BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1165.p (503 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipo... 841 0.0 gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipo... 501 e-143 gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbas... 422 e-119 gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipo... 354 9e-99 gi|300311271|ref|YP_003775363.1| outer membrane drug efflux lipo... 230 3e-61 >gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 498 Score = 841 bits (2172), Expect = 0.0, Method: Compositional matrix adjust. Identities = 419/484 (86%), Positives = 454/484 (93%), Gaps = 3/484 (0%) Query: 23 LLPLLLAVLLGGCTLMPDYHRPDAPVAARFAG---DNSAPAAQSAAVADIGWRDVFTDPA 79 +LP++LA+LLGGCTLMPDYHRPDAPVAA FAG N+A A+ + VADIGWRDVFTDP+ Sbjct: 15 MLPVMLAMLLGGCTLMPDYHRPDAPVAAHFAGADSKNAAGASSAPVVADIGWRDVFTDPS 74 Query: 80 LQQVIAMSLANNRDLRVAVLNIEKARAQYGVERAALFPTINAATSESASRTPADLSTTGS 139 LQQVIA++L NNRDLRVAVLNIEKARAQYGV+RAALFP+I AATSE+ASRTP DLS+TGS Sbjct: 75 LQQVIALALENNRDLRVAVLNIEKARAQYGVQRAALFPSIKAATSETASRTPGDLSSTGS 134 Query: 140 ALTARSYSATLGFSAYELDLFGRVRSLKEQALQSFLSTAEARRSTQISLIAEVATAWLTL 199 ALT+RSY+ATLGFSAYELDLFGRVRSLKEQALQSFLSTAEARRSTQISLIAEVATAWLTL Sbjct: 135 ALTSRSYTATLGFSAYELDLFGRVRSLKEQALQSFLSTAEARRSTQISLIAEVATAWLTL 194 Query: 200 ASDQDRLRLAKDTLQSQSATYQLTQRSVELGSSSALTLRQVQTSVESARVDVESYTAQVD 259 ASDQDRLRLA+DTL+SQS++Y LTQRS ELGSSSALTLRQ QTSVESARVDVESYTAQVD Sbjct: 195 ASDQDRLRLAQDTLESQSSSYALTQRSFELGSSSALTLRQAQTSVESARVDVESYTAQVD 254 Query: 260 QDRNALVLLVGKDLPDTLLPHGLPDTALAAASPLATLPPGLPSELLQRRADIVQAERDLK 319 QDRNALVLLVGKDLP LLP GLPDT LAAASPLAT+PPGLPS+LLQRRADIVQAERDL+ Sbjct: 255 QDRNALVLLVGKDLPTALLPQGLPDTRLAAASPLATIPPGLPSDLLQRRADIVQAERDLR 314 Query: 320 AANANIGAARAAFYPSISLTADAGTASARLAGLFKGGSGSWSFVPQISLPIFDGGANRAN 379 AANANIGAARAAFYPSI+LT +AGTASARLAGLFKGGSGSWSFVPQISLPIFDGGAN+AN Sbjct: 315 AANANIGAARAAFYPSITLTVNAGTASARLAGLFKGGSGSWSFVPQISLPIFDGGANQAN 374 Query: 380 LDIATISRDISVAQYEKAIQTAFREVSDALAQRNTLGRQLAAQESLVEASGAAYDLSQAR 439 LDIAT+SRDISVAQYEKAIQTAFREVSDALAQRNTLGRQL AQE+LV+AS A+ LSQAR Sbjct: 375 LDIATVSRDISVAQYEKAIQTAFREVSDALAQRNTLGRQLQAQEALVQASDEAFRLSQAR 434 Query: 440 FKSGVDSYLDVLDSQRSLYSAQSNLIGTRLSRWTNLVTFYKVLGGGWVERTGTPVASDVA 499 + SGVDSYL VLDSQR+LYSAQ NLIGT+LSRWTNLVTFYK LGGGWVERTGTP ASD+A Sbjct: 435 YHSGVDSYLTVLDSQRTLYSAQKNLIGTQLSRWTNLVTFYKTLGGGWVERTGTPAASDIA 494 Query: 500 TAQP 503 T +P Sbjct: 495 TQEP 498 >gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 497 Score = 501 bits (1291), Expect = e-143, Method: Compositional