BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1231.p (664 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310747|ref|YP_003774839.1| transketolase [Herbaspirillum s... 1355 0.0 gi|300309841|ref|YP_003773933.1| transketolase [Herbaspirillum s... 555 e-159 gi|300309645|ref|YP_003773737.1| transketolase N-terminal subuni... 115 1e-26 gi|300310439|ref|YP_003774531.1| transketolase alpha subunit [He... 100 4e-22 gi|300309646|ref|YP_003773738.1| transketolase C-terminal subuni... 76 8e-15 >gi|300310747|ref|YP_003774839.1| transketolase [Herbaspirillum seropedicae SmR1] Length = 664 Score = 1355 bits (3508), Expect = 0.0, Method: Compositional matrix adjust. Identities = 653/664 (98%), Positives = 662/664 (99%) Query: 1 MISTLPTDKMANAIRALAMDAVQKANSGHPGMPMGMAEIAVALWAKHYRHNPSNPHWANR 60 MISTLPTDKMANAIRALAMDAVQKANSGHPGMPMGMAEIAVALWAKHYRHNP+NPHWANR Sbjct: 1 MISTLPTDKMANAIRALAMDAVQKANSGHPGMPMGMAEIAVALWAKHYRHNPANPHWANR 60 Query: 61 DRFVLSNGHGSMLQYALLHLTGYDLSMEDIKSFRQLHSKTAGHPEVHITPGVETTTGPLG 120 DRFVLSNGHGSMLQYALLHLTGYDLSMEDIKSFRQLHSKTAGHPEVHITPGVETTTGPLG Sbjct: 61 DRFVLSNGHGSMLQYALLHLTGYDLSMEDIKSFRQLHSKTAGHPEVHITPGVETTTGPLG 120 Query: 121 QGITNAVGMALAEKLLAAEFNKPGLAVVDHYTYAFVGDGCLMEGISHEACSLAGVLNLSK 180 QGITNAVGMALAEKLLAAEFNKPGLAVVDHYTYAFVGDGCLMEGISHEACSLAGVLNLSK Sbjct: 121 QGITNAVGMALAEKLLAAEFNKPGLAVVDHYTYAFVGDGCLMEGISHEACSLAGVLNLSK 180 Query: 181 LIVLYDDNGISIDGHVEGWFKDDTPKRFEAYGWNVIPAVDGHNVEAVSAAIHQAKLADKP 240 LIVLYDDNGISIDGHVEGWFKDDTPKRFEAYGWNVIPAVDGH+VEAVSAAIHQAKLADKP Sbjct: 181 LIVLYDDNGISIDGHVEGWFKDDTPKRFEAYGWNVIPAVDGHSVEAVSAAIHQAKLADKP 240 Query: 241 TLICCRTVIGKGSPNLAGTDKVHGAALGDKEIAAVREALGWTAAPFEIPADVYAAWDAKA 300 TLICCRTVIGKGSPNLAGTDKVHGAALGDKEIAAVREALGWT+APFEIPADVY+AWDAKA Sbjct: 241 TLICCRTVIGKGSPNLAGTDKVHGAALGDKEIAAVREALGWTSAPFEIPADVYSAWDAKA 300 Query: 301 TGADFEASWKQTFDTYAAQYPAEAAELLRRLKGELPVAFESTVTAYINSTLEKKETIATR 360 TGADFEASWKQTFDTYAAQYP+EAAELLRRLKGELP AFESTVTAYINSTLEKKETIATR Sbjct: 301 TGADFEASWKQTFDTYAAQYPSEAAELLRRLKGELPAAFESTVTAYINSTLEKKETIATR 360 Query: 361 KASQNAIQAYAQALPEFLGGSADLTGSNLTNWKESVAVRADQRGNHINYGVREFGMSAIM 420 KASQNAIQAYAQALPEFLGGSADLTGSNLTNWKESVAVRADQRGNHINYGVREFGMSAIM Sbjct: 361 KASQNAIQAYAQALPEFLGGSADLTGSNLTNWKESVAVRADQRGNHINYGVREFGMSAIM 420 Query: 421 NGIALHGGYLPFGATFLTFSDYSRNALRMAALMKIRSLFVFTHDSIGLGEDGPTHQSVEH 480 