BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1220.p (227 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310740|ref|YP_003774832.1| isochorismatase family protein ... 434 e-123 gi|300310742|ref|YP_003774834.1| cysteine hydrolase falmily prot... 209 2e-55 gi|300310658|ref|YP_003774750.1| 2,3-dihydro-2,3-dihydroxybenzoa... 67 1e-12 gi|300313872|ref|YP_003777964.1| 2,3-dihydro-2,3-dihydroxybenzoa... 54 9e-09 >gi|300310740|ref|YP_003774832.1| isochorismatase family protein [Herbaspirillum seropedicae SmR1] Length = 227 Score = 434 bits (1115), Expect = e-123, Method: Compositional matrix adjust. Identities = 210/227 (92%), Positives = 215/227 (94%) Query: 1 MIHIDALPYPYQFHPRSTALVVIDMQRDFVEEGGFGSALGNDVRPLGAIVPTVAALLTLA 60 MI IDA PYPYQFHPRSTALVVIDMQRDFVEEGGFGSALGNDVRPLGAIVPTVAALL LA Sbjct: 1 MIRIDAFPYPYQFHPRSTALVVIDMQRDFVEEGGFGSALGNDVRPLGAIVPTVAALLALA 60 Query: 61 RAHQMLVVHTRESHLPDLSDCPRAKRLRGNPTLGIGDVGPMGRILVRGEPGNQILPQLAP 120 R QMLVVHTRESHLPDLSDCPR KRLRGNPTLGIGDVGPMGRILVRGEPGNQILPQL P Sbjct: 61 RQTQMLVVHTRESHLPDLSDCPRTKRLRGNPTLGIGDVGPMGRILVRGEPGNQILPQLTP 120 Query: 121 IEGEIVIDKPGKGAFYATDLHAQLLERGITHLLIAGVTTEVCVQTSMREANDRGYECLVV 180 + GEIVIDKPGKGAFYATDLHAQL ERGITHLL+AGVTTEVCVQTSMREANDRGYECLVV Sbjct: 121 MAGEIVIDKPGKGAFYATDLHAQLQERGITHLLVAGVTTEVCVQTSMREANDRGYECLVV 180 Query: 181 QDACASYFPEFHRATLDMLTAQSGIVGWRAPLAQLQSAMAAYAGDHP 227 +DACASYFP FHRATLDMLTAQ GIVGWRAPLAQLQSA+ AYAG+HP Sbjct: 181 EDACASYFPAFHRATLDMLTAQGGIVGWRAPLAQLQSAVTAYAGEHP 227 >gi|300310742|ref|YP_003774834.1| cysteine hydrolase falmily protein [Herbaspirillum seropedicae SmR1] Length = 231 Score = 209 bits (531), Expect = 2e-55, Method: Compositional matrix adjust. Identities = 110/226 (48%), Positives = 144/226 (63%), Gaps = 8/226 (3%) Query: 4 IDALPYPYQFH----PRSTALVVIDMQRDFVEEGGFGSALGNDVRPLGAIVPTVAALLTL 59 I A PYP+ + P +TAL+VIDMQ DF GG+ +G D+ A + + +L Sbjct: 6 IQAEPYPWPYDGALTPANTALIVIDMQTDFCGIGGYVDKMGYDLSLTRAPIEPIRKVLAA 65 Query: 60 ARAHQMLVVHTRESHLPDLSDCPRAKRLR----GNPTLGIGDVGPMGRILVRGEPGNQIL 115 RA ++HTRE H PDLSD P KR R G +GIGD GP GRILVRGEPG +I+ Sbjct: 66 MRAGGYTIIHTREGHRPDLSDLPANKRWRSRQIGANGVGIGDAGPCGRILVRGEPGWEII 125 Query: 116 PQLAPIEGEIVIDKPGKGAFYATDLHAQLLERGITHLLIAGVTTEVCVQTSMREANDRGY 175 P+LAPI GEI+IDKPGKG+F ATDL L RGI ++++ G+TT+VCV T+MREANDRG+ Sbjct: 126 PELAPIAGEIIIDKPGKGSFCATDLEMILHTRGIRNIVLTGITTDVCVHTTMREANDRGF 185 Query: 176 ECLVVQDACASYFPEFHRATLDMLTAQSGIVGWRAPLAQLQSAMAA 221 EC+++ D C + H A L M+ Q G+ G A A L + + A Sbjct: 186 ECVMLSDCCGATDYNNHLAALSMIKMQGGVFGAVADSAALIAVIGA 231 >gi|300310658|ref|YP_003774750.1| 2,3-dihydro-2,3-dihydroxybenzoate synthetase [Herbaspirillum seropedicae SmR1] Length = 207 Score = 67.0 bits (162), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 54/177 (30%), Positives = 78/177 (44%), Gaps = 17/177 (9%) Query: 20 LVVIDMQRDFVEEGGFGSALGNDVRPLGAIVPTVAALLTLARAHQMLVVHTRESHLPDLS 79 L+++D F + FG GN + + +LL AR V H+R D + Sbjct: 25 LLIVDFVNSFADPQQFGG--GN----IPQAIERTRSLLAHARQQGWPVAHSRIVFADDDA 78 Query: 80 DCPRAKRLRGNPTLGIGDVGPMGRILVRGEPGNQILPQLAPIEGEIVIDKPGKGAFYATD 139 D + + G G+ L PG+ I+PQLAP GE+V+ K AF+ T Sbjct: 79 D----RNIFGMKVPGM-------VTLKEDLPGSAIVPQLAPANGELVVRKTVPSAFFGTS 127 Query: 140 LHAQLLERGITHLLIAGVTTEVCVQTSMREANDRGYECLVVQDACASYFPEFHRATL 196 L L + G+ LL+AG T CV+ S+ +A G+ LVV D H A L Sbjct: 128 LAPWLTQHGVQTLLVAGAVTSGCVRASVVDAMSWGFRPLVVSDCVGDRALGPHEANL 184 >gi|300313872|ref|YP_003777964.1| 2,3-dihydro-2,3-dihydroxybenzoate synthetase [Herbaspirillum seropedicae SmR1] Length = 176 Score = 54.3 bits (129), Expect = 9e-09, Method: Compositional matrix adjust. Identities = 30/79 (37%), Positives = 46/79 (58%) Query: 105 LVRGEPGNQILPQLAPIEGEIVIDKPGKGAFYATDLHAQLLERGITHLLIAGVTTEVCVQ 164 L RG G Q++P L ++ + K G AF+ T+L A L R I+ L+I G+ TE CV+ Sbjct: 56 LQRGSEGWQLMPGLHQQPEDLHLPKRGSDAFHHTELQAMLKMRHISALVICGMQTEYCVE 115 Query: 165 TSMREANDRGYECLVVQDA 183 +++R A GY ++ DA Sbjct: 116 STVRRALALGYPVMLAADA 134 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.139 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 777,442 Number of extensions: 33812 Number of successful extensions: 89 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 87 Number of HSP's successfully gapped: 5 Length of query: 227 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 145 Effective length of database: 1,231,600 Effective search space: 178582000 Effective search space used: 178582000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)