BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1124.p (403 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310638|ref|YP_003774730.1| muconate cycloisomerase [Herbas... 743 0.0 gi|300312959|ref|YP_003777051.1| muconate and chloromuconate cyc... 510 e-146 gi|300310475|ref|YP_003774567.1| mandelate racemase/muconate lac... 68 1e-12 gi|300310362|ref|YP_003774454.1| galactonate dehydratase [Herbas... 59 7e-10 >gi|300310638|ref|YP_003774730.1| muconate cycloisomerase [Herbaspirillum seropedicae SmR1] Length = 398 Score = 743 bits (1919), Expect = 0.0, Method: Compositional matrix adjust. Identities = 371/400 (92%), Positives = 386/400 (96%), Gaps = 2/400 (0%) Query: 4 MDTSQHLHTAAAPAPALIERVETFLVDLPTIRPHQLSMATMNGQTLMLVRLYCSDGSVGV 63 M+T+ LH A PALI+RVETFLVDLPTIRPHQLSMATMNGQTLMLVRLYCSDGS+GV Sbjct: 1 METT--LHNITAAGPALIDRVETFLVDLPTIRPHQLSMATMNGQTLMLVRLYCSDGSIGV 58 Query: 64 GEGTTIGGLAYGAESPEGMKLAIDTYFAPLLLGADASLIPAIMARLNKAIQDNRFAKCAI 123 GEGTTIGGLAYGAESPEGMKLAIDTYFAPLLLGADAS IPAIMARLNKAI DNRFAKCAI Sbjct: 59 GEGTTIGGLAYGAESPEGMKLAIDTYFAPLLLGADASQIPAIMARLNKAICDNRFAKCAI 118 Query: 124 ETALFDALGQRTGLPVSQLLGGRARESLPIAWTLASGDTAKDIDEAERMLAERRHNIFKL 183 ETALFDALGQRTGLPVSQLLGGR+RESLPIAWTLASGDTAKDIDEAERMLA+RRHNIFKL Sbjct: 119 ETALFDALGQRTGLPVSQLLGGRSRESLPIAWTLASGDTAKDIDEAERMLAQRRHNIFKL 178 Query: 184 KIGRREVEADVAHVAAIKKALGERGAVRVDVNMAWSELEAQRGLAGLVDAGCELVEQPVA 243 KIGRREV+ADVAHVAAIKKALG+RGAVRVDVNMAWSELEAQRGLAGLVDAGCELVEQPVA Sbjct: 179 KIGRREVDADVAHVAAIKKALGDRGAVRVDVNMAWSELEAQRGLAGLVDAGCELVEQPVA 238 Query: 244 SVQALARLKGKHPIAVMADESLTGPASAFALARIGGADVFAIKIEQSGGLRAAQQVAAIG 303 S QALARLKG+H IA+MADESLTGPASAFALARIGGADVFAIKIEQSGGLRAAQQVAAIG Sbjct: 239 SPQALARLKGRHAIAIMADESLTGPASAFALARIGGADVFAIKIEQSGGLRAAQQVAAIG 298 Query: 304 DAAGIELYGGTMLEGAIGTIASAHAFATFRELQWGTELFGPLLLTEEILATPLQYKDFQL 363 DAAGIELYGGTMLEGAIGTIASAHAFATFRELQWGTELFGPLLLTEEILA PLQY+DFQL Sbjct: 299 DAAGIELYGGTMLEGAIGTIASAHAFATFRELQWGTELFGPLLLTEEILAEPLQYQDFQL 358 Query: 364 AIPNGPGLGIVLDEERVQYFRRDRKQGATITAVRPAQLAG 403 AIP+ PGLGI LDEER+QYFRRDRKQGAT+TAVRPA+LAG Sbjct: 359 AIPHRPGLGITLDEERLQYFRRDRKQGATVTAVRPAKLAG 398 >gi|300312959|ref|YP_003777051.1| muconate and chloromuconate cycloisomerase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 