NGIALHGGYLPFGATFLTFSDYSRNALRMAALMKIR+LFVFTHDSIGLGEDGPTHQSVEH Sbjct: 421 NGIALHGGYLPFGATFLTFSDYSRNALRMAALMKIRALFVFTHDSIGLGEDGPTHQSVEH 480 Query: 481 VSSLRLIPNLDNWRPCDTTETAVAWAESIKRHDGPSTLIFSRQNLPFQERTDAQVKDIAR 540 VSSLRLIPNLDNWRPCDTTETAVAWAESIKRHDGPSTLIFSRQNLPFQERTDAQVKDIAR Sbjct: 481 VSSLRLIPNLDNWRPCDTTETAVAWAESIKRHDGPSTLIFSRQNLPFQERTDAQVKDIAR 540 Query: 541 GGYVLRDAPNAKVVLMATGSEIELATKAADALTAEGIAVRVVSMPSTDVFDRQDSAYKAT 600 GGYVLRDAPNAKV+LMATGSEIELATKAADALTAEGIAVRVVSMPSTDVFDRQD+AYKA+ Sbjct: 541 GGYVLRDAPNAKVILMATGSEIELATKAADALTAEGIAVRVVSMPSTDVFDRQDAAYKAS 600 Query: 601 VLPRGVPRVAIEAGVTAFWHKYVGLEGAVVGIDTFGESAPAGVLFKHFGFTVENVVAKAK 660 VL RGVPRVAIEAGVTAFWHKYVGLEGAVVGIDTFGESAPAGVLFKHFGFTVENVVAKAK Sbjct: 601 VLTRGVPRVAIEAGVTAFWHKYVGLEGAVVGIDTFGESAPAGVLFKHFGFTVENVVAKAK 660 Query: 661 AVLA 664 AVLA Sbjct: 661 AVLA 664 >gi|300309841|ref|YP_003773933.1| transketolase [Herbaspirillum seropedicae SmR1] Length = 664 Score = 555 bits (1431), Expect = e-159, Method: Compositional matrix adjust. Identities = 296/661 (44%), Positives = 407/661 (61%), Gaps = 9/661 (1%) Query: 6 PTDKMANAIRALAMDAVQKANSGHPGMPMGMAEIAVALWAKHYRHNPSNPHWANRDRFVL 65 P +A+AIR L++DA+ +A GH G+P+GMAEIA AL+A+H R NP+ P W +RDR VL Sbjct: 5 PLQPLAHAIRFLSIDAIVRATEGHQGVPLGMAEIATALYARHLRFNPAAPTWWDRDRVVL 64 Query: 66 SNGHGSMLQYALLHLTGYD-LSMEDIKSFRQLHSKTAGHPEVHITPGVETTTGPLGQGIT 124 SNGHGSML Y LL+LTGY+ +++E ++SFR+ S AGHPE G+E TTGPLGQGI Sbjct: 65 SNGHGSMLLYCLLYLTGYEHITLEQVRSFREFGSHCAGHPEYAPELGIEVTTGPLGQGIA 124 Query: 125 NAVGMALAEKLLAAEFNKPGLAVVDHYTYAFVGDGCLMEGISHEACSLAGVLNLSKLIVL 184 NA GMA+AE L A F G +V+H TYAFVGDGCL EGI E SLAG L L KL +L Sbjct: 125 NAFGMAVAEAYLNARF---GDDIVNHRTYAFVGDGCLQEGIGQEMISLAGHLRLHKLTLL 181 Query: 185 YDDNGISIDGHVEGWFKDDTPKRFEAYGWNVIPAVDGHNVEAVSAAIHQAKLADKPTLIC 244 +DDN I+ DG E ++ +RF GW+V+ VDGH+++AVS A+ A+ +D+P++I Sbjct: 182 WDDNQITDDGKTELSISENVAERFRVAGWHVV-EVDGHDIQAVSRALDAAQGSDRPSMIA 240 Query: 245 CRTVIGKGSPNLAGTDKVHGAALGDKEIAAVREALGWTAAPFEIPADVYAAWDAKATGAD 304 CRTVIG+G P + G H A L + + A RE L W APF++P D+ A W ++ G Sbjct: 241 CRTVIGRGIPRVQGQRGGHSARLYEDDAQAARELLQWPHAPFDVPQDILAQW--RSFGQR 298 Query: 305 FEASWKQTFDTYAAQYPAEAAELLRRLKGELPVAFESTVTAYINSTLEKKETIATRKASQ 364 +A +Q A A+ AE R ++GELP ++ + + S E ++ S Sbjct: 299 NDAQHQQWQARVQALPAAQRAEFERVMRGELPSGWQEVLLPFKRSAHEPEQAQGGILISA 358 Query: 365 