BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1129.p (262 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310643|ref|YP_003774735.1| cis-diol dehydrogenase [Herbasp... 432 e-122 gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herb... 100 2e-22 gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reduc... 90 2e-19 gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein... 87 1e-18 gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum s... 86 4e-18 >gi|300310643|ref|YP_003774735.1| cis-diol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 257 Score = 432 bits (1112), Expect = e-122, Method: Compositional matrix adjust. Identities = 213/257 (82%), Positives = 232/257 (90%) Query: 6 VRARFAGKVAVVTGAAQGILRGVALGIAAEGGQVLLVDRADFIDEVAAQAEAGRGSGFVA 65 +++RFAGKV VVTGAAQGILRGVALGIA EGGQVLLVDRA+F+ +VAA+A GRG G VA Sbjct: 1 MKSRFAGKVVVVTGAAQGILRGVALGIAEEGGQVLLVDRAEFVHDVAAEAAGGRGKGLVA 60 Query: 66 DLETYAGAQAAMAQAIARYGRIDILINGVGGAIRMRPYDQFEAAQIDAEIRRSLMPTLYG 125 DLE+YAGAQAAM +A+ RYGRIDILINGVGGAIRMRPY Q+ QIDAEIRRSLMPTLYG Sbjct: 61 DLESYAGAQAAMDEAVQRYGRIDILINGVGGAIRMRPYAQYTPTQIDAEIRRSLMPTLYG 120 Query: 126 CHAVLPYLLRQGSGTIVNVSSNATRGIRRVPYSAAKGGVNAMTTALAMEYGEQGIRVVAT 185 CHAVLP LL+QG G IVNVSSNATRGIRRVPYSAAKGGVNA+T ALAMEYGE GIRVVAT Sbjct: 121 CHAVLPQLLQQGRGVIVNVSSNATRGIRRVPYSAAKGGVNALTAALAMEYGELGIRVVAT 180 Query: 186 APGGTEAPPRRVPRNADGDSAQEQAWMQQAVQQVTSSTFFKRYATLEEQIAPILFLASDE 245 APGGT+APPRRVPRN +GDS QEQ WMQ+AVQQVT S +FKRY+TLEEQIAPILFLASD+ Sbjct: 181 APGGTQAPPRRVPRNPEGDSQQEQMWMQEAVQQVTGSNYFKRYSTLEEQIAPILFLASDD 240 Query: 246 ASYITGTVLPVAAGDNG 262 ASYITG VLPVA GDNG Sbjct: 241 ASYITGAVLPVAGGDNG 257 >gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 100 bits (248), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 86/256 (33%), Positives = 123/256 (48%), Gaps = 21/256 (8%) Query: 12 GKVAVVTGAAQGILRGVALGIAAEGGQVLLV-DRADFIDEVAA--QAEAGRGSGFVADLE 68 GKVA++TGA+ GI R AL A G ++L R D +++VAA A+ GR S D+ Sbjct: 6 GKVALITGASAGIGRATALLFARHGAALILTARRQDALEQVAALICAQGGRASVVAGDVG 65 Query: 69 TYAGAQAAMAQAIARYGRIDILINGVGGAIRMRPYDQFEAAQIDAEIRRSLMPTLYGCHA 128 A + +A A+ +G +DI IN G +P + Q + +L G Sbjct: 66 EAATHERGVAMALDEFGGLDIAINNAGAVGACKPLAEISPQQWQDTLHANLTSAFLGARC 125 Query: 129 VLPYLLRQGSGTIVNVSS--NATRGIRRV-PYSAAKGGVNAMTTALAMEYGEQGIRVVAT 185 +P +L +G+G IV SS ++ G+ + Y AAK G+ + +A +Y QGIR A Sbjct: 126 QIPAMLARGAGAIVFTSSFVGSSAGLPGMAAYGAAKAGLMGLVKGIAADYAVQGIRANAL 185 Query: 186 APGGTEAPPRRVPRNADGDSAQEQAWMQQAVQQVTSSTFFKRYATLEEQIAPILFLASDE 245 PGG V + GD Q+Q W KR A EE A LFLAS Sbjct: 186 LPGG-------VDTDMGGDPQQKQ-W-------AAGLHAMKRIAQPEEIAAAALFLASPM 230 Query: 246 ASYITGTVLPVAAGDN 261 AS++TGT L G++ Sbjct: 231 ASFVTGTALYADGGNS 246 >gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 261 Score = 89.7 bits (221), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 80/271 (29%), Positives = 125/271 (46%), Gaps = 38/271 (14%) Query: 3 GPAVRARFAGKVAVVTGAAQGILRGVALGIAAEGGQVLLVDR-----ADFIDEVAAQAEA 57 P+ RF GKV +VTGAA GI A + EG +VLL DR D + + A Sbjct: 5 NPSRLQRFEGKVVIVTGAASGIGEATARRFSDEGARVLLADRDAAALGKVFDSLPPERTA 64 Query: 58 GRGSGFVADLETYAGAQAAMAQAIARYGRIDILIN--GV---GGAIRMRPYD--QFEAAQ 110 R + D+ + + + AI R+G++D+L++ GV G + P D + +A Sbjct: 65 ARET----DVSHHEQVRQLVDFAIERFGQLDVLVSDAGVFAEGNVTEVSPEDWHRVQATN 120 Query: 111 IDAEIRRSLMPTLYGCHAVLPYLLRQGSGTIVNVSSNA--TRGIRRVPYSAAKGGVNAMT 168 ++ YG LP+ L + G IVNV+S + Y+A+KG V +T Sbjct: 121 VNG--------VFYGAREALPH-LEKTRGCIVNVASVSGLAADWNLSAYNASKGAVCNLT 171 Query: 169 TALAMEYGEQGIRVVATAPGGTEAPPRRVPRNADGDSAQEQAWMQQAVQQVTSSTFFKRY 228 A+A+++G +G+R+ A P T D A + + + +++ R Sbjct: 172 RAMALDFGRKGVRINAVCPSLTHTA-------MTADMADDPPLLDKFAERIA----LGRG 220 Query: 229 ATLEEQIAPILFLASDEASYITGTVLPVAAG 259 A E A I FLAS +AS++ G LPV G Sbjct: 221 ADPLEIAAVITFLASPDASFVNGVNLPVDGG 251 >gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 87.4 bits (215), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 88/255 (34%), Positives = 125/255 (49%), Gaps = 23/255 (9%) Query: 13 KVAVVTGAA--QGILRGVALGIAAEGGQVLLVDRADFIDEVAAQAEAGRGSGFVADLETY 70 KVA++TG A G+ A +AA+G +++++D AA + G VAD+ Sbjct: 6 KVAIITGGAGQNGLGFSTARLMAAQGARIVILDLERAEPAGAAARLGAQHLGLVADVTDK 65 Query: 71 AGAQAAMAQAIARYGRIDILINGVGGAIRMRPYDQFEAAQIDAE--IRRSLMPTLYGCHA 128 A +AA A + ++GRIDIL+N G +P E D + + SL TLY A Sbjct: 66 AACEAAAAAVLKQFGRIDILVNNAG---VTQPAKLLEITGKDYDRILDVSLRGTLYMSQA 122 Query: 129 VLPYLLRQGSGTIVNVSSNATR---GIRRVP-YSAAKGGVNAMTTALAMEYGEQGIRVVA 184 VLP + Q SG+IV +SS + + GI P YSAAK GV + A+A E+G QGIRV Sbjct: 123 VLPAMQAQQSGSIVCISSVSAQRGGGILGGPHYSAAKAGVLGLARAMAREFGPQGIRVNC 182 Query: 185 TAPGGTEAPPRRVPRNADGDSAQEQAWMQQAVQQVTSSTFFKRYATLEEQIAPILFLASD 244 PG N D SA+++A + Q + R ++ +FLASD Sbjct: 183 ITPGLISTDI-----NKDILSAEKKAEIAQTIP-------LGRLGGPDDVGGVCVFLASD 230 Query: 245 EASYITGTVLPVAAG 259 + Y TG L V G Sbjct: 231 LSQYCTGVTLDVNGG 245 >gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 85.5 bits (210), Expect = 4e-18, Method: Compositional matrix adjust. Identities = 82/257 (31%), Positives = 123/257 (47%), Gaps = 27/257 (10%) Query: 13 KVAVVTGAA--QGILRGVALGIAAEGGQVLLVDRADFIDEVAAQAEAGRGSGFVADLETY 70 KVAV+TG A G+ A +A +G +V+++D A AA + G VAD+ Sbjct: 6 KVAVITGGAGINGLGYATARLMAEQGARVVVLDLAQADPAAAAARIGSQHLGLVADVTDK 65 Query: 71 AGAQAAMAQAIARYGRIDILINGVGGAIRMRPYDQFE--AAQIDAEIRRSLMPTLYGCHA 128 A + A A + ++GRIDIL+N G +P E A D + SL TLY A Sbjct: 66 ASCETAAAAILQQFGRIDILVNNAG---ITQPVKTLEITGADYDRILDVSLRGTLYMSQA 122 Query: 129 VLPYLLRQGSGTIVNVSSNATR---GIRRVP-YSAAKGGVNAMTTALAMEYGEQGIRVVA 184 VLP + Q +G+I+ +SS + + GI P YSAAK GV + A+A E+G +GIRV + Sbjct: 123 VLPAMRAQQNGSIICISSVSAQRGGGILGGPHYSAAKAGVLGLARAMAREFGPEGIRVNS 182 Query: 185 TAPG--GTEAPPRRVPRNADGDSAQEQAWMQQAVQQVTSSTFFKRYATLEEQIAPILFLA 242 PG GT+ ++ ++ ++ + R ++ +FL Sbjct: 183 ITPGLIGTDIIKGKLT--------------EEKKAEIAETIPLARLGRADDIGGACVFLG 228 Query: 243 SDEASYITGTVLPVAAG 259 SD + Y TG L V G Sbjct: 229 SDLSQYCTGITLDVNGG 245 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 762,350 Number of extensions: 28605 Number of successful extensions: 269 Number of sequences better than 1.0e-08: 41 Number of HSP's gapped: 201 Number of HSP's successfully gapped: 41 Length of query: 262 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 179 Effective length of database: 1,226,865 Effective search space: 219608835 Effective search space used: 219608835 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)