510 bits (1313), Expect = e-146, Method: Compositional matrix adjust. Identities = 254/370 (68%), Positives = 297/370 (80%), Gaps = 2/370 (0%) Query: 21 IERVETFLVDLPTIRPHQLSMATMNGQTLMLVRLYCSDGSVGVGEGTTIGGLAYGAESPE 80 IE VE L+D+PTIRPH+LSM TM+ QTL+LVR+ +DG G GE TTIGGLAYG ESPE Sbjct: 10 IETVEVLLLDVPTIRPHRLSMTTMHRQTLVLVRITGNDGVAGWGEATTIGGLAYGEESPE 69 Query: 81 GMKLAIDTYFAPLLLGADASLIPAIMARLNKAIQDNRFAKCAIETALFDALGQRTGLPVS 140 +K+ IDTY PLLLGADA+ + A M +++ +Q NRFAKCAIETAL DAL +R G+P+S Sbjct: 70 SIKVNIDTYITPLLLGADANQVAARMHHIDEHVQGNRFAKCAIETALSDALARRLGVPLS 129 Query: 141 QLLGGRARESLPIAWTLASGDTAKDIDEAERMLAERRHNIFKLKIGRREVEADVAHVAAI 200 L GGR R+SLPIAWTLASGDTA+DIDEAE+MLA+RRHN+FKLKIG R V+ D AHVAAI Sbjct: 130 TLFGGRVRDSLPIAWTLASGDTARDIDEAEKMLAQRRHNLFKLKIGARTVQQDCAHVAAI 189 Query: 201 KKALGERGAVRVDVNMAWSELEAQRGLAGLVDAGCELVEQPVASVQ--ALARLKGKHPIA 258 K+ALGERG+VRVDVN AW+E EA G+ L DAG +LVEQP+A+ L RL+ K+ IA Sbjct: 190 KRALGERGSVRVDVNQAWTETEAMLGMQWLADAGVDLVEQPIAASNHDGLRRLRQKNRIA 249 Query: 259 VMADESLTGPASAFALARIGGADVFAIKIEQSGGLRAAQQVAAIGDAAGIELYGGTMLEG 318 +MADESL GP A+ LAR G ADVFA+KI QSGGL AQQVAAI AA +ELYGGTMLEG Sbjct: 250 IMADESLHGPQDAYQLARAGAADVFAVKINQSGGLAGAQQVAAIASAAQVELYGGTMLEG 309 Query: 319 AIGTIASAHAFATFRELQWGTELFGPLLLTEEILATPLQYKDFQLAIPNGPGLGIVLDEE 378 AIGT+ASA FATF L WG+ELFGPLLLTEEILATPL+Y+DF L +P GPGLGI LDE+ Sbjct: 310 AIGTMASAQLFATFGTLAWGSELFGPLLLTEEILATPLRYRDFALELPTGPGLGITLDED 369 Query: 379 RVQYFRRDRK 388 RV RRDRK Sbjct: 370 RVNALRRDRK 379 >gi|300310475|ref|YP_003774567.1| mandelate racemase/muconate lactonizing [Herbaspirillum seropedicae SmR1] Length = 424 Score = 68.2 bits (165), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 74/306 (24%), Positives = 130/306 (42%), Gaps = 27/306 (8%) Query: 96 GADASLIPAIMARLNKAIQDNRFAKCAIETALFDALGQRTGLPVSQLLGGRARESLPIAW 155 A A L P A +KA+ + A A E A DA + +P+ LLGG R+ +P + Sbjct: 92 NAGAELAPGSHA--SKAVSN---AYSAFEVACLDAQARYLNVPLVDLLGGAVRKEIPFSA 146 Query: 156 TL---------------ASGDTAKD---IDEAERMLAERRHNIFKLKIGRREVEADVAHV 197 L A G+ + + +A +M+ E KLK G E +VA + Sbjct: 147 YLFFKYAQHIDSPYAPDAWGEALSEEQIVAQARKMIEENGFQSIKLKAGALAPEHEVACI 206 Query: 198 AAIKKALGERGAVRVDVNMAWSELEAQRGLAGLVDAGCELVEQPVASVQALARLKGKHPI 