BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1145.p (391 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310674|ref|YP_003774766.1| amidase family protein [Herbasp... 508 e-145 gi|300311162|ref|YP_003775254.1| amidase family protein [Herbasp... 145 4e-36 gi|300311728|ref|YP_003775820.1| indole acetamide hydrolase [Her... 109 5e-25 gi|300309904|ref|YP_003773996.1| aspartate tRNAAsn/Glu-tRNAGln a... 96 7e-21 gi|300310745|ref|YP_003774837.1| amidase family protein [Herbasp... 86 5e-18 >gi|300310674|ref|YP_003774766.1| amidase family protein [Herbaspirillum seropedicae SmR1] Length = 388 Score = 508 bits (1307), Expect = e-145, Method: Compositional matrix adjust. Identities = 322/387 (83%), Positives = 352/387 (90%) Query: 1 MNNSAPRTIAGSGNALLSRFHLGGNGPTVAIKDCIDIAGQPTCGGSAALADAPPAQSHAE 60 MN + PRT+ G+GNALLSRFHLGG+GPTVAIKDCIDIAGQPTCGGSAALADAPPA +HA+ Sbjct: 1 MNAATPRTVPGTGNALLSRFHLGGSGPTVAIKDCIDIAGQPTCGGSAALADAPPASAHAD 60 Query: 61 VVQRLLDTGWQIIARANMHELAYGMTGINDWNGTPVNPQDPGRMTGGSSSGSASAVGAGL 120 VV RLL+ GWQI ARANMHELAYGMTGIN+WNGT VNPQDP MTGGSSSGSASAVGAGL Sbjct: 61 VVARLLEAGWQITARANMHELAYGMTGINEWNGTAVNPQDPACMTGGSSSGSASAVGAGL 120 Query: 121 VDLSIGSDTGGSIRLPAACCGVIGLKPTFGRVSRAGAYPAISSLDCVGPVARSMEMIIRA 180 VD+SIGSDTGGSIRLPAACCGVIG KP+FGRVSRAGAYPA+SSLDC+GP +RSM++II A Sbjct: 121 VDVSIGSDTGGSIRLPAACCGVIGFKPSFGRVSRAGAYPALSSLDCLGPFSRSMDLIISA 180 Query: 181 MTSIAPGFDRVAASAAAAHARVKRVTVQADDDVAAAVAQAFSASRWSATWWTPQHMHAAF 240 M SI PGFDR AAS AAHARVK VTVQAD ++A AVA AF+AS W + WTPQHM AAF Sbjct: 181 MASITPGFDRAAASTPAAHARVKLVTVQADTEIAEAVAAAFAASGWEGSPWTPQHMQAAF 240 Query: 241 EAGMTLINRETHDAFGHLLGQGKLGADIEQRLAAAATTTDAAVADAERVRAAFSAEVDAA 300 +AGMTLINRETHDAFGHLLG GKLGADIE+RL+AAA+T+DAAVA AE VR AFSAE+DAA Sbjct: 241 DAGMTLINRETHDAFGHLLGLGKLGADIEKRLSAAASTSDAAVAHAETVRTAFSAEIDAA 300 Query: 301 LADADALLLPTLPALPPTLQAIRQGTSVLLLSSLVRPFNLSGHPALTIPVPIAGSTLKAG 360 LADADALLLPTLPALPPTL+AIRQGTSVL+LSSLVRPFNLSGHPAL+IPVPIAGS LKAG Sbjct: 301 LADADALLLPTLPALPPTLEAIRQGTSVLMLSSLVRPFNLSGHPALSIPVPIAGSPLKAG 360 Query: 361 LQLVGRKGDDERLCALGRHLEQALQAL 387 LQLVGRKG+DERLCALG HLEQ L+ L Sbjct: 361 LQLVGRKGEDERLCALGLHLEQVLKRL 387 >gi|300311162|ref|YP_003775254.1| amidase family protein [Herbaspirillum seropedicae SmR1] Length = 450 Score = 145 bits (367), Expect = 4e-36, Method: Compositional matrix adjust. Identities = 128/384 (33%), Positives = 184/384 (47%), Gaps = 25/384 (6%) Query: 25 NGPTVAIKDCIDIAGQPTCGGSAALADAPPAQSHAEVVQRLLDTGWQIIARANMHELAYG 