BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1239.p (486 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310755|ref|YP_003774847.1| Zn-dependent protease [Herbaspi... 916 0.0 gi|300312123|ref|YP_003776215.1| Zn-dependent protease TldD prot... 492 e-140 gi|300311183|ref|YP_003775275.1| Zn-dependent protease [Herbaspi... 76 7e-15 gi|300312124|ref|YP_003776216.1| Zn-dependent protease TLDE prot... 65 9e-12 >gi|300310755|ref|YP_003774847.1| Zn-dependent protease [Herbaspirillum seropedicae SmR1] Length = 486 Score = 916 bits (2367), Expect = 0.0, Method: Compositional matrix adjust. Identities = 475/486 (97%), Positives = 479/486 (98%) Query: 1 MKLFEPNMGALQVARDVLLTPFGLDESKLLKALGSMFTHKVDYADLYFQFTKSEGWSLEE 60 MKLFEPNMGALQVARDVLLTPFGLDESKLLKALGSMFTHKVDYADLYFQFTKSEGWSLEE Sbjct: 1 MKLFEPNMGALQVARDVLLTPFGLDESKLLKALGSMFTHKVDYADLYFQFTKSEGWSLEE 60 Query: 61 GIVKTGSFSIDQGVGVRAVSGDKTAFSYSDEISESALMDAAAATRTIARQGSGKIKVAGA 120 GIVKTGSFSIDQGVGVRAVSGDKTAFSYSDEISESALMDAAAATRTIARQGSGKIKVAGA Sbjct: 61 GIVKTGSFSIDQGVGVRAVSGDKTAFSYSDEISESALMDAAAATRTIARQGSGKIKVAGA 120 Query: 121 MTLAGGRALYLPHDPLTSLDATAKVQLLERVERIARAKDPRVKQVMASLSGEYDVVLVAR 180 M GGRALYLPHDPLTSLDATAKVQLLERVERIARAKDPRVKQVMASLSGEYDVVLVAR Sbjct: 121 MIPGGGRALYLPHDPLTSLDATAKVQLLERVERIARAKDPRVKQVMASLSGEYDVVLVAR 180 Query: 181 ADGVLAADIRPLVRVSVTVIAEQNGRREMGSSGGGGRYDYAYFSDTLLEKYASEAVATAL 240 ADGVLAADIRPLVRVSVTVIAEQNGRREMGSSGGGGRYDY YFSD LLE+YASEAVATAL Sbjct: 181 ADGVLAADIRPLVRVSVTVIAEQNGRREMGSSGGGGRYDYGYFSDVLLEQYASEAVATAL 240 Query: 241 VNLDARPAPAGPMTVVLGPGWPGILLHEAIGHGLEGDFNRKGSSTFSGRIGERVAAKGVT 300 VNL++RPAPAGPMTVVLGPGWPGILLHEAIGHGLEGDFNRKGSSTFSGRIGERVAAKGVT Sbjct: 241 VNLESRPAPAGPMTVVLGPGWPGILLHEAIGHGLEGDFNRKGSSTFSGRIGERVAAKGVT 300 Query: 301 VVDDGTIADRRGSLNIDDEGNPTQCTTLIEDGILKGYIQDTMNARLMKMDVTGNARRESF 360 VVDDGTIADRRGSLNIDDEGNPTQCTTLIEDGILKGYIQDTMNARLMKMDVTGNARRESF Sbjct: 301 VVDDGTIADRRGSLNIDDEGNPTQCTTLIEDGILKGYIQDTMNARLMKMDVTGNARRESF 360 Query: 361 AHLPMPRMTNTYMLGGDKDPGEILASVKNGLYAVNFGGGQVDITNGKFVFSASEAYMIED 420 AHLPMPRMTNTYMLGGD+DP EILASVKNGLYAVNFGGGQVDITNGKFVFSASEAYMIED Sbjct: 361 AHLPMPRMTNTYMLGGDRDPAEILASVKNGLYAVNFGGGQVDITNGKFVFSASEAYMIED 420 Query: 421 GKITYPVKGATLIGNGPDVLNRVSMIGNDMRLDPGVGVCGKEGQSVPVGVGQPTLRLDGV 480 GKITYPVKGATLIGNGPDVLNRVSMIGNDMRLD GVGVCGKEGQSVPVGVGQPTLRLDGV Sbjct: 421 GKITYPVKGATLIGNGPDVLNRVSMIGNDMRLDSGVGVCGKEGQSVPVGVGQPTLRLDGV 480 Query: 481 TVGGTA 486 TVGGTA Sbjct: 481 TVGGTA 486 >gi|300312123|ref|YP_003776215.1| Zn-dependent protease TldD protein [Herbaspirillum seropedicae SmR1] Length = 501 Score = 492 bits (1266), Expect = e-140, Method: Compositional matrix adjust. Identities = 265/494 (53%), Positives = 332/494 (67%), Gaps = 16/494 (3%) Query: 5 EPNMG--------ALQVARDVLLTPFGLDESKLLKALGSMFTHKVDYADLYFQFTKSEGW 56 EPN G A AR LL GLDES+LL + DYADLY + + +E W Sbjct: 12 EPNDGGADSGTSTAYCRARWGLLAASGLDESQLLAGVSRALRKGGDYADLYLKSSVAESW 71 Query: 57 SLEEGIVKTGSFSIDQGVGVRAVSGDKTAFSYSDEISESALMDAAAATRTIARQGSGKIK 116 LE G V +++ QG G+R V G+++ + +D + + AA +A G Sbjct: 72 HLERGKVTRSNYNQSQGFGLRLVEGEQSVLTSADRFDATTIGKAA----DLAADAVGHAP 127 Query: 117 VAGAMTLAG----GRALYLPHDPLTSLDATAKVQLLERVERIARAKDPRVKQVMASLSGE 172 V+ A + G Y DPL SLDA K+ LLE V+R RA+ P V +VMASLS Sbjct: 128 VSPAGLVLKLPKRGPTYYTLDDPLPSLDAAHKIALLESVDRACRARSPLVVEVMASLSIS 187 Query: 173 YDVVLVARADGVLAADIRPLVRVSVTVIAEQNGRREMGSSGGGGRYDYAYFSDTLLEKYA 232 D+V +AR DG D+RPLVR+ V V +NGRRE S GGR A F +E A Sbjct: 188 LDLVFLARNDGYCGGDVRPLVRLDVAVQVVRNGRREGASRTLGGRVGLATFDHAAIESVA 247 Query: 233 SEAVATALVNLDARPAPAGPMTVVLGPGWPGILLHEAIGHGLEGDFNRKGSSTFSGRIGE 292 + AV AL+ L+AR APAG MTVV+GPGW G++LHEA+GHGLEGDFNR+GSS F+GRIG+ Sbjct: 248 ALAVDAALIKLEARAAPAGTMTVVIGPGWNGVMLHEAVGHGLEGDFNRRGSSAFAGRIGQ 307 Query: 293 RVAAKGVTVVDDGTIADRRGSLNIDDEGNPTQCTTLIEDGILKGYIQDTMNARLMKMDVT 352 RVAA GVT+VDDGT+ RGSLNIDDEG P QCTTLIEDGIL Y+QD++NARLM + T Sbjct: 308 RVAAPGVTIVDDGTVPGSRGSLNIDDEGTPGQCTTLIEDGILVSYMQDSLNARLMGVANT 367 Query: 353 GNARRESFAHLPMPRMTNTYMLGGDKDPGEILASVKNGLYAVNFGGGQVDITNGKFVFSA 412 GN RRES+ LPMPRMTNTYM G DP EI+ASVK G+Y + GGQVDI++G+F+F+A Sbjct: 368 GNGRRESYDVLPMPRMTNTYMRAGGVDPAEIIASVKQGIYIAHLEGGQVDISSGRFMFAA 427 Query: 413 SEAYMIEDGKITYPVKGATLIGNGPDVLNRVSMIGNDMRLDPGVGVCGKEGQSVPVGVGQ 472 SEA++IE+G++ PVKGAT++GNGP L V +IGND+ ++ GVCGK GQSVPVGVGQ Sbjct: 428 SEAFLIENGRLGAPVKGATVLGNGPQSLKNVKLIGNDLEINTASGVCGKAGQSVPVGVGQ 487 Query: 473 PTLRLDGVTVGGTA 486 PTLR+DG+TVGGTA Sbjct: 488 PTLRIDGMTVGGTA 501 >gi|300311183|ref|YP_003775275.1| Zn-dependent protease [Herbaspirillum seropedicae SmR1] Length = 454 Score = 75.9 bits (185), Expect = 7e-15, Method: Compositional matrix adjust. Identities = 109/451 (24%), Positives = 184/451 (40%), Gaps = 43/451 (9%) Query: 20 TPFGLDESKLLKALGSMFTHKVDY--ADLYFQFTKSEGWSL--EEGIVKTGSFSIDQGVG 75 TPF + +L + + + D +D ++ G S+ +G ++T + D+G+G Sbjct: 4 TPFTHSQDQLKQLAQDVLRYARDKGASDAAVDISEGSGLSVAVRKGKIETIEQNKDKGIG 63 Query: 76 VRAVSGD----KTAFSYSDEISESALMDAAAATRTIARQGSGKIKVAGAMTLAGGRALYL 131 V G+ + + + + S+ AL + A IAR S + L L + Sbjct: 64 VTVFLGEGRQVRRGNASTSDFSQQALKETVEAAYNIARFTS----IDDCAGLPDAEMLEM 119 Query: 132 -PHD-----PLTSLDATAKVQLLERVERIARAKDPRVKQVM-ASLSGEYDVVLVARADGV 184 P D P ++ A V L +R E A DPR+ A + ++ + A + G Sbjct: 120 APRDLKLFSPW-NISAEEAVALAQRCETAALETDPRISNSEGAGVYVQHSHFIAANSRGF 178 Query: 185 LAADIRPLVR--VSVTVIAEQNGRREMGSSGGGGRYDYAYFSDTLLEKYASEAVATALVN 