BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1156.p (349 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310685|ref|YP_003774777.1| 2-ketopantoate reductase [Herba... 585 e-168 gi|300310480|ref|YP_003774572.1| 2-dehydropantoate 2-reductase [... 109 3e-25 gi|300311176|ref|YP_003775268.1| 2-dehydropantoate 2-reductase [... 85 1e-17 >gi|300310685|ref|YP_003774777.1| 2-ketopantoate reductase [Herbaspirillum seropedicae SmR1] Length = 353 Score = 585 bits (1509), Expect = e-168, Method: Compositional matrix adjust. Identities = 299/337 (88%), Positives = 314/337 (93%), Gaps = 3/337 (0%) Query: 1 MSDDS---SKHLAPLRICIAGAGAIGGTLAVRLSAAGHQVSVLARGQTLQAIREHGLTLH 57 MSDDS S APLRICIAGAGAIGGTLAVRL+AAGHQVSVLARGQTLQAIREHGLTLH Sbjct: 1 MSDDSISPSAPSAPLRICIAGAGAIGGTLAVRLAAAGHQVSVLARGQTLQAIREHGLTLH 60 Query: 58 DLHGRTHARPTASELPEFGEQDVIFLCAKSQDMSALLSQIAPLVGAQTVVIPTNNGVPWW 117 DLHG HARP A P FG QDVIFLCAKSQDM+ LL Q+AP+VG TVVIPTNNGVPWW Sbjct: 61 DLHGTAHARPAADHRPSFGVQDVIFLCAKSQDMAGLLPQVAPMVGETTVVIPTNNGVPWW 120 Query: 118 YFHREGGRFDGQTVKAVDPDGALSRAVPLAQLIGSVLFITAEVERPGVIRSVNPHLMVLG 177 YFHREGGRFDGQTVKAVDP+GAL+ A+PLAQ+IGSVLFITAEVE GVIRSVNPHLMVLG Sbjct: 121 YFHREGGRFDGQTVKAVDPEGALAAALPLAQIIGSVLFITAEVESAGVIRSVNPHLMVLG 180 Query: 178 EPSGEMSERLLRVRAAVEAAGIEARATDRIRDKLWTKIIANISTNPLSVITRGTLEQLYG 237 EPSGEMSERLLRVRAAVE+AGIEARATDRIRDKLWTKIIANISTNPLSVIT+GTL+QLYG Sbjct: 181 EPSGEMSERLLRVRAAVESAGIEARATDRIRDKLWTKIIANISTNPLSVITQGTLQQLYG 240 Query: 238 QPELREVVSQIMRETLLVASSYGARVDIDPLTFLQLGEAMGAFRTSMLQDLERGRPLELA 297 PELREVVSQIMRETLLVASS+GARVDIDPLTFLQLGEAMGAFRTSMLQDLERGRPLELA Sbjct: 241 LPELREVVSQIMRETLLVASSHGARVDIDPLTFLQLGEAMGAFRTSMLQDLERGRPLELA 300 Query: 298 AIGDAVLEMAERFAIPMPITRAVVSLARFRGETARQQ 334 AIGDAVLEMAER+AIPMPITRA+VSLARFRGE A+QQ Sbjct: 301 AIGDAVLEMAERYAIPMPITRAIVSLARFRGEAAQQQ 337 >gi|300310480|ref|YP_003774572.1| 2-dehydropantoate 2-reductase [Herbaspirillum seropedicae SmR1] Length = 318 Score = 109 bits (272), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 96/324 (29%), Positives = 153/324 (47%), Gaps = 34/324 (10%) Query: 12 LRICIAGAGAIGGTLAVRLSAAGHQVSVLARG-QTLQAIREHGLTLHDLHGRTHARPTAS 70 ++I I GAGA+GG A RL+ AG VS++ +++AI HGL+L D G R Sbjct: 1 MKIAIVGAGAMGGLFAARLALAGQDVSLIEVAPASIEAISSHGLSLTDEDGAHTVRVPVG 60 Query: 71 ELPEFGEQ-DVIFLCAKSQDMSALLSQIAPLVGAQTVVIPTNNGVPWWYFHREGGRFDGQ 129 F + D+I L K A ++ +A L G T + NG+ G D Sbjct: 61 RADSFQDAFDLIILFTKGFHTQAAIASVAHLAGPHTWALSVQNGL---------GNAD-- 109 Query: 