BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1126.p (457 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310640|ref|YP_003774732.1| achlorobenzoate 1,2-dioxygenase... 894 0.0 gi|300310673|ref|YP_003774765.1| phenylpropionate dioxygenase la... 194 1e-50 gi|300312958|ref|YP_003777050.1| anthranilate dioxygenase large ... 132 5e-32 gi|300313128|ref|YP_003777220.1| dioxygenase subunit alpha oxido... 73 5e-14 >gi|300310640|ref|YP_003774732.1| achlorobenzoate 1,2-dioxygenase subunit alpha [Herbaspirillum seropedicae SmR1] Length = 462 Score = 894 bits (2309), Expect = 0.0, Method: Compositional matrix adjust. Identities = 420/449 (93%), Positives = 436/449 (97%), Gaps = 1/449 (0%) Query: 1 MNTIAA-DKYQDVERLLESALDEDATNGIFRCRRDIFTNEALFELEMKHLFESNWVYLAH 59 MNT AA D+Y +++ LL+SAL ED NG+FRCRRDIFTN LFELEMKHLFESNWVYLAH Sbjct: 1 MNTTAAADRYDELDTLLQSALQEDKDNGVFRCRRDIFTNPDLFELEMKHLFESNWVYLAH 60 Query: 60 ESQIPEINDYYTTWIGRQPVVVTRDKTGELHAVINACAHKGAMLCRRKHGNKSSFTCPFH 119 ESQIPEINDYYTTWIGRQPVV+TRDK+G+L+AVINAC+HKGAMLCRRKHGNKSSFTCPFH Sbjct: 61 ESQIPEINDYYTTWIGRQPVVITRDKSGQLNAVINACSHKGAMLCRRKHGNKSSFTCPFH 120 Query: 120 GWTFSNSGKLLKVKDEKTTEYPVSFNQNGSHDLTRVARFQSYRGFLFGSLSEDVLPLEEY 179 GWTFSNSGKLLKVKDEKTTEYPVSFN+NGSHDLTRVARFQSYRGFLFGSLSEDVLPLEEY Sbjct: 121 GWTFSNSGKLLKVKDEKTTEYPVSFNKNGSHDLTRVARFQSYRGFLFGSLSEDVLPLEEY 180 Query: 180 LGETRVIIDQIVDQAPNGLEVLRGNSSYLYDGNWKMQMENGCDGYHVSSVHWNYAATMGR 239 LGETRVIIDQIVDQAPNGLEVLRGNSSYLYDGNWKMQMENGCDGYHVSSVHWNYAATM R Sbjct: 181 LGETRVIIDQIVDQAPNGLEVLRGNSSYLYDGNWKMQMENGCDGYHVSSVHWNYAATMSR 240 Query: 240 RKEGGTKATDANNWSKAVAGVYGFENGHILLWTNTLNPEVRPIWNQREELAARVGQARAD 299 RKEGGTKATDANNWSKAVAGVYGFENGHILLWTNTLNPEVRPIWNQREELAARVGQARAD Sbjct: 241 RKEGGTKATDANNWSKAVAGVYGFENGHILLWTNTLNPEVRPIWNQREELAARVGQARAD 300 Query: 300 TIVAQTRNLCLYPNVFLMDQFGTQIRVARPISVDKTEISIFCFGPKGESAEDRRVRIRQY 359 TIVAQTRNLCLYPNVFLMDQFGTQIRVARPISVDKTEISIFCF PKGES EDRRVRIRQY Sbjct: 301 TIVAQTRNLCLYPNVFLMDQFGTQIRVARPISVDKTEISIFCFAPKGESEEDRRVRIRQY 360 Query: 360 EDFFNVSGMGTADDLEEFRACQAGYAGSAVRWNDMSRGAPLWKQGPDENAKKMGMNPILS 419 EDFFNVSGMGTADDLEEFRACQAGYAGSAVRWNDMSRGAPLWK+GPDENA+KMGM+P LS Sbjct: 361 EDFFNVSGMGTADDLEEFRACQAGYAGSAVRWNDMSRGAPLWKEGPDENARKMGMHPKLS 420 Query: 420 GERSEDEGLFVCQHHYWTEQMRQAVKKER 448 GERSEDEGLFVCQHHYWTEQMR+AVKKER