BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0001.1270.p
         (531 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310784|ref|YP_003774876.1| gamma-glutamate-cysteine ligase...   994   0.0  

>gi|300310784|ref|YP_003774876.1| gamma-glutamate-cysteine ligase
           [Herbaspirillum seropedicae SmR1]
          Length = 531

 Score =  994 bits (2571), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 485/531 (91%), Positives = 502/531 (94%)

Query: 1   MTDLLTRRLQQLSQPQHLALLGQGLRGVERETLRVDRQGQLAQTPHPAALGSALTNELIT 60
           MTDLL RRLQQLSQPQHLALLGQGLRG+ERETLRVD QG LA+TPHP ALGSALTNELIT
Sbjct: 1   MTDLLNRRLQQLSQPQHLALLGQGLRGIERETLRVDAQGHLARTPHPPALGSALTNELIT 60

Query: 61  TDYSEALLEFITPAMPDIADALQRLDEIHRFAYPRLAGEFLWNASMPCVLPEEEQIPLAW 120
           TDYSEALLEFITPAMPDIADALQRLDEIHRFAY +L GEFLWNASMPCVLP E++IP+AW
Sbjct: 61  TDYSEALLEFITPAMPDIADALQRLDEIHRFAYAKLEGEFLWNASMPCVLPPEDEIPIAW 120

Query: 121 YGNSHIGMLKHVYRRGLALRYGRTMQCIAGIHYNFSLSEDLWEVLRDEDDTVDPSLSRRD 180
           YGNSHIGMLKHVYRRGLALRYGRTMQCIAGIHYNFSLSEDLWEVLR+ DD+VD SLSRRD
Sbjct: 121 YGNSHIGMLKHVYRRGLALRYGRTMQCIAGIHYNFSLSEDLWEVLREGDDSVDLSLSRRD 180

Query: 181 YQSERYIALIRNFRRYSWLLMYLFGASPAVATNFLRGRPHQLETLSEDTLYLPYGTSLRM 240
           YQSERYIALIRNFRRYSWLLMYLFGASPAVATNFLRGRPHQLETLSEDTLYLPYGTSLRM
Sbjct: 181 YQSERYIALIRNFRRYSWLLMYLFGASPAVATNFLRGRPHQLETLSEDTLYLPYGTSLRM 240

Query: 241 SDLGYQNHAQANITPHYNNLPAYIRSLSHAVSQPYPPYEAIGTKRDGQWVQINTNILQIE 300
           SDLGYQNHAQANITPHYNNLPAYIRSLSHAVS+PYPPYEAIGTKRDGQWVQINTNILQIE
Sbjct: 241 SDLGYQNHAQANITPHYNNLPAYIRSLSHAVSEPYPPYEAIGTKRDGQWVQINTNILQIE 300

Query: 301 NEFYSSIRPKRVINSGERPVQALSARGVQYVEVRCMDIDPFNPLGIGLEAARFLDVFLHF 360
           NEFYSSIRPKRVINSGERPVQALSARGVQYVEVRCMDIDPFNPLGIGLEAARFLDVFLHF
Sbjct: 301 NEFYSSIRPKRVINSGERPVQALSARGVQYVEVRCMDIDPFNPLGIGLEAARFLDVFLHF 360

Query: 361 LALEASCLTSDEEGAENRANFLATVKEGRRPGLQLHCKGESIVLTDWGNQLLDRMAPVAE 420
           LALE+SCLTSDEEGAENRANFLATVKEGRRPGLQLH KG SI L  WG QLLDRMAPVA+
Sbjct: 361 LALESSCLTSDEEGAENRANFLATVKEGRRPGLQLHFKGASIGLKAWGEQLLDRMAPVAD 420

Query: 421 LLDRHLGGSQHRDSLAVQRGKLADPDTTPSARVLREIRAAGNSFPGYTLANSQRQAEELL 480
           LLDR  GGSQHRDSL  QR KLADPD+TPSARVLREIRA+GNSF  YTLA+SQRQA EL+
Sbjct: 421 LLDRQRGGSQHRDSLQAQRAKLADPDSTPSARVLREIRASGNSFADYTLASSQRQAAELM 480

Query: 481 ARPLAAERSAEFDAIAQASLAAQRDIERTQEGDFDTFVAAYRASTLGNIAI 531
           ARPL A  +A+F AIAQASL AQR IERTQEGDFDTFVAAYRASTLGNIAI
Sbjct: 481 ARPLPAATTADFVAIAQASLDAQRAIERTQEGDFDTFVAAYRASTLGNIAI 531


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.320    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,635,074
Number of extensions: 67623
Number of successful extensions: 188
Number of sequences better than 1.0e-08: 1
Number of HSP's gapped: 188
Number of HSP's successfully gapped: 1
Length of query: 531
Length of database: 1,619,870
Length adjustment: 90
Effective length of query: 441
Effective length of database: 1,193,720
Effective search space: 526430520
Effective search space used: 526430520
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 133 (55.8 bits)