BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1272.p (551 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310786|ref|YP_003774878.1| dihydrolipoamide acetyltransfer... 920 0.0 gi|300312272|ref|YP_003776364.1| dihydrolipoamide succinyltransf... 184 1e-47 gi|300310787|ref|YP_003774879.1| 2-oxoglutarate dehydrogenase E3... 112 7e-26 >gi|300310786|ref|YP_003774878.1| dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex [Herbaspirillum seropedicae SmR1] Length = 554 Score = 920 bits (2379), Expect = 0.0, Method: Compositional matrix adjust. Identities = 536/554 (96%), Positives = 539/554 (97%), Gaps = 3/554 (0%) Query: 1 MSQVEVKVPDIGDFKEVEVIEVMVKVGDTIKVDQSLITVESDKASMEIPSSQAGVVKEIK 60 MSQVEVKVPDIGDFKEVEVIEVMVKVGDTIKVDQSLITVESDKASMEIPSSQAGVVKEIK Sbjct: 1 MSQVEVKVPDIGDFKEVEVIEVMVKVGDTIKVDQSLITVESDKASMEIPSSQAGVVKEIK 60 Query: 61 VKVGDKVAEGSLLVIVEGEGAAAAPAAAPQAAAAASAAAQASAPAPAPVAAPAAAAGGTV 120 VKVGDKVAEGSLLVIVEGEGAAAAPAAAPQAAAAA AAAQA+APAPAP AAPAAAAGGTV Sbjct: 61 VKVGDKVAEGSLLVIVEGEGAAAAPAAAPQAAAAAPAAAQAAAPAPAPAAAPAAAAGGTV 120 Query: 121 EIEVPDIGDFKEVEVIEVMVKVGDTVKAEQSLLTVESDKASMEIPSSHAGVIKELKVKLG 180 EIEVPDIGDFKEVEVIEVMVKVGDTVKAEQSLLTVESDKASMEIPSSHAGVIKELKVKLG Sbjct: 121 EIEVPDIGDFKEVEVIEVMVKVGDTVKAEQSLLTVESDKASMEIPSSHAGVIKELKVKLG 180 Query: 181 DKVSKGSIIATIEAAGGAPAAAPAAAPAAAPAAAPAPAAAPAPAAAAVPAIATA---SAT 237 DKVSKGSIIATIEAAGGAPAAAPAAAPAAAPAAA APA A APA AA A+ SAT Sbjct: 181 DKVSKGSIIATIEAAGGAPAAAPAAAPAAAPAAAAAPAPASAPAPAAAAAVPAIATASAT 240 Query: 238 STGGKAHASPSVRKFARELGVDLSRVPATGPKGRILQLDVQNFVKGVMAGSTSVAASAPA 297 STGGKAHASPSVRKFARELGVDLSRVPATGPKGRILQLDVQNFVKGVMAGSTSVAASAPA Sbjct: 241 STGGKAHASPSVRKFARELGVDLSRVPATGPKGRILQLDVQNFVKGVMAGSTSVAASAPA 300 Query: 298 TNGSGAGMNLLPWPSLDFSKFGETELQALSRIKKISGPNLHRNWVMIPHVTQFDEADVTE 357 TNGSGAGMNLLPWPSLDFSKFGETELQ LSRIKKISGPNLHRNWVMIPHVTQFDEADVTE Sbjct: 301 TNGSGAGMNLLPWPSLDFSKFGETELQPLSRIKKISGPNLHRNWVMIPHVTQFDEADVTE 360 Query: 358 LEEFRKTSNDAFAKAKSPVKLTMLAFVIKASVSALKKFPAFNSSLDAKGENLILKKFYNI 417 LEEFRKTSNDAFAKAKSPVKLTMLAFVIKASVSALKKFPAFNSSLDAKGENLILKKFYNI Sbjct: 361 LEEFRKTSNDAFAKAKSPVKLTMLAFVIKASVSALKKFPAFNSSLDAKGENLILKKFYNI 420 Query: 418 GFAADTPNGLVVPVIKNADQKSIGQIAIEMGELSAQARDGKLKPADMQGATFTISSLGGI 477 GFAADTPNGLVVPVIKNADQKSIGQIAIEMGELSAQARDGKLKPADMQGATFTISSLGGI Sbjct: 421 GFAADTPNGLVVPVIKNADQKSIGQIAIEMGELSAQARDGKLKPADMQGATFTISSLGGI 480 Query: 478 GGTAFTPIINAPEVAILGLSKSITKPVWDGKQFVPRLMLPTSLSYDHRVIDGAMGARFSA 537 GGTAFTPIINAPEVAILGLSKSITKPVWDGKQFVPRLMLPTSLSYDHRVIDGAMGARFSA Sbjct: 481 GGTAFTPIINAPEVAILGLSKSITKPVWDGKQFVPRLMLPTSLSYDHRVIDGAMGARFSA 540 Query: 538 YLAEVLGDLRKSLL 551 YLA+VLGDLRKSLL Sbjct: 541 YLADVLGDLRKSLL 554 >gi|300312272|ref|YP_003776364.1| dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex protein [Herbaspirillum seropedicae SmR1] Length = 413 Score = 184 bits (468), Expect = 1e-47, Method: Compositional matrix adjust. Identities = 137/433 (31%), Positives = 217/433 (50%), Gaps = 26/433 (6%) Query: 120 VEIEVPDIGD-FKEVEVIEVMVKVGDTVKAEQSLLTVESDKASMEIPSSHAGVIKELKVK 178 +E++VP + + E +++ KVG+ V +++L+ +E+DK +E+PS AGVI ++ Sbjct: 4 IEVKVPQLSESVAEATLLQWHKKVGEPVSRDENLIDIETDKVVLELPSPDAGVITQIIKA 63 Query: 179 LGDKVSKGSIIATIEAAGGAPAAAPAAAPAAAPAAAPAPAAAPAPAAAAVPAIATASATS 238 G V G +IA ++ A AP AAPAP A P A P +A+ Sbjct: 