matrix adjust. Identities = 273/466 (58%), Positives = 336/466 (72%), Gaps = 13/466 (2%) Query: 34 GC-TLMPDYHRPDAPV--------AARFAGDNSAPAAQSAAVADIGWRDVFTDPALQQVI 84 GC L PDY +P APV AA+ A ++ PAA +GWR+ F DP LQQVI Sbjct: 27 GCANLAPDYTQPAAPVPASWTSQAAAQPAVGSAGPAAGEIDADHLGWREFFLDPRLQQVI 86 Query: 85 AMSLANNRDLRVAVLNIEKARAQYGVERAALFPTINAATSESASRTPADLSTTGSALTAR 144 A +L NNRDLRVA LNIEKARAQY + A +P+I+A + SASRTPA+LSTTGSA + Sbjct: 87 AAALDNNRDLRVAALNIEKARAQYRITDANRYPSISATGTSSASRTPAELSTTGSAAVSH 146 Query: 145 SYSATLGFSAYELDLFGRVRSLKEQALQSFLSTAEARRSTQISLIAEVATAWLTLASDQD 204 S T+G SAYELD FGR+++L + AL+S+ E RRST ISL+AEVATAWLTLASD+D Sbjct: 147 QQSVTVGVSAYELDFFGRLKNLSDAALESYQYQVETRRSTHISLVAEVATAWLTLASDRD 206 Query: 205 RLRLAKDTLQSQSATYQLTQRSVELGSSSALTLRQVQTSVESARVDVESYTAQVDQDRNA 264 L+LA+DT SQS T+ L+QRS ++G+ S + L + +VESAR DV Y +QV+QD NA Sbjct: 207 LLKLAQDTYASQSRTFALSQRSHDIGTVSGVDLASQEATVESARADVARYASQVEQDLNA 266 Query: 265 LVLLVGKDLPDTLLPHGLPDTALAAASPLATLPPGLPSELLQRRADIVQAERDLKAANAN 324 L LLVG D+ LLP GLPDT AS L P GLPS++L RR D++ AE LK+ANA+ Sbjct: 267 LRLLVGADIDAALLPAGLPDT----ASVLPATPAGLPSQVLLRRPDVLAAEHTLKSANAD 322 Query: 325 IGAARAAFYPSISLTADAGTASARLAGLFKGGSGSWSFVPQISLPIFDGGANRANLDIAT 384 IGAARAAF+PSISLTA+ GTAS L GLFK GSGSW+F P I+LPIF+ G+ RA+LD A Sbjct: 323 IGAARAAFFPSISLTANGGTASRTLGGLFKAGSGSWTFSPSINLPIFNAGSLRASLDSAK 382 Query: 385 ISRDISVAQYEKAIQTAFREVSDALAQRNTLGRQLAAQESLVEASGAAYDLSQARFKSGV 444 I+RDI VA YEKAIQTAFREV+DALA R TL ++L AQ AS A+ LS AR+++GV Sbjct: 383 ITRDIDVANYEKAIQTAFREVADALAVRATLDQRLDAQGKATAASEKAFRLSDARYRNGV 442 Query: 445 DSYLDVLDSQRSLYSAQSNLIGTRLSRWTNLVTFYKVLGGGWVERT 490 DSYL L +QR+LYSAQ LI RL+ +N +T YKVLGGGW E+T Sbjct: 443 DSYLTTLVAQRTLYSAQQTLISLRLTEQSNRITLYKVLGGGWSEQT 488 >gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 483 Score = 422 bits (1086), Expect = e-119, Method: Compositional matrix adjust. Identities = 237/465 (50%), Positives = 308/465 (66%), Gaps = 7/465 (1%) Query: 26 LLLAVLLGGCTLMPDYHRPDAPVAARFAGDNSAPAAQSAAVADIGWRDVFTDPALQQVIA 85 L++A +LGGC+L P Y RP APV + + D +A A+ AV ++GWR F D LQQ+IA Sbjct: 19 LVMAGVLGGCSLAPTYERPQAPVESSYPAD-AAGRAELQAV-NLGWRQFFPDQRLQQLIA 76 Query: 86 MSLANNRDLRVAVLNIEKARAQYGVERAALFPTINAATSESASRTPADLSTTGSALTARS 145 +L NNRDLR AVLNIE+ARAQY + RA L P +N + + + +RT A S TG + S Sbjct: 77 AALENNRDLRTAVLNIEEARAQYQIARADLLPNLNLSGNAARTRTAASQSLTGQPTISNS 136 Query: 146 YSATLGFSAYELDLFGRVRSLKEQALQSFLSTAEARRSTQISLIAEVATAWLTLASDQDR 205 Y L AYELD FGRVR+LK+ AL S+LST EA++S QISL+A+VA A+L S ++ Sbjct: 137 YQVGLAVPAYELDFFGRVRNLKDAALASYLSTEEAQKSAQISLVAQVAQAYLAERSYAEQ 