NAIQAYAQALPEFLGGSADLTGSNLTNWKESVAVRADQRGNHINYGVREFGMSAIMNGIA 424 + A+ +PE + G ADL D+ G +++ GVRE M A+ NG+A Sbjct: 359 AINKLLAEVMPERMVGCADLEAPTAHKGGLHAFTADDRSGAYVHCGVREHLMGAMANGMA 418 Query: 425 LHGGYLPFGATFLTFSDYSRNALRMAALMKIRSLFVFTHDSIGLGEDGPTHQSVEHVSSL 484 HGG + T+L FSDY R A+RM+ALM + +VF+HDSIG+G++GPTHQ VE ++SL Sbjct: 419 AHGGVIALSVTYLAFSDYERPAMRMSALMGLPVKYVFSHDSIGIGKNGPTHQPVEILASL 478 Query: 485 RLIPNLDNWRPCDTTETAVAWAESIKRHDGPSTLIFSRQNLPFQERTDAQVKDIARGGYV 544 R +PN+ RP D TE W +++ GP +++F+RQ LP R ARGGYV Sbjct: 479 RAMPNMLVMRPADATEAVECWEIAMQHRSGPVSMVFARQALPPVRRQYEAENRCARGGYV 538 Query: 545 LRDAPNA--KVVLMATGSEIELATKAADALTAEGIAVRVVSMPSTDVFDRQDSAYKATVL 602 L +A +V L+ATGSE+ LA A L A GIA VVS+P ++FD+QD+AY+ VL Sbjct: 539 LHEAQCGARQVTLLATGSEVALACAARAQLEAAGIATAVVSLPCWELFDQQDTAYRQQVL 598 Query: 603 PRGVPRVAIEAGVTAFWHKYVGLEGAVVGIDTFGESAPAGVLFKHFGFTVENVVAKAKAV 662 RV +EA + W Y+G G VG+ FG PA VL++HF T E VVA+A+ + Sbjct: 599 GEQTVRVGVEAALRFGWDHYLGARGGFVGMPGFGAPGPADVLYRHFNITAEAVVAEARRL 658 Query: 663 L 663 L Sbjct: 659 L 659 >gi|300309645|ref|YP_003773737.1| transketolase N-terminal subunit [Herbaspirillum seropedicae SmR1] Length = 272 Score = 115 bits (287), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 85/223 (38%), Positives = 113/223 (50%), Gaps = 17/223 (7%) Query: 38 EIAVALW-AKHYRHNPSNPHWANRDRFVLSNGHGSMLQYALLHLTGYDLSMEDIKSFRQL 96 +I VAL+ R NP++P A RDR V+S GHGS+ Y +L GY ME+++ + Sbjct: 39 DIFVALYYGGVLRFNPADPRDAGRDRCVISKGHGSICMYPILADLGY-FPMEELQHVCEA 97 Query: 97 HSKTAGHPEVHITPGVETTTGPLGQGITNAVGMALAEKLLAAEFNKPGLAVVDHYTYAFV 156 S G P+ + PG ET G LG G+ A GMAL K + D + Sbjct: 98 GSFLGGIPDP-VIPGYETVNGSLGHGLGVATGMALGLKRSGS----------DRSVFVVA 146 Query: 157 GDGCLMEGISHEACSLAGVLNLSKLIVLYDDNGISIDGHVEGWFKD-DTPKRFEAYGWNV 215 GDG L EG + EA A L L ++ DDN IS+ G+ E + R A+GW+ Sbjct: 147 GDGELHEGANWEAIMFASQHKLDNLHLIVDDNRISMLGYTEDIVSHGELATRLSAFGWDC 206 Query: 216 IPAVDGHNVEAVSAAIHQAK--LADKPTLICCRTVIGKGSPNL 256 + VDGH+V AV AA+ Q K A KP I RT+ G G P L Sbjct: 207 M-EVDGHDVLAVQAALLQQKAHAAGKPKAIIARTLKGHGVPGL 248 >gi|300310439|ref|YP_003774531.1| transketolase alpha subunit [Herbaspirillum seropedicae SmR1] Length = 281 Score = 100 bits (249), Expect = 4e-22, Method: Compositional matrix adjust. Identities = 85/257 (33%), Positives = 124/257 (48%), Gaps = 24/257 (9%) Query: 12 