BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1125.p (311 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310639|ref|YP_003774731.1| catechol 1,2-dioxygenase [Herba... 608 e-175 gi|300312960|ref|YP_003777052.1| catechol 1,2-dioxygenase [Herba... 316 2e-87 gi|300313016|ref|YP_003777108.1| 6-chlorohydroxyquinol-1,2-dioxy... 124 1e-29 gi|300313273|ref|YP_003777365.1| protocatechuate 3,4-dioxygenase... 60 2e-10 >gi|300310639|ref|YP_003774731.1| catechol 1,2-dioxygenase [Herbaspirillum seropedicae SmR1] Length = 311 Score = 608 bits (1567), Expect = e-175, Method: Compositional matrix adjust. Identities = 295/311 (94%), Positives = 300/311 (96%) Query: 1 MAAKIFSTPEVQDFLRLASGLDTEGGNPRVKQIVHRILSDLFKTIEDLEITSDEYWAGVA 60 MAAKIFST EVQDFLRLASGLDTEGGNPR KQIVHRILSDLFKTIEDLEITSDEYWAGVA Sbjct: 1 MAAKIFSTREVQDFLRLASGLDTEGGNPRTKQIVHRILSDLFKTIEDLEITSDEYWAGVA 60 Query: 61 YINRLGTASEGGLLSPGLGLDRFLDMRMDAEDAAQGITGGTPRTIEGPLYVAGAPVEQGF 120 YINRLGTA E GLLSPGLGLDRFLDMRMDAEDAA GI GGTPRTIEGPLYVAGAPVEQGF Sbjct: 61 YINRLGTAHEAGLLSPGLGLDRFLDMRMDAEDAALGIAGGTPRTIEGPLYVAGAPVEQGF 120 Query: 121 ARLDDGSDKNGHTIIMHGTIYGADGKPMPGAQVEVWHANTKGFYSHFDPTGEQKPFNMRR 180 ARLDDGSDK+G TIIMHGT+YGADGKPMPGAQVEVWHANTKGFYSHFDPTGEQKPFNMRR Sbjct: 121 ARLDDGSDKSGQTIIMHGTVYGADGKPMPGAQVEVWHANTKGFYSHFDPTGEQKPFNMRR 180 Query: 181 TIITDAQGRYKFRSIMPAAYGCPPAGPTQALLNQLGRHGNRPAHIHFFVTADGHRKLTTQ 240 TIITDAQGRYKFRSIMP AYGCPPAGPTQ LLN LGRHGNRPAHIHFFVTA+GHRKLTTQ Sbjct: 181 TIITDAQGRYKFRSIMPTAYGCPPAGPTQGLLNALGRHGNRPAHIHFFVTAEGHRKLTTQ 240 Query: 241 INIDGDPLIYDDFAYATREGLVPPLVERTDAASIKAEGLEGPFAEIVFDMTLTSLVNGAD 300 INI+GDPLIYDDFAYATREGLVPPLVERTDAASIKAEGLEGPFAEIVFD+ LTSLVNGAD Sbjct: 241 INIEGDPLIYDDFAYATREGLVPPLVERTDAASIKAEGLEGPFAEIVFDIKLTSLVNGAD 300 Query: 301 NQIVDRPRLAA 311 NQIVDRPRLAA Sbjct: 301 NQIVDRPRLAA 311 >gi|300312960|ref|YP_003777052.1| catechol 1,2-dioxygenase [Herbaspirillum seropedicae SmR1] Length = 303 Score = 316 bits (809), Expect = 2e-87, Method: Compositional matrix adjust. Identities = 154/286 (53%), Positives = 199/286 (69%), Gaps = 3/286 (1%) Query: 26 GNPRVKQIVHRILSDLFKTIEDLEITSDEYWAGVAYINRLGTASEGGLLSPGLGLDRFLD 85 GN RVK +V+R++ D+F TIE+L++ +E+WA V Y+ G + E GL++ GLG + FLD Sbjct: 21 GNQRVKTVVNRLVRDMFYTIEELDVQPEEFWAAVDYLTSAGKSGEFGLIAAGLGFEHFLD 80 Query: 86 MRMDAEDAAQGITGGTPRTIEGPLYVAGAPVEQGFARLDDGSDKNGHTIIMHGTIYGADG 145 +RMD +A +G+ GGTPRTIEGPLYV+GAP ++GFAR+D + G T+ M G ++ G Sbjct: 81 LRMDEAEARRGVQGGTPRTIEGPLYVSGAPEQKGFARMDKDP-QPGDTLFMQGQVFDEHG 139 Query: 146 KPMPGAQVEVWHANTKGFYSHFDPTGEQKPFNMRRTIITDAQGRYKFRSIMPAAYGCPPA 205 KP+ A VEVWHAN G YS FDP +Q PFN+R +I TD QGRY+FRS +P Y PP Sbjct: 140 KPLANALVEVWHANHLGRYSFFDP--DQSPFNLRCSIRTDEQGRYRFRSRVPVGYSVPPG 197 Query: 206 GPTQALLNQLGRHGNRPAHIHFFVTADGHRKLTTQINIDGDPLIYDDFAYATREGLVPPL 