BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1142.p (318 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310671|ref|YP_003774763.1| flavodoxin reductases (ferredox... 573 e-165 gi|300313278|ref|YP_003777370.1| vanillate O-demethylase (vanill... 253 1e-68 gi|300309798|ref|YP_003773890.1| flavodoxin reductases (ferredox... 210 1e-55 gi|300311823|ref|YP_003775915.1| nitric oxide dioxygenase [Herba... 66 3e-12 >gi|300310671|ref|YP_003774763.1| flavodoxin reductases (ferredoxin-NADPH reductases) family 1 protein [Herbaspirillum seropedicae SmR1] Length = 318 Score = 573 bits (1478), Expect = e-165, Method: Compositional matrix adjust. Identities = 291/318 (91%), Positives = 298/318 (93%) Query: 1 MKLIVDRLTDETGDIRSFRLVRADGAALPAYEPGAHIDVTGPTGIVRQYSLCGPLEDRCG 60 MKLIVD LTDETGDIRSFRLVRADGA LPAYEPGAHIDVTGPTGI+RQYSLCGPLEDRCG Sbjct: 1 MKLIVDTLTDETGDIRSFRLVRADGAPLPAYEPGAHIDVTGPTGIMRQYSLCGPLEDRCG 60 Query: 61 YTIAVKREPASRGGSAALHDTVQLGMELEVGTPRNLFALDARAPEHLLFGAGIGVTPLLA 120 YTIAVKRE ASRGGSAALHD V+ GMELEVGTPRNLFALDA APEHLLFGAGIGVTPLLA Sbjct: 61 YTIAVKRESASRGGSAALHDVVKAGMELEVGTPRNLFALDASAPEHLLFGAGIGVTPLLA 120 Query: 121 MAYRLWAEQKPFTLHYFVRAREHAAFVDLLENAPFAAQVCFHYAVMPAQMEHYLAACLAA 180 MAYRLWAE+K FTLHYFVRAREHAAF DLLENAPFAA V FHYAVMPAQMEHYL CL A Sbjct: 121 MAYRLWAEEKRFTLHYFVRAREHAAFADLLENAPFAAHVRFHYAVMPAQMEHYLTVCLEA 180 Query: 181 GSKEAHVYTCGPAPFMEAVVKCAAANRSEEAIHLEHFQAAPAASEAVGGDGVFEVQLASS 240 GSKEAHVYTCGPAPFM+AVVKCA+A RSEEAIHLEHFQAAPAA A GGDG FEV+LASS Sbjct: 181 GSKEAHVYTCGPAPFMDAVVKCASATRSEEAIHLEHFQAAPAAPAAAGGDGAFEVKLASS 240 Query: 241 GKVVQIPVGIPIVDVLAREGIVIDTSCREGICGTCVVPLLEGEPDHRDNCLSKKEKAAND 300 GKVVQIPVGIPIVD+LAREGIVIDTSCREGICGTCVVPLLEGEPDHRDNCLSKKEKAAND Sbjct: 241 GKVVQIPVGIPIVDILAREGIVIDTSCREGICGTCVVPLLEGEPDHRDNCLSKKEKAAND 300 Query: 301 QICTCVSRARSARLVLDL 318 QICTCVSRARS LVLDL Sbjct: 301 QICTCVSRARSGMLVLDL 318 >gi|300313278|ref|YP_003777370.1| vanillate O-demethylase (vanillate degradation ferredoxin-like) oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 318 Score = 253 bits (647), Expect = 1e-68, Method: Compositional matrix adjust. Identities = 149/310 (48%), Positives = 188/310 (60%), Gaps = 5/310 (1%) Query: 10 DETGDIRSFRLVRADGAALPAYEPGAHIDVTGPTGIVRQYSLCGPLEDRCGYTIAVKREP 69 +E DI + LV DGAALPA+ GAHIDV P G+VRQYSLC ++ Y I V R+ Sbjct: 13 EEAHDIIALELVAVDGAALPAFTAGAHIDVHLPGGLVRQYSLCNAPHEQHRYQIGVLRDV 72 Query: 70 ASRGGSAALHDTVQLGMELEVGTPRNLFALDARAPEHLLFGAGIGVTPLLAMAYRLWAEQ 129 SRGGS A+H+ ++LG L + PRN FAL A LL GIGVTP+L MA L A Sbjct: 73 NSRGGSVAVHEALKLGDILTISAPRNQFAL-VPAQHSLLLAGGIGVTPILCMAEALAAAG 131 Query: 130 KPFTLHYFVRAREHAAFVDLLENAPFAAQVCFHYAVMPAQMEHYLAACLAAGSKEAHVYT 189 F +HY R+ AF + + + FAA+V HY A + L A LA H+Y Sbjct: 132 ASFEMHYCARSPARQAFRERIAASGFAARVVHHYDDGEAAQKLDLDALLAQADPATHLYV 191 Query: 190 CGPAPFMEAVVKCAAANRSEEA-IHLEHFQAAPAASEAVGGDGVFEVQLASSGKVVQIPV 248 CGPA F+ VV A A EA +H E+F AAP ++ G+G FEV+LASSG+V IP Sbjct: 192 CGPAGFIAHVVDTARARGWPEAQVHFEYFGAAPVKAD---GNGAFEVKLASSGQVYTIPP 248 Query: 249 GIPIVDVLAREGIVIDTSCREGICGTCVVPLLEGEPDHRDNCLSKKEKAANDQICTCVSR 308 ++ VL G+ + SC +GICGTC+ +LEGEPDHRD L+ +E+AANDQ C SR Sbjct: 249 ERTVIQVLCEHGVDVPVSCEQGICGTCLTRVLEGEPDHRDQYLTDEERAANDQFTPCCSR 308 Query: 309 ARSARLVLDL 318 ARSA LVLDL Sbjct: 309 ARSALLVLDL 318 >gi|300309798|ref|YP_003773890.1| flavodoxin reductases (ferredoxin-NADPH reductases) family 1 protein [Herbaspirillum seropedicae SmR1] Length = 339 Score = 210 bits (534), Expect = 1e-55, Method: Compositional matrix adjust. Identities = 144/328 (43%), Positives = 182/328 (55%), Gaps = 14/328 (4%) Query: 1 MKLIVDRLTDETGDIRSFRLVRADGAALPAYEPGAHIDVTGPTGIVRQYSLCGPLEDRCG 60 + L V + DIR LV DGAALP + PGAH+ + G+VR YSLC + R Sbjct: 16 LMLRVRAVRQAAQDIRLLELVSQDGAALPPWTPGAHLRLQLQDGLVRAYSLCNTPQQRDC 75 Query: 61 YTIAVKREPASRGGSAALHDTVQLGMELEVGTPRNLFAL--DARAPEHLLFGAGIGVTPL 118 Y IAVKREP SRGGSA LH+ VQ G + P N F L D RAP LL AGIG+TPL Sbjct: 76 YLIAVKREPDSRGGSAFLHEQVQEGTTISASPPVNAFPLIADVRAP--LLLAAGIGITPL 133 Query: 119 LAMAYRLWAEQKPFTLHYFVRAREHAAFVDLLENAPFAAQVCFHYAVMPAQMEHYLAACL 178 AMA L + + LHYF R+ HAAF E A + + H + A+ EH +AA L Sbjct: 134 HAMAAELATQGRRHRLHYFARSLAHAAFFH--ELAAGSQGMSLHLGLDGAETEHAIAAAL 191 Query: 179 AAGSKEAHVYTCGPAPFMEAVVKCAA-ANRSEEAIHLEHFQAAPAASEAV-------GGD 230 AA + + +Y CGP+PF++A + A A E+ IH E F A + D Sbjct: 192 AAAPRSSPLYVCGPSPFIDAARRHAQLAGWREQDIHFERFAAPAMPAAEADPARPVQAPD 251 Query: 231 GVFEVQLASSGKVVQIPVGIPIVDVLAREGIVIDTSCREGICGTCVVPLLEGEPDHRDNC 290 FE+ L SG Q+ G IV A+ G+VI TSC EG CG+C +LEG+P HRD+ Sbjct: 252 STFELVLQRSGLRCQVLPGQSIVAAAAQVGVVIGTSCGEGFCGSCESTVLEGQPWHRDSV 311 Query: 291 LSKKEKAANDQICTCVSRARSARLVLDL 318 LS E+A+ +I CVSR RLVLDL Sbjct: 312 LSAAERASGRRILPCVSRCAGTRLVLDL 339 >gi|300311823|ref|YP_003775915.1| nitric oxide dioxygenase [Herbaspirillum seropedicae SmR1] Length = 397 Score = 66.2 bits (160), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 66/223 (29%), Positives = 92/223 (41%), Gaps = 23/223 (10%) Query: 2 KLIVDRLTDETGDIRSFRLVRADGAALPAYEPGAHID---VTGPTGIVRQYSLCGPLEDR 58 IV R E+ +I SF V DG L + PG +I V RQYSL L Sbjct: 157 NFIVSRKVAESEEITSFHFVPQDGGELLDFAPGQYIGLRMVVDGEEQRRQYSLSALLRQA 216 Query: 59 CG---YTIAVKREPASRGGSAALHDTVQLGMELEVGTPRNLFALDARAPEHLLFGAGIGV 115 Y I+VKREP + S LHD + +G +E+ P F L A L G+G+ Sbjct: 217 GQPPQYRISVKREPGGKV-SRQLHDQIHVGSVVELFPPAGEFTLMASDKPLALISGGVGI 275 Query: 116 TPLLAMAYRLWAEQKPFTLHYFVRARE---HAAFVDLLENAPFAAQVCFHYAV-MPAQ-- 169 TP+++M +P +H+ AR HA L E A Q+ + P Q Sbjct: 276 TPMMSMLAAALPSGRP--IHFIHAARHGGVHAFRRQLEELAARHPQLQLRFCYEQPRQGD 333 Query: 170 --------MEHYLAACLAAGSKEAHVYTCGPAPFMEAVVKCAA 204 +E L G+ + Y GP FM+A+ + A Sbjct: 334 AAPHATGLIERSLLQQWLPGNPDLDAYFVGPKSFMKAIKRQLA 376 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.137 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,047,970 Number of extensions: 45975 Number of successful extensions: 143 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 137 Number of HSP's successfully gapped: 4 Length of query: 318 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 233 Effective length of database: 1,217,395 Effective search space: 283653035 Effective search space used: 283653035 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)