257 A++KA + +R+D N WS A R +A L+ + E P ++ +A L + + Sbjct: 207 KALRKAFPD-APLRIDPNGNWSLQTAIR-MAELLGDDLQYYEDPTPGLEGMAELHRRTGL 264 Query: 258 AVMADESLTGPASAFALARIGGADVFAIKIEQSGGLRAAQQVAAIGDAAGIELYGGTMLE 317 + + +T + + GGLR QQ+A + D G+ + + Sbjct: 265 PLATNMVVTDFDELRRSVALDSVQIVLADHHYWGGLRDTQQLAKMCDVFGLGVSMHSNSH 324 Query: 318 GAIGTIASAHAFATFRELQWGTELFGPLLLTEE--ILATPLQYKDFQLAIPNGPGLGIVL 375 I +A +H A L + + P +E I L ++ + I + PGLG+ + Sbjct: 325 LGISLMAMSHVAAAVENLSYACDTHYPWQEPDEEVIQGGKLPIRNGCVQITDAPGLGVEV 384 Query: 376 DEERVQ 381 D+++++ Sbjct: 385 DQDQLR 390 >gi|300310362|ref|YP_003774454.1| galactonate dehydratase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 58.9 bits (141), Expect = 7e-10, Method: Compositional matrix adjust. Identities = 77/287 (26%), Positives = 123/287 (42%), Gaps = 34/287 (11%) Query: 119 AKCAIETALFDALGQRTGLPVSQLLGGRARESLPIAWTLASGDTAKDIDEAERMLAERRH 178 A I+ AL+D G+ G+PV QLLGG R S+ + ++ GD D A + R Sbjct: 82 ALAGIDQALWDIKGKDLGVPVHQLLGGAVRNSIRV-YSWIGGDRPADTAAAAKSAVARGF 140 Query: 179 NIFKLK--------IGRREVEADVAHVAAIKKALGERGAVRVDVNMAWSELEAQRGLAGL 230 K+ ++E +A+V A+++A+G + VD + + A+ + L Sbjct: 141 TAVKMNGTEELQFIDSHDKIELTLANVQAVREAVGPNVGIGVDFHGRVHKPMAKALIKEL 200 Query: 231 VDAGCELVEQPVASVQALARLKGKH----PIAVMADESLTGPASAFALARI---GGADVF 283 +E+PV S A A + H PIA+ E L S + RI G D+ Sbjct: 201 EPYKLMFIEEPVLSENAEALKEIAHLTSTPIAL--GERLY---SRWDFKRILSEGYVDII 255 Query: 284 AIKIEQSGGLRAAQQVAAIGDAAGIELYGGTMLEGAIGTIASAHAF--------ATFREL 335 + +GG+ +++A + +A + L L +G IA A A +E Sbjct: 256 QPDVSHAGGITETRKIAMMAEAYDVAL----ALHCPLGPIALASCLQVDAGSYNAFIQEQ 311 Query: 336 QWGTELFGPLLLTEEIL-ATPLQYKDFQLAIPNGPGLGIVLDEERVQ 381 G L + I Y+D + IP GPGLGI ++EE V+ Sbjct: 312 SLGIHYNESNDLLDYIRDKNVFAYEDGYVKIPQGPGLGIEINEEYVR 358 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,229,820 Number of extensions: 52049 Number of successful extensions: 236 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 233 Number of HSP's successfully gapped: 4 Length of query: 403 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 316 Effective length of database: 1,207,925 Effective search space: 381704300 Effective search space used: 381704300 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)