242 + P R +SV IA + + R + +YA+E AL Sbjct: 179 MGG--YPFSRHTISVAPIAGKGASMQRDDWYSSKRDPKKLAQPEAIGRYAAE---RALAR 233 Query: 243 LDARPAPAGPMTVV----LGPGWPGILLHEAIGHGLEGDFNRKGSSTFSGRIGERVAAKG 298 L+AR V+ L G G + G G RK S+ +G++V + Sbjct: 234 LNARKLSTRKCPVLFEAPLAAGLLGAFVQAVSG----GALYRK-STFLLDSLGQQVFPEH 288 Query: 299 VTVVDDGTIADRRGSLNIDDEGNPTQCTTLIEDGILKGYIQDTMNARLMKMDVTGNARRE 358 + +++D + GS D+EG T ++ +G+++GY T +AR + M TGN+ Sbjct: 289 IQILEDPHVIGGVGSAPFDEEGVRTLKREVVRNGVVQGYFLSTYSARKLGMQTTGNSGGS 348 Query: 359 SFAHLPMPRMTNTYMLGGDKDPGEILASVKNGLYAVNFGGGQVDITNGKFVFSASEAYMI 418 L T G +L + GL G V+ G + AS Y + Sbjct: 349 HNLTLRSELTTRADTFKG------MLKKMGTGLLVTELMGQGVNYVTGDYSRGAS-GYWV 401 Query: 419 EDGKITYPVKGATLIGNGPDVLNRVSMIGND 449 E+G I YPV+ T+ GN D+L ++ +GND Sbjct: 402 ENGVIQYPVEEITIAGNMKDMLRQIVAVGND 432 >gi|300312124|ref|YP_003776216.1| Zn-dependent protease TLDE protein [Herbaspirillum seropedicae SmR1] Length = 457 Score = 65.5 bits (158), Expect = 9e-12, Method: Compositional matrix adjust. Identities = 77/306 (25%), Positives = 125/306 (40%), Gaps = 12/306 (3%) Query: 145 VQLLERVERIARAKDPRVKQV-MASLSGEYDVVLVARADGVLAADIRPLVRVSVTVIAEQ 203 V L+ R+ER A A V Q+ A L ++A ++G L + +S T +A + Sbjct: 141 VALVARIERAALAVSSAVPQIEQAVLFSSRTRRVLANSEGFLRSSASTRHSLSCTAVAIR 200 Query: 204 NGRREMGSSGGGGRYDYAYFSDTLLEKYASEAVATALVNLDARPAPAGPMTVVLGPGWPG 263 +G +E+ S R D A E + A AL L AR V+ P Sbjct: 201 DGAKEI-ESWWDARRDPAQLPSP--ESLGACAGRRALARLGARQVATQTCPVLFEPQAAA 257 Query: 264 ILLHEAIGHGLEGDFNRKGSSTFSGRIGERVAAKGVTVVDDGTIADRRGSLNIDDEGNPT 323 LL+E + + SS +G+ V A + + DD + GS + D G Sbjct: 258 SLLNEFV-QAISARPLYMHSSFLRDALGQEVFAAHMNISDDPLLKGGMGSRSYDGNGAAL 316 Query: 324 QCTTLIEDGILKGYIQDTMNARLMKMDVTGNARRESFAHLPMPRMTNTYMLGGDKDPGEI 383 + TLI G L+GY AR + + + + P M ++ D + Sbjct: 317 RPRTLIAGGRLQGYYLGAYGARRLGLPLAEHGAG------PSNVMVSSTTSAASGDLEAM 370 Query: 384 LASVKNGLYAVNFGGGQVDITNGKFVFSASEAYMIEDGKITYPVKGATLIGNGPDVLNRV 443 + + GL G V++ G F + S + +E+G+I YPV G T+ N ++ + Sbjct: 371 IRRMGTGLVISGLNGQGVNLMTGDFSRACS-GFWVENGQIAYPVAGITVASNLREMFAGI 429 Query: 444 SMIGND 449 +GND Sbjct: 430 VAVGND 435 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.136 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,560,709 Number of extensions: 69648 Number of successful extensions: 237 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 234 Number of HSP's successfully gapped: 4 Length of query: 486 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 397 Effective length of database: 1,198,455 Effective search space: 475786635 Effective search space used: 475786635 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)