196 Query: 206 LRLAKDTLQSQSATYQLTQRSVELGSSSALTLRQVQTSVESARVDVESYTAQVDQDRNAL 265 L+LA +TL+S+ + L Q+ +++G+S+AL R QT VESARV Q Q NAL Sbjct: 197 LKLADETLKSRQSNLDLAQKRLDVGASTALDFRLTQTLVESARVAKTQLERQRAQAENAL 256 Query: 266 VLLVGKDLPDTLLPHGLPDTALAAASPLATLPPGLPSELLQRRADIVQAERDLKAANANI 325 LLVGK + + LP P L+ + +P GLPS+LL R DI AE+ L AANANI Sbjct: 257 TLLVGKKIGE--LP---PAQLLSDEKIVTDIPAGLPSDLLTNRPDIRSAEQSLLAANANI 311 Query: 326 GAARAAFYPSISLTADAGTASARLAGLFKGGSGSWSFVPQISLPIFDGGANRANLDIATI 385 GAARAAF+P ISLT + G+AS+ L GLF+ GSG+WSF P +SLPIFD G N ANLD+A + Sbjct: 312 GAARAAFFPRISLTGNFGSASSTLGGLFESGSGAWSFGPSLSLPIFDFGRNSANLDLAEV 371 Query: 386 SRDISVAQYEKAIQTAFREVSDALAQRNTLGRQLAAQESLVEASGAAYDLSQARFKSGVD 445 ++ +V YEKAIQTAFREV+DAL R +L Q+ AQE+ + A L+ RFK+G+ Sbjct: 372 RKNKAVVTYEKAIQTAFREVADALVARGSLDEQVKAQEAFLAAQQERLKLTDLRFKNGIA 431 Query: 446 SYLDVLDSQRSLYSAQSNLIGTRLSRWTNLVTFYKVLGGGWVERT 490 S LD LD+QR L+SAQ LI R R N + YK LGGG E T Sbjct: 432 SSLDQLDAQRDLFSAQQALIQARQLRLNNAIDLYKALGGGLNENT 476 >gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 491 Score = 354 bits (909), Expect = 9e-99, Method: Compositional matrix adjust. Identities = 210/455 (46%), Positives = 281/455 (61%), Gaps = 10/455 (2%) Query: 35 C-TLMPDYHRPDAPVAARF----AGDNSAPAAQSAAVADIGWRDVFTDPALQQVIAMSLA 89 C +L P Y RP APVAA + + + A AA + WRD FTDP LQ +I +L Sbjct: 25 CQSLAPHYQRPAAPVAAVYPDGPVNEAAHQVANEAASPPLPWRDYFTDPPLQALITQALE 84 Query: 90 NNRDLRVAVLNIEKARAQYGVERAALFPTINAATSESASRTPADLSTTGSALTARSYSAT 149 N+RDLR A L +++A+A YG+ A LFPTI A SRTPADL+ T L +Y A Sbjct: 85 NSRDLRTAALRVQEAQALYGIGHADLFPTIGAQAGLDRSRTPADLNLTRRPLVGSAYQAG 144 Query: 150 LGFSAYELDLFGRVRSLKEQALQSFLSTAEARRSTQISLIAEVATAWLTLASDQDRLRLA 209 LG S++E+DL+GR+RSL + AL+S ++ R + +SL+A+VA AWL LA +RL +A Sbjct: 145 LGLSSWEIDLWGRLRSLDDAALESARASQATRSAVALSLMAQVADAWLELAELDERLEIA 204 Query: 210 KDTLQSQSATYQLTQRSVELGSSSALTLRQVQTSVESARVDVESYTAQVDQDRNALVLLV 269 T+ S+ ++++ R VE+G++S L L Q++T + A+ Q Q NAL LLV Sbjct: 205 NQTIASRQESFRIFSRRVEVGATSRLNLTQIETLLTQAQALGAQLQQQRAQRLNALTLLV 264 Query: 270 GKDLPDTLLPHGLPDTALAAASPLATLPPGLPSELLQRRADIVQAERDLKAANANIGAAR 329 G + +LP G L A A L PGLP+ LL RR D+ AE L AANANIGAAR Sbjct: 265 GSPV---VLPVGQARAGLDAV--FAPLQPGLPALLLTRRPDVAAAEHRLMAANANIGAAR 319 Query: 330 AAFYPSISLTADAGTASARLAGLFKGGSGSWSFVPQISLPIFDGGANRANLDIATISRDI 389 AAF+PSISLT + GTASA L+GLF+ GS +W+F P I+LPIF G R NL ++ + RD+ Sbjct: 320 AAFFPSISLTGNVGTASAELSGLFRDGSHAWTFSPSITLPIFTAGRLRNNLALSEVRRDL 379 Query: 390 SVAQYEKAIQTAFREVSDALAQRNTLGRQLAAQESLVEASGAAYDLSQARFKSGVDSYLD 449 +VA YEK +QTAFREV+DALA R L +QL +S V LS R+ +G +LD Sbjct: 380 AVAAYEKTVQTAFREVADALAARQWLTQQLEIAQSAVRVQRERARLSALRYDNGAAPFLD 439 Query: 450 VLDSQRSLYSAQSNLIGTRLSRWTNLVTFYKVLGG 484 VLD+QR L SAQ L+ R + ++ V Y LGG Sbjct: 440 VLDAQRDLLSAQQQLVQIRRALLSSSVALYAALGG 474 >gi|300311271|ref|YP_003775363.