BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1253.p (526 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313312|ref|YP_003777404.1| methyl-accepting chemotaxis pro... 328 7e-91 gi|300310695|ref|YP_003774787.1| methyl-accepting chemotaxis sen... 325 8e-90 gi|300312840|ref|YP_003776932.1| methyl-accepting chemotaxis I p... 319 3e-88 gi|300313897|ref|YP_003777989.1| methyl-accepting chemotaxis pro... 316 3e-87 gi|300313820|ref|YP_003777912.1| methyl-accepting chemotaxis tra... 315 4e-87 >gi|300313312|ref|YP_003777404.1| methyl-accepting chemotaxis protein I [Herbaspirillum seropedicae SmR1] Length = 544 Score = 328 bits (841), Expect = 7e-91, Method: Compositional matrix adjust. Identities = 220/528 (41%), Positives = 328/528 (62%), Gaps = 23/528 (4%) Query: 6 KLSIGARLYGVSGLLIAALSALAITSWVQLSQVNQLAHRVGEVRVTQLALIASTELNLTR 65 KLSI RLY + G +I LA + V++++ A E V L +++ + Sbjct: 2 KLSIVNRLYILIGAVIIGCVVLAGANMVKMNRTYADASFASENVVPSLLSLSTITQGING 61 Query: 66 VMVDLRQALLVKDAAQIRAALADVDAMRKQITDNDNAYLKDVPTQEGRDAFDRNWLKLQQ 125 + + + L + ++ A + ++ +K++ D L+ ++ DA D+ L+ + Sbjct: 62 MRIATWRYLALTESVDRGAIESSFNSAKKRVED----ALQKYEQEQLADATDKELLRAVR 117 Query: 126 VTW-----PVAEANMALIRDGRTDDAMTMLEQKTAPAFAPLQKWLSEERA------RQGS 174 V++ +AEA + L G+ ++A +L P L K ++E RA + G+ Sbjct: 118 VSYQEFDKSLAEA-LKLANAGQWNEARALLANNQ-PVIDRLNKAMTELRAYNEKISQAGA 175 Query: 175 SVGQEVLDIGNSANLMRMQLTSLVVVIAIGLSLFSWYIGRVLRSRVVQSQQVAQRVKDGD 234 QE + +A L + +++ V+ + ++L I R + + ++ ++A+ + +GD Sbjct: 176 HRAQETMA---AARLHAILISAAVIAV---VTLMGLLIARKIARSLAEAVELAETIAEGD 229 Query: 235 FTVEVRDDARDEFSPLLHTLGEMQTSLTGVVRSVRGNAEYVATASAEISKGNNDLAQRTE 294 T E+ + DE L+ + +M +L +V +VR VA AS++I+ GN DL+ RTE Sbjct: 230 LTREIDTSSTDEIGHLMAAMAKMNRNLVTIVATVRTGVNTVAAASSQIATGNMDLSARTE 289 Query: 295 SQASALEETAASMEELSTTVTQNVDSAREADSLTKAASDIARKGGEVVSEVVQTMRNINE 354 QAS+LE+TAA+ EEL++TV QN ++A +AD L AAS IA +GGEVV +VV TM I Sbjct: 290 QQASSLEQTAAATEELTSTVKQNAENALQADQLASAASGIAAEGGEVVKQVVTTMEEIRS 349 Query: 355 SSKHIADIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAGRSAEAAKQ 414 SS +IADII VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVA+EVRSLA RSA AAK+ Sbjct: 350 SSLNIADIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVATEVRSLAQRSASAAKE 409 Query: 415 IKGLITVSVERIEHGTQLVDQAGQTMDEVVASVARVTDIMSRINEASVEQSAGVGQVSEA 474 IK LI SV ++E+G+QLV AG TM +VV SV RVTD+++ I+ AS EQS G+ QV++A Sbjct: 410 IKQLIDDSVSKVENGSQLVMSAGNTMQQVVESVRRVTDVVTEISAASREQSLGIEQVNQA 469 Query: 475 