGPFETERLLTTAAASARGSGKSTEQVLAARRAANPARRFGQPAEFGDACAWL 235 + N LLPG +T+ G +Q A A +R QP E A +L Sbjct: 180 IRANALLPGGVDTD----------MGGDPQQKQWAAGLHA---MKRIAQPEEIAAAALFL 226 Query: 236 CSAQAGFVTGQNLLMDGG 253 S A FVTG L DGG Sbjct: 227 ASPMASFVTGTALYADGG 244 >gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 264 Score = 89.7 bits (221), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 75/257 (29%), Positives = 119/257 (46%), Gaps = 18/257 (7%) Query: 3 LGISGRRALVCASSKGLGLACAISLAREGVAVTMTARGAEVLEAAAQQVRAATGATVTTV 62 + G+ AL+ SS G+GLA A +A+ G A+ + R A L A A Q+R A G T+ Sbjct: 15 FSLEGKLALITGSSGGIGLALARGMAQAGAAIVLNGRDAGKLAAVAAQLR-AEGHTIHES 73 Query: 63 AGDITTPEGRALALAACPAP----DILVTNAGGPKPGDFREWSREDWIAAIDANMLTPIA 118 + D T+ E A+ + ILV NAG + E+ DW I N+ + Sbjct: 74 SFDATSSEEVKAAVQKIESEIGPIGILVNNAGIQRRNPLEEFKESDWHDLISTNLNSVFF 133 Query: 119 LIKATVDGMMQRGFGRIVNITSGAVKAPIDILGLS--NGARSGLTGFVAGLARTTVAHNV 176 +A M+ RG G+I+NI S V++ + G++ + + F G+A + + Sbjct: 134 AAQAVARHMIPRGAGKIINICS--VQSELGRPGIAPYTATKGAVKMFTKGMAIDWGKYGI 191 Query: 177 TINNLLPGPFETERLLTTAAASARGSGKSTEQVLAARRAANPARRFGQPAEFGDACAWLC 236 +N L PG F+TE + K T+ ++ P+RR+G + A +L Sbjct: 192 QVNGLGPGYFKTEMNMALV-----NDAKFTDWLV----GRTPSRRWGDVEDLVGAAVFLA 242 Query: 237 SAQAGFVTGQNLLMDGG 253 S + FV G L +DGG Sbjct: 243 SDASRFVNGHILYVDGG 259 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.132 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 791,973 Number of extensions: 30188 Number of successful extensions: 246 Number of sequences better than 1.0e-08: 33 Number of HSP's gapped: 201 Number of HSP's successfully gapped: 33 Length of query: 259 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 176 Effective length of database: 1,226,865 Effective search space: 215928240 Effective search space used: 215928240 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)