BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1216.p (964 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310736|ref|YP_003774828.1| isoleucyl-tRNA synthetase [Herb... 1890 0.0 gi|300309767|ref|YP_003773859.1| valyl-tRNA synthetase [Herbaspi... 194 2e-50 >gi|300310736|ref|YP_003774828.1| isoleucyl-tRNA synthetase [Herbaspirillum seropedicae SmR1] Length = 964 Score = 1890 bits (4897), Expect = 0.0, Method: Compositional matrix adjust. Identities = 896/964 (92%), Positives = 934/964 (96%) Query: 1 MSNNESSAKPGKPEKAGKAPSKYPVNMTETAFPMRGDLAKREPQWVKEWQEKKVYQRIRK 60 MSNNESSAKPGKPEKAGKAPSKYPVNMTETAFPMRGDLAKREPQWVK+WQEKKVY+RIRK Sbjct: 1 MSNNESSAKPGKPEKAGKAPSKYPVNMTETAFPMRGDLAKREPQWVKQWQEKKVYERIRK 60 Query: 61 ASAGRPKFILHDGPPYANGDIHIGHAVNKILKDTIVKARNMAGFDAQYVPGWDCHGMPIE 120 ASAGRPKFILHDGPPYANG+IH+GHAVNKILKD IVKARNMAGFDAQYVPGWDCHGMPIE Sbjct: 61 ASAGRPKFILHDGPPYANGEIHLGHAVNKILKDMIVKARNMAGFDAQYVPGWDCHGMPIE 120 Query: 121 IQIEKQFGKNLPVAEVQAKARTYAGEQIERQKADFIRLGVLGEWDNPYKTMNFSNEADEL 180 IQIEK++GKNLPVAEVQAKAR YA EQI QK DFIRLGVLGEWDNPYKTMNFSNEADEL Sbjct: 121 IQIEKKYGKNLPVAEVQAKARAYANEQIALQKKDFIRLGVLGEWDNPYKTMNFSNEADEL 180 Query: 181 RALGKILEKGYVYRGLKPVNWCFDCGSALAEAEVEYQDKRDPAIDVGFPFAEHDKVATAF 240 RALGKILEKGYVYRGLKPVNWCFDCGSALAEAEVEYQDKRDPAIDVGFPFAEHDKVATAF Sbjct: 181 RALGKILEKGYVYRGLKPVNWCFDCGSALAEAEVEYQDKRDPAIDVGFPFAEHDKVATAF 240 Query: 241 GLTELPTRNGYIVIWTTTPWTIPANQALNVHPEFDYALVETTRNGEATLLILAKDLVASC 300 GLT+LPTR+GYIVIWTTTPWTIPANQALNVHPEF+YALVET+RNGEATLLILAKDLVASC Sbjct: 241 GLTDLPTRHGYIVIWTTTPWTIPANQALNVHPEFEYALVETSRNGEATLLILAKDLVASC 300 Query: 301 LERFGLEGKVIATTTGEKLSLIRFHHPLAKADAGYDRLSPVFLGEYVTADSGTGIVHSSP 360 LERFGLEGK+IATTTGEKLSLIRFHHPLAKADAGYDRLSPVFLGEYVTADSGTGIVHSSP Sbjct: 301 LERFGLEGKIIATTTGEKLSLIRFHHPLAKADAGYDRLSPVFLGEYVTADSGTGIVHSSP 360 Query: 361 AYGIEDFISCKAHGMKDDDILNPVMGDGRYASWLPLFAGLTIWEASKPICDKLAEMGSLF 420 AYGIEDF+SCKAHGMKDDDILNPVMGDGRYASWLPLFAGLTIWEASKPICDKL E+GSLF Sbjct: 361 AYGIEDFVSCKAHGMKDDDILNPVMGDGRYASWLPLFAGLTIWEASKPICDKLEEIGSLF 420 Query: 421 KLVMFSHSYMHCWRHKSPIIYRATSQWFASMDNTPKDGGPSLRQTALQGIEDTAFFPGWG 480 KL+MFSHSYMHCWRHKSPIIYRATSQWFASMDNTPKDGGPSLRQTALQGIEDTAFFPGWG Sbjct: 421 KLIMFSHSYMHCWRHKSPIIYRATSQWFASMDNTPKDGGPSLRQTALQGIEDTAFFPGWG 480 Query: 481 KARLHGMIANRPDWTLSRQRQWGVPMAFFVHKETGELHPRTPELIEQVAQRIEKSGIEAW 540 KARLHGMIANRPDWTLSRQRQWGVPMAFFVHKETGELHPRTPEL+EQ+AQRIE+ GIEAW Sbjct: 481 KARLHGMIANRPDWTLSRQRQWGVPMAFFVHKETGELHPRTPELLEQIAQRIEQGGIEAW 540 Query: 541 QALDPKELLGADAEHYVKNRDTLDVWFDSGTTHQTVLRGSHAQQSQFPADLYLEGSDQHR 600 QALDPKELLG +A+ Y+KN+DTLDVWFDSG+THQTVLRGSH Q+ QFPADLYLEGSDQHR