VTQMDQVTQQNAALVEQTAAAAESLKGQAQQLVNAMSVFKLAQERLTL 522 ++ MDQ TQ+NAALVE+ AAA+SL+ QA+QL A++VFKL QE + + Sbjct: 470 ISHMDQSTQENAALVEEATAAAQSLQEQARQLERAVNVFKLRQEAMDI 517 >gi|300310695|ref|YP_003774787.1| methyl-accepting chemotaxis sensory transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 585 Score = 325 bits (832), Expect = 8e-90, Method: Compositional matrix adjust. Identities = 241/538 (44%), Positives = 324/538 (60%), Gaps = 46/538 (8%) Query: 2 LGITKLSIGARLYGVSGLLIAALSAL---AITSWVQLSQVNQLAHRVGEVRVTQLALIAS 58 + + L IG RLY GL+ A L L A T++ +L N+L EVR QL +A Sbjct: 1 MKLADLKIGTRLYMGFGLVAAILVILVSVAYTNFARLGAANELNVHTYEVRA-QLRAMAE 59 Query: 59 TELNLTRVMVDLRQALLVKDAAQIRAALADVDA---------------MRKQITDNDNAY 103 + +N+ + Q +L+ +DA + K T + Sbjct: 60 SLINI--------------ETGQRGFSLSGIDASLEPYHLGMTTFKTALEKTRTLTSDNT 105 Query: 104 LKDVPTQEGRDAFDRNWLKLQQVTWPVAEANMALIRDGRTD-DAMTMLEQ--KTAPAFAP 160 L+ QE D+ R WLK P E A +R+GR A+ EQ K Sbjct: 106 LQQQRLQELSDSEQR-WLK--SAIDPAIELRRA-VREGREQMAAVVAFEQAGKGKSQMDS 161 Query: 161 LQKWLSEERARQGSSVGQEVLDIGNSANLMRMQLTSLVVVIAIGLSL---FSWYIGRVLR 217 ++ L + + + +G+ + +A L + T+L+ AI L L +W++ + Sbjct: 162 MRVLLDDLDRTESALLGERAEE---AAALRSVTSTTLIGGGAIALMLAVLLAWWLANNIT 218 Query: 218 SRVVQSQQVAQRVKDGDFTVEVRDDARDEFSPLLHTLGEMQTSLTGVVRSVRGNAEYVAT 277 + + Q+A+RV GD T + +RDE LL L +M SL +V VR E +AT Sbjct: 219 RPLALAVQLARRVASGDLTARIEVRSRDETGALLGALKDMNESLVRIVSGVRRGTEMIAT 278 Query: 278 ASAEISKGNNDLAQRTESQASALEETAASMEELSTTVTQNVDSAREADSLTKAASDIARK 337 AS+EI+ GN DL+ RTE QAS+LEETA+SMEEL++TV QN +AREA+ + +AASDIA Sbjct: 279 ASSEIATGNLDLSSRTEQQASSLEETASSMEELTSTVQQNTANAREANRMAQAASDIASS 338 Query: 338 GGEVVSEVVQTMRNINESSKHIADIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVV 397 GG VV+ VV TM IN+SS+ I DII VIDGIAFQTNILALNAAVEAARAGEQGRGFAVV Sbjct: 339 GGGVVARVVDTMEEINDSSRKIVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVV 398 Query: 398 ASEVRSLAGRSAEAAKQIKGLITVSVERIEHGTQLVDQAGQTMDEVVASVARVTDIMSRI 457 ASEVRSLA RSA AAK+IK LI+ SV ++E G+ LV++AG TM E+V SV RVT++++ I Sbjct: 399 ASEVRSLAQRSAAAAKEIKALISDSVAKVESGSLLVNEAGDTMREIVESVQRVTNMINEI 458 Query: 458 NEASVEQSAGVGQVSEAVTQMDQVTQQNAALVEQTAAAAESLKGQAQQLVNAMSVFKL 515 + A EQ +G+ QVS+AV QMDQVTQQNAALVEQ AAA++SL+ QAQ+L A+SVF+L Sbjct: 459 SAAGEEQRSGIEQVSQAVGQMDQVTQQNAALVEQAAAASQSLQEQAQELAQAVSVFRL 516 >gi|300312840|ref|YP_003776932.1| methyl-accepting chemotaxis I protein [Herbaspirillum seropedicae SmR1] Length = 646 Score = 319 bits (818), Expect = 3e-88, Method: Compositional matrix