84 G V++KD D+AG+ T GS L P A +HA VVQ LL G +I + NM E AY Sbjct: 70 EGVPVSVKDLFDVAGETTLAGSVVLRGKPVASAHAAVVQNLLKAGAILIGKTNMTEFAYS 129 Query: 85 MTGINDWNGTPVNPQDP----GRMTGGSSSGSASAVGAGLVDLSIGSDTGGSIRLPAACC 140 GIN GTP N + GR+ GGSSSG+A +V G+ ++GSDTGGS+R+P+A Sbjct: 130 GLGINPHYGTPQNAWERNIEGGRIPGGSSSGAAISVTDGMAFAAVGSDTGGSVRIPSALN 189 Query: 141 GVIGLKPTFGRVSRAGAYPAISSLDCVGPVARSMEMIIRAMTSIAPGFDRVAASAAAAHA 200 G+ G KPT RVS G P + LD +GP+A S++ M ++ G D VA A Sbjct: 190 GLTGFKPTAARVSMQGVLPLSAYLDSIGPLAVSVQ-CCAIMDAVLAGEDYVAVLAHPLRG 248 Query: 201 -RVKRVTVQADDDVAAAVAQAFSASRWSATWWTPQHMHA---AFEAGMTLIN-------R 249 R+ T D + V QA+ + + A AF A + IN Sbjct: 249 LRLLAPTNVVLDGMDDTVGQAYRTALGKLAAAGAMIVEAEVPAFNA-LAAINAKGGFTAA 307 Query: 250 ETHDAFGHLLGQGKLGAD--IEQRLAAAATTTDAAVADAERVRAAFSAEVDAALADADAL 307 E + L+ + G D + R+ + A + D R + A+V+A LA DAL Sbjct: 308 EAYAWHRALIAENAAGYDQRVVSRILRGKDMSAADLLDVLHARPRWIAQVEAQLAGYDAL 367 Query: 308 LLPTLPALPPTLQAIRQGTSVLLLSS---LVRP--FNLSGHPALTIPVPIAGSTLKAGLQ 362 ++PT+P + P + ++ ++ L P N AL++P GS GL Sbjct: 368 VMPTVPIVAPRIAELQASDDAYYGANGKMLRNPTLINFLDGCALSLPCHAPGSA-PVGLS 426 Query: 363 LVGRKGDDERLCALGRHLEQALQA 386 L G D+RL ++G +E L A Sbjct: 427 LAACGGQDKRLLSIGLAVEALLAA 450 >gi|300311728|ref|YP_003775820.1| indole acetamide hydrolase [Herbaspirillum seropedicae SmR1] Length = 468 Score = 109 bits (272), Expect = 5e-25, Method: Compositional matrix adjust. Identities = 72/156 (46%), Positives = 96/156 (61%), Gaps = 5/156 (3%) Query: 23 GGNGPT----VAIKDCIDIAGQPTCGGSAALADAPPAQSHAEVVQRLLDTGWQIIARANM 78 GG+GP +AIKD ID+AG + G+AAL + P Q HA VV+ L G +ANM Sbjct: 61 GGHGPLSGIPIAIKDNIDVAGIASTAGTAALRNNVP-QRHATVVEYLFGAGALHAGKANM 119 Query: 79 HELAYGMTGINDWNGTPVNPQDPGRMTGGSSSGSASAVGAGLVDLSIGSDTGGSIRLPAA 138 HELA+G+T N G NP D + GGSS G + + A L ++G+DTGGS+R+PAA Sbjct: 120 HELAFGITNNNGVFGPCRNPYDAELIAGGSSGGCGALLAARLAPAAVGTDTGGSVRIPAA 179 Query: 139 CCGVIGLKPTFGRVSRAGAYPAISSLDCVGPVARSM 174 CGV+GL+PT R S+ G P + D GP+ARS+ Sbjct: 180 LCGVVGLRPTSRRYSQHGVVPISHTRDTPGPMARSV 215 >gi|300309904|ref|YP_003773996.1| aspartate tRNAAsn/Glu-tRNAGln amidotransferase subunit A-like protein [Herbaspirillum seropedicae SmR1] Length = 651 Score = 95.5 bits (236), Expect = 7e-21, Method: Compositional matrix adjust. Identities = 66/151 (43%), Positives = 88/151 (58%), Gaps = 1/151 (0%) Query: 25 NGPTVAIKDCIDIAGQPTCGGSAALADAPPAQSHAEVVQRLLDTGWQIIARANMHELAYG 84 +G + +KD D PT GG+ ALA AQ+ A V+RL + G I+ + MHELA G Sbjct: 120 HGIPILVKDNYDTVDMPTTGGTLALATLQ-AQADAFQVKRLREAGAVILGKTTMHELAAG 178 Query: 85 MTGINDWNGTPVNPQDPGRMTGGSSSGSASAVGAGLVDLSIGSDTGGSIRLPAACCGVIG 144 +T ++ G NP DP R GGSS G+ +AV A +GSDT GSIR+PAA + G Sbjct: 179 VTTVSSLTGFTRNPYDPRRAPGGSSGGTGAAVAASFAAAGMGSDTCGSIRIPAAHQNLFG 238 Query: 145 LKPTFGRVSRAGAYPAISSLDCVGPVARSME 175 L+ T G SR+G P S+ D P+ARS+E Sbjct: 239 LRTTRGLASRSGVMPLSSTQDVAAPLARSVE 269 >gi|300310745|ref|YP_003774837.1| amidase family protein [Herbaspirillum seropedicae SmR1] Length = 611 Score = 85.9 bits (211), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 112/385 (29%), Positives = 164/385 (42%), Gaps = 36/385 (9%) Query: 26 GPTVAIKDCIDIAGQPTCGGSAALADAPPAQSHAEVVQRLLDTGWQIIARANMHELAYGM 85 G A+KD ID+AG PT + A++ A VVQRL G ++ + N+ + A G+ Sbjct: 85 GIPFAVKDNIDVAGMPTTAACPEFSRV--AETSATVVQRLQAAGALLVGKTNLDQFATGL 142 Query: 86 TGINDWNGTPVNPQDPGRMTGGSSSGSASAVGAGLVDLSIGSDTGGSIRLPAACCGVIGL 145 G+ G N P ++GGSSSGSA AV G V ++G+DT GS R+PAA ++GL Sbjct: 143 VGVRSPYGAVRNAIAPDYVSGGSSSGSAVAVALGQVMFALGTDTAGSGRIPAAFNHLVGL 202 Query: 146 KPTFGRVSRAGAYPAISSLDCVGPVARSMEMIIRAMTSIAPGFDRVAASAAAAHARVKRV 205 KP+ G +S G PA +LDC+ A + + S A A + +KR Sbjct: 203 KPSRGLISAQGLVPACRTLDCISIFAHDVADAWHVLHSAAGYDAADAYARKLPMLGLKRR 262 Query: 206 TVQADDDVAAAVAQAFSASRWSATWWTPQHMHAAFEAGMTLINRETHDAF---GHLLGQG 262 + +A A F + W A G+TL R F LL QG Sbjct: 263 GYR----IAIPAATEFYGDLAAQDAWDRTLARIAELPGVTL-ARIPFAPFADAARLLYQG 317 Query: 263 KLGAD------------------IEQRLAAAATTTDAAVADAERVR-AAFSAEVDAALAD 303 A+ + + + A A DA A + R A + + L Sbjct: 318 PWVAERRAALGDFFVKQPQAVHPVVREITAQAADYDAVAAFQGQYRLAELRRQAEQLLEG 377 Query: 304 ADALLLPTLPALPPTLQA----IRQGTSVLLLSSLVRPFNLSGHPALTIPVPIAGSTLKA 359 AD +L+PT +P + +R+ + + ++ V NL AL IP L A Sbjct: 378 ADLMLVPTTTGMPTIAEVQADPVRRNSQLGYYTNFV---NLMDMSALAIPGLWRNDGLPA 434 Query: 360 GLQLVGRKGDDERLCALGRHLEQAL 384 G+ L+G G D L G L+ L Sbjct: 435 GVTLIGPAGADHLLAEAGARLQHQL 459 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.131 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,059,884 Number of extensions: 39674 Number of successful extensions: 181 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 172 Number of HSP's successfully gapped: 11 Length of query: 391 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 304 Effective length of database: 1,207,925 Effective search space: 367209200 Effective search space used: 367209200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)