130 TVKAVDPDGALSRAVPLAQLIGSVLFITAEVERPGVIRSVNPHLMVLGEPSGEMSERLLR 189 ++ +P ++I + + A++ PGV+ S + + SG+ S +L + Sbjct: 110 ---------LIAEVIPRERIIVGMTNLPADLRAPGVVHSHGEGQVRIWTLSGQPSAQLTQ 160 Query: 190 VRAAVEAAGIEARATDRIRDKLWTKIIANISTNPLSVITRGTLEQLYGQPELREVVSQIM 249 V A AAG+ A ++ +W K+ N + N + +T T+ RE+ +I Sbjct: 161 VAADFNAAGLPCSADPLVQQAIWEKLAFNAALNAICAVTGLTVGAAGASASGRELALRIA 220 Query: 250 RETLLVASSYGARVDIDPLTFLQLGEAM-GAFRT------SMLQDLERGRPLELAAIGDA 302 ET+ VA + G ID +Q+ +A+ AFRT SMLQD+E GR E+ I A Sbjct: 221 AETVAVAQAAG----IDA-RLVQVEDAIEHAFRTHLHHKPSMLQDIEAGRRTEIDFINGA 275 Query: 303 VLEMAERFAIPMPITRAVVSLARF 326 V+ AER +P+P T+ + L + Sbjct: 276 VVREAERLGLPVPTTQTLHQLVKL 299 >gi|300311176|ref|YP_003775268.1| 2-dehydropantoate 2-reductase [Herbaspirillum seropedicae SmR1] Length = 297 Score = 84.7 bits (208), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 78/318 (24%), Positives = 145/318 (45%), Gaps = 30/318 (9%) Query: 12 LRICIAGAGAIGGTLAVRLSAAGHQVSVLARGQTLQAIREHGLTLH-DLHGRTHARPTAS 70 +++ + GAGA+G A L+ GHQV+++ R Q ++A GL L T A ++ Sbjct: 1 MKVAVMGAGAVGCYFAGMLARVGHQVALVGRPQHVEAFNTRGLRLETKAFDETIAVKAST 60 Query: 71 ELPEFGEQDVIFLCAKSQDMSALLSQIAPLVGAQTVVIPTNNGVPWWYFHREGGRFDGQT 130 + + +++ C KS D +Q+AP + T+V+ NG Sbjct: 61 DAAIAADAELVLFCVKSPDTETAGAQLAPHLAPDTLVLSLQNGTD--------------- 105 Query: 131 VKAVDPDGALSRAVPLAQLIGSVLFITAEVERPGVIRSVNPHLMVLGEPSGEMSERLLRV 190 + R V + +V+++ E+ PG ++ +V+ EP+ + Sbjct: 106 ------NAERLRTVMAQPVAAAVVYVATEMAGPGHVKHHGRGELVI-EPAAGSRD----A 154 Query: 191 RAAVEAAGIEARATDRIRDKLWTKIIANISTNPLSVITRGTLEQLYGQPELREVVSQIMR 250 A+ AAG+ +D +R +LW K+I N + N LS I + +L P + EV++Q++ Sbjct: 155 ADALVAAGVPTEISDNVRGELWLKLILNCAYNALSAIAQLPYGKLVEGPGIPEVIAQLVT 214 Query: 251 ETLLVASSYGARV--DIDPLTFLQLGEAMGAFRTSMLQDLERGRPLELAAIGDAVLEMAE 308 E VA++ G + D+D ++ + M +S QDL RG+P E+ + ++ A+ Sbjct: 215 ECKAVAAAEGVTLADDVDS-AVRKIVQTMPGQFSSTAQDLARGKPSEIDHLNGFIVARAQ 273 Query: 309 RFAIPMPITRAVVSLARF 326 + P V +L + Sbjct: 274 AHGLSAPANLTVHALVKL 291 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 932,365 Number of extensions: 36467 Number of successful extensions: 150 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 146 Number of HSP's successfully gapped: 3 Length of query: 349 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 263 Effective length of database: 1,212,660 Effective search space: 318929580 Effective search space used: 318929580 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)