adjust. Identities = 185/311 (59%), Positives = 239/311 (76%) Query: 212 IGRVLRSRVVQSQQVAQRVKDGDFTVEVRDDARDEFSPLLHTLGEMQTSLTGVVRSVRGN 271 I R S + + ++ + + GD T V+ + DE + LL + M L+ VV SVR N Sbjct: 336 ITRATTSAINDAVRLTEAISAGDLTQHVQINGSDEVARLLTAMQAMTARLSTVVSSVRSN 395 Query: 272 AEYVATASAEISKGNNDLAQRTESQASALEETAASMEELSTTVTQNVDSAREADSLTKAA 331 +E VATASA+I++GN DL++RTE QASAL++TAASMEEL +TV QN D AR+A L + A Sbjct: 396 SESVATASAQIAQGNADLSKRTEEQASALQQTAASMEELGSTVAQNADGARQAGDLAREA 455 Query: 332 SDIARKGGEVVSEVVQTMRNINESSKHIADIIGVIDGIAFQTNILALNAAVEAARAGEQG 391 + +A +GG+VV +VVQTM+ ++ SS I+DIIGVIDGIAFQTNILALNAAVEAARAGEQG Sbjct: 456 AHVAGQGGQVVQQVVQTMQGLHASSDRISDIIGVIDGIAFQTNILALNAAVEAARAGEQG 515 Query: 392 RGFAVVASEVRSLAGRSAEAAKQIKGLITVSVERIEHGTQLVDQAGQTMDEVVASVARVT 451 RGFAVVA+EVRSLA RSA AA++IK LIT SVE++ GTQL DQAG+TM+E+V S+ RV+ Sbjct: 516 RGFAVVATEVRSLAQRSATAAREIKELITASVEQVGQGTQLADQAGKTMEEIVTSIRRVS 575 Query: 452 DIMSRINEASVEQSAGVGQVSEAVTQMDQVTQQNAALVEQTAAAAESLKGQAQQLVNAMS 511 DIM IN A+ EQ+ G+ QV +AVTQMD TQQNAALVE++AAAA SL GQA++LV A++ Sbjct: 576 DIMVGINAATAEQNTGIAQVGQAVTQMDTTTQQNAALVEESAAAAISLSGQARELVQAVA 635 Query: 512 VFKLAQERLTL 522 VF+L Q +T+ Sbjct: 636 VFRLGQRAVTV 646 >gi|300313897|ref|YP_003777989.1| methyl-accepting chemotaxis protein I [Herbaspirillum seropedicae SmR1] Length = 516 Score = 316 bits (810), Expect = 3e-87, Method: Compositional matrix adjust. Identities = 193/330 (58%), Positives = 247/330 (74%), Gaps = 3/330 (0%) Query: 186 SANLMRMQLTSLVVVIAIGLSLFSWYIGRVLRSRVVQSQQVAQRVKDGDFTVEVRDDARD 245 SA L + L + +VVIA+GL++ + R + + ++ VA+RV GD T+++R + D Sbjct: 188 SATLWIIGLLAAIVVIALGLAVM---VARAVSRPLNEAVAVAERVAQGDLTMDIRATSTD 244 Query: 246 EFSPLLHTLGEMQTSLTGVVRSVRGNAEYVATASAEISKGNNDLAQRTESQASALEETAA 305 E L+H+L M SL +V VR + + TAS EI+ GN DL+ RTE QA +LEETA+ Sbjct: 245 ETGMLMHSLRAMNDSLQQIVGEVRTGTDTITTASQEIATGNLDLSSRTEEQAGSLEETAS 304 Query: 306 SMEELSTTVTQNVDSAREADSLTKAASDIARKGGEVVSEVVQTMRNINESSKHIADIIGV 365 +MEEL+ TV QN D+AR+A+ L +ASDIA +GG VV +VVQTM I ESS IADII V Sbjct: 305 AMEELTATVKQNADNARQANQLAASASDIATQGGAVVGQVVQTMEGITESSNRIADIISV 364 Query: 366 IDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAGRSAEAAKQIKGLITVSVER 425 IDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLA RSA AAK+IK LI SVE+ Sbjct: 365 IDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAKEIKQLIDDSVEK 424 Query: 426 IEHGTQLVDQAGQTMDEVVASVARVTDIMSRINEASVEQSAGVGQVSEAVTQMDQVTQQN 485 + G+QLV++AG TM EVVASV RVTD+++ I+ AS EQS G+ +++ A+TQMD+VTQQN Sbjct: 425 VGAGSQLVERAGATMSEVVASVRRVTDVVAEISAASNEQSTGIEEINRAITQMDEVTQQN 484 Query: 486 AALVEQTAAAAESLKGQAQQLVNAMSVFKL 515 AALVE+ AAAA+SL+ QA +L +SVFKL Sbjct: 485 AALVEEAAAAAQSLQDQAARLSQVVSVFKL 514 >gi|300313820|ref|YP_003777912.