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 481 Score = 230 bits (586), Expect = 3e-61, Method: Compositional matrix adjust. Identities = 163/430 (37%), Positives = 234/430 (54%), Gaps = 9/430 (2%) Query: 58 APAAQSAAVADIGWRDVFTDPALQQVIAMSLANNRDLRVAVLNIEKARAQYGVERAALFP 117 APA + + IGW+D F LQ +IA +L +N DLR L +++ +AQ+ ++R+ P Sbjct: 46 APANAAPTASAIGWQDYFPQAPLQALIAEALRHNPDLRDIRLRLQQVQAQWRIQRSTGLP 105 Query: 118 TINAATSESASRTPADLSTTGSALTARSYSATLGFSAYELDLFGRVRSLKEQALQSFLST 177 T+N SR P + +G A + R Y LG S++ELD +GR+ SL E ALQ ++++ Sbjct: 106 TVNLEAQARRSRGPLEPPESGPAASTREYRVGLGASSWELDFWGRIASLNEAALQEWMAS 165 Query: 178 AEARRSTQISLIAEVATAWLTLASDQDRLRLAKDTLQSQSA-TYQLTQRSVELGSSSALT 236 A ++ ISLI +VA AW +L +RL L +TLQ+ +A + L Q + G + A Sbjct: 166 RAAEQALVISLIGQVAAAWTSLRELDERLHLL-ETLQAATAESLALMQARHDAGRADAAA 224 Query: 237 LRQVQTSVESARVDVESYTAQVDQDRNALVLLVG-KDLPDTLLPHGLPDTALAAASPLAT 295 L Q ++ + R + R+ L +L G LP L P P T L P A Sbjct: 225 LAQQRSELAQMRAEQAELQQMRASQRSTLEMLAGLTQLPAALEP--APTTILL---PFA- 278 Query: 296 LPPGLPSELLQRRADIVQAERDLKAANANIGAARAAFYPSISLTADAGTASARLAGLFKG 355 L PGLPS LL R DI+ AE L+A+ A + AARAAF+P ISLTA G AS L LFKG Sbjct: 279 LAPGLPSTLLAARPDIIAAEHRLQASAAQVAAARAAFFPRISLTAFGGVASTALQDLFKG 338 Query: 356 GSGSWSFVPQISLPIFDGGANRANLDIATISRDISVAQYEKAIQTAFREVSDALAQRNTL 415 S +W+FVP+ISLP+ DGG ++ DIA + + ++ YE+ +Q AFREV+ L + + L Sbjct: 339 PSRAWNFVPEISLPLLDGGLRQSRYDIAALDQQRALLDYERTVQNAFREVAQRLEEAHWL 398 Query: 416 GRQLAAQESLVEASGAAYDLSQARFKSGVDSYLDVLDSQRSLYSAQSNLIGTRLSRWTNL 475 QL + +V+ R + G S LDVL+S+R AQ + N Sbjct: 399 KEQLRLRREVVQQQAQRQAQMHMRLRYGRASRLDVLESERDHLRAQQRQHMLEHALLRNQ 458 Query: 476 VTFYKVLGGG 485 + Y LGGG Sbjct: 459 IALYVALGGG 468 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.128 0.353 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,178,404 Number of extensions: 40205 Number of successful extensions: 270 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 213 Number of HSP's successfully gapped: 18 Length of query: 503 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 414 Effective length of database: 1,198,455 Effective search space: 496160370 Effective search space used: 496160370 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)