265 G T LL +LGRHG+RPAHIHFFVT G+RKLTTQINI GDP ++DDFA+ATREGLVP L Sbjct: 198 GATDRLLTKLGRHGSRPAHIHFFVTVPGYRKLTTQINIAGDPYLWDDFAFATREGLVPEL 257 Query: 266 VERTDAASIKAEGLEGPFAEIVFDMTLTSLVNGADNQIVDRPRLAA 311 TD A I+ ++ PF I F+ L ++RPRLAA Sbjct: 258 KHVTDPAEIRKHEVDAPFYAIEFNFNLLPEQADLPKADINRPRLAA 303 >gi|300313016|ref|YP_003777108.1| 6-chlorohydroxyquinol-1,2-dioxygenase [Herbaspirillum seropedicae SmR1] Length = 296 Score = 124 bits (311), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 80/272 (29%), Positives = 131/272 (48%), Gaps = 14/272 (5%) Query: 29 RVKQIVHRILSDLFKTIEDLEITSDEYWAGVAYINRLGTAS-----EGGLLSPGLGLDRF 83 R+++I+ ++ L ++++T E++AG+ ++ G E LLS LGL Sbjct: 32 RLREIMTSLVQHLHAFAREVKLTEKEWFAGIDFLTETGQICSDQRQEFILLSDTLGLSTL 91 Query: 84 LDMRMDAEDAAQGITGGTPRTIEGPLYVAGAPVEQGFARLDDGSDKNGHTIIMHGTIYGA 143 + + + + A G T T+ GP +V AP A + +G+ G + G++ G Sbjct: 92 VMAQQNHKPA-----GCTEATVFGPFHVEDAPQYPLGADIANGA--KGEPCFVSGSVRGR 144 Query: 144 DGKPMPGAQVEVWHANTKGFYSHFDPTGEQKPFNMRRTIITDAQGRYKFRSIMPAAYGCP 203 +G+P+ A++EVW A+ GFY + + R T+ TDA+GRY FRSI+ Y P Sbjct: 145 NGRPLAHARMEVWQADENGFYD--VQYADLDQYRARGTLFTDAEGRYHFRSILANPYPIP 202 Query: 204 PAGPTQALLNQLGRHGNRPAHIHFFVTADGHRKLTTQINIDGDPLIYDDFAYATREGLVP 263 GP +L +LGRH RPAH+HF + A+G+ L T + D + D + R L+ Sbjct: 203 HDGPVGRMLEKLGRHPWRPAHLHFRIQAEGYETLITHVFRRDDVYLDSDAVFGVRSTLIA 262 Query: 264 PLVERTDAASIKAEGLEGPFAEIVFDMTLTSL 295 + E PF + +D L L Sbjct: 263 DWPCHPAGTAPDGTQCEQPFYTLDYDFVLNPL 294 >gi|300313273|ref|YP_003777365.1| protocatechuate 3,4-dioxygenase beta chain protein [Herbaspirillum seropedicae SmR1] Length = 230 Score = 60.1 bits (144), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 45/136 (33%), Positives = 60/136 (44%), Gaps = 33/136 (24%) Query: 131 GHTIIMHGTIYGADGKPMPGAQVEVWHANTKGFYSH--------FDPTGEQKPFNMRRTI 182 G I++ G + DGKP+ + +EVW N G Y H DP F + Sbjct: 68 GEKIVVTGRVVDEDGKPVRNSLLEVWQCNAAGRYFHKKDQHDAPLDPN-----FTGFGKM 122 Query: 183 ITDAQGRYKFRSIMPAAYGCPPAGPTQALLNQLGRHGN--RPAHIHFFVTADGH-RKLTT 239 +TD GRY+F SI P Y G H RPAHIHF + + + ++L T Sbjct: 123 LTDDNGRYRFVSIKPGPY-------------PWGNHHKAWRPAHIHFSLFGNVYAQRLVT 169 Query: 240 QINIDGDPLIYDDFAY 255 Q+ DPL FAY Sbjct: 170 QMYFPNDPL----FAY 181 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.139 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,041,184 Number of extensions: 45631 Number of successful extensions: 118 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 114 Number of HSP's successfully gapped: 4 Length of query: 311 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 226 Effective length of database: 1,217,395 Effective search space: 275131270 Effective search space used: 275131270 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)