Sbjct: 541 QALDPKELLGDEADQYLKNKDTLDVWFDSGSTHQTVLRGSHRQELQFPADLYLEGSDQHR 600 Query: 601 GWFHSSLLTSSMLNGRAPYKALLTHGFVVDGEGKKMSKSKGNVVAPQKVSDSLGAEILRL 660 GWFHSSLLTSSMLNGRAPYKALLTHGFVVDGEG+KMSKS N V PQKV+DSLGAEILRL Sbjct: 601 GWFHSSLLTSSMLNGRAPYKALLTHGFVVDGEGRKMSKSLKNGVEPQKVADSLGAEILRL 660 Query: 661 WVASTDYSGELSISDEILKRVVEAYRRIRNTLRFLLANTSDFDPTQHAVPVAEMLEIDRY 720 WVAS+DYSGE+SIS+EILKRV EAYRRIRNTLRFLLANTSDFDP QHAVP+AE+LEIDRY Sbjct: 661 WVASSDYSGEISISEEILKRVTEAYRRIRNTLRFLLANTSDFDPAQHAVPMAELLEIDRY 720 Query: 721 AIANMARLQAEVLAHFETYEFHPVVAKLQMYCSEDLGGFYLDILKDRLYTSGVESAARRS 780 AIANMARLQ EVLAHFETYEFHPVVAKLQMYCSEDLGGFYLDILKDRLYTSGV+SAARRS Sbjct: 721 AIANMARLQGEVLAHFETYEFHPVVAKLQMYCSEDLGGFYLDILKDRLYTSGVDSAARRS 780 Query: 781 AQSAVWHITQSLLRLMAPILSFTAEEAWRYFASKEAYEASGETIFSQVYYTLPEVADADA 840 AQ+AVWHITQSLLRLMAPILSFTAEEAW +FA KEA+EASGETIFSQVYY LPEVADA Sbjct: 781 AQTAVWHITQSLLRLMAPILSFTAEEAWSFFAGKEAFEASGETIFSQVYYALPEVADAQG 840 Query: 841 LLSKFAVLHEVRAAVTKQLEEVRIAGSIGSSLQAEVELKVSGEKAALLESLGDDLKFVLI 900 LL+KFAVLHEVRAAVTKQLEEVRIAGSIGSSLQAEVELKV+GEKAALLESLGDDLKFVLI Sbjct: 841 LLAKFAVLHEVRAAVTKQLEEVRIAGSIGSSLQAEVELKVAGEKAALLESLGDDLKFVLI 900 Query: 901 TSAARVTPVATAEEEAVVVTPSTHQKCERCWHYRADVGSHADHPGLCGRCYSNLFGQGEP 960 TSAARVT VA+ EEEAVVVTPS HQKCERCWHYRADVGSHA+H GLCGRC SNLFGQGE Sbjct: 901 TSAARVTRVASVEEEAVVVTPSAHQKCERCWHYRADVGSHAEHAGLCGRCVSNLFGQGEK 960 Query: 961 RQFA 964 RQFA Sbjct: 961 RQFA 964 >gi|300309767|ref|YP_003773859.1| valyl-tRNA synthetase [Herbaspirillum seropedicae SmR1] Length = 971 Score = 194 bits (494), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 223/926 (24%), Positives = 377/926 (40%), Gaps = 178/926 (19%) Query: 39 AKREPQWVKEWQEKKVYQRIRKASAGRPKFILHDGPPYANGDIHIGHAVNKILKDTIVKA 98 A+ E W +EW+++ + A +P F + PP G +H+GHA N+ + D + + Sbjct: 45 AEIEKFWREEWEKRGYFAAT--TDAEKPSFSIQLPPPNVTGTLHMGHAFNQTIMDGLTRY 102 Query: 99 RNMAGFDAQYVPGWDCHGMPIEIQIEKQF----------GKNLPVAEVQAKARTYAGEQI 148 M G++ ++PG D G+ +I +E+Q G+ V +V + + +G I Sbjct: 103 HRMRGYNTAWIPGTDHAGIATQIVVERQLDAQKVSRHDLGREKFVEKVW-EWKEKSGSTI 161 Query: 149 ERQKADFIRLGVLGEWDNPYKTMNFSNEADELRALGKILEKGYVYRGLKPVNWCFDCGSA 208 Q R+G +W Y TM+ ++ E+G +YRG + VNW G+A Sbjct: 162 TGQ---MRRMGASTDWAREYFTMDPKMSKVVTEVFVRLYEQGLIYRGKRLVNWDPVLGTA 218 Query: 209 LAEAEVEYQDKRDPAIDVGFPFAEHDKVATAFGLTELPTRNGYIVIWTTTPWTIPANQAL 268 +++ EV +++ + +P A+ +G+IV+ TT P T+ + A+ Sbjct: 219 VSDLEVVSEEEDGSMWHIKYPLADG---------------SGHIVVATTRPETMLGDVAV 263 Query: 269 NVHPEFDYALVETTRNGEATLLILAKDLVASCLERFG-LEGKVIATTTGEKLSLIRFHHP 327 V P ER+ L GK+I KL L+ Sbjct: 264 AVDP---------------------------TDERYAALVGKMI------KLPLV----- 