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 597 Score = 315 bits (808), Expect = 4e-87, Method: Compositional matrix adjust. Identities = 205/417 (49%), Positives = 279/417 (66%), Gaps = 31/417 (7%) Query: 110 QEGRDAFDRNWLKLQQVTWPVAEANMALIRDGRTDDAMTMLEQKTAPA----------FA 159 QE R A++ KL++ LI+ + ++A+ +L + PA F Sbjct: 119 QEARSAYNTPRDKLRE-----------LIKQQKKEEAVELLFTQVIPAQDKYFQVLDQFV 167 Query: 160 PLQKWLSEERARQGSSVGQEVLDIGNSANLMRMQLTSLVVVIAIGLSLFSWYIGRVLRSR 219 QK L E+ +G D SA M + L+++ + + + + +W + R + Sbjct: 168 AFQKSLMEQSVTEGR-------DTTQSAIAMMLGLSAVAIALCV---IAAWLVTRSITRP 217 Query: 220 VVQSQQVAQRVKDGDFTVEVRDDARDEFSPLLHTLGEMQTSLTGVVRSVRGNAEYVATAS 279 + ++ VA V GD TV++ ++DE LL L M SL +V VR ++ + TAS Sbjct: 218 LNEAVSVASAVAAGDLTVQIGATSKDETGALLAALKSMNDSLHRIVSEVRQGSDTINTAS 277 Query: 280 AEISKGNNDLAQRTESQASALEETAASMEELSTTVTQNVDSAREADSLTKAASDIARKGG 339 AEI+ GN DL+ RTE QA ALEETA++MEEL++TV QN D+AR+A+SL AS++A +GG Sbjct: 278 AEIATGNLDLSSRTEEQAGALEETASAMEELTSTVKQNADNARQANSLAATASEVAVQGG 337 Query: 340 EVVSEVVQTMRNINESSKHIADIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAS 399 VV +VVQTM IN++S+ I DII VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAS Sbjct: 338 SVVGQVVQTMGEINDASRKIVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAS 397 Query: 400 EVRSLAGRSAEAAKQIKGLITVSVERIEHGTQLVDQAGQTMDEVVASVARVTDIMSRINE 459 EVR+LA RSA AAK+IK LI SV R+++G++LV+QAG TM EVVASV RVTD+++ I+ Sbjct: 398 EVRTLAQRSASAAKEIKALIDDSVSRVDNGSRLVEQAGTTMTEVVASVRRVTDVVAEISA 457 Query: 460 ASVEQSAGVGQVSEAVTQMDQVTQQNAALVEQTAAAAESLKGQAQQLVNAMSVFKLA 516 AS EQS G+ Q++ A+TQMDQVTQQNAALVE+ AAAA+SL+ Q+ +L +SVFKL+ Sbjct: 458 ASNEQSDGIEQINHAITQMDQVTQQNAALVEEAAAAAQSLQEQSTRLSATVSVFKLS 514 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.126 0.328 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,145,077 Number of extensions: 38842 Number of successful extensions: 572 Number of sequences better than 1.0e-08: 70 Number of HSP's gapped: 303 Number of HSP's successfully gapped: 72 Length of query: 526 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 436 Effective length of database: 1,193,720 Effective search space: 520461920 Effective search space used: 520461920 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)