64 DGATVVAGEVIAILDTDASA-----QVAPTEV-KAAPAPQATNEPTPVAAPELASKGDV- 116 Query: 239 TGGKAHASPSVRKFARELGVDLSRVPATGPKGRILQLDVQNFVKGVMAGSTSVAASAPAT 298 A P+ K + + S+V TG GR+ + DV + A + A A A Sbjct: 117 ------AMPAAAKLLADNNLSTSQVTGTGKDGRVTKGDVLGALSAPAAAPAAAAKPALAQ 170 Query: 299 NGSGAGMNLLPWPSLDFSKFGETELQALSRIKKISGPNLHRNWVMIPHVTQFDEADVTEL 358 + L P + +SR++ L ++ +T F+E ++ + Sbjct: 171 VAAPVKAGLESRPE---------QRVPMSRLRARVAERLVQSQSTNAILTTFNEINMQPV 221 Query: 359 EEFRKTSNDAFAKAKSPVKLTMLAFVIKASVSALKKFPAFNSSLDAKGENLILKKFYNIG 418 + R D F K VKL ++F +KA V ALKK+P N+S+D G +++ +++IG Sbjct: 222 IDLRNKYKDKFEKEHG-VKLGFMSFFVKAVVHALKKYPIVNASVD--GNDIVYHGYFDIG 278 Query: 419 FAADTPNGLVVPVIKNADQKSIGQIAIEMGELSAQARDGKLKPADMQGATFTISSLGGIG 478 A +P GLVVP++++ADQ SI I ++GE +A+DGKL ++ G TFT+S+ G G Sbjct: 279 VAVGSPRGLVVPILRDADQMSIADIEKKIGEFGQKAKDGKLSIEELSGGTFTVSNGGTFG 338 Query: 479 GTAFTPIINAPEVAILGLSKSITKPVWDGKQFVPRLMLPTSLSYDHRVIDGAMGARFSAY 538 TPIIN P+ AILG+ + + V + Q V R + +LSYDHR+IDG Sbjct: 339 SMLSTPIINPPQSAILGIHATKERAVVENGQVVVRPINYFALSYDHRIIDGREAVLSLVA 398 Query: 539 LAEVLGDLRKSLL 551 + E L D + LL Sbjct: 399 IKEALEDPARLLL 411 Score = 63.5 bits (153), Expect = 4e-11, Method: Compositional matrix adjust. Identities = 42/121 (34%), Positives = 68/121 (56%), Gaps = 6/121 (4%) Query: 1 MSQVEVKVPDIGD-FKEVEVIEVMVKVGDTIKVDQSLITVESDKASMEIPSSQAGVVKEI 59 M+Q+EVKVP + + E +++ KVG+ + D++LI +E+DK +E+PS AGV+ +I Sbjct: 1 MAQIEVKVPQLSESVAEATLLQWHKKVGEPVSRDENLIDIETDKVVLELPSPDAGVITQI 60 Query: 60 KVKVGDKVAEGSLLVIVEGEGAAAAPAAAPQAAAAASAAAQASAPAPAPVAAPAAAAGGT 119 G V G ++ I++ + +A +AA A A + P PVAAP A+ G Sbjct: 61 IKADGATVVAGEVIAILDTDASAQVAPTEVKAAPAPQATNE-----PTPVAAPELASKGD 115 Query: 120 V 120 V Sbjct: 116 V 116 >gi|300310787|ref|YP_003774879.1| 2-oxoglutarate dehydrogenase E3 component lipoamide dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 596 Score = 112 bits (280), Expect = 7e-26, Method: Compositional matrix adjust. Identities = 73/80 (91%), Positives = 76/80 (95%) Query: 1 MSQVEVKVPDIGDFKEVEVIEVMVKVGDTIKVDQSLITVESDKASMEIPSSQAGVVKEIK 60 MS EVKVPDIGDFKEVEVIEVMVKVGDTIKVDQSLITVESDKASMEIPSSQAGVVKE+K Sbjct: 1 MSLSEVKVPDIGDFKEVEVIEVMVKVGDTIKVDQSLITVESDKASMEIPSSQAGVVKEVK 60 Query: 61 VKVGDKVAEGSLLVIVEGEG 80 VKVGDKVAEGSLL++VE G Sbjct: 61 VKVGDKVAEGSLLLLVEAAG 80 Score = 97.4 bits (241), Expect = 3e-21, Method: Compositional matrix adjust. Identities = 59/76 (77%), Positives = 72/76 (94%) Query: 121 EIEVPDIGDFKEVEVIEVMVKVGDTVKAEQSLLTVESDKASMEIPSSHAGVIKELKVKLG 180 E++VPDIGDFKEVEVIEVMVKVGDT+K +QSL+TVESDKASMEIPSS AGV+KE+KVK+G Sbjct: 5 EVKVPDIGDFKEVEVIEVMVKVGDTIKVDQSLITVESDKASMEIPSSQAGVVKEVKVKVG 64 Query: 181 DKVSKGSIIATIEAAG 196 DKV++GS++ +EAAG Sbjct: 65 DKVAEGSLLLLVEAAG 80 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.312 0.128 0.350 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,264,835 Number of extensions: 47610 Number of successful extensions: 196 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 187 Number of HSP's successfully gapped: 6 Length of query: 551 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 461 Effective length of database: 1,193,720 Effective search space: 550304920 Effective search space used: 550304920 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 133 (55.8 bits)