285 Query: 328 LAKADAGYDRLSPVFLGEYVTADSGTGIVHSSPAYGIEDFISCKAHGMKDDDILNPVMGD 387 R P+ EYV + GTG V +PA+ D+ H ++ + Sbjct: 286 --------GREIPIIKDEYVDKEFGTGCVKITPAHDFNDYQVGARHNLEMISVFTLKATI 337 Query: 388 GRYASWLPLFAGLTIWEASKPICDKLAEMGSLFKLVMFSHSYMHCWRHKSPIIYRA--TS 445 A + GL +EA K I L +G L ++ H M ++ ++ T Sbjct: 338 NENAP--EKYQGLDRFEARKQIVADLDALGLLEQVK--PHKLMVPRGDRTGVVIEPMLTD 393 Query: 446 QWFASMDNTPKDG----GPSLRQTALQGIE--DTAFFPGWGKARLHGMIANRPDWTLSRQ 499 QWF +M +G G S+ +TAL+ + + F P + + N DW +SRQ Sbjct: 394 QWFVAMSKPAPEGTFFPGKSIAETALEKVSSGEIKFVPENWTNTYNQWLNNIQDWCISRQ 453 Query: 500 RQWGVPMAFFVHKETGELHPRTPELIEQVAQRIEKSGIEAWQALDPKELLGADAEHYVKN 559 WG + + ++ G RT E E AQ A ++ Sbjct: 454 LWWGHQIPAWYDEDGGIYVARTEE--EARAQ--------------------AGGRAVKRD 491 Query: 560 RDTLDVWFDSGTTHQTVL---RGSHAQQSQFPADLYLEGSDQHRGWFHSSLLTSSMLNGR 616 D LD WF S + L + + P+ + + G D W ++ ++ G+ Sbjct: 492 EDVLDTWFSSALVPFSTLGWPEETPDYKLFLPSSVLVTGFDIIFFWVARMVMMTTHFTGK 551 Query: 617 APYKALLTHGFVVDGEGKKMSKSKGNVVAPQKVSDSLGAEILRL----------WVASTD 666 P+ + HG V D G+KMSKSKGN + P + D +G + L AS + Sbjct: 552 VPFDTVYVHGLVRDASGQKMSKSKGNTLDPIDLIDGIGVDELVAKRTVGLMNPKQAASIE 611 Query: 667 YSGELSISDEI-----------------LKRVV-------EAYRRIRNTL----RFLLAN 698 + +D I L R + E YR N L RF+L N Sbjct: 612 KATRKEFADGIPAFGTDALRFTFASLATLGRNINFDLNRCEGYRNFCNKLWNATRFVLMN 671 Query: 699 TS----DFDPTQHAVPVAEMLEI---DRYAIANMARLQAEVLAHFETYEFHPVVAKLQMY 751 T FD + V E L+ DR+ ++ + R +AEV F Y F + + + Sbjct: 672 TEGQDCGFDGHEKGVCDKEKLQFSKADRWIVSLLQRTEAEVEKGFADYRFDNIATAIYKF 731 Query: 752 CSEDLGGFYLDILKDRLYTSGVESAARRSAQSAVWHITQSLLRLMAPILSFTAEEAWRYF 811 ++ +YL++ K ++ SG E A +R+ + + + +S+LRL P+L F E+ W+ Sbjct: 732 VWDEYCDWYLEMAKAQI-QSGNE-AQQRATRRTLLRVLESVLRLAHPVLPFITEQLWQSV 789 Query: 812 A--SKEAYEASGETIFSQVYYTLPEV----ADADALLSKFAVLHEVRAAVTKQLEEVRIA 865 A + + +G++I Q Y P++ A+A+A +++F A T +R Sbjct: 790 APLTDRKPDPAGDSIMRQA-YPRPDLEKIDAEAEAWVAQF-------KAYTDACRNLRGE 841 Query: 866 GSIGSSLQAEVELKVSGEKAALLESL 891 + SL+ V L V A+ +ES Sbjct: 842 MQLAPSLR--VPLIVEAADASAVESF 865 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,947,767 Number of extensions: 124526 Number of successful extensions: 334 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 329 Number of HSP's successfully gapped: 4 Length of query: 964 Length of database: 1,619,870 Length adjustment: 94 Effective length of query: 870 Effective length of database: 1,174,780 Effective search space: 1022058600 Effective search space used: 1022058600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 135 (56.6 bits)