Sbjct: 421 GERSEDEGLFVCQHHYWTEQMRKAVKKER 449 >gi|300310673|ref|YP_003774765.1| phenylpropionate dioxygenase large terminal subunit [Herbaspirillum seropedicae SmR1] Length = 428 Score = 194 bits (494), Expect = 1e-50, Method: Compositional matrix adjust. Identities = 120/371 (32%), Positives = 188/371 (50%), Gaps = 42/371 (11%) Query: 35 IFTNEALFELEMKHLFESNWVYLAHESQIPEINDYYTTWIGRQPVVVTRDKTGELHAVIN 94 ++T+ ALFE EM +F W+++AHES+I D+ T IGRQPV+V RDKTG+++ + N Sbjct: 29 LYTDPALFEAEMDKIFYKTWIWVAHESEIKNPGDFKTAQIGRQPVIVVRDKTGKINVLEN 88 Query: 95 ACAHKGAMLCRRKHGNKSSFTCPFHGWTFSNSGKLLKVKDEKTTEYPVSFNQNGSHDLTR 154 C H+GA +C + GN + FTCP+H W++ GKL + YP G D+ Sbjct: 89 RCRHRGATVCEKHKGNATGFTCPYHSWSYGLDGKL------RGLPYP-----EGYEDVIE 137 Query: 155 VA-------RFQSYRGFLFGSLSEDVLPLEEYLGETRVIIDQIVDQAPNGLEVLRGNSSY 207 A R +SY G +F S ++++ PL ++LG + ID + Q ++G + Sbjct: 138 KADLPLQSLRCESYHGMIFASFNQEIEPLSDFLGHAKKWIDLFMKQGAGFPIKVQGEHKF 197 Query: 208 LYDGNWKMQMENGCDGYHVSSVHWNYAATMGRR-KEGGTKATDANNWSKAVAGVYGFENG 266 + GNWK+Q+EN DGYH VH + +++ E + TD + ++++ NG Sbjct: 198 SFKGNWKIQLENTTDGYHFPVVHKTFLSSVDEETSEMLSFMTDDQSITRSLG------NG 251 Query: 267 H--ILLWTNTLNPEVRPIWNQREELAARVGQARADTIVA-QTR-----------NLCLYP 312 H +++ ++ +V Q +E A V + + T+ Q R NL L+P Sbjct: 252 HSVMIMVPEHVDLDVDDGSEQLQERFAHVTEELSKTMPPEQVRRIVRSLHGAGFNLNLFP 311 Query: 313 NVFLMDQFGTQIRVARPISVDKTEISIFCFGPKGESAEDRRVRIRQYEDFFNVSGMGTAD 372 NV + F RV P+SV +T+I G G R R+R +E F G G+ D Sbjct: 312 NVAMSMSF---FRVLHPVSVGETQIRHVALGMDGGPEIANRERLRIHEHFQGPFGFGSPD 368 Query: 373 DLEEFRACQAG 383 D E + QAG Sbjct: 369 DAEAWERVQAG 379 >gi|300312958|ref|YP_003777050.1| anthranilate dioxygenase large subunit [Herbaspirillum seropedicae SmR1] Length = 426 Score = 132 bits (333), Expect = 5e-32, Method: Compositional matrix adjust. Identities = 112/394 (28%), Positives = 172/394 (43%), Gaps = 47/394 (11%) Query: 30 RCRRDIFTNEALFELEMKHLFE-SNWVYLAHESQIPEINDYYTTWIGRQPVVVTRDKTGE 88 R +F++ +++LE + ++ W +LA E++IP DY +T++G PVVVTR + G Sbjct: 25 RTPYKVFSSREVYDLEQERIYRGETWSFLALEAEIPNNGDYKSTFVGDTPVVVTRTEDGA 84 Query: 89 LHAVINACAHKGAMLCRRKHGNKSSFTCPFHGWTFSNSGKLLKVKDEKTTEYPVSFNQN- 147 L A +N C+H+GAM+CR GN SS +C +H W+F + G LL V + + ++ Sbjct: 85 LAAWVNKCSHRGAMVCRTPRGNASSHSCAYHQWSFDSRGNLLGVPFRRGQKGATGMAKDF 144 Query: 148 --GSHDLTRVARFQSYRGFLFGSLSEDVLPLEEYLG-ETRVIIDQIVDQAPNGLEVLRGN 204 H L R R SY+G +F + SE V L +Y+G E R ID+I + L R Sbjct: 145 DPKCHGL-RQLRVDSYKGLVFATFSETVEALPDYIGAEMRPWIDRIFHKPIVYLGCTRQY 203 Query: 205 SSYLYDGNWKMQMENGCDGYHVSSVHWNY-----------AATMGRRKEGGTKATDANNW 253 S NWK+ +EN D YH S +H + A ++ + G A Sbjct: 204 SK----SNWKLYLENVKDPYHASLLHLFHTTFNIFRVGMKARSLADKHHGLHSIITATKN 259 Query: 254 SKAVAGVY------GFENGHILLWTNTLNPEVRPIWNQREELAARVGQARADTIVAQTRN 307 A Y F+ G L +P V + EEL Q +V Q + Sbjct: 260 EVETADAYKQQEIRSFDEGFSL-----QDPSVLGQIKEFEELTTNHIQPIFPQLVVQQIH 314 Query: 308 LCLYPNVFLMDQFGTQIRVARPISVDKTEISIFCFGPKGESAEDRRVRIRQYEDFFNVSG 367 L L P E+ FG + ++ E R +RI+Q + +G Sbjct: 315 NTLVARQLL------------PKGPSNFELVFHFFGYEDDTPELRALRIKQ-ANLVGPAG 361 Query: 368 MGTADDLEEFRACQAGYAGSAVRWN--DMSRGAP 399 + +D E Q G A + + DM+R P Sbjct: 362 YISMEDTEATELVQRGTARDPDQCSVIDMARDNP 395 >gi|300313128|ref|YP_003777220.1| dioxygenase subunit alpha oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 374 Score = 72.8 bits (177), Expect = 5e-14, Method: Compositional matrix adjust. Identities = 56/204 (27%), Positives = 88/204 (43%), Gaps = 36/204 (17%) Query: 35 IFTNEALFELEMKHLFESNWVYLAHESQIPEINDYYTTWIGRQPVVVTRDKTGELHAVIN 94 ++ +E L++ E+K LF+ Y+ HE +PE DY T + ++ R+ G + V N Sbjct: 21 VYFDEPLYQRELKQLFQQGPRYVGHELMVPEAGDYSTLPWENEGRMLVRNAQG-IELVSN 79 Query: 95 ACAHKGAMLCRRKHGNKSSFTCPFHGWTFSNSGKLLKVKDEKTTEYPVSFNQNGSHDLTR 154 C H+ A + + G+ S+ CP H WT+ G+L+ F Q +L++ Sbjct: 80 VCRHRQAKMLDGR-GHASNIVCPLHRWTYDLKGELIGAP---------HFGQTPCMNLSK 129 Query: 155 VARFQSYRGFLFGSLSEDVLPLEEYLGETRVIIDQIVDQAPNGLEVLRGNSSYLYDG--- 211 QS+ G LF + D+ + LG TR + S YLYD Sbjct: 130 TP-LQSWNGLLFEANGVDIADKLKNLGVTRDL----------------DFSGYLYDHTEV 172 Query: 212 -----NWKMQMENGCDGYHVSSVH 230 NWK +E + YHV H Sbjct: 173 HECDYNWKTFIEVYLEDYHVEPFH 196 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,473,873 Number of extensions: 62725 Number of successful extensions: 135 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 130 Number of HSP's successfully gapped: 4 Length of query: 457 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 369 Effective length of database: 1,203,190 Effective search space: 443977110 Effective search space used: 443977110 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)