NAIRALAMDAVQKANSGHPGMPMGMAEIAVALWAKHYRHNPSNPHWANRDRFVLSNGHGS 71 NA+R M VQ G+ G +G A++ + P +P W+ RDRF+LS+GH + Sbjct: 18 NAVR---MGEVQ--GQGYIGQALGWADVLAVAYKHAMSLRPDDPQWSGRDRFLLSHGHYA 72 Query: 72 MLQYALLHLTGYDLSMEDIKSFRQLHSKTAGHPEVHITPGVETTTGPLGQGITNAVGMAL 131 + YA L G + E++ S+ S+ TPG+E + G LGQG+ VGMAL Sbjct: 73 IALYAALIEAGI-VPDEELDSYGSDDSRLPMSGMATYTPGMEISGGSLGQGLAIGVGMAL 131 Query: 132 AEKLLAAEFNKPGLAVVDHYTYAFVGDGCLMEGISHEACSLAGVLNLSKLIVLYDDNGIS 191 + N P + Y + DG L EG + EA A L LI L D N Sbjct: 132 GLRY----NNNPA------FVYNSMSDGELDEGSTWEAAMGAAHHQLGNLICLVDINNQQ 181 Query: 192 ID---GHVEGWFKDDTPKRFEAYGWNVIPAVDGHNVEAVSAAIHQAK-LAD-KPTLICCR 246 D G + G+ + ++ A+GW + VDG+++ AV A A+ L D KP +I Sbjct: 182 ADGPSGKIMGF--EPLADKWAAFGW-YVQRVDGNDLAAVVQAFDNARNLKDAKPRVILFD 238 Query: 247 TVIGKGSPNLAGTDKVH 263 T++GKG P L +K H Sbjct: 239 TLMGKGVPFLEQREKNH 255 >gi|300309646|ref|YP_003773738.1| transketolase C-terminal subunit [Herbaspirillum seropedicae SmR1] Length = 321 Score = 76.3 bits (186), Expect = 8e-15, Method: Compositional matrix adjust. Identities = 66/203 (32%), Positives = 99/203 (48%), Gaps = 23/203 (11%) Query: 398 VRADQRGNHINYGVREFGMSAIMNGIALHGGYLPFG---ATFLTFSDYSRNALRMAALMK 454 +RAD +N G+ E + + G+AL G Y F A F+T Y + + +A L + Sbjct: 41 IRADFPDRFVNVGIAEQNLINVSAGLALEG-YKVFAYAIAPFITMRCYEQVRVSLALLSE 99 Query: 455 IRSLFVFTHDSIGLGE------DGPTHQSVEHVSSLRLIPNLDNWRPCDTTETAVAWAES 508 +R + V + IG+G GPTHQ E ++ +R +PN D TA A+ ++ Sbjct: 100 VRPMNV---NLIGVGAGYSYVVSGPTHQCYEDITLMRALPNFQVLSAADHV-TAAAFFDT 155 Query: 509 IKRHDGPSTLIFSRQNLPFQERTDAQVKDIARGGYVLRDAPNAKVVLMATGSEIELATKA 568 GP L F Q LP A D+A+G ++ R K+ L+ATG + A +A Sbjct: 156 CSNSMGPKYLRFDAQVLPVIYGDGA--PDLAQGFHIHRR--GGKIGLVATGYMLHTALQA 211 Query: 569 ADALTAEGIAVRVVSMPSTDVFD 591 A+ L AEGI V V+ D+FD Sbjct: 212 AEVLAAEGIEVGVI-----DLFD 229 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.132 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,188,158 Number of extensions: 96511 Number of successful extensions: 300 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 286 Number of HSP's successfully gapped: 5 Length of query: 664 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 573 Effective length of database: 1,188,985 Effective search space: 681288405 Effective search space used: 681288405 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)